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  1. 20.109(F16):Complete in silico cloning (Day1)‏‎ (13,612 views)
  2. BE Classroom Computer Documentation‏‎ (13,565 views)
  3. 20.109(F20):M3D2‏‎ (13,531 views)
  4. Resource list: Optical trapping‏‎ (13,523 views)
  5. 20.109(F07): M13.1‏‎ (13,484 views)
  6. Resource list: DNA melting and PCR‏‎ (13,453 views)
  7. 20.109(S08):DNA engineering/DNA engineering by PCR (Day 1)‏‎ (13,426 views)
  8. Spring 2012:Leanna Morinishi Lit Search‏‎ (13,380 views)
  9. 20.109(F18):Complete in silico cloning (Day1)‏‎ (13,340 views)
  10. 20.109(S16):Prepare expression system (Day4)‏‎ (13,309 views)
  11. 20.109(F10)‏‎ (13,262 views)
  12. 20.109(S24):Spring 2024 schedule‏‎ (13,213 views)
  13. 20.109(F09):Lab tour‏‎ (13,212 views)
  14. 20.109(F11):People‏‎ (13,197 views)
  15. 20.109(S17):Purification of induced protein (Day2)‏‎ (13,189 views)
  16. 20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1)‏‎ (13,152 views)
  17. Methods‏‎ (13,132 views)
  18. ESTORM‏‎ (13,066 views)
  19. 20.109(S16):Cell preparation for DNA repair assays (Day5)‏‎ (13,061 views)
  20. Spring 2020 Assignment 7‏‎ (13,060 views)
  21. 20.109(S14)‏‎ (13,054 views)
  22. 20.109(F19):Incubate with ligand and apply heat treatment for protein denaturation (Day2)‏‎ (13,052 views)
  23. DNA Melter Improvements‏‎ (12,930 views)
  24. 20.109(S09): Transfection (Day5)‏‎ (12,901 views)
  25. Limits of Detection:Report Requirements‏‎ (12,877 views)
  26. 20.109(F17):Homework‏‎ (12,875 views)
  27. 20.109(S18):Induce DNA damage and apply drug treatments for RNA purification (Day2)‏‎ (12,839 views)
  28. 20.109(S16):Purify protein (Day6)‏‎ (12,813 views)
  29. 20.109(S18):Induce DNA damage and apply drug treatments for cell viability and identification of regulatory motifs in RNA-seq data (Day8)‏‎ (12,765 views)
  30. 20.109(F23):Fall 2023 schedule‏‎ (12,740 views)
  31. 20.109(F20):M3D3‏‎ (12,682 views)
  32. 20.109(F12):People‏‎ (12,659 views)
  33. Spring 11:Directed Evolution‏‎ (12,523 views)
  34. 20.109(S14): Choose system conditions and paper discussion (Day3)‏‎ (12,521 views)
  35. 20.109(F07): Fall 2007 schedule‏‎ (12,469 views)
  36. 20.109(F15):Lipofection (Day6)‏‎ (12,456 views)
  37. 20.109(F16):Seed cells for immuno-flourescence assay (Day5)‏‎ (12,454 views)
  38. 20.109(S17):In silico cloning and induction of protein expression (Day1)‏‎ (12,338 views)
  39. Electronics boot camp I: passive circuits and transfer functions‏‎ (12,321 views)
  40. Assignment 9, Part 3: Fitting your data‏‎ (12,313 views)
  41. Spring 11:eSTORM‏‎ (12,280 views)
  42. Spring 2012:LFM Report‏‎ (12,267 views)
  43. Shot noise and centroid finding‏‎ (12,261 views)
  44. Spring 2012: Jonathan Gootenberg Journal‏‎ (12,248 views)
  45. 20.109(S23):Class data‏‎ (12,239 views)
  46. 20.109(S13):People‏‎ (12,220 views)
  47. 20.109(S20):Characterize clone by titration using flow cytometry (Day4)‏‎ (12,210 views)
  48. 20.109(S18):Test protein activity using peptidyl-prolyl cis-trans isomerase assay (Day5)‏‎ (12,191 views)
  49. Optical Microscopy: Part 2 Report Outline‏‎ (12,135 views)
  50. 20.109(S18): Assignments‏‎ (12,093 views)
  51. 20.109(F18):Lab tour‏‎ (12,012 views)
  52. Relay Lens Example‏‎ (12,008 views)
  53. Spring 2012:LFM Proposal‏‎ (11,840 views)
  54. 20.109(F16):Complete immuno-fluorescence assay (Day6)‏‎ (11,802 views)
  55. Spring 2012:Leanna Morinishi Journal‏‎ (11,788 views)
  56. 20.109(S08):Site-directed mutagenesis (Day2)‏‎ (11,750 views)
  57. 20.109(F15):Growth of phage materials (Day1)‏‎ (11,738 views)
  58. 20.109(S17):Communication‏‎ (11,669 views)
  59. 20.109(S23):M2D6‏‎ (11,633 views)
  60. 20.109(F08)‏‎ (11,609 views)
  61. 20.109(S16)‏‎ (11,424 views)
