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- 20.109(F15):DNA engineering using PCR (Day1) (13:47, 2 October 2015)
- 20.109(F15):FACS analysis (Day7) (14:29, 2 October 2015)
- 20.309: Exam 1 Topics (03:12, 5 October 2015)
- 20.109(F15): DNA engineering summary (16:41, 7 October 2015)
- 20.109(F15):Prepare expression system (Day2) (20:42, 14 October 2015)
- 20.109(F15):20.109 Class Blog summary page (13:06, 15 October 2015)
- Shot noise and centroid finding (13:59, 15 October 2015)
- 20.109(F15): DNA engineering presentation (13:23, 17 October 2015)
- Real electronics (16:28, 20 October 2015)
- 20.109(F15):Induce protein and evaluate DNA (Day3) (20:22, 21 October 2015)
- Manta G032 camera measurements (00:26, 22 October 2015)
- 20.109(F15):Assess protein function (Day7) (00:24, 3 November 2015)
- 20.109(F15):Journal Club II (Day8) (04:42, 3 November 2015)
- 20.109(F15): TA notes for module 2 (12:11, 3 November 2015)
- 20.109(F15):Phage nanowires (Day2) (18:32, 3 November 2015)
- 20.109(F15) (14:08, 4 November 2015)
- 20.109(F15): Protein engineering report (18:12, 9 November 2015)
- 20.109(F15):Characterize protein expression (Day6) (21:42, 10 November 2015)
- 20.109(F15):Purify protein (Day4) (18:01, 11 November 2015)
- 20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1) (14:45, 12 November 2015)
- 20.109(F15):Growth of phage materials (Day1) (14:26, 16 November 2015)
- 20.109(F15):Research proposal presentations (Day6) (18:12, 16 November 2015)
- SToNCalculator.m (18:45, 16 November 2015)
- 20.109(F15):Purify active materials (Day2) (18:56, 17 November 2015)
- 20.109(F15):Cathode construction (Day3) (21:55, 18 November 2015)
- 20.109(F15): Assignments (22:06, 25 November 2015)
- 20.109(F15):TEM (Day4) (17:07, 28 November 2015)
- 20.109(F15): Biomaterials engineering report (17:06, 30 November 2015)
- 20.109(F15):Research proposal presentation (18:50, 30 November 2015)
- 20.109(F15):Battery assembly and testing (Day5) (18:31, 2 December 2015)
- 20.109(F15):Homework (20:04, 2 December 2015)
- 20.109(F15): Fall 2015 schedule (21:00, 6 December 2015)
- 20.109(S16):Statement on collaboration and integrity (14:49, 9 December 2015)
- 20.109(S16):Guidelines for maintaining your lab notebook (16:04, 9 December 2015)
- 20.109(S16):Guidelines for oral presentations (16:07, 9 December 2015)
- 20.109(S16):Lab basics (16:08, 9 December 2015)
- 20.109(S16):General lab policy (16:09, 9 December 2015)
- 20.109(S16):Guidelines for working in the tissue culture facility (16:21, 9 December 2015)
- 20.109(S16):OWW basics (16:25, 9 December 2015)
- 20.109(S16):Cathode construction (Day3) (22:07, 10 December 2015)
- 20.109(F15): TA's notes for module 3 (14:26, 11 December 2015)
- 20.109(F15):Module 3 (15:14, 11 December 2015)
- 20.109(S16): TA notes for module 3 (15:15, 11 December 2015)
- Estimating second order system parameters from noise power spectra using nonlinear regression (21:47, 27 December 2015)
- Lab Manual: Limits of Detection (17:08, 4 January 2016)
- 20.109(S16):Student registration and questionnaire (21:27, 5 January 2016)
- 20.109(S16):Journal Club I (Day4) (18:44, 6 January 2016)
- 20.109(S16):Research proposal presentations (Day6) (18:50, 6 January 2016)
- 20.109(F15): TA notes for module 1 (17:19, 12 January 2016)
- 20.109(S16): TA notes for orientation (21:01, 27 January 2016)
- 20.109(S16):People (21:00, 31 January 2016)
- Temporary Optics Bootcamp (21:32, 2 February 2016)
- 20.109(S16):Lab tour (13:50, 4 February 2016)
- 20.109(S16):In situ cloning (Day1) (21:29, 4 February 2016)
- 20.109(S16):Module 2 (16:45, 5 February 2016)
- 20.109(S16):Design mutation primers (Day2) (20:41, 9 February 2016)
- 20.109(S16): Protein engineering presentation (19:42, 15 February 2016)
- 20.109(S16):Site-directed mutagenesis (Day3) (14:08, 17 February 2016)
- 20.109(S16):Prepare expression system (Day4) (22:14, 21 February 2016)
- Particle Tracking Exercise (19:23, 22 February 2016)
- 20.109(S16):Induce protein expression (Day5) (21:55, 23 February 2016)
- 20.109(S16):Characterize protein expression (Day7) (14:39, 26 February 2016)
- 20.109(S16):Purify protein (Day6) (20:15, 26 February 2016)
- 20.109(S16): Protein engineering summary (20:18, 29 February 2016)
- 20.109(S16): TA notes for module 1 (17:12, 2 March 2016)
