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  • 20.109(S10):Journal club (Day8)
    ...at 1:30 pm sharp in room 16-336. (Day 6 presentations will begin after lab work is finished, b/w 1:30 and 2 pm.)
    7 KB (1,098 words) - 19:49, 28 July 2015
  • 20.109(S10): Assignments
    ...ter in lab, but <font color = CC0000>'''you must submit individual written work (for both daily homeworks and major assignments) and give individual journa ...</font color> We strongly recommend that you plan ahead and space out your work when possible.
    5 KB (682 words) - 19:49, 28 July 2015
  • 20.109(S10):Start-up protein engineering (Day1)
    ...s wasting a month of your time repeating experiments already proven not to work or reinventing the wheel. ...As you work, you can ask yourself why these stretches of the protein might work the way that they do, and how they might be changed.
    23 KB (3,840 words) - 19:49, 28 July 2015
  • 20.109(S10):Site-directed mutagenesis (Day2)
    ...al of information in order to design mutagenized inverse pericams – nice work! Today you will put your designs into practice. ...reaction, to ensure that all the reagents are working properly. You should work quickly but carefully, and keep your tube in a chilled container at all tim
    13 KB (2,131 words) - 19:49, 28 July 2015
  • 20.109(S10):Bacterial amplification of DNA (Day3)
    While the gel runs, you can label the tubes you will need later, work on your notebooks, start the FNT assignment, etc. ...ke your teaching faculty very happy if you contribute to their preparatory work. Please label 2 large glass test tubes with your team color and sample name
    11 KB (1,861 words) - 19:49, 28 July 2015
  • 20.109(S10):Prepare expression system (Day4)
    #Incubate on ice for 1 hour. (You might work on parts 2, 4, and 5 of today's protocols now, as well as assemble the mate ...motivate the audience to keep reading! How? Reveal the significance of the work through connections to both prior scientific accomplishments and future app
    22 KB (3,701 words) - 19:49, 28 July 2015
  • 20.109(S10):Induce protein and evaluate DNA (Day5)
    As evidenced by Nagai’s work, wild-type inverse pericam is not toxic to BL21(DE3) cells. Although it is ...our cells with IPTG, you will let the resultant protein factories do their work for 2-3 hours. During this time, you will evaluate the DNA from your two X#
    17 KB (2,849 words) - 19:49, 28 July 2015
  • 20.109(S10):Assay protein behavior (Day7)
    Only 2-3 groups at a time will work in lab today. Last time you should have signed up to arrive at 1:05, 2:20 o #Now work your way from reservoirs #2 to #12 (highest calcium concentration), and fro
    7 KB (1,187 words) - 19:49, 28 July 2015
  • 20.109(S10):Data analysis (Day8)
    ...got last time. Begin by analyzing the wild-type protein as a check on your work (your curve should resemble Nagai's Figure 3L), then move on to your mutant ...t be discouraged if your wild-type values do not exactly match Nagais work, or if there is variation between Parts 1, 2, and 3.
    15 KB (2,419 words) - 19:49, 28 July 2015
  • 20.109(S10):Guidelines for oral presentations
    ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.
    5 KB (788 words) - 19:49, 28 July 2015
  • 20.109(S10):Guidelines for writing up your research
    ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed. ...motivate the audience to keep reading! How? Reveal the significance of the work through connections to both prior scientific accomplishments and future app
    20 KB (3,083 words) - 19:49, 28 July 2015
  • 20.109(S10):Start-up biomaterials engineering (Day1)
    ... of the surrounding environment on cell phenotype. In particular, you will work with primary chondrocytes and/or mesenchymal stem cells in 3D gel culture. ...er time. We will also have some mesenchymal stem cells for a few groups to work with, and investigate conditions that promote chondrogenesis. Please see th
    15 KB (2,438 words) - 19:49, 28 July 2015
  • 20.109(S10):Initiate cell culture (Day2)
    ...eir knee joints is more similar to that of humans. In this module, we will work with an ''in vitro'' culture model of cartilage-forming cells. ... time however you find useful (FNT assignment, notebook prep, or unrelated work).
    12 KB (1,957 words) - 19:49, 28 July 2015
  • 20.109(S10):Testing cell viability (Day3)
    ...r labmates time.''' Reading the protocol in advance will help you work more quickly, and is strongly recommended. ...performing the viability assay, and/or during incubation steps, you should work on Part 3 of today's protocol.
    16 KB (2,677 words) - 22:02, 28 August 2016
  • 20.109(S10):Preparing cells for analysis (Day4)
    ...did when washing your freshly synthesized beads. A 10 mL pipet size should work well for most beads, while for very delicate beads, you should use a 2 mL s ...eting your cells, clean your microfuge. You can finish setting up your RNA work area while the cells spin down. See [[20.109%28S10%29:Prepare_RNA_by_IVT_%2
    15 KB (2,534 words) - 19:49, 28 July 2015
  • 20.109(S11):Guidelines for oral presentations
    ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.
    5 KB (826 words) - 21:05, 28 July 2015
  • 20.109(S11):Guidelines for working in the tissue culture facility
    ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.
    7 KB (1,163 words) - 21:05, 28 July 2015
  • 20.109(S11):Guidelines for writing up your research
    ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed. ...motivate the audience to keep reading! How? Reveal the significance of the work through connections to both prior scientific accomplishments and interestin
    20 KB (3,142 words) - 21:05, 28 July 2015
  • 20.109(S11):Ligate DNA and transform bacteria (Day4)
    ...shown in the figure below). A 5' phosphate is required for the ligation to work well; thus, the oligos we ordered from IDT were prepared with this modifica ...Besides the options listed in Part 1, Step 2 below, one other approach may work well. First, prepare for your &beta;-gal assay but don't do lyse the cells
    10 KB (1,629 words) - 21:05, 28 July 2015
  • 20.109(S11):Guidelines for editing OpenWetWare
    ==Sign your work==
    3 KB (504 words) - 21:05, 28 July 2015
  • 20.109(S11): Assignments
    ...ter in lab, but <font color = CC0000>'''you must submit individual written work (for both daily homeworks and major assignments) and give individual journa ...</font color> We strongly recommend that you plan ahead and space out your work when possible.
    5 KB (753 words) - 21:05, 28 July 2015
  • 20.109(S11): TA notes for module 3
    #None: all work today is computer work. Get passing familiarity with the faculty-selected journal articles. #150 mM NaCl in autoclaved water--needs to be sterile filtered
    10 KB (1,512 words) - 21:05, 28 July 2015
  • 20.109(S11):Module 3 oral presentations
    * needed resources to complete the work
    5 KB (867 words) - 21:05, 28 July 2015
  • 20.109(S11): RNA engineering report
    ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    6 KB (1,012 words) - 21:05, 28 July 2015
  • 20.109(S11): RNA computational analysis
    ...f 20.109, we do not expect you to know the details of how these algorithms work.
    12 KB (1,829 words) - 21:05, 28 July 2015
  • 20.109(S11):Start-up biomaterials engineering (Day1)
    ...o you should decide who will seed at 1:10 and who will seed at 1:2. As you work, try to put into practice what you learned about sterile technique in the d ... of the surrounding environment on cell phenotype. In particular, you will work with primary chondrocytes and/or mesenchymal stem cells in 3D gel culture.
    16 KB (2,581 words) - 21:05, 28 July 2015
  • 20.109(F12): System engineering research article
    *conclusions you can draw from your work, including any uncertainties *limitations of your work, e.g. what kinds of experiments/controls/samples would have been great to i
    6 KB (919 words) - 13:41, 29 July 2015
  • 20.109(F12)
    ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of ... all enjoyed with this module and a HUGE thanks to Jackie for all her hard work to make this experiment run so smoothly.
    4 KB (608 words) - 13:41, 29 July 2015
  • 20.109(F12): Assignments
    #'''Late work is penalized 1/3 of a letter grade each day late.''' #Only work turned in on time is guaranteed timely or extensive feedback.
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  • 20.109(F12): Fall 2012 schedule
    | You must choose a Journal Club article to work on during this module. Please sign up for your article on the wiki before y
    9 KB (1,386 words) - 13:41, 29 July 2015
  • 20.109(F12): test pages 2
    #'''Late work is penalized 1/3 of a letter grade each day late.''' #Only work turned in on time is guaranteed timely or extensive feedback.
    7 KB (885 words) - 13:41, 29 July 2015
  • 20.109(F12): Mod 1 Day 5 Examine candidate clones & tissue culture
    ...faculty has set up four overnight LB + AMP cultures of bacteria for you to work with. Three of these cultures have colonies from the ligation reactions you ... enzymes you’ll use. The following table may be helpful as you plan your work.
    13 KB (2,328 words) - 13:41, 29 July 2015
  • 20.109(F12): test pages
    ** Late work is penalized 1/3 of a letter grade each day late. ** Only work turned in on time is guaranteed timely or extensive feedback.
    5 KB (689 words) - 13:41, 29 July 2015
  • 20.109(F12): Reflections
    ...9 blog] and you, as students in 20.109, are being asked to reflect on your work in this lab class and then share your impressions. *Future directions you can imagine for the work
    3 KB (470 words) - 13:41, 29 July 2015
  • 20.109(F12):Lab basics
    *Procedures for doing tissue culture work =Communicating your work=
    2 KB (204 words) - 13:41, 29 July 2015
  • 20.109(F12):General lab policy
    ==Late Work== ...ade each day late and will not be accepted after a week.</font color> Only work turned in on time is guaranteed timely or extensive feedback.
    3 KB (478 words) - 13:41, 29 July 2015
  • 20.109(F12):Guidelines for working in the tissue culture facility
    ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.
    10 KB (1,639 words) - 13:41, 29 July 2015
  • 20.109(F12):Guidelines for oral presentations
    ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.
    6 KB (925 words) - 13:41, 29 July 2015
  • 20.109(F12): Oral presentation of research proposal
    * needed resources to complete the work
    2 KB (345 words) - 13:41, 29 July 2015
  • 20.109(F12): Slides for Journal Club Presentation
    ...what the study contributed to the field. Maybe there's been some follow up work worth noting?
    3 KB (512 words) - 13:41, 29 July 2015
  • 20.109(F12):Guidelines for writing up your research
    ...ist the name of your partner on your report since (s)he contributed to the work. Indicate that you are the primary author of the report by underlining your ... trends, raw data (like a picture of a gel), or a model that explains your work.
    19 KB (3,038 words) - 13:41, 29 July 2015
  • 20.109(F12): Mod 1 Day 1 DNA engineering using PCR
    ... establish good habits for documentation of your work. By documenting your work according to the exercises done today, you will ...re there are no spaces between or after the letters or your search won’t work. Change the color of the start codon to blue. Repeat the “Find…” proc
    22 KB (3,714 words) - 13:41, 29 July 2015
  • 20.109(F12):Guidelines for editing OpenWetWare
    ==Sign your work==
    3 KB (461 words) - 13:41, 29 July 2015
  • 20.109(F12): Mod 3 Day 2 Phage nanowires
    ...st with ethanol, then with water. You will have time during these steps to work on the research proposal idea you've got started with your lab partner. Today's lab has some safety hazards and you must work extremely carefully. Lab coats, gloves and goggles are a must when you're a
    10 KB (1,590 words) - 13:41, 29 July 2015
  • 20.109(F12): FACS data analysis and defense
    ...utes and will take place during lab on M2D1, so you will have to plan your work accordingly.
    3 KB (584 words) - 13:41, 29 July 2015
  • 20.109(F12): Lab certifications
    *You will have the print-out of the lab tour to work with but not the computers. *You will have to show your work for any calculations you perform, though you can use a calculator for compu
    2 KB (345 words) - 13:41, 29 July 2015
  • 20.109(F12): Results and Discussion sections
    *conclusions you can draw from your work, including any uncertainties *limitations of your work, e.g. what kinds of experiments/controls/samples would have been great to i
    3 KB (482 words) - 13:41, 29 July 2015
  • 20.109(F12): Online cloning lab and defense
    ... teaching faculty...and so in that sense this is a more "real" view to the work you'll do when you leave our 20.109 class!<br> *Your laboratory work area is the green counter top called the workbench.