  62. 20.109(S08):People‏‎ (11,413 views)
  63. Assignment 9, Part 2: Simulating DNA melting data and testing the model function‏‎ (11,409 views)
  64. Lab orientation‏‎ (11,408 views)
  65. 20.109(S14):Lab tour‏‎ (11,379 views)
  66. 20.109(F20):M2D2‏‎ (11,328 views)
  67. Python:Simulating DNA Melting‏‎ (11,282 views)
  68. Optical Microscopy Week 1: Build a brightfield microscope‏‎ (11,213 views)
  69. 20.109(S18):In silico cloning and confirmation digest of protein expression vector(Day1)‏‎ (11,208 views)
  70. 20.109(F18):Growth of phage-based active (cathode) material (Day1)‏‎ (11,190 views)
  71. 20.109(F16): M1 Data Summary‏‎ (11,175 views)
  72. 20.109(S20):Class data‏‎ (11,167 views)
  73. 20.109(F08):People‏‎ (11,143 views)
  74. 20.109(F20):Journal club presentation‏‎ (11,111 views)
  75. 20.109(S08): TA notes for module 1‏‎ (11,111 views)
  76. 20.109(F09): Mod 2 Day 3 Tools for system engineering‏‎ (11,104 views)
  77. 20.109(S20):Journal club presentation (Day4 and 6)‏‎ (11,050 views)
  78. 20.109(F16):Design gRNA for CRISPRi (Day2)‏‎ (11,049 views)
  79. Lab Manual:Introduction to electronics‏‎ (11,027 views)
  80. 20.109(F15):Purify protein (Day4)‏‎ (10,990 views)
  81. 20.109(F16):Induce CRISPRi system (Day7)‏‎ (10,978 views)
  82. 20.109(S18): Research article‏‎ (10,851 views)
  83. 20.109(S10)‏‎ (10,775 views)
  84. 20.109(S18):Evaluate protein purity and concentration (Day4)‏‎ (10,693 views)
  85. 20.109(F20):M1D4‏‎ (10,657 views)
  86. 20.109(S23):M2D1‏‎ (10,641 views)
  87. 20.109(F19):Complete gamma-H2AX assay and prepare CometChip (Day3)‏‎ (10,634 views)
  88. 20.109(F17):Complete biochemical experiment (Day5)‏‎ (10,609 views)
  89. 20.109(S16):Complete Western and prepare damaged DNA (Day3)‏‎ (10,584 views)
  90. 20.109(F16):Data analysis (Day7)‏‎ (10,575 views)
  91. 20.109(F17):Examine sub-nuclear foci abundance to measure DNA damage (Day6)‏‎ (10,488 views)
  92. A.Williams: Objectives and Literature Review‏‎ (10,483 views)
  93. 20.109(S14):DNA repair assays(Day6)‏‎ (10,480 views)
  94. 20.109(S14):People‏‎ (10,474 views)
  95. 20.109(F17):Develop experiment to optimize loading variables (Day2)‏‎ (10,437 views)
  96. 20.109(F20):M2D5‏‎ (10,404 views)
  97. 20.109(S20):Harvest candidate clones (Day2)‏‎ (10,342 views)
  98. 20.109(F09):People‏‎ (10,329 views)
  99. 20.109(S19):Test protein activity using peptidyl-prolyl cis-trans isomerase assay (Day5)‏‎ (10,292 views)
  100. 20.109(F18):Communication‏‎ (10,271 views)
  101. 20.109(F07): Module 2 oral presentations‏‎ (10,220 views)
  102. 20.109(S08): Spring 2008 schedule‏‎ (10,201 views)
  103. 20.109(S16): Protein engineering summary‏‎ (10,119 views)
  104. 20.109(F19):Load cells into CometChip and apply treatments (Day4)‏‎ (10,112 views)
  105. 20.109(S21):Module 3‏‎ (10,097 views)
  106. 20.109(S14):Microbiome summary‏‎ (10,077 views)
  107. 20.109:Module 4‏‎ (10,070 views)
  108. 20.109(S07):Bio-material engineering/Screening library‏‎ (10,028 views)
  109. 20.109(F20):M3D1‏‎ (9,979 views)
  110. 20.109(F20):M1D7‏‎ (9,971 views)
  111. Resource list: Suppliers‏‎ (9,951 views)
  112. 20.109(S16):Flow cytometry and paper discussion (Day7)‏‎ (9,926 views)
  113. Spring 2012:PSF lab‏‎ (9,916 views)
  114. 20.109(F15):Battery assembly and testing (Day5)‏‎ (9,893 views)
  115. 20.109(S22):M1D2‏‎ (9,893 views)
  116. Beam Expander Example‏‎ (9,881 views)
  117. 20.109(S23):Journal article presentation‏‎ (9,872 views)
  118. 20.109(S07): Start-up signal measurement‏‎ (9,858 views)
  119. Spring 2012:Leanna Morinishi Lab 2‏‎ (9,850 views)
  120. 20.109(S16):DNA repair assays (Day6)‏‎ (9,837 views)
  121. 20.109(F20):M2D6‏‎ (9,836 views)
  122. 20.109(F17):Prepare comet chips (Day1)‏‎ (9,829 views)
  123. 20.109(S20):Complete in silico cloning and induce TDP43 protein expression (Day1)‏‎ (9,738 views)
  124. 20.109(F19):Prepare for cellular thermal shift assay (Day1)‏‎ (9,729 views)
  125. 20.109(F18):Measure fermentation products (Day8)‏‎ (9,712 views)
  126. 20.109(F19):Image CometChip (Day6)‏‎ (9,579 views)
  127. 20.109(F09): Journal Club I‏‎ (9,572 views)
  128. 20.109(S21):M1D5‏‎ (9,570 views)
  129. Spring 2012:Vincent Lee Journal‏‎ (9,561 views)
  130. 20.109(S08):Student presentations (M2D8)‏‎ (9,555 views)
  131. 20.109(F19):Communication‏‎ (9,517 views)
  132. 20.109(S21):Communication‏‎ (9,506 views)
  133. Optical Microscopy: Part 3 Report Outline‏‎ (9,479 views)
  134. 20.109(F16):Generate gRNA plasmid (Day3)‏‎ (9,442 views)
  135. 20.109(F10): Fall 2010 schedule‏‎ (9,432 views)
  136. 20.109(S18):Laboratory tour‏‎ (9,420 views)
  137. 20.109(F19):Practice cell culture and begin sub-nuclear foci assay (Day1)‏‎ (9,376 views)
  138. 20.109(F08): Fall 2008 schedule‏‎ (9,369 views)
  139. 20.109(S22):Homework‏‎ (9,344 views)
  140. 20.109(F09): Journal Club II‏‎ (9,336 views)
  141. 20.109(S13):Design mutant (Day1)‏‎ (9,318 views)
  142. Results‏‎ (9,302 views)
  143. 20.109(S17):Evaluation of purified protein (Day3)‏‎ (9,301 views)
  144. 20.109(S18):Analyze small microarray data (Day3)‏‎ (9,301 views)
  145. 20.109(S22):M1D3‏‎ (9,299 views)
  146. 20.109(S16):Module 1‏‎ (9,276 views)
  147. 20.109(S13):Context-setting and primer design (Day1)‏‎ (9,251 views)
  148. 20.109(F20):Module 2‏‎ (9,242 views)
  149. Cellular microrheology‏‎ (9,239 views)
  150. 20.109(F22):Homework‏‎ (9,202 views)
  151. 20.109(F07)‏‎ (9,179 views)
  152. 20.109(F20):M1D3‏‎ (9,159 views)
  153. 20.109(F18):Design cell loading optimization experiment and discuss genomic instability experiment (Day2)‏‎ (9,149 views)
  154. 20.109(F20):M3D5‏‎ (9,147 views)
  155. 20.109(S14):Data analysis (Day7)‏‎ (9,104 views)
  156. 20.109(F20): Assignments‏‎ (9,093 views)
  157. 20.109(F17):Evaluate cell loading results (Day3)‏‎ (9,085 views)
  158. Recording, displaying and saving images in MATLAB‏‎ (9,074 views)
  159. 20.109(S17):Screen chemical library for FKBP12 binders (Day4)‏‎ (9,073 views)
  160. 20.109(F20):Communication‏‎ (9,034 views)
  161. 20.109(S17):Examine quantitative PCR results (Day9)‏‎ (9,029 views)
  162. 20.109(F12): Journal Club II‏‎ (9,020 views)
  163. DNA Melting II: Using the Matlab DNALockIn GUI‏‎ (9,016 views)
  164. 20.109(S21):M2D2‏‎ (9,007 views)
  165. Resource list: Electronics‏‎ (8,968 views)
  166. 20.109(F17):Communication‏‎ (8,951 views)
  167. 20.109(S18):Investigate RNA-seq data using public databases (Day5)‏‎ (8,916 views)
  168. 20.109(F16): Research article‏‎ (8,906 views)
  169. Williams: PSF and Deconvolution Lab 2‏‎ (8,882 views)
  170. Compound Microscope Example‏‎ (8,866 views)
  171. 20.109(S08): TA notes for module 2‏‎ (8,857 views)
  172. 20.109(F07):Module 1‏‎ (8,833 views)
  173. 20.109(F17):Test role of biochemical factors in genomic stability (Day4)‏‎ (8,806 views)
  174. 20.109(S22):Journal club presentation‏‎ (8,798 views)
  175. 20.109(F20):M1D1‏‎ (8,787 views)
  176. 20.109(F15):Cathode construction (Day3)‏‎ (8,781 views)
  177. 20.109(S08):Module 2‏‎ (8,780 views)
  178. 20.109(S19):Laboratory tour‏‎ (8,728 views)
  179. Spring 2011:CellFinder‏‎ (8,723 views)
  180. 20.109(S18): Prep notes for M1‏‎ (8,720 views)
  181. 20.109(S16): System engineering report‏‎ (8,707 views)
  182. 20.109(S20):Communication‏‎ (8,705 views)
  183. 20.109(S07): Western analysis‏‎ (8,694 views)
  184. 20.109(F08): Sign up for journal club‏‎ (8,670 views)
  185. 20.109(F22):M2D7‏‎ (8,663 views)
  186. 20.109(F17):Complete in silico cloning (Day1)‏‎ (8,648 views)
  187. 20.109(S09): siRNA design (Day1)‏‎ (8,627 views)
  188. 20.109(F19):Complete in silico cloning of pdCas9 (Day1)‏‎ (8,601 views)
  189. 20.109(F18):Practice tissue culture and prepare microwell array (Day1)‏‎ (8,599 views)
  190. 20.109(F19):Begin Western blot analysis (Day3)‏‎ (8,594 views)
  191. 20.109(F07): ECD experimental variations‏‎ (8,525 views)
  192. DNA Melting: DNA Sequences‏‎ (8,474 views)
  193. Spring 2012 Bioinstrumentation Project Lab‏‎ (8,425 views)
  194. Lab 1 Report -- Nathan S Lachenmyer‏‎ (8,409 views)
  195. 20.109(S11)‏‎ (8,406 views)
  196. 20.109(F07):Module 2‏‎ (8,393 views)
  197. 20.109(S13)‏‎ (8,371 views)
  198. 20.109(S13):PCR and paper discussion (Day3)‏‎ (8,350 views)
  199. 20.109(S21):Data Summary‏‎ (8,347 views)
  200. MRI lab: FPGA controller documentation‏‎ (8,313 views)
  201. 20.109(S16):Site-directed mutagenesis (Day3)‏‎ (8,266 views)
  202. 20.109(F18):Prepare and treat cells for genomic instability experiment (Day3)‏‎ (8,227 views)
  203. 20.109(S14):Complete Western and prepare damaged DNA (Day4)‏‎ (8,226 views)
  204. 20.109(F15):People‏‎ (8,218 views)
  205. 20.109(S20):Assess purity and concentration of TDP43 protein (Day3)‏‎ (8,188 views)
  206. 20.109(S20):Analyze RNA-seq data and select gene targets for quantitative PCR (qPCR) experiment (Day 6)‏‎ (8,188 views)
  207. 20.109(F17): Assignments‏‎ (8,184 views)
  208. Bryan Hernandez/20.109/Lab notebook/Module 1/Day 4‏‎ (8,124 views)
  209. 20.109(S08):Testing cell viability (Day3)‏‎ (8,122 views)
  210. 20.109(F16): Announcements‏‎ (8,080 views)
  211. 20.109(S13):DNA cloning (Day4)‏‎ (8,043 views)
  212. 20.109(F19):Complete CometChip experiment (Day5)‏‎ (8,016 views)
  213. 20.109(S18):Purify RNA and practice RNA-seq data analysis methods (Day3)‏‎ (7,997 views)
  214. Procedure: Particle tracking‏‎ (7,977 views)
  215. 20.109(F16):Measure DNA damage with comet chip (Day3)‏‎ (7,973 views)
  216. 20.109(F17):Visualize and analyze data for sub-nuclear foci assay (Day7)‏‎ (7,933 views)
  217. 20.109(F11): Fall 2011 schedule‏‎ (7,925 views)
  218. 20.109(S08):Induce protein expression (Day4)‏‎ (7,918 views)
  219. 20.109(F12): Assignments‏‎ (7,912 views)
  220. 20.109(S19):Communication‏‎ (7,895 views)
  221. 20.109(S08)‏‎ (7,848 views)
  222. Optical Microscopy Week 3: Experiments‏‎ (7,843 views)
  223. 20.109(F15):Wiki basics‏‎ (7,832 views)
  224. Resource list: Magnetic Resonance Imaging‏‎ (7,806 views)
  225. 20.109(F21):M2D3‏‎ (7,789 views)
  226. 20.109(F12): Journal Club I‏‎ (7,734 views)
  227. 20.109(S17):Prepare Western blot and induce DNA damage for quantitative PCR assay (Day2)‏‎ (7,734 views)
  228. Assignment 8, Part 1: convolution‏‎ (7,727 views)
  229. 20.109(S07): Lipofection‏‎ (7,720 views)
  230. 20.109(S10): Spring 2010 schedule‏‎ (7,712 views)
  231. 20.109(S18):Growth of phage-based active (cathode) material (Day1)‏‎ (7,701 views)
  232. 20.109(S20):Perform qPCR experiment and continue RNA-seq data analysis (Day7)‏‎ (7,683 views)
  233. 20.109(S14):Introduction to cell strains and plating (Day1)‏‎ (7,656 views)
  234. 20.109(S23):M2D7‏‎ (7,627 views)
  235. 20.109(S18):Purify protein for secondary assays(Day3)‏‎ (7,627 views)
  236. 20.109(F15):Assess protein function (Day7)‏‎ (7,622 views)
  237. 20.109(F09): Mod 2 Day 5 Assessing re-tuned system‏‎ (7,615 views)
  238. 20.109(S21):M2D5‏‎ (7,609 views)
  239. 20.109(S09):Prepare expression system (Day4)‏‎ (7,607 views)
  240. 20.109(F08):Lab tour‏‎ (7,576 views)
  241. 20.109(S07): cDNA synthesis and microarray‏‎ (7,563 views)
  242. 20.109(S19):Complete data analysis (Day9)‏‎ (7,520 views)
  243. 20.109(S17):Complete cell viability assay (Day9)‏‎ (7,491 views)
  244. 20.109(S21):M2D1‏‎ (7,489 views)