- 20.109(S16):Assess protein function (Day8) (20:14, 2 March 2016)
- 20.109(S16):Module 1 (15:19, 6 March 2016)
- Optical Microscopy: Part 3 Report Outline (19:04, 7 March 2016)
- Optical Microscopy Part 4: Particle Tracking (01:01, 10 March 2016)
- 20.109(S16):Begin Western blot protein analysis and choose system conditions (Day2) (15:00, 11 March 2016)
- Optical Microscopy: Part 4 Report Outline (19:44, 14 March 2016)
- 20.109(S16): TA notes for module 2 (18:04, 16 March 2016)
- 20.109(S16):Cell preparation for DNA repair assays (Day5) (18:34, 28 March 2016)
- 20.109(S16):20.109 Class Blog summary page (19:24, 28 March 2016)
- 20.109(S16):Complete Western and prepare damaged DNA (Day3) (15:49, 1 April 2016)
- 20.109(S16):Journal Club II (Day8) (18:50, 2 April 2016)
- 20.109(S16):Flow cytometry and paper discussion (Day7) (13:19, 4 April 2016)
- 20.109(S16): Assignments (18:05, 6 April 2016)
- 20.109(S16):Guidelines for writing up your research (15:35, 7 April 2016)
- 20.109(S16):Module 3 (19:48, 9 April 2016)
- 20.109(S16):Data analysis (Day9) (16:31, 11 April 2016)
- Lab Manual: Measuring DNA Melting Curves (03:39, 13 April 2016)
- 20.109(S16):DNA repair assays (Day6) (14:32, 14 April 2016)
- 20.109(S16): System engineering report (16:17, 14 April 2016)
- 20.109(S16):Growth of phage materials (Day1) (18:48, 15 April 2016)
- 20.109(S16):TEM (Day4) (15:35, 19 April 2016)
- DNA melting lab wiki pages (14:33, 21 April 2016)
- 20.109(S16):Purify active materials (Day2) (21:58, 21 April 2016)
- 20.109(S16):Research proposal presentation (17:47, 25 April 2016)
- 20.109(S16): Biomaterials engineering report (16:55, 28 April 2016)
- 20.109(S16):Battery assembly and testing (Day5) (17:28, 5 May 2016)
- 20.109(S16):Homework (17:41, 5 May 2016)
- 20.109(S16) (17:54, 5 May 2016)
- 20.109(S16): Spring 2016 schedule (20:44, 6 May 2016)
- 20.109(F16):DNA engineering using PCR (Day1) (14:58, 18 May 2016)
- 20.109(F16):Clean and cut DNA (Day2) (15:11, 18 May 2016)
- 20.109(F16):Agarose gel electrophoresis (Day3) (15:16, 18 May 2016)
- 20.109(F16):DNA ligation & transformation (Day4) (15:25, 18 May 2016)
- 20.109(F16):Examine candidate clones & tissue culture (Day5) (15:28, 18 May 2016)
- 20.109(F16):Lipofection (Day6) (15:33, 18 May 2016)
- 20.109(F16):FACS analysis (Day7) (15:37, 18 May 2016)
- 20.109(F16):Cathode construction (Day3) (15:50, 18 May 2016)
- 20.109(F16):Statement on collaboration and integrity (19:37, 18 May 2016)
- 20.109(F16): DNA engineering summary (14:19, 9 June 2016)
- 20.109(F16): DNA engineering presentation (14:21, 9 June 2016)
- 20.109(F16):Test comet chip assay variables (Day3) (19:20, 18 July 2016)
- 20.109(F16):Journal club I (Day4) (14:55, 28 July 2016)
- Complex Number Review (15:40, 12 August 2016)
- Sandbox (21:42, 16 August 2016)
- BE TA Training(Su16):Homepage (14:32, 25 August 2016)
- Input and output impedance (16:35, 26 August 2016)
- 20.109(F09):People (21:59, 28 August 2016)
- 20.109(S10):Testing cell viability (Day3) (22:02, 28 August 2016)
- 20.109(S10):People (17:54, 29 August 2016)
- Measuring biological forces mini-lab (18:00, 29 August 2016)
- Spring 2012: Jonathan Gootenberg (18:02, 29 August 2016)
- 20.109(F16): TA notes for orientation (17:08, 7 September 2016)
- 20.109(F16): M1 Mini-presentation (18:57, 7 September 2016)
- 20.109(F16):Lab tour (21:13, 9 September 2016)
- Protocols for making microscopy samples (23:15, 12 September 2016)
- 20.109(F16):Prepare comet chips (Day1) (17:32, 19 September 2016)
- 20.109(S16):Introduction to cell strains and plating (Day1) (17:34, 19 September 2016)
- 20.109(F16):Test comet chip loading variables (Day2) (17:33, 22 September 2016)
- 20.109(F16):Module 1 (13:43, 23 September 2016)
- 20.109(F16):Measure DNA damage with comet chip (Day3) (19:35, 26 September 2016)
- 20.109(F16):Asses DNA repair variability with comet chip (Day4) (17:23, 27 September 2016)
- 20.109(F16): M1 Data Summary (12:08, 29 September 2016)
- Optical Microscopy Part 3: Resolution and Stability (18:12, 29 September 2016)
- 20.109(F16):Seed cells for immuno-flourescence assay (Day5) (20:08, 30 September 2016)
- 20.109(F16): Announcements (19:11, 3 October 2016)
- 20.109(F16):Complete immuno-fluorescence assay (Day6) (12:00, 5 October 2016)
- 20.109(F16): TA notes for M1 (13:14, 6 October 2016)