    4 KB (767 words) - 13:41, 29 July 2015
  • 20.109(F12): Mod 1 Day 3 Agarose gel electrophoresis
    Ligations generally work best when there is a 1:4 ratio of backbone to insert. You will ligate your
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  • 20.109(F12): Mod 1 Day 4 DNA ligations and bacterial transformations
    ...t.edu/environment/ehs/about_ehs.html (EHS)] come to discuss tissue culture work with you. * work best in the same buffer and at the same temperature.<br>
    12 KB (1,926 words) - 13:41, 29 July 2015
  • 20.109(F12):Module 2
    ...---have lagged behind in the design and construction of novel systems that work in reliable, scalable, intuitive ways. Biology simply does not always perfo
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  • 20.109(F12): Mod 2 Day 2 Measuring system performance
    ... to make accessory pigments needed for the light-sensing fusion protein to work. These phycobilins are shown in the box at the left of the signaling pathwa ... your slides]] that describe your journal club article to the Stellar home-work dropbox that's [http://stellar.mit.edu/S/course/20/fa12/20.109/ here.] Plea
    22 KB (3,562 words) - 13:41, 29 July 2015
  • 20.109(F12): Mod 3 Day 4 Solar cell assembly
    The groups not visiting the Belcher lab should use the extra time to work on their research proposals. These will be presented/turned in just one wee
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  • 20.109(F12): Mod 2 Day 1 Testing an engineered biological system
    ...eral scheme for Two Component Signaling]] Fortunately evolution appears to work with motifs and many of these motifs behave in a reasonably modular fashion ...gal assays you ran (best to include your experimental values and show your work for at least one calculation), then answer the following questions:
    15 KB (2,366 words) - 13:41, 29 July 2015
  • 20.109(F12): Mod 2 Day 3 Tools for system engineering
    ...en defined through traditional scientific studies of EnvZ, for example the work from Tom Silhavy's lab( [http://openwetware.org/wiki/PMID:_9721293 ] and [[ ...ld examine the bacterial photograph you set up last time and document your work and your ideas about the experiment.
    30 KB (4,879 words) - 13:41, 29 July 2015
  • 20.109(F12): Mod 2 Day 5 Assessing re-tuned system
    ... research article| research article]] that you will write to describe your work in this experimental module. Your outline should include references that yo
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  • 20.109(F12): Mod 2 Day 6 Readouts of DNA, Protein
    ...el should take approximately one hour to run. During that hour, you should work on part two of today's protocol.<br>
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  • 20.109(F12): Mod 2 Day 7 Readout of Protein
    ...k on your report, ask questions of each other or the teaching faculty, and work on your oral presentation if that's coming up too. ...ts of your choosing, to talk with other groups about their results, and to work on your research article.
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  • 20.109(S13)
    ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of
    5 KB (865 words) - 13:47, 29 July 2015
  • 20.109(S13): Assignments
    Although your lab work will be done in pairs, <font color = CC0000>'''most assignments will be sub ...</font color> We strongly recommend that you plan ahead and space out your work when possible.
    8 KB (1,174 words) - 13:47, 29 July 2015
  • 20.109(S13):Module 2
    ...iscussions during the development of this module, as well as for her prior work in developing a [http://openwetware.org/wiki/20.109:Module_2 related module
    4 KB (555 words) - 13:47, 29 July 2015
  • 20.109(S13):Transcript-level analysis (Day6)
    ...let’s return to thinking about the structure of cartilage for a bit. Our work in this module so far has focused on on the cells themselves (viability and ... diluted antibody per well. Incubate for 90 min (at room temperature), and work on Parts 2 and 3 of today's protocol.
    17 KB (2,748 words) - 13:47, 29 July 2015
  • 20.109(S13):Context-setting and primer design (Day1)
    ...s transmission) is the flu virus. For your safety, all the samples we will work with have been screened to exclude those carrying humanpathogenic flu st You and your partner may work together on the lab practical. (Note: this will not be the case for future
    21 KB (3,355 words) - 13:47, 29 July 2015
  • 20.109(S13):DNA extraction (Day2)
    Returning to today's specific work, each of you will extract a DNA pool from a single bird stool sample using #*Work quickly and keep the sample on your ice bucket until you have finished addi
    13 KB (2,078 words) - 13:47, 29 July 2015
  • 20.109(S13):PCR and paper discussion (Day3)
    ...ts low viscosity means that high weight percent solutions are tractable to work with, and that the solidified gel remains pliable rather than brittle. HR a ... the work you do in Module 1 in a formal lab report. To help you pace your work, as well as give you feedback early on, you will be required to draft small
    17 KB (2,771 words) - 13:47, 29 July 2015
  • 20.109(S13):DNA cloning (Day4)
    ... leftover ligation mix today. These will be stored frozen in case of later needs.''' ...ke your teaching faculty very happy if you contribute to their preparatory work.
    16 KB (2,679 words) - 13:47, 29 July 2015
  • 20.109(S13):DNA sequencing and primer analysis (Day5)
    ... many colonies, today we will use a commercially available kit so that the work can go more quickly. The principle is the same as that of our "quick and di In another week you will finally get to see the results of all your hard work!
    16 KB (2,629 words) - 13:48, 29 July 2015
  • 20.109(S13):Phylogenetic analysis (Day7)
    #*Why is it more convenient to work with the reverse complement when sequencing from the reverse direction? ...e from each other's text files and Excel write-ups -- but that's twice the work!
    17 KB (2,753 words) - 13:48, 29 July 2015
  • 20.109(S13):General lab policy
    ...mdash; especially your partner &mdash; if you are not up to speed when the work begins. #Do not work with chemicals until you are sure of their safe handling. Be aware of their
    3 KB (445 words) - 13:48, 29 July 2015
  • 20.109(S13):Statement on collaboration and integrity
    ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it.
    2 KB (315 words) - 13:48, 29 July 2015
  • 20.109(S13):Characterize protein expression (Day7)
    ... at a time will work on Part 2. While you wait for your turn, feel free to work on the FNT, which is a high-level outline of your methods section. #Now work your way from reservoirs #2 to #12 (highest calcium concentration), and fro
    11 KB (1,743 words) - 13:48, 29 July 2015
  • 20.109(S13):Initiate cell culture (Day2)
    ...eir knee joints is more similar to that of humans. In this module, we will work with an ''in vitro'' culture model of cartilage-forming cells. Half the class at a time will work in the tissue culture room today. Today will be physically and mentally lab
    9 KB (1,562 words) - 13:48, 29 July 2015
  • 20.109(S14)
    ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of ...rotocol (includes important change to table!). Part 2 of the protocol is a work in progress, as is the FNT and Reagent List [http://openwetware.org/wiki
    8 KB (1,263 words) - 13:58, 29 July 2015
  • 20.109(S14):Microbial DNA extraction (Day1)
    ...s transmission) is the flu virus. For your safety, all the samples we will work with have been screened to exclude those carrying humanpathogenic flu st Returning to today's specific work, each of you will extract a DNA pool from a single bird cloacal sample usin
    16 KB (2,542 words) - 13:58, 29 July 2015
  • 20.109(S14):Diagnostic primer design (Day2)
    #*'''Note that the ''E hellem'' DNA that you will work with is the AF118143.''' #*'''Note that ''V corneae'' DNA that you will work with is indeed from the Broad.'''
    19 KB (3,074 words) - 13:58, 29 July 2015
  • 20.109(S14):16S PCR and paper discussion (Day3)
    ...ts low viscosity means that high weight percent solutions are tractable to work with, and that the solidified gel remains pliable rather than brittle. HR a ...:Microbiome_summary | abstract and summary report]]. To help you pace your work, as well as give you feedback early on, you will be required to draft small
    19 KB (3,108 words) - 13:58, 29 July 2015
  • 20.109(S14):Initiating transcript and protein assays (Day5)
    ... Buffer to the plate. Wait another 60-90 minutes. In the meantime, you can work on cell viability analysis (first listed on Day 4 Part 4). ...of RNA in your cDNA reaction, a 5-10x further dilution of your cDNA should work well.
    17 KB (2,872 words) - 13:58, 29 July 2015
  • 20.109(S14):Phylogenetic and primer analyses (Day7)
    #*''Why is it more convenient to work with the reverse complement when sequencing from the reverse direction?'' ...d sample ID) at this stage. Or, if you are at very different stages of the work, you may sequentially prepare and combine alignment files.</font color>
    17 KB (2,730 words) - 13:58, 29 July 2015
  • 20.109(S14):Reflection assignments summary page
    ... this revision that you think are generalizable and will translate to your work on the second report? Note: If you choose not to revise the module 1 report ... you predicted would be important back then were you able to apply to your work in module 2? As sub-questions, you might consider: What aspects of report-w
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  • 20.109(S14):DNA cloning (Day4)
    ...ke your teaching faculty very happy if you contribute to their preparatory work.
    15 KB (2,521 words) - 13:58, 29 July 2015
  • 20.109(S14):DNA sequencing (Day5)
    ... many colonies, today we will use a commercially available kit so that the work can go more quickly. The principle is the same as that of our "quick and di In another week you will finally get to see the results of all your hard work!
    17 KB (2,778 words) - 13:58, 29 July 2015
  • 20.109(S14): Assignments
    Although your lab work will be done in pairs, <font color = CC0000>'''most assignments will be sub ...</font color> We strongly recommend that you plan ahead and space out your work when possible. For assignments that may be revised, be sure to read the lat
    9 KB (1,307 words) - 13:58, 29 July 2015
  • 20.109(S14):Guidelines for maintaining your lab notebook
    ...onic notebooks, which we believe are easier and more accessible for you to work with. Specifically, we will use Evernote, a free-ware that is installed on ... to the TA for that module only, in order to provide a time-stamp for your work.
    8 KB (1,243 words) - 13:58, 29 July 2015
  • 20.109(S14):Microbiome summary
    ...igures and supporting text &ndash; including context for understanding the work’s broader implications. ...; of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    14 KB (2,322 words) - 13:58, 29 July 2015
  • 20.109(S14):Design experiment (Day1)
    ... of the surrounding environment on cell phenotype. In particular, you will work with primary chondrocytes and/or mesenchymal stem cells in 3D gel culture. ...er time. We will also have some mesenchymal stem cells for a few groups to work with in order to investigate conditions that promote chondrogenesis. '''Ple
    9 KB (1,390 words) - 13:58, 29 July 2015
  • 20.109(S14):Statement on collaboration and integrity
    ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it.
    2 KB (320 words) - 13:58, 29 July 2015
  • 20.109(S14):Protein-level and wrap-up analysis (Day7)
    ...nce they will undergo partly different manipulations. You may also want to work on two separate worksheets, one for each collagen type.