  245. 20.109(S16):People‏‎ (7,488 views)
  246. 20.109(F09): Mod 2 Day 1 Testing an engineered biological system‏‎ (7,474 views)
  247. 20.109(F21):Journal club presentation‏‎ (7,449 views)
  248. 20.109(S18):Analyze RNA-seq data and prepare for quantitative PCR experiment (Day 4)‏‎ (7,444 views)
  249. Final Project -- Nathan S Lachenmyer‏‎ (7,440 views)
  250. Introduction‏‎ (7,433 views)
  251. DNA Melting: Using the Matlab DNAMelter GUI‏‎ (7,417 views)
  252. 20.109(S14):Preparing cells for analysis (Day4)‏‎ (7,400 views)
  253. 20.109(S09)‏‎ (7,399 views)
  254. 20.109(S14):Begin Western protein analysis (Day2)‏‎ (7,393 views)
  255. 20.109(S09):Start-up protein engineering (Day1)‏‎ (7,382 views)
  256. 20.109(F21):M1D2‏‎ (7,356 views)
  257. 20.109(S14):Phylogenetic and primer analyses (Day7)‏‎ (7,348 views)
  258. 20.109(S14): TA notes for module 1‏‎ (7,347 views)
  259. 20.109(F15):Module 3‏‎ (7,341 views)
  260. MATLAB: Estimating viscoelastic spectrum using Mason's method‏‎ (7,317 views)
  261. 20.109(S18):Communication‏‎ (7,293 views)
  262. Weekly Updates‏‎ (7,291 views)
  263. 20.109(F19):Induce CRISPRi system (Day7)‏‎ (7,289 views)
  264. 20.109(F07): Module 1 oral presentations‏‎ (7,285 views)
  265. DNA Melting: Using the Basic DNAMelter GUI‏‎ (7,274 views)
  266. BEECH(F23):Build Knowledge‏‎ (7,266 views)
  267. 20.109(F15):Purify active materials (Day2)‏‎ (7,232 views)
  268. 20.109(F15):Characterize protein expression (Day6)‏‎ (7,219 views)
  269. 20.109(F16)::Measure fermentation products (Day8)‏‎ (7,217 views)
  270. 20.109(S19):Module 2‏‎ (7,200 views)
  271. 20.109(F20):Module 3‏‎ (7,199 views)
  272. 20.109(S19):Confirm ligand binding using differential scanning fluorimetry assay (Day6)‏‎ (7,185 views)
  273. Assignment 6, Part 1: build a two-color microscope‏‎ (7,171 views)
  274. DNA Melting: Processing DNA Melting Data‏‎ (7,135 views)
  275. Resource list: Optics‏‎ (7,116 views)
  276. 20.109(S08):Characterize protein expression (Day5)‏‎ (7,111 views)
  277. 20.109(S17):Complete Western blot and induce DNA damage for survival and quantitative PCR assays (Day2)‏‎ (7,034 views)
  278. 20.109(F17)::Measure fermentation products (Day8)‏‎ (7,019 views)
  279. 20.109(S17):Module 2‏‎ (7,009 views)
  280. 20.109(F16):Module 2‏‎ (6,998 views)
  281. 20.109(S14):16S PCR and paper discussion (Day3)‏‎ (6,985 views)
  282. 20.109(S08):Start-up protein engineering (Day1)‏‎ (6,977 views)
  283. 20.109(S16):Module 2‏‎ (6,947 views)
  284. 20.109(S07):Lab tour‏‎ (6,937 views)
  285. 20.109(S22):M1D7‏‎ (6,899 views)
  286. 20.109(S13):DNA sequencing and primer analysis (Day5)‏‎ (6,894 views)
  287. 20.109(F11): Journal Club II‏‎ (6,887 views)
  288. 20.109(S08):Module 1‏‎ (6,877 views)
  289. 20.109(F07):Module 3‏‎ (6,876 views)
  290. 20.109:Module 1‏‎ (6,876 views)
  291. 20.109(S18):Practice tissue culture techniques and prepare cells for RNA purification (Day1)‏‎ (6,852 views)
  292. 20.109(S13):Preparing cells for analysis (Day4)‏‎ (6,847 views)
  293. 20.109(S08):Preparing cells for analysis (Day4)‏‎ (6,844 views)
  294. 20.109(F10):People‏‎ (6,840 views)
  295. 20.109(S09):Induce protein and evaluate DNA (Day5)‏‎ (6,832 views)
  296. Assignment 7, Part 3: testing your instrument and measuring a DNA melting curve‏‎ (6,815 views)
  297. 20.109(F16):Asses DNA repair variability with comet chip (Day4)‏‎ (6,803 views)
  298. 20.109(F21):M2D1‏‎ (6,784 views)
  299. 20.109(S23):Homework‏‎ (6,772 views)
  300. 20.109(F07): siRNA design‏‎ (6,768 views)
  301. 20.109(S14):DNA cloning (Day4)‏‎ (6,727 views)
  302. 20.109(S09): Spring 2009 schedule‏‎ (6,699 views)
  303. 20.109(S21):Assignments‏‎ (6,675 views)
  304. 20.109(S11):Prepare DNA for cloning (Day3)‏‎ (6,673 views)
  305. 20.109(S14):DNA sequencing (Day5)‏‎ (6,666 views)
  306. Lab Manual:Optical Trapping‏‎ (6,665 views)
  307. 20.109(S16):Growth of phage materials (Day1)‏‎ (6,663 views)
  308. 20.109(F15):DNA engineering using PCR (Day1)‏‎ (6,647 views)
  309. Spring 2012:LFM‏‎ (6,630 views)
  310. 20.109(S16): Assignments‏‎ (6,599 views)
  311. 20.109(S17):Identify chemical structures common among FKBP12 binders‏‎ (6,586 views)
  312. 20.109(S20):Module 3‏‎ (6,571 views)
  313. 20.109(F21):M1D3‏‎ (6,549 views)
  314. 20.109(S10):Prepare RNA by IVT (Day3)‏‎ (6,542 views)
  315. 20.109(S21):M2D4‏‎ (6,540 views)
  316. 20.109 (F07): Phage by design‏‎ (6,540 views)
  317. DNA Melting: Using the LockIn DNAMelter GUI‏‎ (6,470 views)
  318. 20.109(F10): Journal Club II‏‎ (6,459 views)
  319. 20.109(F12): Mod 2 Day 3 Tools for system engineering‏‎ (6,408 views)
  320. 20.109(F12): WF Blue pre-proposal‏‎ (6,398 views)
  321. 20.109(F17):Lab tour‏‎ (6,388 views)
  322. 20.109(F07): M13.1 and MDS titers‏‎ (6,386 views)
  323. 20.109(S17):Scan slides to identify FKBP12 binders (Day5)‏‎ (6,374 views)
  324. Optical Microscopy Data Sheets‏‎ (6,369 views)
  325. 20.109(S19): Assignments‏‎ (6,349 views)
  326. 20.109(S22):M2D5‏‎ (6,347 views)
  327. Resource list: Microscopy‏‎ (6,335 views)
  328. 20.109(S10):Prepare expression system (Day4)‏‎ (6,326 views)
  329. 20.109(S07): Yeast transformation‏‎ (6,262 views)
  330. Matlab:Division operators‏‎ (6,261 views)
  331. 20.109(S16):Induce protein expression (Day5)‏‎ (6,251 views)
  332. 20.109(S14):Cell preparation for DNA repair assays (Day5)‏‎ (6,230 views)
  333. 20.109(S21):Module 2‏‎ (6,224 views)
  334. 20.109(F15): DNA engineering presentation‏‎ (6,220 views)
  335. Final Project Proposal Williams‏‎ (6,207 views)
  336. 20.109(F20):M2D7‏‎ (6,201 views)
  337. 20.109(F15):Lab tour‏‎ (6,184 views)
  338. 20.109(F18):Module 1‏‎ (6,169 views)
  339. 20.109(F12):Lab tour‏‎ (6,167 views)
  340. 20.109(S20):Enrich candidate clones using fluorescence-activated cell sorter (FACS) (Day1)‏‎ (6,167 views)
  341. 20.109(F08): Mod 2 Day 4 SDS-PAGE, screen for phenotypes‏‎ (6,166 views)
  342. 20.109(S17): Announcements‏‎ (6,150 views)
  343. 20.109(S08):Prepare expression system (Day3)‏‎ (6,147 views)
  344. 20.109(F11): Mod 3 Day 4 Solar cell assembly‏‎ (6,110 views)
  345. 20.109(F21):M2D6‏‎ (6,092 views)
  346. 20.109(S11):Complete DNA design (Day2)‏‎ (6,091 views)
  347. 20.109(F07): Luciferase assays and RNA prep‏‎ (6,085 views)
  348. 20.109(S17):Module 1‏‎ (6,069 views)
  349. 20.109(S07): Colony PCR‏‎ (6,068 views)
  350. 20.109(F15):Examine candidate clones & tissue culture (Day5)‏‎ (6,066 views)
  351. 20.109(F12):Module 2‏‎ (6,065 views)
  352. 20.109(F15): TA's notes for module 3‏‎ (6,064 views)
  353. 20.109(S13):Phylogenetic analysis (Day7)‏‎ (6,035 views)
  354. 20.109(S14): TA notes for module 2‏‎ (6,013 views)
  355. 20.109(F22):M2D2‏‎ (5,985 views)
  356. 20.109(S07): Bio-material engineering/Sequence analysis‏‎ (5,977 views)
  357. 20.109(S21):M2D3‏‎ (5,973 views)
  358. 20.109(S19):Purify RNA and practice RNA-seq data analysis methods (Day3)‏‎ (5,969 views)
  359. 20.109(S19):Module 1‏‎ (5,969 views)
  360. Spring 2012:Vincent Lee Lab 1‏‎ (5,952 views)
  361. 20.109(S21):Announcements‏‎ (5,937 views)
  362. 20.109(S17): Assignments‏‎ (5,926 views)
  363. 20.109(F08): Mod 2 Day 6 Journal Club I‏‎ (5,916 views)
  364. 20.109(F15): Assignments‏‎ (5,914 views)
  365. 20.109(F21):M2D5‏‎ (5,907 views)
  366. 20.109(S11): TA notes for module 2‏‎ (5,898 views)
  367. 20.109(S13): Assignments‏‎ (5,889 views)
  368. 20.109(S19):Design cell viability experiment and complete RNA-seq data analysis (Day6)‏‎ (5,883 views)
  369. 20.109(F11): Journal Club I‏‎ (5,861 views)
  370. 20.109(F15): TA notes for module 2‏‎ (5,856 views)
  371. 20.109(F22):M1D4‏‎ (5,810 views)
  372. 20.109(S17):Growth of phage materials (Day1)‏‎ (5,799 views)
  373. 20.109(S19): Data Summary‏‎ (5,785 views)
  374. 20.109(F16):Research proposal presentation‏‎ (5,771 views)
  375. 20.109(S20): Assignments‏‎ (5,763 views)
  376. 20.109(F07): ECD assembly‏‎ (5,757 views)
  377. 20.109(F08): Mod 3 Day 3 Journal Club II‏‎ (5,756 views)
  378. 20.309:Learning outcomes‏‎ (5,753 views)
  379. Assignment 6, Part 2: electronics written problems‏‎ (5,728 views)
  380. 20.109(S24):Class data‏‎ (5,703 views)
  381. 20.109(F17):Module 1‏‎ (5,701 views)
  382. 20.109(S16):Data analysis (Day9)‏‎ (5,695 views)
  383. 20.109(S24):M2D1‏‎ (5,672 views)
  384. Technical Writing‏‎ (5,625 views)
  385. 20.109(F17): M1 Data Summary‏‎ (5,614 views)
  386. 20.109(S22):M1D8‏‎ (5,605 views)
  387. Assignment 6, Part 1: Pre-lab questions‏‎ (5,601 views)
  388. Spring 2012: Jonathan Gootenberg Lab 2‏‎ (5,588 views)
  389. 20.109(F18):Complete genomic instability experiment and load cells for sub-nuclear foci assay (Day4)‏‎ (5,578 views)
  390. Equipment on loan‏‎ (5,573 views)
  391. Super resolution microscopy‏‎ (5,565 views)
  392. 20.345:Key Factors Table‏‎ (5,535 views)
  393. 20.109(F15):TEM (Day4)‏‎ (5,531 views)
  394. 20.109(S20): Announcements‏‎ (5,514 views)
  395. Spring 2012: Vincent Lee‏‎ (5,511 views)
  396. 20.309:DAQ System‏‎ (5,497 views)
  397. 20.109(S09): cDNA synthesis and microarray (Day7)‏‎ (5,489 views)
  398. 20.109(F19):Confirm gRNA sequence in pgRNA (Day5)‏‎ (5,488 views)
  399. 20.109(S14):Microbial DNA extraction (Day1)‏‎ (5,469 views)
  400. 20.109:Module 2‏‎ (5,468 views)
  401. 20.109(F07): Transfection‏‎ (5,459 views)
  402. Procedure: Peptide-DNA Tethering Assay‏‎ (5,454 views)
  403. Matlab:Matlab Fundamentals‏‎ (5,448 views)
  404. 20.109(F07): Assignments‏‎ (5,444 views)
  405. 20.109(F10):Module 2‏‎ (5,442 views)
  406. 20.109(S08):Lab tour‏‎ (5,441 views)
  407. 20.109(S22):M2D4‏‎ (5,422 views)
  408. 20.109(S19): Research article‏‎ (5,397 views)
  409. 20.109(F16):Module 3‏‎ (5,395 views)
  410. 20.109(S18): M1 Data Summary‏‎ (5,381 views)
  411. 20.109(S21):Laboratory tour‏‎ (5,356 views)
  412. 20.109(F12):Guidelines for oral presentations‏‎ (5,337 views)
  413. 20.109(S16):OWW basics‏‎ (5,331 views)
  414. 20.109(S23):M2D5‏‎ (5,300 views)
  415. 20.109(S18):Research proposal presentation‏‎ (5,295 views)
  416. 20.109(S16):Lab tour‏‎ (5,284 views)
  417. Optical Microscopy: Part 1 Report Outline‏‎ (5,256 views)
  418. Status Report Interrogatories (Spring 2012)‏‎ (5,241 views)
  419. 20.109(S20): Research article‏‎ (5,233 views)
  420. 20.109(F12): Mod 3 Day 1 Growth of phage materials‏‎ (5,215 views)
  421. 20.109(F19):Lab tour‏‎ (5,198 views)
  422. 20.109(F16):Growth of phage materials (Day1)‏‎ (5,168 views)
  423. 20.109(F07): Growth of phage materials‏‎ (5,161 views)
  424. 20.309: Exam 1 Topics‏‎ (5,141 views)
  425. 20.109(S17): Research article‏‎ (5,129 views)
  426. 20.109(F19):Module 1‏‎ (5,110 views)
  427. 20.109(F08): Mod 2 Day 5 Probe western, isolate RNA‏‎ (5,039 views)
  428. 20.109(F15): Protein engineering report‏‎ (5,034 views)
  429. 20.109(F23):Class data‏‎ (5,033 views)
  430. 20.109(S22):M1D5‏‎ (5,025 views)
  431. 20.109(F21):M1D4‏‎ (5,023 views)
  432. 20.109(F20):M2D4‏‎ (5,013 views)
  433. 20.109(F23):M2D5‏‎ (4,990 views)
  434. 20.109(S08):Transcript-level analysis (Day5)‏‎ (4,987 views)
  435. Spring 2012: Jonathan Gootenberg‏‎ (4,979 views)
  436. 20.109(F21):Research article‏‎ (4,978 views)
  437. 20.109(F15):Student registration and questionnaire‏‎ (4,959 views)
  438. 20.109(S14):Module 2‏‎ (4,956 views)
  439. 20.109(S22):M1D4‏‎ (4,952 views)
  440. 20.109(F11): Mod 2 Day 3 Tools for system engineering‏‎ (4,940 views)
  441. 20.109(F07): Genome engineering assessment‏‎ (4,932 views)
  442. 20.109(S17): M1 Data Summary‏‎ (4,930 views)
  443. 20.109(F15):Clean and cut DNA (Day2)‏‎ (4,924 views)
  444. Electronics written problems II‏‎ (4,918 views)
  445. 20.109(S10):Data analysis (Day8)‏‎ (4,883 views)
  446. 20.109(S20):Scan SMM slides to identify binders of TDP43 protein (Day5)‏‎ (4,879 views)
  447. 20.109(F07): Phage by design, pt2‏‎ (4,878 views)
  448. 20.109(F20): Announcements‏‎ (4,872 views)
  449. 20.109(S14):Diagnostic primer design (Day2)‏‎ (4,871 views)
  450. 20.109(S18):Construct cathode with phage-based active material (Day3)‏‎ (4,870 views)
  451. 20.109(F18):Confirm gRNA sequence (Day5)‏‎ (4,868 views)
  452. 20.109(F17):Module 3‏‎ (4,858 views)
  453. Aligning the optical trap‏‎ (4,851 views)
  454. 20.109(S09): Luciferase assays and RNA prep (Day6)‏‎ (4,847 views)
  455. 20.109(S17):Module 3‏‎ (4,843 views)
  456. Spring 2020 Assignment 10‏‎ (4,824 views)
  457. 20.109(S07): Calcium signaling in vivo‏‎ (4,811 views)
  458. 20.109(S08):Module 3‏‎ (4,768 views)
  459. 20.109(S16): TA notes for module 1‏‎ (4,750 views)
  460. 20.309 development to-do list‏‎ (4,750 views)
  461. 20.109(F21):Assignments‏‎ (4,745 views)
  462. 20.109(S22):M2D7‏‎ (4,738 views)
  463. Assignment 5: Spring 2020‏‎ (4,722 views)
  464. 20.109(S19):Analyze RNA-seq data and prepare for quantitative PCR experiment (Day 4)‏‎ (4,720 views)
  465. 20.109(F17):Confirm gRNA sequence (Day5)‏‎ (4,706 views)
  466. Review of probability concepts‏‎ (4,702 views)
  467. 20.109(S07): Measuring calcium in vivo‏‎ (4,693 views)
  468. QRT-PCR‏‎ (4,687 views)
  469. 20.109(S22):M2D6‏‎ (4,682 views)
  470. 20.109(F21):M1D6‏‎ (4,677 views)
  471. 20.109(S17):Assess cell survival and harvest RNA for quantitative PCR assay (Day3)‏‎ (4,670 views)
  472. 20.109(F15):DNA ligation & transformation (Day4)‏‎ (4,642 views)
  473. 20.109(F17): Announcements‏‎ (4,637 views)
  474. 20.109(F19): Data Summary‏‎ (4,631 views)
  475. Spring 2011:3D PSF lab:Kristin‏‎ (4,592 views)
  476. 20.109(F17):Growth of phage materials (Day1)‏‎ (4,590 views)
  477. 20.109(S20):Examine qPCR results and complete data analysis (Day8)‏‎ (4,584 views)
  478. 20.109(F18): Data Summary‏‎ (4,582 views)
  479. 20.109(F07): Journal article discussion‏‎ (4,575 views)
  480. Spring 11:CellPrinter‏‎ (4,571 views)
  481. 20.109(S16):Purify active materials (Day2)‏‎ (4,570 views)
  482. 20.109(S24):Journal article presentation‏‎ (4,553 views)
  483. 20.109(S17):Lab tour‏‎ (4,547 views)
  484. 20.109(F17):Module 2‏‎ (4,520 views)
  485. 20.109(S19):Perform quantitative PCR experiment and explore additional RNA-seq datasets (Day5)‏‎ (4,507 views)
  486. 20.109(S10):Purify RNA and run affinity column (Day4)‏‎ (4,504 views)
  487. 20.109(S10):Start-up protein engineering (Day1)‏‎ (4,496 views)
  488. 20.109(S23):M2D3‏‎ (4,495 views)
  489. 20.109(F09): Mod 2 Day 6 Readouts of DNA, Protein‏‎ (4,494 views)
  490. 20.109(F18):Complete sub-nuclear foci assay (Day6)‏‎ (4,492 views)
  491. 20.109(F07):Lab tour‏‎ (4,490 views)
  492. Spring09:Todo list‏‎ (4,489 views)
  493. 20.109(S13):DNA extraction (Day2)‏‎ (4,466 views)
  494. 20.109(F23):Homework‏‎ (4,459 views)
  495. 20.109(F09): Mod 3 Day 2 Phage nanowires‏‎ (4,456 views)
  496. 20.109(F22):M1D2‏‎ (4,453 views)
  497. 20.109(F19):Complete antibody staining for Western blot analysis (Day4)‏‎ (4,452 views)
  498. 20.109(S22):Laboratory tour‏‎ (4,451 views)
  499. 20.109(F21):FYI‏‎ (4,444 views)
  500. 20.109(S13):Bacterial amplification of DNA (Day3)‏‎ (4,438 views)

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