- 20.109(F16):Design gRNA for CRISPRi (Day2) (15:25, 6 October 2016)
- 20.109(F16):Data analysis (Day7) (16:39, 6 October 2016)
- 20.109(F16):Complete in silico cloning (Day1) (17:24, 13 October 2016)
- 20.109(F16): TA notes for M12 (18:34, 19 October 2016)
- MATLAB: Intensity-weighted centroid noise formula (17:58, 25 October 2016)
- 20.109(F16): Assignments (15:04, 1 November 2016)
- 20.109(F16):Journal club II (Day 6) (16:52, 2 November 2016)
- 20.109(F16):Induce CRISPRi system (Day7) (19:38, 3 November 2016)
- 20.109(F16):Module 2 (19:39, 3 November 2016)
- 20.109(F16): Research article (16:42, 4 November 2016)
- 20.109(F16):TEM (Day4) (18:35, 8 November 2016)
- 20.109(F16): TA notes for M2 (18:49, 8 November 2016)
- 20.109(F16)::Measure fermentation products (Day8) (15:22, 9 November 2016)
- 20.109(F16):Confirm gRNA sequence (Day5) (21:01, 10 November 2016)
- 20.109(F16):Generate gRNA plasmid (Day3) (20:42, 14 November 2016)
- 20.109(F16):Module 3 (16:19, 15 November 2016)
- 20.109(F16):Purify active materials (Day2) (18:40, 15 November 2016)
- 20.109(F16): TA notes for M3 (17:56, 16 November 2016)
- 20.109(F16):Growth of phage materials (Day1) (21:20, 16 November 2016)
- 20.109(F16):Research proposal presentation (15:22, 17 November 2016)
- 20.109(F16):Homework (21:26, 30 November 2016)
- 20.109(F16):Communication (22:00, 30 November 2016)
- 20.109(F16):Battery assembly and testing (Day5) (19:32, 5 December 2016)
- 20.109(F16): Biomaterials engineering mini-report (16:23, 6 December 2016)
- 20.109(F16): Fall 2016 schedule (18:39, 19 December 2016)
- 20.109(S17): Fall 2016 schedule (16:25, 27 December 2016)
- 20.109(S17):Research proposal presentation (19:40, 28 December 2016)
- 20.109(S17): TA notes for M3 (19:43, 28 December 2016)
- Lab Manual: Optical Microscopy (15:19, 10 January 2017)
- Microscopy report general guidelines (16:55, 11 January 2017)
- Microscopy report outline (17:04, 11 January 2017)
- Optical Microscopy Part 1: Brightfield Microscopy (22:45, 12 January 2017)
- 20.309:DAQ System (16:19, 17 January 2017)
- 20.109(S17): M1 Mini-presentation (01:27, 31 January 2017)
- Creating 20.109(S17):Scan slides to identify FKBP12 binders (Day5) (17:43, 31 January 2017)
- 20.109(S17):Lab tour (21:35, 31 January 2017)
- 20.109(S17):Cathode construction (Day3) (22:17, 31 January 2017)
- 20.109(S17):TEM (Day4) (22:18, 31 January 2017)
- 20.109(S17):Journal Club I (Day5) (00:27, 1 February 2017)
- 20.109(S17):Examine transcript levels in response to DNA damage (Day 4) (02:33, 3 February 2017)
- 20.109(S17): M1 Data Summary (15:32, 7 February 2017)
- 20.109(S17):Module 1 (17:11, 7 February 2017)
- 20.109(S17):Module 3 (17:12, 7 February 2017)
- 20.109(S17):Assess cell survival and harvest RNA for quantitative PCR assay (Day3) (01:48, 9 February 2017)
- 20.109(S17): Assignments (19:22, 10 February 2017)
- 20.109(S17): Announcements (22:17, 10 February 2017)
- 20.109(S17):Purification of induced protein (Day2) (14:22, 23 February 2017)
- 20.109(S17):Scan slides to identify FKBP12 binders (Day5) (19:49, 27 February 2017)
- 20.109(S17):Screen chemical library for FKBP12 binders (Day4) (21:59, 27 February 2017)
- 20.109(S17):Evaluation of purified protein (Day3) (21:33, 28 February 2017)
- 20.109(S17):In silico cloning and induction of protein expression (Day1) (19:53, 2 March 2017)
- 20.109(S17):Complete data analysis (Day6) (16:05, 3 March 2017)
- 20.109(S17):Identify chemical structures common among FKBP12 binders (15:52, 6 March 2017)
- 20.109(S17):Complete Western blot and induce DNA damage for survival and quantitative PCR assays (Day2) (01:02, 7 March 2017)
- 20.109(S17):Confirm cell lines and practice tissue culture techniques (Day1) (21:06, 9 March 2017)
- 20.309:Lab Report Guidelines (21:33, 13 March 2017)
- Part 3 Combined Report Outline (16:00, 14 March 2017)
- Optical Microscopy Part 3: Resolution, Stability, and Particle Tracking (22:47, 15 March 2017)
- 20.109(S17):Prepare Western blot and induce DNA damage for quantitative PCR assay (Day2) (17:15, 17 March 2017)
- 20.109(S17):Complete cell survival assay and examine transcript levels in response to DNA damage (Day 4) (17:55, 22 March 2017)
- DNA Melting Report Requirements for Part 1 (21:34, 23 March 2017)
- 20.109(S17):Practice data analysis methods (Day6) (01:35, 3 April 2017)
- 20.109(S17): Research article (00:09, 4 April 2017)
- 20.109(S17):Analyze RNA-seq data (Day7) (15:16, 4 April 2017)
- 20.109(S17):Journal Club II (Day8) (20:50, 6 April 2017)
- 20.109(S17):Module 2 (18:04, 7 April 2017)
- Electronics Mini-Lab (18:46, 7 April 2017)
- 20.109(S17):Examine quantitative PCR results (Day9) (15:44, 12 April 2017)
- DNA Melting Report Requirements (18:50, 16 April 2017)
- DNA Melting Part 1: Measuring Temperature and Fluorescence (17:49, 19 April 2017)
- 20.109(S17):Growth of phage materials (Day1) (14:28, 24 April 2017)
- 20.109(S17):Purify active materials (Day2) (14:34, 24 April 2017)
- 20.109(S17): Biomaterials engineering mini-report (16:51, 27 April 2017)
- 20.109(S17):Communication (17:29, 28 April 2017)
- 20.109(S17):Battery assembly and testing (Day5) (14:18, 9 May 2017)
- 20.109(S17): Spring 2017 schedule (19:53, 10 May 2017)
- 20.109(F17): Notes for orientation (17:17, 13 July 2017)
- Bode plots (20:05, 31 July 2017)
- Optics Bootcamp (20:09, 31 July 2017)
- 20.309 Assignment 1: Optics bootcamp (20:26, 8 August 2017)
- Optical Microscopy Part 2: Fluorescence Microscopy (22:06, 14 August 2017)
- DNA Melting: Simulating DNA Melting - Intermediate Topics (21:46, 17 August 2017)
- Impedance Analysis (21:49, 17 August 2017)
- DNA Melting: Model function and parameter estimation by nonlinear regression (21:55, 17 August 2017)
- 20.109(F17):Journal club I (Day4) (14:30, 22 August 2017)
- 20.109(F17):Visualize active material using TEM (Day4) (16:53, 22 August 2017)
- 20.109(F17):Cathode construction (Day3) (17:02, 22 August 2017)
- Understanding log plots (13:13, 23 August 2017)
- Assignment 2: epi illuminator for fluorescence microscopy (14:06, 25 August 2017)
- BE TA Training (Su17) (13:59, 28 August 2017)
- 20.109(S17):Guidelines for working in the tissue culture facility (15:51, 6 September 2017)
- 20.109(S17): TA notes for orientation (16:07, 6 September 2017)
- 20.109(F17): M1 Mini-presentation (19:30, 6 September 2017)
- 20.109(F17):Module 1 (16:31, 8 September 2017)
- 20.109(F17): Notes for M1 (18:12, 11 September 2017)
- Assignment 2 Part 4: Fluorescent imaging of actin (03:21, 14 September 2017)
- 20.109(F17):Lab tour (13:25, 14 September 2017)
- 20.109(F17):Develop experiment to optimize loading variables (Day2) (14:28, 15 September 2017)
- 20.109(F17): Announcements (17:13, 15 September 2017)
- 20.109(F17):Evaluate cell loading results (Day3) (20:41, 19 September 2017)
- 20.109(F17):Prepare comet chips (Day1) (20:42, 19 September 2017)
- 20.109(F17):Test role of biochemical factors in genomic stability (Day4) (21:55, 21 September 2017)
- 20.109(F17):Complete biochemical experiment (Day5) (12:41, 29 September 2017)
- MATLAB: Estimating resolution from a PSF slide image (14:21, 30 September 2017)
- 20.109(F17):Examine sub-nuclear foci abundance to measure DNA damage (Day6) (15:22, 4 October 2017)
- 20.109(F17):Module 2 (22:28, 9 October 2017)
- 20.109(F17):Visualize and analyze data for sub-nuclear foci assay (Day7) (17:57, 11 October 2017)
- 20.109(F17): M1 Data Summary (19:31, 11 October 2017)
- 20.109(F17):Complete in silico cloning (Day1) (19:34, 13 October 2017)
- 20.109(F17):Design gRNA for CRISPRi (Day2) (20:47, 17 October 2017)
- 20.109(F17):Generate gRNA plasmid (Day3) (16:32, 19 October 2017)
- 20.109(F17):Journal club II (Day 6) (05:39, 20 October 2017)
- Assignment 7, Part 3: testing your instrument and measuring a DNA melting curve (18:17, 27 October 2017)
- 20.109(F17):Confirm gRNA sequence (Day5) (18:35, 27 October 2017)
- 20.109(F17):Homework (19:34, 27 October 2017)
- 20.109(S17):Purify RNA for quantitative PCR assay and treat cells for survival assay (Day3) (19:11, 30 October 2017)
- 20.109(F17):Induce CRISPRi system (Day7) (20:39, 30 October 2017)
- Assignment 8, Part 1: convolution (17:40, 5 November 2017)
- 20.109(F17)::Measure fermentation products (Day8) (19:46, 7 November 2017)
- DNA Melting Part 2: Lock-in Amplifier and Temperature Control (20:05, 7 November 2017)
- Assignment 8, Part 2: lock-in amplifier and temperature control (22:56, 7 November 2017)
- DNA Melting: Using the Basic DNAMelter GUI (23:46, 7 November 2017)
- DNA Melting: Using the LockIn DNAMelter GUI (00:16, 8 November 2017)
- DNA Melting: DNA Sequences (00:03, 10 November 2017)
- Understanding the lock-in amplifier (16:36, 13 November 2017)
- 20.109(F17):Module 3 (19:03, 13 November 2017)
- Assignment 9, Part 1: model function (15:29, 14 November 2017)
- 20.109(F17):Growth of phage materials (Day1) (16:31, 14 November 2017)
- 20.109(F17): Research article (16:23, 15 November 2017)
- 20.109(F17):Purify active materials (Day2) (19:28, 16 November 2017)
- 20.109(F17):Research proposal presentation (18:49, 17 November 2017)
- 20.109(F17): Biomaterials engineering mini-report (18:53, 17 November 2017)
- 20.109(F17):Communication (15:37, 20 November 2017)
- DNA melting: Identifying the unknown sample (18:49, 28 November 2017)
- 20.109(F17): Assignments (16:16, 30 November 2017)
- 20.109(F17):Assemble and test batteries (Day5) (17:08, 5 December 2017)
- 20.109(F17): Fall 2017 Schedule (20:44, 6 December 2017)
- Lab Manual: Atomic Force Microscopy (AFM) (20:42, 13 December 2017)
- 20.109(S18):Visualize phage nanowires using TEM (Day4) (02:56, 5 January 2018)
- 20.109(S18): Biomaterials engineering mini-report (15:59, 6 January 2018)
- 20.109(S18): M1 Mini-presentation (19:10, 6 January 2018)
- 20.109(S18):Research proposal presentation (21:19, 10 January 2018)
- 20.109(S18): TA notes for orientation (20:59, 16 January 2018)
- Assignment 9 Overview (00:20, 25 January 2018)
- 20.109(S18):SECONDARY ASSAY D2 (Day6) (18:36, 25 January 2018)
- 20.109(S18):Module 1 (19:04, 25 January 2018)
- 20.109(S18):Module 2 (19:13, 25 January 2018)
- 20.109(S18):Complete data analysis (Day7) (21:44, 25 January 2018)
- Assignment 8 Overview (18:43, 29 January 2018)
- 20.109(S18): Research article (18:31, 31 January 2018)
- 20.345:Course Information (17:22, 6 February 2018)
- 20.109(S18):Laboratory tour (21:39, 6 February 2018)
- 20.109(S18):Analyze small microarray data and induce protein expression (Day2) (21:15, 16 February 2018)
- 20.109(S18):In silico cloning and confirmation digest of protein expression vector(Day1) (21:17, 16 February 2018)
- 20.109(S18):Evaluate protein purity and concentration (Day4) (20:02, 26 February 2018)
- 20.109(S18): Prep notes for M1 (14:53, 1 March 2018)
- 20.109(S18):Purify protein for secondary assays(Day3) (01:13, 6 March 2018)
- 20.109(S18):Test protein activity using peptidyl-prolyl cis-trans isomerase assay (Day5) (12:53, 7 March 2018)
- 20.109(S18):Confirm ligand binding using differential scanning fluorimetry assay (Day6) (13:16, 8 March 2018)
- 20.109(S18): Prep notes for M2 (15:47, 8 March 2018)
- 20.109(S18): M1 Data Summary (16:05, 10 March 2018)
- 20.109(S18): Announcements (21:41, 11 March 2018)
- 20.109(S18):Induce DNA damage and apply drug treatments for RNA purification (Day2) (14:24, 14 March 2018)
- 20.109(S18):Practice tissue culture techniques and prepare cells for RNA purification (Day1) (13:44, 20 March 2018)
- 20.109(S18):Analyze RNA-seq data and prepare for quantitative PCR experiment (Day 4) (19:38, 21 March 2018)
- 20.109(S18):Investigate RNA-seq data using public databases (Day5) (16:09, 23 March 2018)
- Assignment 6, Part 1: Pre-lab questions (20:28, 3 April 2018)
- Assignment 7, Part 1: op amp golden rules questions (21:44, 3 April 2018)
- Assignment 7 Overview (21:49, 3 April 2018)
- Assignment 7, Part 2: measure temperature and fluorescence (17:42, 6 April 2018)
- 20.109(S18):Induce DNA damage and apply drug treatments for cell viability and identification of regulatory motifs in RNA-seq data (Day8) (02:15, 11 April 2018)
- 20.109(S17):Complete cell viability assay (Day9) (15:29, 13 April 2018)
- DNA Melting: Simulating DNA Melting - Basics (22:46, 15 April 2018)
- 20.109(S18):Prep notes for M3 (19:04, 17 April 2018)
- Assignment 9, Part 2: Simulating DNA melting data and testing the model function (21:02, 17 April 2018)
- 20.109(S18):Module 3 (15:13, 19 April 2018)
- 20.109(S18):Purify phage-based active (cathode) material (Day2) (16:41, 24 April 2018)
- 20.109(S18):Construct cathode with phage-based active material (Day3) (15:43, 27 April 2018)
- Assignment 9, Part 3: Fitting your data (22:39, 27 April 2018)
- 20.109(S18):Growth of phage-based active (cathode) material (Day1) (13:17, 30 April 2018)
- 20.109(S18):Communication (15:30, 1 May 2018)
- 20.109(S17):Homework (18:41, 3 May 2018)
- 20.109(S18):Homework (18:41, 3 May 2018)
- Assignment 10 Overview (16:37, 8 May 2018)
- 20.109(S18):Assemble and test batteries (Day5) (18:12, 9 May 2018)
- 20.109(S18): Spring 2018 schedule (02:26, 14 May 2018)
- 20.109(S18):Class data (10:06, 14 May 2018)
- 20.109(S18):Journal club presentations (Day6 and 7) (15:10, 28 June 2018)
- 20.109(F18):Construct cathode with phage-based active material (Day3) (15:47, 28 June 2018)
- 20.109(F18):Visualize phage nanowires using TEM (Day4) (15:48, 28 June 2018)
- 20.109(F18):Image sub-nuclear foci assay (15:34, 23 August 2018)
- 20.109(F18):Image sub-nuclear foci assay (Day6) (15:38, 23 August 2018)
- 20.109(F18):Research proposal presentation (19:49, 23 August 2018)
- 20.109(F18): Mini-report (19:49, 23 August 2018)
- BE TA Training (Su18) (18:39, 28 August 2018)
- Using nonlinear regression to create a camera specification sheet (20:23, 29 August 2018)
- 20.109(S18): Assignments (19:30, 31 August 2018)
- Error analysis (14:57, 3 September 2018)
- 20.109(F18):Evaluate cell loading experiment and treat cells for genomic instability experiment (Day3) (20:37, 4 September 2018)
- 20.109(F18):Module 2 (20:45, 4 September 2018)
- 20.109(F18):Module 3 (20:45, 4 September 2018)
- Optical microscopy lab wiki pages (12:49, 5 September 2018)
- Physical optics and resolution (14:22, 6 September 2018)
- 20.109(F18):Lab tour (19:28, 6 September 2018)
- Assignment 4: finding and measuring things (14:49, 17 September 2018)
- 20.109(F18):Prepare and treat cells for genomic instability experiment (Day3) (22:12, 20 September 2018)
- 20.109(F18):Module 1 (01:21, 21 September 2018)
- 20.109(F18):Complete genomic instability experiment and load cells for sub-nuclear foci assay (Day4) (15:12, 25 September 2018)
- 20.109(F18): Announcements (17:23, 25 September 2018)
- 20.109(F18):Design cell loading optimization experiment and discuss genomic instability experiment (Day2) (17:38, 25 September 2018)
- 20.109(F18):Prep notes for M3 (19:35, 25 September 2018)
- 20.109(F18): Prep notes for M2 (16:19, 26 September 2018)
- Assignment 3, Part 2: experimental design with fluorescence (17:51, 26 September 2018)
- 20.109(F18):Practice tissue culture and prepare microwell array (Day1) (18:35, 26 September 2018)
- 20.109(F18): Prep notes for M1 (18:50, 26 September 2018)
- 20.109(F18):Analyze genomic instability experiment data and treat cells for sub-nuclear foci assay (Day5) (18:29, 27 September 2018)
- Assignment 3 Overview (14:08, 1 October 2018)
- Assignment 6, Part 3: Identifying unknown filter circuits (17:07, 2 October 2018)
- 20.109(F18):Complete sub-nuclear foci assay (Day6) (18:24, 2 October 2018)
- 20.109(F18): Mini-presentation (20:02, 2 October 2018)
- 20.109(F18): Data Summary (20:03, 2 October 2018)
- 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7) (19:34, 4 October 2018)
- Assignment 7: Amplifiers and feedback (02:24, 10 October 2018)
- MSD of Sum and Difference Trajectories (15:35, 10 October 2018)
- Assignment 6 Overview (12:51, 14 October 2018)
- Assignment 6, Part 2: Electronics bootcamp (11:31, 15 October 2018)
- 20.109(F18):Generate gRNA plasmid (Day3) (20:11, 15 October 2018)
- Electronics primer (13:50, 19 October 2018)
- 20.109(F18):Journal club presentation (Day4 and 6) (19:15, 23 October 2018)
- 20.109(F18):Design gRNA for CRISPRi (Day2) (15:29, 25 October 2018)
- 20.109(F18):Confirm gRNA sequence (Day5) (18:26, 25 October 2018)
- 20.109(F18):Induce CRISPRi system (Day7) (16:46, 1 November 2018)
- 20.109(F18):Measure fermentation products (Day8) (19:55, 6 November 2018)
- 20.109(F18): Research article (01:33, 8 November 2018)
- 20.109(F18):Purify phage-based active (cathode) material (Day2) (18:13, 13 November 2018)
- 20.109(F18):Growth of phage-based active (cathode) material (Day1) (18:15, 13 November 2018)
- 20.109(F18):Communication (22:21, 16 November 2018)
- 20.109(F18):Assemble and test batteries (Day5) (20:24, 4 December 2018)
- 20.109(F18): Fall 2018 schedule (16:06, 5 December 2018)
- 20.109(F18): Assignments (16:07, 5 December 2018)
- 20.109(S19): Prep notes for M1 (17:38, 29 January 2019)
- 20.109(S19):Prep notes for M3 (17:52, 29 January 2019)
- 20.109(S19):Module 3 (17:52, 29 January 2019)
- 20.109(S19):Construct cathode with phage-based active material (Day3) (17:59, 29 January 2019)
- 20.109(S19):Visualize phage nanowires using TEM (Day4) (18:01, 29 January 2019)
- 20.109(S19): M1 Mini-presentation (18:14, 29 January 2019)
- 20.109(S19): Mini-presentation (18:16, 29 January 2019)
- 20.109(S19):Research proposal presentation (19:33, 29 January 2019)
- 20.109(S19): Mini-report (19:33, 29 January 2019)
- 20.109(S19):Complete data analysis (Day7) (15:55, 31 January 2019)
- 20.109(S19): Prep notes for M2 (17:26, 31 January 2019)
- Recording, displaying and saving images in MATLAB (18:41, 31 January 2019)
- Matlab: Scalebars (20:37, 31 January 2019)
- Review of probability concepts (21:12, 31 January 2019)
- 20.109(S19):Laboratory tour (15:43, 6 February 2019)
- 20.109(S19): Prep notes, orientation (15:50, 6 February 2019)
- 20.109(S19):Evaluate protein purity and concentration (Day4) (14:41, 22 February 2019)
- 20.109(S19):Module 1 (16:35, 24 February 2019)
- 20.109(S19):Purify protein for secondary assays(Day2) (19:34, 25 February 2019)
- 20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1) (19:34, 25 February 2019)
- 20.109(S19): Announcements (17:01, 28 February 2019)
- 20.109(S19):Test protein activity using peptidyl-prolyl cis-trans isomerase assay (Day5) (19:08, 5 March 2019)
- 20.109(S19):Confirm ligand binding using differential scanning fluorimetry assay (Day6) (20:09, 5 March 2019)
- 20.109(S19):Guidelines for working in the tissue culture facility (23:28, 6 March 2019)
- 20.109(S19):Module 2 (00:26, 7 March 2019)
- 20.109(S18):Analyze small microarray data (Day3) (17:14, 7 March 2019)
- 20.109(S19): Data Summary (14:19, 12 March 2019)
- 20.109(S18):Purify RNA and practice RNA-seq data analysis methods (Day3) (00:38, 19 March 2019)
- 20.109(S19):Analyze RNA-seq data and prepare for quantitative PCR experiment (Day 4) (13:39, 19 March 2019)
- 20.109(S19):Etoposide treat cells for RNA purification (Day2) (16:16, 19 March 2019)
- 20.109(S19):Perform quantitative PCR experiment and explore additional RNA-seq datasets (Day5) (20:06, 20 March 2019)
- 20.109(S19):Design cell viability experiment and complete RNA-seq data analysis (Day6) (14:46, 2 April 2019)
- 20.109(S19):Journal club presentations (Day7 and 8) (00:58, 4 April 2019)
- 20.109(S19):Communication (21:14, 7 April 2019)
- 20.109(S19):Complete data analysis (Day9) (14:48, 9 April 2019)
- Using tfest to find a system model (22:03, 9 April 2019)
- 20.109(S19): Assignments (20:47, 10 April 2019)
- Electronics written problems (18:45, 15 April 2019)
- 20.109(S19):Purify RNA and practice RNA-seq data analysis methods (Day3) (20:29, 16 April 2019)
- 20.109(S19):Practice tissue culture techniques and prepare cells for RNA purification (Day1) (20:32, 16 April 2019)
- 20.109(S19):Growth of phage-based active (cathode) material (Day1) (16:36, 18 April 2019)
- 20.109(S19): Research article (19:40, 19 April 2019)
- 20.109(S19):Purify phage-based active (cathode) material (Day2) (17:00, 24 April 2019)
- 20.109(S19):Homework (01:02, 30 April 2019)
- 20.109(F18):Class data (17:43, 7 May 2019)
- 20.109(S19):Assemble and test batteries (Day5) (17:49, 7 May 2019)
- 20.109(S19): Spring 2019 schedule (19:16, 8 May 2019)
- 20.109(S19):Class data (20:04, 8 May 2019)
- Exam 2 study guide (12:16, 9 May 2019)
- 20.109(F19):Complete in silico cloning (Day1) (22:22, 7 August 2019)
- 20.109(F19):Guidelines for working in the tissue culture facility (18:28, 14 August 2019)
- 20.109(F19): Prep notes, orientation (18:36, 14 August 2019)
- 20.109(F19):Design gRNA sequence for CRISPRi (Day2) (18:52, 14 August 2019)
- 20.109(F19):Confirm gRNA sequence in pgRNA(Day5) (19:11, 14 August 2019)
- 20.109(F19):Module 2 (19:12, 14 August 2019)
- 20.109(F18):Homework (21:06, 21 August 2019)
- 20.109(F19): Prep notes for M2 (21:22, 22 August 2019)
- Lab orientation (14:49, 25 August 2019)
- Geometrical optics and ray tracing (01:20, 27 August 2019)
- 20.109(F19):Module 3 (18:21, 30 August 2019)
- 20.109(F19):Lab tour (20:37, 30 August 2019)
- 20.109(F19): Prep notes for M1 (17:48, 4 September 2019)
- Assignment 1, Part 1: Pre-lab questions (20:00, 5 September 2019)
- Assignment 1, Part 3: Building your transillumination microscope (19:23, 6 September 2019)
- 20.109(F19):Practice cell culture and begin sub-nuclear foci assay (Day1) (16:30, 9 September 2019)
- 20.109(F19): Announcements (18:51, 9 September 2019)
- 20.109(F19):Complete antibody staining for Western blot analysis (Day4) (22:58, 9 September 2019)
- 20.109(F19):Module 1 (22:27, 10 September 2019)
