20.109(S10): Spring 2010 schedule

MODULE	DAY	DATE	LECTURER	TA	LAB	note
		Tue Feb 2	ANS		Orientation	* Introductory Lecture (PDF) * Intro Pre-Lab, T/R
1	1	Thur Feb 4	JN	CB]	Amplify aptamer-encoding DNA	* M1D1 Lecture * M1D1 Pre-Lab
1	2	Tue Feb 9	JN	CB]	Purify aptamer-encoding DNA	* M1D2 Lecture * M1D2 Pre-Lab
1	3	Thur Feb 11	JN	CB]	Prepare RNA by IVT	* M1D3 Lecture * M1D3 Pre-Lab
		President's Day Feb 15				No lab Feb 16 or Feb 17
1	4	Thur Feb 18	JN	CB]	Purify RNA and run affinity column	* M1D4 Lecture * M1D4 Pre-Lab
1	5	Tue Feb 23	JN	CB]	RNA to DNA by RT-PCR	* M1D5 Lecture * M1D5 Pre-Lab
1	6	Thur Feb 25	JN	CB]	Post-selection IVT and journal club	* M1D6 Lecture * M1D6 Pre-Lab
1	7	Tue Mar 2	JN	CB]	Aptamer binding assay	* M1D7 Lecture * M1D7 Pre-Lab
1	8	Thur Mar 4	JN	CB]	Journal club	* M1D8 Lecture
2	1	Tue Mar 9	AJ	XY]	Start-up protein engineering	Module 1 Draft Due * M2D1 Lecture * M2D1 Pre-Lab
2	2	Thur Mar 11	AJ	XY]	Site-directed mutagenesis	* M2D2 Lecture * M2D2 Pre-Lab
2	3	Tue Mar 16	AJ	XY]	Bacterial amplification of DNA	* M2D3 Lecture * M2D3 Pre-Lab
2	4	Thur Mar 18	AJ	XY]	Prepare expression system	* M2D4 Lecture * M2D4 Pre-Lab
		Spring Break Mar 22-26				No lab all week
2	5	Tue Mar 30	AJ	XY]	Induce protein and evaluate DNA	* M2D5 Lecture * M2D5 Pre-Lab
2	6	Thur Apr 1	AJ	XY]	Characterize protein expression	Module 1 Final Report Due * M2D6 Lecture * M2D6 Pre-Lab
2	7	Tue Apr 6	AJ	XY]	Assay protein behavior	* M2D7 Lecture
2	8	Thur Apr 8	AJ	XY]	Data analysis	* M2D8 Lecture
3	1	Tues Apr 13	ANS]	NC]	Start-up biomaterials engineering	Module 2 Draft Due * M3D1 Lecture * M3D1 Pre-Lab
3	2	Thur Apr 15	ANS]	NC]	Initiate cell culture	* M3D2 Lecture
		Patriot's Day April 19-20				No lab Apr 20 or Apr 21
3	3	Thur Apr 22	ANS]	NC]	Testing cell viability	* M3D3 Lecture * M3D3 Pre-Lab
3	4	Tue Apr 27	ANS]	NC]	Preparing cells for analysis	* M3D4 Lecture * M3D4 Pre-Lab
3	5	Thur Apr 29	ANS]	NC]	Transcript-level analysis	* M3D5 Lecture * M3D5 Pre-Lab
3	6	Tue May 4	ANS]	NC]	Protein-level analysis	Module 2 Final Report Due * M3D6 Lecture * M3D6 Pre-Lab
3	7	Thur May 6	ANS]	NC]	Wrap-up analysis	Module 3 Report Due *Atissa's Lecture * M3D7 Pre-Lab
3	8	Tue May 11	ANS]	NC]	Student presentations
		Thur May 13			No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD