20.109(S23):Class data
Contents
Module 1: Drug discovery
SDS-PAGE
T/R
Red Team: Media:TR Red S23M1D3.jpg
Orange Team: Media:TR Orange S23M1D3.jpg
Yellow Team: Media:TR Yellow S23M1D3.jpg
Green Team: Media:TR Green S23M1D3.jpg
Blue Team: Media:TR Blue S23M1D3.jpg
Pink Team: Media:TR Pink S23M1D3.jpg
Purple Team: Media:TR Purple S23M1D3.jpg
W/F
Dropbox Link : https://www.dropbox.com/sh/1p3sfs2487pkwr0/AAAAA-W0W6vPcXcfcp2nV2TPa?dl=0
BCA
W/F
Western Blot
W/F
Dropbox Link: https://www.dropbox.com/scl/fo/xlv4yz02oq2pfhka7mcmt/h?dl=0&rlkey=x1g0jqms054n0ycy3s2atbzrf
SMM
Module 2: Protein engineering
Mutagenesis
T/R
| Team | Mutation (eg. S301L) | Forward primer | Reverse primer |
| TR red | Y276C | TGGCAGATGTgTATTAACACTG | AGCGTCACTGAACTTTTTC |
| TR orange | Y276C | TTGGCAGATGtgtATTAACACTG | GCGTCACTGAACTTTTTC |
| TR yellow | Y276C | GGCAGATGTAcATTAACACTG | AAGCGTCACTGAACTTTTTC |
| TR green | Y352C | GATTGATTGGtgtGCTGATATTATTG | ATCTTTCTCCCTGTCGAC |
| TR blue | Y276C | TGGCAGATGTgTATTAACACTG | AGCGTCACTGAACTTTTTC |
| TR pink | D354C | TTGGTATGCTtgtATTATTGGTACTGG | TCAATCATCTTTCTCCCTG |
| TR purple | Y276C | TGGCAGATGTgTATTAACACTG | AGCGTCACTGAACTTTTTC |
T/R Sequencing [here]
W/F
| Team | Mutation (eg. S301L) | Forward primer | Reverse primer |
| WF red | V281C | TAACACTGCAtgTGCAGTTTCCCTTC | ATATACATCTGCCAAGCG |
| WF orange | I349C | GAGAAAGATGtgTGATTGGTATGCTGATATTATTG | CCTGTCGACCTTTTTTTAG |
| WF yellow | R405C | TTTCGTTTTGtgtGAAGTGTACTTCCG | CCGTCCAAAAACCCAATTAAACâ |
| WF green | D271C | AAAGTTCAGTTGCGCTTGGCAGATGTATATTAACACTGCAGTTGC (AAAGTTCAGTtgcGCTTGGCAGATG) | TTCAATCTTGGATTACTACCTGTAGTTTCTCCGGTGTAAGGTGGA (TTCAATCTTGGATTACTACCTG) |
| WF blue | Y408C | ||
| WF pink | D400C | ATTGGGTTTTTGtgCGGTTTCGTTTTG (TGGGTTTTTGtgcGGTTTCGTTTTG) | TAAACCTGTGTATGTACCGATAATCAACCACC (ATTAAACCTGTGTATGTACC) |
| WF purple | D350C | AAAGATGATTtgtTGGTATGCTGATATTATTGGTAC | CTCCCTGTCGACCTTTTTTTAG |
WF Sequencing here
Immunofluorescence Data
Pilot Immunofluorescence Data [[1]]
ICP-OES data
T/R
Raw ICP-OES data [here]
| Team | Fe (ppm)/ Wavelength | Cd (ppm)/ Wavelength | O.D. |
| TR red | 2.62 ppm / 240.489 nm | 7.58 ppm / 361.287 nm | 1.889 |
| TR orange | 4.29 ppm / 261.382 nm | 11.16 ppm / 326.105 nm | 1.566 |
| TR yellow | 3.70 ppm / 261.382 nm | 11.35 ppm / 346.766 nm | 1.529 |
| TR green | 4.58 ppm / 238.863 nm | 11.50 ppm / 479.991 nm | 1.495 |
| TR blue | 4.66 ppm / 259.837 nm | 11.52 ppm /479.999 nm | 1.546 |
| TR pink | 4.03ppm / 238.863nm | 10.52ppm / 232.929nm | 1.581 |
| TR purple | 4.19 ppm / 238.204 nm | 10.90 ppm / 223.986 nm | 1.458 |
| TR WT | 1 | ||
| TR Fet4 | 1.28 |
W/F
Raw ICP-OES data [here]
| Team | Fe (ppm)/ Wavelength | Cd (ppm)/ Wavelength | O.D. |
| WF red | 4.09 / 238.863 | 10.99 /213.439 | 1.486 |
| WF orange | 3.73 / 238.204 | 11.63 / 226.502 | 1.427 |
| WF yellow | 4.22 / 238.204 | 10.47 / 361.287 | 1.421 |
| WF green | |||
| WF blue | 5.17 / 238.863 | 12.58 / 214.439 | 1.322 |
| WF pink | 4.78 / 261.382 | 10.89 / 361.287 | 1.486 |
| WF purple | 4.52 / 261.382 | 11.52 / 361.287 | 1.205 |
| WF WT | 1.18 | ||
| WF Fet4 | 1.4 |
Cytotoxicity data
[TR section] [WF section]
1) Untransformed No Cadmium OD - 1.5 2) Untransformed Cadmium OD - 1.4 3) FET4 No Cadmium OD - 1.44 4) FET4 Cadmium OD - 1.44 5) Purple No Cadmium 1.733
6) Purple Cadmium 1.778