    6 KB (1,026 words) - 13:58, 29 July 2015
  • 20.109(S14):OWW in 20.109
    ===Sign your work===
    9 KB (1,539 words) - 13:58, 29 July 2015
  • 20.109(S14):General lab policy
    ...ndash; especially your partner &ndash; if you are not up to speed when the work begins. #Do not work with chemicals until you are sure of their safe handling. Be aware of their
    3 KB (528 words) - 13:58, 29 July 2015
  • 20.109(S14):Guidelines for oral presentations
    ...ts &ndash; including organization, clarity, and proper attribution for the work. *Generally speaking, you should figure out how to work the lights, slide projector, curtains, etc. before you begin. In this class
    6 KB (917 words) - 13:58, 29 July 2015
  • 20.109(S14):Initiate cell culture (Day2)
    ...eir knee joints is more similar to that of humans. In this module, we will work with an ''in vitro'' culture model of cartilage-forming cells. Half the class at a time will work in the tissue culture room today. Today will be physically and mentally lab
    9 KB (1,550 words) - 13:59, 29 July 2015
  • 20.109(S14):Introduction to cell strains and plating (Day1)
    ...ross which you will compare repair: cell type and damage topology. We will work exclusively with Chinese hamster ovary (CHO) cells; wild-type cells are cal ...eur pipets and pipet tips can go directly into the sharps mayo jars as you work. Please set aside serological pipets, conical tubes, tissue culture dishes,
    17 KB (2,764 words) - 13:59, 29 July 2015
  • 20.109(S14):Complete Western and prepare damaged DNA (Day4)
    ...don't need to measure out this volume. Keep in mind that the washing steps work by dilution, so it is a balance between adding enough to create a sink for
    17 KB (2,797 words) - 13:59, 29 July 2015
  • 20.109(S14):Cell preparation for DNA repair assays (Day5)
    While your compatriots work in TC, you can: take a break, work on your Mod 1 revision, re-check your lipofection calculations, grab a snac
    9 KB (1,535 words) - 13:59, 29 July 2015
  • 20.109(S14):DNA repair assays(Day6)
    #*As you work, tip the plate down a little to pool the media at the bottom of each well. ... We will have one aliquot per team on T/R, and a different distribution of work or duplicate counting on W/F. Some reminders:
    17 KB (2,912 words) - 13:59, 29 July 2015
  • 20.109(S14):Data analysis (Day7)
    ...your perspective &ndash; it turns out in scientific research that the hard work is just beginning once the data is quantified! Interpreting the data and dr The one piece of wet lab work that you will do today is completing the C401 validation assay. Clonogenic
    16 KB (2,686 words) - 13:59, 29 July 2015
  • 20.109(S14):Testing cell viability (Day3)
    ...of your labmates time.''' Reading the protocol in advance will help you work more quickly, and is strongly recommended. When observing your cells under fluorescence excitation, you should work with the room lights off for best results. A member of the teaching faculty
    15 KB (2,507 words) - 13:59, 29 July 2015
  • 20.109 Main Page
    ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of
    2 KB (292 words) - 20:05, 13 January 2023
  • Review of probability concepts
    This quick review of probability concepts is a work in progress. ...hink about and certainly annoying to work with. It's frequently cleaner to work with intervals. The chance that a random variable will fall ''between'' two
    7 KB (1,214 words) - 21:12, 31 January 2019
  • Compound Microscope Example
    C) Will your microscope still work if we change the distance between the lenses? C) Will your microscope still work if we change the distance between the lenses?
    2 KB (359 words) - 17:09, 12 August 2015
  • Relay Lens Example
    A) Trace the rays to check her work and see where the image is formed.
    1 KB (206 words) - 17:09, 12 August 2015
  • Beam Expander Example
    We will only set up the diagram here. The rest will be left to you to work out. Remember that a good practice is to put the curved side of the lens to
    3 KB (397 words) - 17:10, 12 August 2015
  • 20.109(F15)
    ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of
    3 KB (448 words) - 14:08, 4 November 2015
  • 20.109(F15):Lab basics
    *Procedures for doing tissue culture work =Communicating your work=
    1 KB (185 words) - 11:40, 4 September 2015
  • 20.109(F15):Module 2
    ...cussions during the development of this module, as well as for their prior work in developing a [http://openwetware.org/wiki/20.109:Module_2 related module
    4 KB (566 words) - 11:40, 4 September 2015
  • 20.109(F15):Module 3
    ...863670 CNN videotape of Angie Belcher teaching President Obama] about this work!
    3 KB (392 words) - 15:14, 11 December 2015
  • 20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)
    ...s wasting a month of your time repeating experiments already proven not to work or reinventing the wheel. ...As you work, you can ask yourself why these stretches of the protein might work the way that they do, and how they might be changed.
    28 KB (4,525 words) - 14:45, 12 November 2015
  • 20.109(F15):Agarose gel electrophoresis (Day3)
    Ligations generally work best when there is a 1:4 '''molar''' ratio of backbone to insert. You will
    11 KB (1,795 words) - 19:08, 23 September 2015
  • 20.109(F15):Site-directed mutagenesis (Day2)
    ...al of information in order to design mutagenized inverse pericams nice work! Today you will put your designs into practice. ...reaction, to ensure that all the reagents are working properly. You should work quickly but carefully, and keep your tube in a chilled container at all tim
    14 KB (2,182 words) - 11:40, 4 September 2015
  • 20.109(F15):Bacterial amplification of DNA (Day3)
    ...work on Parts 3 and (optional) 5, label the tubes you will need in Part 4, work on your notebooks, start the FNT assignment (it's a little long for a weekd '''Please clearly show all your work and reasoning throughout this assignment. Parts A through C should be compl
    13 KB (2,207 words) - 11:40, 4 September 2015
  • 20.109(F15):Prepare expression system (Day2)
    #Incubate on ice for 1 hour. You can work on parts 2 and 4 of today's protocols now, as well as assemble the material #*How does the chimeric protein pericam work as a sensor? (No need for details about different types of pericams yet.)
    19 KB (3,167 words) - 20:42, 14 October 2015
  • 20.109(F15):Induce protein and evaluate DNA (Day3)
    As evidenced by Nagai’s work, wild-type inverse pericam is not toxic to BL21(DE3)pLysS cells. Although i ...u induce your cells with IPTG, you will let the protein factories do their work for 2-3 hours. During this time, you will evaluate the DNA from your two X#
    16 KB (2,649 words) - 20:22, 21 October 2015
  • 20.109(F15):General lab policy
    ==Late work== ...ade each day late and will not be accepted after a week.</font color> Only work turned in on time is guaranteed timely or extensive feedback.
    3 KB (479 words) - 12:13, 8 September 2015
  • 20.109(F15): DNA engineering methods
    ... for this assignment, but you will receive substantive feedback about your work from a laboratory instructor. ...e, not the DNA repair assay phase. In other words, you should describe the work from M1D1-D5, NOT including tissue culture.
    3 KB (408 words) - 11:40, 4 September 2015
  • 20.109(F15):Purify protein (Day4)
    ... has 15 &mu;L of liquid in it. You might use the table below to guide your work. #*The timing function on this centrifuge does not work! Bring your timer and manually turn the centrifuge off after 2 minutes. Sta
    13 KB (2,001 words) - 18:01, 11 November 2015
  • 20.109(F15):Characterize protein expression (Day6)
    ... its lengthy incubation steps. After that, about two groups at a time will work on Part 2. #Now work your way from reservoirs #2 to #12 (highest calcium concentration), and fro
    10 KB (1,581 words) - 21:42, 10 November 2015
  • 20.109(F15):Assess protein function (Day7)
    ...got last time. Begin by analyzing the wild-type protein as a check on your work (your curve should resemble Nagai's Figure 3L), and then move on to your mu ...t be discouraged if your wild-type values do not exactly match Nagai’s work, or if there is variation between Parts 1, 2, and 3.
    15 KB (2,549 words) - 00:24, 3 November 2015
  • 20.109(F15): TA notes for module 1
    * Make a plan for preparing the high volume TC work we do. Lab also needs you to:
    16 KB (2,554 words) - 17:19, 12 January 2016
  • 20.109(F15):Growth of phage materials (Day1)
    ...ientists who can tolerate a mix of p8 proteins on the phage coat for their work, there are phage-display variations that mix and match fusion and wild-type ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (''e.g'' see reference [http://www.sciencemag.org/cgi/content/abstract/312/
    15 KB (2,551 words) - 14:26, 16 November 2015
  • 20.109(F15):TEM (Day4)
    ===How does a TEM work?=== #Use the downtime to work on your [[20.109(F15): Biomaterials engineering report| Biomaterials engine
    5 KB (762 words) - 17:07, 28 November 2015
  • 20.109(F15):DNA engineering using PCR (Day1)
    ...e is for you to establish good habits concerning the documentation of your work. These good habits will allow you to: ...re there are no spaces between or after the letters or your search won’t work. Change the color of the start codon to blue. Repeat the “Find…” proc
    21 KB (3,638 words) - 13:47, 2 October 2015
  • 20.109(F15):DNA ligation & transformation (Day4)
    ... will confirm the presence of the PCR insert. Please clearly show all your work and reasoning in your lab notebook, and be sure that your plan explicitly i * work best in the same buffer and at the same temperature
    11 KB (1,745 words) - 20:32, 24 September 2015
  • 20.109(F15):Guidelines for writing up your research
    ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed. ...motivate the audience to keep reading! How? Reveal the significance of the work through connections to both prior scientific accomplishments and interestin
    21 KB (3,266 words) - 11:40, 4 September 2015
  • 20.109(F15):Guidelines for working in the tissue culture facility
    ...ut most are common practice and will be good habits for any tissue culture work you do. ...best with the blowers off when not in use. If you are the first person to work in the hood, turn the blower on, open the sash, and wait 10 minutes before
    7 KB (1,217 words) - 11:40, 4 September 2015
  • 20.109(F15):Examine candidate clones & tissue culture (Day5)
    ...g faculty has set up four overnight LB+AMP cultures of bacteria for you to work with. Three of these cultures have colonies from the ligation reactions you ... enzymes you’ll use. The following table may be helpful as you plan your work. Each enzyme should be present in 2.5 U quantity. (As an example, the ''X
    21 KB (3,522 words) - 18:18, 30 September 2015
  • 20.109(F15):Guidelines for oral presentations
    ... elements, including organization, clarity, and proper attribution for the work. *Generally speaking, you should figure out how to work the lights, slide projector, curtains, etc. before you begin. In this class
    5 KB (896 words) - 11:40, 4 September 2015
  • 20.109(F15):Phage nanowires (Day2)
    ... gold and silver. You will have time while these reactions are going on to work on the research proposal idea you've got started with your lab partner.
    5 KB (893 words) - 18:32, 3 November 2015
  • 20.109(F15):Statement on collaboration and integrity
    ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it.
    2 KB (320 words) - 11:40, 4 September 2015
  • 20.109(F15): Assignments
    Although your lab work will be done in pairs, <font color = CC0000>'''most assignments will be sub ...</font color> We strongly recommend that you plan ahead and space out your work when possible. For assignments that may be revised, be sure to read the lat
    9 KB (1,370 words) - 22:06, 25 November 2015
  • 20.109(F15):20.109 Class Blog summary page
    ...first, as it refreshes my memory and reminds me how exciting it was do the work. Next, I take this ‘writers high’ to the data section, which I find the
    6 KB (1,007 words) - 13:06, 15 October 2015
  • 20.109(F15): Protein engineering report
    ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion. ...y appropriate citations. '''Any details you found in another researcher's work should be cited.'''
    8 KB (1,190 words) - 18:12, 9 November 2015
  • 20.109(F15): Biomaterials engineering report
    ...summarize and interpret the class-wide findings and briefly suggest future work. What we usually call Results and Discussion should be combined. The Method |Contextualizing Results and Suggestions for Future Work
    4 KB (593 words) - 17:06, 30 November 2015
  • 20.109(F15):Journal Club II (Day8)
    ...s focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. R ...ease email it (as .pdf) to your instructor with a brief description of the work.
    12 KB (1,779 words) - 04:42, 3 November 2015
  • 20.109(F15): DNA engineering summary
    ...in figures and supporting text – including context for understanding the work’s broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    10 KB (1,486 words) - 16:41, 7 October 2015
  • 20.109(F15):Wiki basics
    ===Sign your work===
    9 KB (1,474 words) - 12:35, 10 September 2015
  • 20.109(F15):Homework
    ...ur computer versus operating from the web based application (although both work). You can also download Evernote to your smart phone for ease of uploading #*You may work on paper or electronically. Either way, prepare a schematic rather than det
    28 KB (4,514 words) - 20:04, 2 December 2015
  • 20.109(F15):Research proposal presentation
    * needed resources to complete the work ...n this proposal. For example, if you propose molecular and/or ''in vitro'' work, you would want to be able to say a few words about ''in vivo'' experiments
    14 KB (2,363 words) - 18:50, 30 November 2015
  • 20.109(F15):Battery assembly (Day3)
    Today's lab protocol will require that we work in small groups in the Belcher lab. You will use their balance to measure v ...neral approach to the problem. Appropriate justification for the proposed work can be provided through literature citations, data from other sources, or f
    7 KB (1,140 words) - 11:40, 4 September 2015
  • 20.109(F15): DNA engineering presentation
    ...erm "elevator pitch" is used to describe a brief oral presentation of your work; it suggests that you have the time of an elevator ride to explain the impo ...uld both introduce your research project and convey the importance of your work in the context of the field. You want the listener to understand why your
    5 KB (708 words) - 13:23, 17 October 2015
  • 20.109(F15):TEM (Day5)
    #Use the downtime to work on your Biomaterials engineering mini-report or your Research proposal pres
    3 KB (438 words) - 11:40, 4 September 2015
  • 20.109(F15):Purify active materials (Day2)
    ...isms generate minerals that are often used to harden soft tissues. In our work for module 3 we are using M13 as a template for the biomineralization of ph
    6 KB (932 words) - 18:56, 17 November 2015
  • 20.109(F15):Cathode construction (Day3)
    ...ate, which may impede the current flow required for the battery to perform work. ...heir time, equipment, and space. Because you will be completing laboratory work in the Belcher Lab, it is important that you take goggles and a lab coat wi
    10 KB (1,568 words) - 21:55, 18 November 2015
  • 20.109(S16):Module 3
    ...863670 CNN videotape of Angie Belcher teaching President Obama] about this work!
    3 KB (391 words) - 19:48, 9 April 2016
  • 20.109(S16)
    ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of
    2 KB (403 words) - 17:54, 5 May 2016
  • 20.109(S16):Statement on collaboration and integrity
    ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it.
    2 KB (320 words) - 14:49, 9 December 2015
  • 20.109(S16): Assignments
    Although your lab work will be done in pairs, <font color = CC0000>'''most assignments will be sub ...</font color> We strongly recommend that you plan ahead and space out your work when possible. For assignments that may be revised, be sure to read the lat
    9 KB (1,346 words) - 18:05, 6 April 2016
  • 20.109(S16):20.109 Class Blog summary page
    ...first, as it refreshes my memory and reminds me how exciting it was do the work. Next, I take this ‘writers high’ to the data section, which I find the
    6 KB (1,008 words) - 19:24, 28 March 2016
  • 20.109(S16):Guidelines for oral presentations
    ... elements, including organization, clarity, and proper attribution for the work. *Generally speaking, you should figure out how to work the lights, slide projector, curtains, etc. before you begin. In this class
    5 KB (896 words) - 16:07, 9 December 2015
  • 20.109(S16): Protein engineering summary
    ...in figures and supporting text – including context for understanding the work’s broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    9 KB (1,392 words) - 20:18, 29 February 2016
  • 20.109(S16):Guidelines for writing up your research
    ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed. ...motivate the audience to keep reading! How? Reveal the significance of the work through connections to both prior scientific accomplishments and interestin
    21 KB (3,265 words) - 15:35, 7 April 2016
  • 20.109(S16): Protein engineering presentation
    ...erm "elevator pitch" is used to describe a brief oral presentation of your work; it suggests that you have the time of an elevator ride to explain the impo ...uld both introduce your research project and convey the importance of your work in the context of the field. You want the listener to understand why your
    4 KB (661 words) - 19:42, 15 February 2016
  • 20.109(S16):Journal Club II (Day8)
    ...s focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. R ...ease email it (as .pdf) to your instructor with a brief description of the work.
    10 KB (1,596 words) - 18:50, 2 April 2016
  • 20.109(S16): System engineering report
    ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion. ...e citations. '''Any and all information you found in another researcher's work should be cited.''' A failure to cite information you did not innately kno
    11 KB (1,619 words) - 16:17, 14 April 2016
  • 20.109(S16):Research proposal presentation
    * needed resources to complete the work ...n this proposal. For example, if you propose molecular and/or ''in vitro'' work, you would want to be able to say a few words about ''in vivo'' experiments
    12 KB (1,985 words) - 17:47, 25 April 2016
  • 20.109(S16): Biomaterials engineering report
    ...summarize and interpret the class-wide findings and briefly suggest future work. What we usually call Results and Discussion should be combined. The Method |Contextualizing Results and Suggestions for Future Work
    4 KB (558 words) - 16:55, 28 April 2016
  • 20.109(S16):Lab basics
    *Procedures for doing tissue culture work =Communicating your work=
    1 KB (185 words) - 16:08, 9 December 2015
  • 20.109(S16):General lab policy
    ==Late work== ...ade each day late and will not be accepted after a week.</font color> Only work turned in on time is guaranteed timely or extensive feedback.
    3 KB (479 words) - 16:09, 9 December 2015
  • 20.109(S16):Guidelines for working in the tissue culture facility
    ...ut most are common practice and will be good habits for any tissue culture work you do. ...best with the blowers off when not in use. If you are the first person to work in the hood, turn the blower on, open the sash, and wait 10 minutes before
    7 KB (1,217 words) - 16:21, 9 December 2015
  • 20.109(S16):OWW basics
    ===Sign your work===
    9 KB (1,478 words) - 16:25, 9 December 2015
  • 20.109(S16):Growth of phage materials (Day1)
    ...ientists who can tolerate a mix of p8 proteins on the phage coat for their work, there are phage-display variations that mix and match fusion and wild-type ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (''e.g'' see reference [http://www.sciencemag.org/cgi/content/abstract/312/
    16 KB (2,684 words) - 18:48, 15 April 2016
  • 20.109(S16):Purify active materials (Day2)
    ...isms generate minerals that are often used to harden soft tissues. In our work for module 3 we are using M13 as a template for the biomineralization of ph
    6 KB (1,017 words) - 21:58, 21 April 2016
  • 20.109(S16):Cathode construction (Day3)
    ...ate, which may impede the current flow required for the battery to perform work. ...heir time, equipment, and space. Because you will be completing laboratory work in the Belcher Lab, it is important that you take goggles and a lab coat wi
    9 KB (1,567 words) - 22:07, 10 December 2015
  • 20.109(S16):TEM (Day4)
    ===How does a TEM work?=== #Use the downtime to work on your [[20.109(S16): Biomaterials engineering report| Biomaterials engine
    5 KB (762 words) - 15:35, 19 April 2016
  • 20.109(S16):Introduction to cell strains and plating (Day1)
    ...cells for Western blot analysis. To ensure you are well-prepared for your work in the tissue culture room, your instructor will deliver the prelab informa
    16 KB (2,780 words) - 17:34, 19 September 2016
  • Measuring biological forces mini-lab
    [[Category:Needs Work]]
    4 KB (594 words) - 18:00, 29 August 2016
  • 20.109(S16):Begin Western blot protein analysis and choose system conditions (Day2)
    ...te!''' We will store these samples at -80 &deg;C in case your Western blot needs to be repeated. #During electrophoresis, work through Part 5 with your laboratory partner.
    21 KB (3,393 words) - 15:00, 11 March 2016
  • 20.109(S16):Complete Western and prepare damaged DNA (Day3)
    #*Keep in mind that the washing steps work by dilution, so it is a balance between adding enough to create a sink for
    16 KB (2,558 words) - 15:49, 1 April 2016
  • 20.109(S16):Cell preparation for DNA repair assays (Day5)
    ...sue culture room. Please note the following procedural details before you work through the math: ...alert the teaching faculty that you are ready to begin your tissue culture work.
    16 KB (2,542 words) - 18:34, 28 March 2016
  • 20.109(S16):DNA repair assays (Day6)
    ...re you are familiar with how the data are collected. The samples you will work with today are the controls used to designate the gates that enable the flo
    14 KB (2,303 words) - 14:32, 14 April 2016
  • 20.109(S16):Flow cytometry and paper discussion (Day7)
    ...your perspective &ndash; it turns out in scientific research that the hard work is just beginning once the data is quantified! Interpreting the data and dr
    11 KB (1,862 words) - 13:19, 4 April 2016
  • 20.109(S16):Data analysis (Day9)
    The final experimental work that you will do for this module is to complete your NHEJ inhibitor kill cu
    7 KB (1,170 words) - 16:31, 11 April 2016
  • 20.109(S16):In situ cloning (Day1)
    '''Be sure to document your work and answer all questions in your lab notebook as you progress through the e ...nce information to your notebook (it may be easiest to screen capture your work station in APE and embed the image in your notebook).
    22 KB (3,676 words) - 21:29, 4 February 2016
  • 20.109(S16):Design mutation primers (Day2)
    ... a month of your time repeating experiments previously demonstrated not to work or reinventing the wheel. ...As you work, you can ask yourself why these stretches of the protein might work the way that they do, and how they might be changed.
    23 KB (3,783 words) - 20:41, 9 February 2016
  • 20.109(S16):Site-directed mutagenesis (Day3)
    ...reaction, to ensure that all the reagents are working properly. You should work quickly but carefully, and keep your tube in a chilled container at all tim #*How does the chimeric protein pericam work as a sensor? (No need for details about different types of pericams yet.)
    13 KB (2,066 words) - 14:08, 17 February 2016
  • 20.109(S16):Prepare expression system (Day4)
    #Incubate on ice for 1 h. You can work on Parts 2, 3 and 5 of today's protocols now, as well as assemble the mater ...ant to remove all traces of ethanol, as they may interfere with subsequent work with the DNA.
    17 KB (2,817 words) - 22:14, 21 February 2016
  • 20.109(S16):Homework
    ...ur computer versus operating from the web based application (although both work). You can also download Evernote to your smart phone for ease of uploading ...e fragment sizes expected following the double-digest reaction. Show your work here!
    25 KB (3,974 words) - 17:41, 5 May 2016
  • 20.109(S16):Purify protein (Day6)
    ... has 15 &mu;L of liquid in it. You might use the table below to guide your work. #*The timing function on this centrifuge does not work! Bring your timer and manually turn the centrifuge off after 2 minutes. Sta
    14 KB (2,249 words) - 20:15, 26 February 2016
  • 20.109(S16):Characterize protein expression (Day7)
    ... its lengthy incubation steps. After that, about two groups at a time will work on Part 2. #Now work your way from reservoirs #2 to #12 (highest calcium concentration), and fro
    10 KB (1,586 words) - 14:39, 26 February 2016
  • 20.109(S16):Assess protein function (Day8)
    ...got last time. Begin by analyzing the wild-type protein as a check on your work (your curve should resemble Nagai's Figure 3L), and then move on to your mu ...t be discouraged if your wild-type values do not exactly match Nagai’s work, or if there is variation between Parts 1, 2, and 3.
    15 KB (2,565 words) - 20:14, 2 March 2016
  • 20.109(S16):Module 1
    ...cussions during the development of this module, as well as for their prior work in developing a [http://openwetware.org/wiki/20.109:Module_2 related module
    4 KB (614 words) - 15:19, 6 March 2016
  • 20.109(S16):Module 2
    ...l line lacking a subunit of DNA-PK, M059K and M059J, respectively. As you work through this module you will gain additional skills in analyzing and commun
    4 KB (656 words) - 16:45, 5 February 2016
  • 20.109(F16): Fall 2016 schedule
    ... at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of
    10 KB (1,559 words) - 18:39, 19 December 2016
  • 20.109(F16):Prepare comet chips (Day1)
    #*Be careful not to introduce bubbles into the agarose and work quickly as the agarose will solidify as it cools.
    12 KB (1,972 words) - 17:32, 19 September 2016
  • 20.109(F16):DNA engineering using PCR (Day1)
    ...e is for you to establish good habits concerning the documentation of your work. These good habits will allow you to: ...re there are no spaces between or after the letters or your search won’t work. Change the color of the start codon to blue. Repeat the “Find…” proc
    21 KB (3,638 words) - 14:58, 18 May 2016
  • 20.109(F16):Lab tour
    ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it. During your work in tissue culture, you will use a hemocytometer to count mammalian cells.
    27 KB (4,439 words) - 21:13, 9 September 2016
  • 20.109(F16):Agarose gel electrophoresis (Day3)
    Ligations generally work best when there is a 1:4 '''molar''' ratio of backbone to insert. You will
    11 KB (1,791 words) - 15:16, 18 May 2016
  • 20.109(F16):DNA ligation & transformation (Day4)
    ... will confirm the presence of the PCR insert. Please clearly show all your work and reasoning in your lab notebook, and be sure that your plan explicitly i * work best in the same buffer and at the same temperature
    11 KB (1,745 words) - 15:25, 18 May 2016
  • 20.109(F16):Examine candidate clones & tissue culture (Day5)
    ...g faculty has set up four overnight LB+AMP cultures of bacteria for you to work with. Three of these cultures have colonies from the ligation reactions you ... enzymes you’ll use. The following table may be helpful as you plan your work. Each enzyme should be present in 2.5 U quantity. (As an example, the ''X
    21 KB (3,522 words) - 15:28, 18 May 2016
  • 20.109(F16):Growth of phage materials (Day1)
    ...ientists who can tolerate a mix of p8 proteins on the phage coat for their work, there are phage-display variations that mix and match fusion and wild-type ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (''e.g'' see reference [http://www.sciencemag.org/cgi/content/abstract/312/
    16 KB (2,648 words) - 21:20, 16 November 2016
  • 20.109(F16):Purify active materials (Day2)
    ...isms generate minerals that are often used to harden soft tissues. In our work for module 3 we are using M13 as a template for the biomineralization of ph
    6 KB (1,002 words) - 18:40, 15 November 2016
  • 20.109(F16):Cathode construction (Day3)
    ...ate, which may impede the current flow required for the battery to perform work. ...heir time, equipment, and space. Because you will be completing laboratory work in the Belcher Lab, it is important that you take goggles and a lab coat wi
    9 KB (1,567 words) - 15:50, 18 May 2016
  • 20.109(F16):TEM (Day4)
    ===How does a TEM work?=== #Use the downtime to work on your [[20.109(F16): Biomaterials engineering mini-report| Biomaterials e
    5 KB (762 words) - 18:35, 8 November 2016
  • 20.109(F16):Statement on collaboration and integrity
    ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it.
    2 KB (317 words) - 19:37, 18 May 2016
  • 20.109(F16): Assignments
    ...nt and in the ways that your learning will be assessed. Although your lab work will be done in pairs, <font color = red>'''most assignments will be submit ...ongly recommend that you plan ahead and and schedule time to complete your work when possible.
    8 KB (1,143 words) - 15:04, 1 November 2016
  • 20.109(F16): DNA engineering summary
    ...in figures and supporting text including context for understanding the works broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    10 KB (1,487 words) - 14:19, 9 June 2016
  • 20.109(F16): DNA engineering presentation
    ...erm "elevator pitch" is used to describe a brief oral presentation of your work; it suggests that you have the time of an elevator ride to explain the impo ...uld both introduce your research project and convey the importance of your work in the context of the field. You want the listener to understand why your
    4 KB (586 words) - 14:21, 9 June 2016
  • 20.109(F16):Research proposal presentation
    * needed resources to complete the work (not a separate slide, integrated when relevant) *describes/credits relevant prior work
    15 KB (2,372 words) - 15:22, 17 November 2016
  • 20.109(F16): Biomaterials engineering mini-report
    ...summarize and interpret the class-wide findings and briefly suggest future work. What we usually call Results and Discussion should be combined. The Method |Contextualizing Results and Suggestions for Future Work
    4 KB (530 words) - 16:23, 6 December 2016
  • 20.109(F16):Homework
    ... the four potential topic bullets listed under the Implications and Future Work content guidelines. ...ary#Implications_and_Future_Work:_potential_topics Implications and Future Work] suggestions provided for the M1 Data Summary.
    21 KB (3,289 words) - 21:26, 30 November 2016
  • 20.109(F16):Communication
    You will use an online laboratory notebook to document your work in 20.109. [http://benchling.com/ Benchling] is a free program that allows ... elements, including organization, clarity, and proper attribution for the work.
    30 KB (4,506 words) - 22:00, 30 November 2016
  • Optical Microscopy Part 3: Resolution, Stability, and Particle Tracking
    ...iments of J. Perrin that lead to his Nobel Prize. A translation of the key work is included in the reprints folder. Have a look – he used latex spheres, ...ycerine%20Solutions.pdf chart] is a useful reference. If that link doesn't work try [http://profs.engineering.uottawa.ca/biofluid/files/2011/08/viscosity-o
    16 KB (2,559 words) - 22:47, 15 March 2017
  • 20.109(F16):Test comet chip loading variables (Day2)
    ...d by Lizzie Ngo from the Engelward Laboratory. Please note that your hard work from our last laboratory section was not in vain! The CometChip you prepar #*You will need to work quickly from this point as the LMP agarose will solidify as it cools.
    15 KB (2,594 words) - 17:33, 22 September 2016
  • 20.109(F16):Seed cells for immuno-flourescence assay (Day5)
    ... excisions, abasic sites, strand breaks, and crosslinks. The experimental work you complete in the next three laboratory classes will focus on a technique ...below will be completed in the tissue culture room. Before you begin your work at the biosafety cabinet, the teaching faculty will lead you through a demo
    12 KB (2,013 words) - 20:08, 30 September 2016
  • 20.109(F16):Design gRNA for CRISPRi (Day2)
    ...mine the results of your digests and perform the next stage of preparatory work by selecting a target for your metabolism engineering project. Upon determ
    13 KB (2,092 words) - 15:25, 6 October 2016
  • 20.109(F16):Complete in silico cloning (Day1)
    '''Be sure to document your work and answer all questions in your lab notebook as you progress through the e #Be sure to record all of your work for the ligation calculations in your notebook.
    23 KB (3,824 words) - 17:24, 13 October 2016
  • 20.109(F16):Generate gRNA plasmid (Day3)
    ...reaction, to ensure that all the reagents are working properly. You should work quickly but carefully, and keep your tube in a chilled container at all tim
    11 KB (1,747 words) - 20:42, 14 November 2016
  • 20.109(F16):Journal club II (Day 6)
    ...s focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. R ...ease email it (as .pdf) to your instructor with a brief description of the work.
    10 KB (1,515 words) - 16:52, 2 November 2016
  • 20.109(F16):Confirm gRNA sequence (Day5)
    ...ant to remove all traces of ethanol, as they may interfere with subsequent work with the DNA.
    14 KB (2,265 words) - 21:01, 10 November 2016
  • 20.109(F16):Asses DNA repair variability with comet chip (Day4)
    #**You will need to work quickly from this point as the 1% LMP agarose will solidify as it cools.
    8 KB (1,390 words) - 17:23, 27 September 2016
  • 20.109(F16):Measure DNA damage with comet chip (Day3)
    #*You will need to work quickly from this point as the 1% LMP agarose will solidify as it cools. #*Work on a piece of diaper paper at your bench when preparing MMS solutions!
    11 KB (1,892 words) - 19:35, 26 September 2016
  • 20.109(F16): M1 Mini-presentation
    ...erm "elevator pitch" is used to describe a brief oral presentation of your work; it suggests that you have the time of an elevator ride to explain the impo ...uld both introduce your research project and convey the importance of your work in the context of the field. You want the listener to understand why your
    4 KB (647 words) - 18:57, 7 September 2016
  • 20.109(F16): M1 Data Summary
    ...in figures and supporting text – including context for understanding the work’s broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    10 KB (1,449 words) - 12:08, 29 September 2016
  • 20.109(F16): Research article
    ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion. ...y appropriate citations. '''Any details you found in another researcher's work should be cited.'''
    8 KB (1,243 words) - 16:42, 4 November 2016
  • 20.109(S17): Assignments
    ...nt and in the ways that your learning will be assessed. Although your lab work will be done in pairs, <font color = red>'''most assignments will be submit ...ongly recommend that you plan ahead and and schedule time to complete your work when possible.
    8 KB (1,139 words) - 19:22, 10 February 2017
  • 20.109(S17):Lab tour
    ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it.
    24 KB (3,951 words) - 21:35, 31 January 2017
  • 20.109(S17):Journal Club II (Day8)
    ...s focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. R ...ching faculty (Noreen, Leslie, and Maxine) with a brief description of the work.
    12 KB (1,818 words) - 20:50, 6 April 2017
  • 20.109(S17):Guidelines for working in the tissue culture facility
    ...ut most are common practice and will be good habits for any tissue culture work you do. ...best with the blowers off when not in use. If you are the first person to work in the hood, turn the blower on, open the sash, and wait 10 minutes before
    7 KB (1,195 words) - 15:51, 6 September 2017
  • 20.109(S17):Homework
    ...ary#Implications_and_Future_Work:_potential_topics Implications and Future Work] suggestions provided for the Data Summary. ...sketched out; you simply have to describe five recent examples of existing work that you find interesting. However, you can start to brainstorm how to expa
    22 KB (3,470 words) - 18:41, 3 May 2018
  • 20.109(S17):Communication
    You will use an online laboratory notebook to document your work in 20.109. [http://benchling.com/ Benchling] is a free program that allows ... elements, including organization, clarity, and proper attribution for the work.
    30 KB (4,508 words) - 17:29, 28 April 2017
  • 20.109(S17):Growth of phage materials (Day1)
    ...ientists who can tolerate a mix of p8 proteins on the phage coat for their work, there are phage-display variations that mix and match fusion and wild-type ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (''e.g'' see reference [http://www.sciencemag.org/cgi/content/abstract/312/
    16 KB (2,628 words) - 14:28, 24 April 2017
  • 20.109(S17):Purify active materials (Day2)
    ...isms generate minerals that are often used to harden soft tissues. In our work for module 3 we are using M13 as a template for the biomineralization of ph
    6 KB (1,004 words) - 14:34, 24 April 2017
  • 20.109(S17):Cathode construction (Day3)
    ...ate, which may impede the current flow required for the battery to perform work. ...heir time, equipment, and space. Because you will be completing laboratory work in the Belcher Lab, it is important that you take goggles and a lab coat wi
    10 KB (1,573 words) - 22:17, 31 January 2017
  • 20.109(S17):TEM (Day4)
    ===How does a TEM work?=== #Use the downtime to work on your [[20.109(S17): Biomaterials engineering mini-report| Biomaterials e
    5 KB (766 words) - 22:18, 31 January 2017
  • 20.109(S17):Research proposal presentation
    * needed resources to complete the work (not a separate slide, integrated when relevant) *describes/credits relevant prior work
    15 KB (2,372 words) - 19:40, 28 December 2016
  • 20.109(S17): Biomaterials engineering mini-report
    ...summarize and interpret the class-wide findings and briefly suggest future work. What we usually call Results and Discussion should be combined. The Method |Contextualizing Results and Suggestions for Future Work
    4 KB (532 words) - 16:51, 27 April 2017
  • 20.109(S17): M1 Data Summary
    ...in figures and supporting text – including context for understanding the work and insight into the broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    8 KB (1,202 words) - 15:32, 7 February 2017
  • 20.109(S17): M1 Mini-presentation
    ...erm "elevator pitch" is used to describe a brief oral presentation of your work; it suggests that you have the time of an elevator ride to explain the impo ...uld both introduce your research project and convey the importance of your work in the context of the field. You want the listener to understand why your
    4 KB (628 words) - 01:27, 31 January 2017
  • 20.109(S17): Research article
    ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion. ...y appropriate citations. '''Any details you found in another researcher's work should be cited.'''
    8 KB (1,242 words) - 00:09, 4 April 2017
  • 20.109(S17):In silico cloning and induction of protein expression (Day1)
    ...t was cloned by the teaching faculty. In this exercise you will virtually work through the cloning steps that were used to ligate the FKBP12 gene into the '''Be sure to document your work and answer all questions in your lab notebook as you progress through the e
    24 KB (3,850 words) - 19:53, 2 March 2017
  • Assignment 3, Part 1: visualizing actin with fluorescence contrast
    ... to connect phalloidin molecule to a fluorescent dye molecule. But will it work? I won't keep you in suspense any longer. Phalloidin retains its high affin
    15 KB (2,365 words) - 20:25, 25 February 2020
  • 20.109(S17):Scan slides to identify FKBP12 binders (Day5)
    ...e paper. Rather the questions that you address in your experiments should work toward a complete and coherent story.
    5 KB (810 words) - 19:49, 27 February 2017
  • 20.109(S17):Complete data analysis (Day6)
    #*Be sure that a cell within your spreadsheet is highlighted (any cell will work).
    10 KB (1,717 words) - 16:05, 3 March 2017
  • 20.109(S17):Identify chemical structures common among FKBP12 binders
    ...re, mechanics, metabolism, and signaling. In these roles, proteins do not work in a vacuum; rather the biological roles of proteins are dependent on direc ...easures the capacity of a thermodynamic system to do maximum or reversible work at constant temperature and pressure. When at equilibrium with constant te
    5 KB (791 words) - 15:52, 6 March 2017
  • 20.109(S17):Confirm cell lines and practice tissue culture techniques (Day1)
    You will work with these cells, mostly, in our tissue culture room. Tissue culture was d ... treatment following DNA damage. To ensure you are well-prepared for your work in the tissue culture room, your instructor will provide a demonstration be
    20 KB (3,359 words) - 21:06, 9 March 2017
  • 20.109(S17):Complete Western blot and induce DNA damage for survival and quantitative PCR assays (Day2)
    Researchers often work on multiple experiments at the same time. For this to be fruitful, it is c #*Keep in mind that the washing steps work by dilution, so it is a balance between adding enough to create a sink for
    20 KB (3,175 words) - 01:02, 7 March 2017
  • 20.109(S17):Assess cell survival and harvest RNA for quantitative PCR assay (Day3)
    ...gene expression as DNA molecules are more stable and, therefore, easier to work with in experiments. As with the RNA purification, we will use a kit to ge ...e you begin your work in tissue culture, prepare your laboratory bench for work with RNA. As noted in the introduction, RNA is very sensitive to degradati
    13 KB (2,208 words) - 01:48, 9 February 2017
  • 20.109(S17):Practice data analysis methods (Day6)
    ...ty may not be able to help you with bugs or issues. You are encouraged to work through the exercises while in class on the laboratory computers.
    1 KB (172 words) - 01:35, 3 April 2017
  • 20.109(S17):Examine quantitative PCR results (Day9)
    ...inal laboratory session for Module 2! You have completed all of the bench work for your research; however, there is still data analysis to complete for th
    7 KB (1,142 words) - 15:44, 12 April 2017
  • 20.109(S17):Purify RNA for quantitative PCR assay and treat cells for survival assay (Day3)
    ...gene expression as DNA molecules are more stable and, therefore, easier to work with experimentally. As with the RNA purification, we will use a kit to ge #*Keep in mind that the washing steps work by dilution, so it is a balance between adding enough to create a sink for
    15 KB (2,526 words) - 19:11, 30 October 2017
  • 20.109(F17): Fall 2017 Schedule
    ... at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of
    10 KB (1,618 words) - 20:44, 6 December 2017
  • 20.109(F17):Lab tour
    ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it.
    24 KB (3,974 words) - 13:25, 14 September 2017
  • 20.109(F17): Assignments
    ...nt and in the ways that your learning will be assessed. Although your lab work will be done in pairs, <font color = red>'''most assignments will be submit ...ongly recommend that you plan ahead and and schedule time to complete your work when possible.
    8 KB (1,147 words) - 16:16, 30 November 2017
  • 20.109(F17):Communication
    You will use an online laboratory notebook to document your work in 20.109. [http://benchling.com/ Benchling] is a free program that allows ... elements, including organization, clarity, and proper attribution for the work.
    30 KB (4,511 words) - 15:37, 20 November 2017
  • 20.109(F17):Homework
    ... on, you will draft portions of the Module 1 methods. For this assignment, work with your laboratory partner to draft a methods section that details the pr ... the four potential topic bullets listed under the Implications and Future Work content guidelines.
    21 KB (3,308 words) - 19:34, 27 October 2017
  • 20.109(F17):Prepare comet chips (Day1)
    #*Be careful not to introduce bubbles into the agarose and work quickly as the agarose will solidify as it cools. During your work in tissue culture, you will use a hemocytometer to count mammalian cells.
    18 KB (2,935 words) - 20:42, 19 September 2017
  • 20.109(F17):Develop experiment to optimize loading variables (Day2)
    #*You will need to work quickly from this point as the LMP agarose will solidify as it cools.
    14 KB (2,296 words) - 14:28, 15 September 2017
  • Assignment 1 Overview: Transillumination microscopy
    ...nto 5 assignments. Each assignment requires some problem solving, some lab work, some analysis, lots of clear thinking, and an individually written answer ...erstanding log plots|Read this page]] to remind yourself how log-log plots work.
    5 KB (825 words) - 17:05, 11 February 2020
  • Assignment 2: fluorescence microscopy
    Submit your work on Stellar in a single PDF file with the naming convention <Lastname><First
    3 KB (531 words) - 18:23, 14 February 2020
  • Assignment 2: epi illuminator for fluorescence microscopy
    ... laser with focusing optics. You must attend the safety lecture before you work with the laser. See an instructor if you missed the lecture. Do not begin w When you work with lasers, keep these laser safety best practices in mind:
    8 KB (1,447 words) - 14:06, 25 August 2017
  • Assignment 7 Overview
    Submit your work on Stellar in a single PDF file with the naming convention <Lastname><First
    13 KB (1,864 words) - 21:49, 3 April 2018
  • Assignment 5 Overview
    Submit your work in on Stellar in a single PDF file with the naming convention <Lastname><Fi
    3 KB (426 words) - 13:34, 23 March 2020
  • Assignment 1, Part 2: Optics bootcamp
    * The object needs to be initialized by ''invoking'' its <tt>initalize</tt> method. Do this by ...t>Acquire</tt>, so you have to save the data somewhere else if you want to work with it later on.
    20 KB (3,371 words) - 06:40, 31 January 2020
  • Assignment 1, Part 1: Pre-lab questions
    ...he image plane since there is no place the rays actually come together; it needs another lens (such as your eye) in order to view it. More examples of real ... ray tracing and Snell's law to locate the image of the turtle. Show your work.</li>
    20 KB (3,312 words) - 20:00, 5 September 2019
  • 20.109(F17): M1 Data Summary
    ...in figures and supporting text – including context for understanding the work’s broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    11 KB (1,562 words) - 19:31, 11 October 2017
  • Assignment 1, Part 3: Building your transillumination microscope
    Nice job! Take a minute to admire your work. You and this microscope will be spending a lot of time together this semes
    38 KB (6,482 words) - 19:23, 6 September 2019
  • Assignment 4: finding and measuring things
    ...work on your code in 16-336 during lab hours. The instructors are happy to work alongside you to help you get through MATLAB's many curiosities and frustra
    2 KB (244 words) - 14:49, 17 September 2018
  • Assignment 7, Part 2: measure temperature and fluorescence
    ...as some shortcomings, it will give you a good idea of how all of the parts work together to make melting curves. The instrument includes a resistance tempe ...his lab. You will probably notice some shortcomings your instrument as you work. Don't worry about that &mdash; in part 2, you will make many improvements
    22 KB (3,650 words) - 17:42, 6 April 2018
  • Assignment 3 Overview
    Submit your work on Stellar in a single PDF file with the naming convention <Lastname><First
    3 KB (377 words) - 14:08, 1 October 2018
  • Recording, displaying and saving images in MATLAB
    <li> One way to make this work is to rescale your image to 16 bits: <tt> imshow( 65535/4095 * microruler10 <li> An even better way to work with images in MATLAB is to convert them to [http://en.wikipedia.org/wiki/D
    5 KB (834 words) - 18:41, 31 January 2019
  • Assignment 6 Overview
    ...t 1: Pre-lab questions|A few questions to answer before you start your lab work.]]; Submit your work on Stellar in a single PDF file with the naming convention <Lastname><First
    3 KB (432 words) - 12:51, 14 October 2018
  • Assignment 7, Part 1: op amp golden rules questions
    Now work out the expression for this new circuit's output with respect to the curren
    6 KB (955 words) - 21:44, 3 April 2018
  • Assignment 8 Overview
    Submit your work on Stellar in a single PDF file with the naming convention <Lastname><First ...your apparatus with sufficient detail for another person to replicate your work. Assume the reader is familiar with the concepts of 20.309 and has access t
    4 KB (615 words) - 18:43, 29 January 2018
  • Assignment 9 Overview
    {{Template:Assignment Turn In|message=Turn in all of your work (comprehensive list below) on Stellar in a single PDF file named <lastname>
    6 KB (872 words) - 00:20, 25 January 2018
  • Assignment 9, Part 1: model function
    ...th state variables. Because of this assumption, lumped element models only work well if the bodies you are modeling have a nearly uniform temperature. Comp
    12 KB (1,969 words) - 15:29, 14 November 2017
  • 20.109(F17):Complete in silico cloning (Day1)
    '''Be sure to document your work and answer all questions in your lab notebook as you progress through the e #Be sure to record all of your work for the ligation calculations in your notebook.
    23 KB (3,835 words) - 19:34, 13 October 2017
  • 20.109(F17):Design gRNA for CRISPRi (Day2)
    ...mine the results of your digests and perform the next stage of preparatory work by selecting a target for your metabolism engineering project. Upon determ
    13 KB (2,115 words) - 20:47, 17 October 2017
  • 20.109(F17):Generate gRNA plasmid (Day3)
    ...reaction, to ensure that all the reagents are working properly. You should work quickly but carefully, and keep your tube in a chilled container at all tim
    9 KB (1,451 words) - 16:32, 19 October 2017
  • 20.109(F17):Confirm gRNA sequence (Day5)
    ...ant to remove all traces of ethanol, as they may interfere with subsequent work with the DNA.
    14 KB (2,298 words) - 18:35, 27 October 2017
  • 20.109(F17):Journal club II (Day 6)
    ...s focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. R ...ease email it (as .pdf) to your instructor with a brief description of the work.
    10 KB (1,586 words) - 05:39, 20 October 2017
  • 20.109(F17):Growth of phage materials (Day1)
    ...ientists who can tolerate a mix of p8 proteins on the phage coat for their work, there are phage-display variations that mix and match fusion and wild-type ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (''e.g'' see reference [http://www.sciencemag.org/cgi/content/abstract/312/
    16 KB (2,693 words) - 16:31, 14 November 2017
  • 20.109(F17):Purify active materials (Day2)
    ...isms generate minerals that are often used to harden soft tissues. In our work for module 3 we are using M13 as a template for the biomineralization of ph
    6 KB (1,012 words) - 19:28, 16 November 2017
  • 20.109(F17):Cathode construction (Day3)
    ...ate, which may impede the current flow required for the battery to perform work. ...heir time, equipment, and space. Because you will be completing laboratory work in the Belcher Lab, it is important that you take goggles and a lab coat wi
    10 KB (1,581 words) - 17:02, 22 August 2017
  • 20.109(F17):Visualize active material using TEM (Day4)
    ===How does a TEM work?=== #Use the downtime to work on your [[20.109(F16): Biomaterials engineering mini-report| Biomaterials e
    5 KB (767 words) - 16:53, 22 August 2017
  • 20.109(F17):Research proposal presentation
    * needed resources to complete the work (not a separate slide, integrated when relevant) *describes/credits relevant prior work
    15 KB (2,374 words) - 18:49, 17 November 2017
  • 20.109(F17): Biomaterials engineering mini-report
    ...summarize and interpret the class-wide findings and briefly suggest future work. What we usually call Results and Discussion should be combined. The Method |Contextualizing Results and Suggestions for Future Work
    4 KB (529 words) - 18:53, 17 November 2017
  • 20.109(F17): M1 Mini-presentation
    ...erm "elevator pitch" is used to describe a brief oral presentation of your work; it suggests that you have the time of an elevator ride to explain the impo ...uld both introduce your research project and convey the importance of your work in the context of the field. You want the listener to understand why your
    4 KB (652 words) - 19:30, 6 September 2017
  • 20.109(F17):Evaluate cell loading results (Day3)
    #*Be careful not to introduce bubbles into the agarose and work quickly as the agarose will solidify as it cools.
    8 KB (1,360 words) - 20:41, 19 September 2017
  • 20.109(F17):Test role of biochemical factors in genomic stability (Day4)
    #*You will need to work quickly from this point as the LMP agarose will solidify as it cools. #*Work at the piece of diaper paper located at the front laboratory bench when pre
    12 KB (1,986 words) - 21:55, 21 September 2017
  • 20.109(F17):Complete biochemical experiment (Day5)
    ... excisions, abasic sites, strand breaks, and crosslinks. The experimental work you complete in the next three laboratory classes will focus on a technique ...chemical you used for the biochemical testing experiment. Each group will work with two plates: one will be used to assess the level of damage following t
    12 KB (1,953 words) - 12:41, 29 September 2017
  • 20.109(F17): Research article
    ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion. ...y appropriate citations. '''Any details you found in another researcher's work should be cited.'''
    8 KB (1,240 words) - 16:23, 15 November 2017
  • 20.109(F17):Visualize and analyze data for sub-nuclear foci assay (Day7)
    #*Check a few other files to see if the same threshold values work for them.
    9 KB (1,538 words) - 17:57, 11 October 2017
  • Assignment 2 Part 2: Fluorescence microscopy
    ...e should also consider aberrations of our optical system. Spherical lenses work well under the small angle approximation. For our LED, the light is actuall
    18 KB (2,752 words) - 18:39, 12 September 2019
  • Assignment 2 Part 3: Build an epi-illuminator for your microscope
    ... to cause harm to your eyes. You must attend the safety lecture before you work with high power LEDs in lab. See an instructor if you missed the lecture. D When you work with high power light sources, keep these best practices in mind:
    13 KB (2,282 words) - 19:21, 21 February 2020
  • Assignment 4 part 2: Measure resolution
    ...ear fit. The MATLAB function <tt>nlinfit</tt> will then do the rest of the work for you. It will take your four inputs, calculate the sum of squared residu
    24 KB (3,552 words) - 19:42, 28 February 2020
  • Assignment 4 part 3: Track microspheres over time
    ...iments of J. Perrin that lead to his Nobel Prize. A translation of the key work is included in the reprints folder. Have a look – he used latex spheres, ...nt: you may need to make some small modifications to the code to get it to work. Perhaps the code you wrote in Parts 1 and 2 will be helpful...)
    10 KB (1,344 words) - 19:58, 28 February 2020
  • 20.109(S18): Spring 2018 schedule
    ...habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of th
    12 KB (1,806 words) - 02:26, 14 May 2018
  • 20.109(S18):In silico cloning and confirmation digest of protein expression vector(Day1)
    ...t was cloned by the teaching faculty. In this exercise you will virtually work through the cloning steps that were used to ligate the FKBP12 gene into the '''Be sure to document your work and answer all questions in your lab notebook as you progress through the e
    23 KB (3,774 words) - 21:17, 16 February 2018
  • 20.109(S18):Practice tissue culture techniques and prepare cells for RNA purification (Day1)
    You will work with these cells, mostly, in our tissue culture room. Tissue culture was d As you prepare to work in the tissue culture room, review the following resource:
    13 KB (2,165 words) - 13:44, 20 March 2018
  • 20.109(S18):Analyze small microarray data and induce protein expression (Day2)
    #*Be sure that a cell within your spreadsheet is highlighted (any cell will work).
    17 KB (2,771 words) - 21:15, 16 February 2018
  • 20.109(S18):Laboratory tour
    ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it. ...how your grade will be determined. What is the policy for submitting late work (specifically, how are late assignments graded)?
    26 KB (4,362 words) - 21:39, 6 February 2018
  • 20.109(S18):Evaluate protein purity and concentration (Day4)
    #*During the incubation step, work on Part 4
    12 KB (1,851 words) - 20:02, 26 February 2018
  • 20.109(S18):Journal club presentations (Day6 and 7)
    ...s focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. R ...in only 10 minutes. Therefore, this assignment is not only to present the work, but also to identify the data that is most important to the conclusions.
    15 KB (2,395 words) - 15:10, 28 June 2018
  • 20.109(S18): Biomaterials engineering mini-report
    ...summarize and interpret the class-wide findings and briefly suggest future work. What we usually call Results and Discussion should be combined. The Method |Contextualizing Results and Suggestions for Future Work
    4 KB (541 words) - 15:59, 6 January 2018
  • 20.109(S18): Assignments
    ...in the ways that your learning will be assessed. Although your laboratory work will be done as partners, <font color = #0404B4>'''several assignments will ...ongly recommend that you plan ahead and and schedule time to complete your work when possible.
    12 KB (1,814 words) - 19:30, 31 August 2018
  • 20.109(S18): Research article
    ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion. ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed.
    27 KB (4,131 words) - 18:31, 31 January 2018
  • 20.109(S18):Research proposal presentation
    * needed resources to complete the work (not a separate slide, integrated when relevant) ...n this proposal. For example, if you propose molecular and/or ''in vitro'' work, you would want to be able to say a few words about ''in vivo'' experiments
    12 KB (2,079 words) - 21:19, 10 January 2018
  • 20.109(S18):Growth of phage-based active (cathode) material (Day1)
    ...ientists who can tolerate a mix of p8 proteins on the phage coat for their work, there are phage-display variations that mix and match fusion and wild-type ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (''e.g'' see reference [http://www.sciencemag.org/cgi/content/abstract/312/
    17 KB (2,773 words) - 13:17, 30 April 2018
  • 20.109(S18):Purify phage-based active (cathode) material (Day2)
    ...isms generate minerals that are often used to harden soft tissues. In our work for module 3 we are using M13 as a template for the biomineralization of ph
    6 KB (1,017 words) - 16:41, 24 April 2018
  • 20.109(S18):Construct cathode with phage-based active material (Day3)
    ...ate, which may impede the current flow required for the battery to perform work. ...heir time, equipment, and space. Because you will be completing laboratory work in the Belcher Lab, it is important that you take goggles and a lab coat wi
    10 KB (1,562 words) - 15:43, 27 April 2018
  • 20.109(S18):Visualize phage nanowires using TEM (Day4)
    #Use the downtime to work on your [[20.109(S18): Biomaterials engineering mini-report| Mini-report]]
    5 KB (758 words) - 02:56, 5 January 2018
  • 20.109(S18):Purify RNA and practice RNA-seq data analysis methods (Day3)
    ...gene expression as DNA molecules are more stable and, therefore, easier to work with experimentally. As with the RNA purification, we will use a kit to ge ...etrieve your flasks from tissue culture, prepare your laboratory bench for work with RNA. As noted in the introduction, RNA is very sensitive to degradati
    12 KB (1,963 words) - 00:38, 19 March 2019
  • 20.109(S18):Homework
    ..., you will draft portions of the Module 1 methods. For this assignment, '''work with your partner''' to write sub-sections that detail the protocols you co ...sketched out; you simply have to describe five recent examples of existing work that you find interesting. However, you can start to brainstorm how to expa
    23 KB (3,594 words) - 18:41, 3 May 2018
  • 20.109(S18): M1 Data Summary
    ...in figures and supporting text – including context for understanding the work and insight into the broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    9 KB (1,376 words) - 16:05, 10 March 2018
  • 20.109(S18): M1 Mini-presentation
    ...erm "elevator pitch" is used to describe a brief oral presentation of your work; it suggests that you have the time of an elevator ride to explain the impo ...uld both introduce your research project and convey the importance of your work in the context of the field. You want the listener to understand why your
    4 KB (602 words) - 19:10, 6 January 2018
  • 20.109(S18):Confirm ligand binding using differential scanning fluorimetry assay (Day6)
    #*If this regression does not work well, you may use alternative methods to estimate an apparent K<sub>D</sub>
    11 KB (1,775 words) - 13:16, 8 March 2018
  • 20.109(S18):Complete data analysis (Day7)
    ...inal laboratory session for Module 1! You have completed all of the bench work for your research; however, there is still data analysis to complete for yo
    5 KB (767 words) - 21:44, 25 January 2018
  • 20.109(F18): Fall 2018 schedule
    ...habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of th
    11 KB (1,626 words) - 16:06, 5 December 2018
  • 20.109(F18):Homework
    ... on, you will draft portions of the Module 1 methods. For this assignment, work with your laboratory partner to draft a methods section that details the pr ... the four potential topic bullets listed under the Implications and Future Work content guidelines. This assignment is to be completed individually (not wi
    22 KB (3,505 words) - 21:06, 21 August 2019
  • 20.109(F18): Assignments
    ...in the ways that your learning will be assessed. Although your laboratory work will be done as partners, <font color = #33ff5e>'''several assignments will ...ongly recommend that you plan ahead and and schedule time to complete your work when possible.
    13 KB (1,821 words) - 16:07, 5 December 2018
  • 20.109(F18):Lab tour
    ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it. ...how your grade will be determined. What is the policy for submitting late work (specifically, how are late assignments graded)?
    27 KB (4,435 words) - 19:28, 6 September 2018
  • 20.109(F18): Mini-report
    ...summarize and interpret the class-wide findings and briefly suggest future work. What we usually call Results and Discussion should be combined. The Method |Contextualizing Results and Suggestions for Future Work
    4 KB (543 words) - 19:49, 23 August 2018
  • 20.109(F18):Complete in silico cloning (Day1)
    '''Be sure to document your work and answer all questions in your lab notebook as you progress through the e #Be sure to record all of your work for the ligation calculations in your notebook.
    23 KB (3,819 words) - 13:51, 12 September 2019
  • 20.109(F18):Design gRNA for CRISPRi (Day2)
    ...mine the results of your digests and perform the next stage of preparatory work by selecting a target for your metabolism engineering project. Upon determ
    13 KB (2,112 words) - 15:29, 25 October 2018
  • 20.109(F18):Generate gRNA plasmid (Day3)
    ...reaction, to ensure that all the reagents are working properly. You should work quickly but carefully, and keep your tube in a chilled container at all tim
    9 KB (1,441 words) - 20:11, 15 October 2018
  • 20.109(F18):Journal club presentation (Day4 and 6)
    ...s focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. R ...in only 10 minutes. Therefore, this assignment is not only to present the work, but also to identify the data that is most important to the conclusions.
    12 KB (1,816 words) - 19:15, 23 October 2018
  • 20.109(F18):Confirm gRNA sequence (Day5)
    ...ant to remove all traces of ethanol, as they may interfere with subsequent work with the DNA.