- BE TA Training (Su19) (14:21, 11 September 2019)
- 20.109(F18):Complete in silico cloning (Day1) (13:51, 12 September 2019)
- Assignment 2 Part 2: Fluorescence microscopy (18:39, 12 September 2019)
- 20.109(F19):Design gRNA for CRISPRi (Day2) (13:56, 13 September 2019)
- 20.109(F19):Treat cells for gamma-H2AX (Day2) (19:12, 17 September 2019)
- Flat-field correction (19:13, 18 September 2019)
- 20.109(F19):Generate gRNA plasmid (Day3) (20:44, 18 September 2019)
- 20.109(F19):Load cells into CometChip and apply treatments (Day4) (15:14, 24 September 2019)
- 20.109(F19):Complete gamma-H2AX assay and prepare CometChip (Day3) (20:47, 25 September 2019)
- 20.109(F19):Complete CometChip experiment (Day5) (13:07, 26 September 2019)
- 20.109(F19):Image CometChip (Day6) (14:08, 1 October 2019)
- 20.109(F19):Practice statistical analysis methods and complete data analysis (Day7) (19:54, 2 October 2019)
- 20.109(F19):Complete in silico cloning of pdCas9 (Day1) (12:11, 8 October 2019)
- 20.109(F19): Assignments (16:14, 16 October 2019)
- 20.109(F19):Homework (17:37, 16 October 2019)
- 20.109(F19):Journal club presentation (Day4 and 6) (20:23, 22 October 2019)
- 20.109(F19): Data Summary (18:34, 23 October 2019)
- 20.109(F19):Confirm gRNA sequence in pgRNA (Day5) (17:02, 25 October 2019)
- 20.109(F19):Induce CRISPRi system (Day7) (18:41, 1 November 2019)
- 20.109(F19): Prep notes for M3 (18:44, 1 November 2019)
- Electronics bootcamp II: feedback systems (19:08, 1 November 2019)
- Assignment 8, Part 2: build a two-color microscope (16:20, 4 November 2019)
- 20.109(F19):Research proposal presentation (19:26, 5 November 2019)
- 20.109(F19):Measure fermentation products (Day8) (15:40, 6 November 2019)
- DAQ Ribbon Cable (23:19, 6 November 2019)
- 20.109(F19):Prepare for cellular thermal shift assay (Day1) (16:18, 7 November 2019)
- 20.109(F19):Communication (19:21, 7 November 2019)
- Assignment 8, Part 3: add flow control and test your device (22:11, 7 November 2019)
- 20.109(F19): Research article (23:37, 11 November 2019)
- Assignment 9, Part 1: Analyze two-color yeast images (16:08, 12 November 2019)
- Assignment 9 Overview: Analyzing yeast images (21:03, 14 November 2019)
- 20.109(F19):Incubate with ligand and apply heat treatment for protein denaturation (Day2) (19:04, 15 November 2019)
- 20.109(F19):Begin Western blot analysis (Day3) (20:06, 20 November 2019)
- Assignment 10, Part 1: Measuring the osmotic shock response of yeast (21:39, 21 November 2019)
- Assignment 11: Tell us about your lab visit (15:36, 2 December 2019)
- 20.109(F19): Mini-report (18:12, 3 December 2019)
- 20.109(F19):Class data (19:04, 3 December 2019)
- 20.109(F19):Data analysis and assignment preparation (Day5) (21:05, 3 December 2019)
- 20.109(F19): Fall 2019 schedule (19:19, 4 December 2019)
- Laser cutter safe operating procedure (19:03, 15 December 2019)
- 20.109(S20): Prep notes, orientation (16:40, 23 January 2020)
- Assignment 6, Part 1: build a two-color microscope (17:06, 23 January 2020)
- Electronics boot camp lab part 1 (17:21, 23 January 2020)
- Electronics boot camp I: passive circuits and transfer functions (17:22, 23 January 2020)
- Electronics written problems II (17:26, 23 January 2020)
- 20.109(S20):Module 1 (16:51, 30 January 2020)
- Assignment 1, Part 2: Optics bootcamp (06:40, 31 January 2020)
- 20.109(S20): Mini-presentation (16:15, 31 January 2020)
- 20.109(S20):Prepare clones for sequencing (Day3) (17:43, 3 February 2020)
- 20.109(S20):Identify clone to characterize (Day4) (17:43, 3 February 2020)
- 20.109(S20):Characterize clone by titration using flow cytometry (Day5) (17:43, 3 February 2020)
- 20.109(S20):Analyze titration curves (Day6) (17:44, 3 February 2020)
- 20.109(S20):Laboratory tour (14:55, 5 February 2020)
- 20.109(S20): Prep notes for M1 (20:35, 5 February 2020)
- 20.309:Course Information (16:37, 7 February 2020)
- 20.109(S20):Complete in silico cloning and induce TDP43 protein expression (Day1) (18:17, 7 February 2020)
- Assignment 1 Overview: Transillumination microscopy (17:05, 11 February 2020)
- 20.109(S20):Purify TDP43 protein (Day2) (19:52, 12 February 2020)
- Assignment 1, Part 4: Measuring magnification and bead size (04:16, 14 February 2020)
- Assignment 2: fluorescence microscopy (18:23, 14 February 2020)