    14 KB (2,323 words) - 18:26, 25 October 2018
  • 20.109(F18):Growth of phage-based active (cathode) material (Day1)
    ...ientists who can tolerate a mix of p8 proteins on the phage coat for their work, there are phage-display variations that mix and match fusion and wild-type ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (''e.g'' see reference [http://www.sciencemag.org/cgi/content/abstract/312/
    16 KB (2,742 words) - 18:15, 13 November 2018
  • 20.109(F18):Purify phage-based active (cathode) material (Day2)
    ...isms generate minerals that are often used to harden soft tissues. In our work for module 3 we are using M13 as a template for the biomineralization of ph
    6 KB (989 words) - 18:13, 13 November 2018
  • 20.109(F18):Construct cathode with phage-based active material (Day3)
    ...ate, which may impede the current flow required for the battery to perform work. ...heir time, equipment, and space. Because you will be completing laboratory work in the Belcher Lab, it is important that you take goggles and a lab coat wi
    10 KB (1,564 words) - 15:47, 28 June 2018
  • 20.109(F18):Visualize phage nanowires using TEM (Day4)
    #Use the downtime to work on your [[20.109(S18): Biomaterials engineering mini-report| Mini-report]]
    5 KB (760 words) - 15:48, 28 June 2018
  • 20.109(F18):Research proposal presentation
    * needed resources to complete the work (not a separate slide, integrated when relevant) ...n this proposal. For example, if you propose molecular and/or ''in vitro'' work, you would want to be able to say a few words about ''in vivo'' experiments
    12 KB (2,081 words) - 19:49, 23 August 2018
  • 20.109(F18): Research article
    ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion. ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed.
    27 KB (4,087 words) - 01:33, 8 November 2018
  • 20.109(F18):Image sub-nuclear foci assay
    #*Check a few other files to see if the same threshold values work for them.
    9 KB (1,538 words) - 15:34, 23 August 2018
  • 20.109(F18):Image sub-nuclear foci assay (Day6)
    #*Check a few other files to see if the same threshold values work for them.
    10 KB (1,563 words) - 15:38, 23 August 2018
  • 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7)
    ...inal laboratory session for Module 1! You have completed all of the bench work for your research; however, there is still data analysis to complete for yo
    16 KB (2,655 words) - 19:34, 4 October 2018
  • 20.109(F18):Practice tissue culture and prepare microwell array (Day1)
    #*Be careful not to introduce bubbles into the agarose and work quickly as the agarose will solidify as it cools. During your work in tissue culture, you will use a hemocytometer to count mammalian cells.
    19 KB (3,096 words) - 18:35, 26 September 2018
  • 20.109(F18):Prepare and treat cells for genomic instability experiment (Day3)
    #*You will need to work quickly from this point as the LMP agarose will solidify as it cools.
    9 KB (1,587 words) - 22:12, 20 September 2018
  • 20.109(F18):Design cell loading optimization experiment and discuss genomic instability experiment (Day2)
    During any downtime, work through Part #4 with your laboratory partner. #*You will need to work quickly from this point as the LMP agarose will solidify as it cools.
    16 KB (2,668 words) - 17:38, 25 September 2018
  • 20.109(F18):Complete genomic instability experiment and load cells for sub-nuclear foci assay (Day4)
    ... excisions, abasic sites, strand breaks, and crosslinks. The experimental work you complete in the next three laboratory classes will focus on a technique
    6 KB (1,038 words) - 15:12, 25 September 2018
  • 20.109(F18): Mini-presentation
    ...erm "elevator pitch" is used to describe a brief oral presentation of your work; it suggests that you have the time of an elevator ride to explain the impo ...uld both introduce your research project and convey the importance of your work in the context of the field. You want the listener to understand why your
    4 KB (604 words) - 20:02, 2 October 2018
  • 20.109(F18): Data Summary
    ...in figures and supporting text – including context for understanding the work and insight into the broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    11 KB (1,571 words) - 20:03, 2 October 2018
  • Assignment 5, Part 1: MSD difference tracking and microscope stability
    ...tion</tt> tool. (Otherwise, you will have a gigantic movie that is hard to work with.)
    9 KB (1,018 words) - 19:38, 1 April 2020
  • Electronics boot camp I: passive circuits and transfer functions
    ...cs written problems II|A few questions to answer before you start your lab work.]]; Submit your work on Stellar in a single PDF file with the naming convention <Lastname><First
    1 KB (167 words) - 17:22, 23 January 2020
  • Assignment 8 Overview: flow channel & two-color microscope
    ...ues used in electronics. In the remaining assignments this semester, we'll work towards measuring the frequency-dependent osmotic shock response of yeast c Submit your work on Stellar in a single PDF file with the naming convention <Lastname><First
    1 KB (194 words) - 15:51, 26 March 2020
  • 20.109(S20):Journal club presentation (Day4 and 6)
    ...s focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. R ...in only 10 minutes. Therefore, this assignment is not only to present the work, but also to identify the data that is most important to the conclusions.
    14 KB (2,050 words) - 20:18, 8 March 2020
  • Assignment 8, Part 2: fabricate a microfluidic device
    The remaining lab assignments will be based on work published in ''Science'' by MIT researchers in 2008 <ref>[http://science.sc ...and sticky. Work over a sheet of aluminum foil rather than directly on the work bench, wear gloves when pouring the elastomer base and crosslinking agent,
    7 KB (1,150 words) - 15:50, 26 March 2020
  • Assignment 8, Part 3: add flow control and test your device
    ...nd off the solenoid valve at a particular rate. Let's get MATLAB to do the work for us by interfacing the valves with a lab computer. While we're at it, wo ...to detail about how these incredibly useful and versatile circuit elements work, but [https://www.youtube.com/watch?v=IcrBqCFLHIY| this video goes through
    14 KB (2,339 words) - 22:11, 7 November 2019
  • Assignment 9 Overview: Analyzing yeast images
    Submit your work on Stellar in a single PDF file with the naming convention <Lastname><First
    3 KB (385 words) - 21:03, 14 November 2019
  • Assignment 9, Part 1: Analyze two-color yeast images
    ...t we want to image cells over long times (minutes to hours). These factors work against us to produce high background levels caused by autofluorescence of As you work through this part of the Assignment, fill out the <tt>AnalyzeFrame</tt> fun
    10 KB (1,426 words) - 16:08, 12 November 2019
  • Assignment 10, Part 1: Measuring the osmotic shock response of yeast
    Assignment 10 is the culmination of a lot of hard work. You've built a two-color epi-fluorescence microscope, including computer c * These experiments are tricky and will only work if you are careful and vigilant. It's tempting to walk away while your data
    16 KB (2,644 words) - 21:39, 21 November 2019
  • 20.109(S19): Spring 2019 schedule
    ...habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of th
    11 KB (1,772 words) - 19:16, 8 May 2019
  • 20.109(S19): M1 Mini-presentation
    ...erm "elevator pitch" is used to describe a brief oral presentation of your work; it suggests that you have the time of an elevator ride to explain the impo ...uld both introduce your research project and convey the importance of your work in the context of the field. You want the listener to understand why your
    4 KB (599 words) - 18:14, 29 January 2019
  • 20.109(S19):Laboratory tour
    ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it. ...how your grade will be determined. What is the policy for submitting late work (specifically, how are late assignments graded)?
    27 KB (4,454 words) - 15:43, 6 February 2019
  • 20.109(S19): Assignments
    ...in the ways that your learning will be assessed. Although your laboratory work will be done as partners, <font color = #0404B4>'''several assignments will ...ongly recommend that you plan ahead and and schedule time to complete your work when possible.
    13 KB (1,840 words) - 20:47, 10 April 2019
  • 20.109(S19):Homework
    ..., you will draft portions of the Module 1 methods. For this assignment, '''work with your partner''' to write sub-sections that detail the protocols you co ...e to four potential topic bullets listed under the Implications and Future Work content guidelines. This assignment is to be completed individually (not wi
    24 KB (3,715 words) - 01:02, 30 April 2019
  • 20.109(S19):Research proposal presentation
    * needed resources to complete the work (not a separate slide, integrated when relevant) ...n this proposal. For example, if you propose molecular and/or ''in vitro'' work, you would want to be able to say a few words about ''in vivo'' experiments
    12 KB (2,079 words) - 19:33, 29 January 2019
  • 20.109(S19): Mini-report
    ...summarize and interpret the class-wide findings and briefly suggest future work. What we usually call Results and Discussion should be combined. The Method |Contextualizing Results and Suggestions for Future Work
    4 KB (542 words) - 19:33, 29 January 2019
  • 20.109(S19):Growth of phage-based active (cathode) material (Day1)
    ...ientists who can tolerate a mix of p8 proteins on the phage coat for their work, there are phage-display variations that mix and match fusion and wild-type ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (''e.g'' see reference [http://www.sciencemag.org/cgi/content/abstract/312/
    16 KB (2,740 words) - 16:36, 18 April 2019
  • 20.109(S19):Purify phage-based active (cathode) material (Day2)
    ...isms generate minerals that are often used to harden soft tissues. In our work for module 3 we are using M13 as a template for the biomineralization of ph
    5 KB (851 words) - 17:00, 24 April 2019
  • 20.109(S19):Construct cathode with phage-based active material (Day3)
    ...ate, which may impede the current flow required for the battery to perform work. ...heir time, equipment, and space. Because you will be completing laboratory work in the Belcher Lab, it is important that you take goggles and a lab coat wi
    10 KB (1,562 words) - 17:59, 29 January 2019
  • 20.109(S19):Visualize phage nanowires using TEM (Day4)
    #Use the downtime to work on your [[20.109(S19): Biomaterials engineering mini-report| Mini-report]]
    5 KB (758 words) - 18:01, 29 January 2019
  • 20.109(S19): Data Summary
    ...in figures and supporting text – including context for understanding the work and insight into the broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    10 KB (1,422 words) - 14:19, 12 March 2019
  • 20.109(S19): Mini-presentation
    ...erm "elevator pitch" is used to describe a brief oral presentation of your work; it suggests that you have the time of an elevator ride to explain the impo ...uld both introduce your research project and convey the importance of your work in the context of the field. You want the listener to understand why your
    4 KB (599 words) - 18:16, 29 January 2019
  • 20.109(S19):Purify RNA and practice RNA-seq data analysis methods (Day3)
    ...gene expression as DNA molecules are more stable and, therefore, easier to work with experimentally. As with the RNA purification, we will use a kit to ge ...ou harvest your cells in tissue culture, prepare your laboratory bench for work with RNA. As noted in the introduction, RNA is very sensitive to degradati
    12 KB (1,923 words) - 20:29, 16 April 2019
  • 20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1)
    ...esearchers in the Koehler Laboratory. In this exercise you will virtually work through the cloning steps that were used to ligate the FKBP12 gene into the <font color = red>'''Be sure to document your work and answer all questions in your lab notebook as you progress through the e
    22 KB (3,675 words) - 19:34, 25 February 2019
  • 20.109(S18):Analyze small microarray data (Day3)
    #*Be sure that a cell within your spreadsheet is highlighted (any cell will work).
    14 KB (2,194 words) - 17:14, 7 March 2019
  • 20.109(S19):Confirm ligand binding using differential scanning fluorimetry assay (Day6)
    #*If this regression does not work well, you may use alternative methods to estimate an apparent K<sub>D</sub>
    10 KB (1,695 words) - 20:09, 5 March 2019
  • 20.109(S19):Complete data analysis (Day7)
    ...inal laboratory session for Module 1! You have completed all of the bench work for your research; however, there is still data analysis to complete for yo
    5 KB (766 words) - 15:55, 31 January 2019
  • 20.109(S19):Practice tissue culture techniques and prepare cells for RNA purification (Day1)
    You will work with these cells, mostly, in our tissue culture room. Tissue culture was d As you prepare to work in the tissue culture room, review the following resource:
    14 KB (2,344 words) - 20:32, 16 April 2019
  • 20.109(S19):Journal club presentations (Day7 and 8)
    ...s focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. R ...in only 10 minutes. Therefore, this assignment is not only to present the work, but also to identify the data that is most important to the conclusions.
    15 KB (2,407 words) - 00:58, 4 April 2019
  • 20.109(S19):Guidelines for working in the tissue culture facility
    ...ut most are common practice and will be good habits for any tissue culture work you do. ...best with the blowers off when not in use. If you are the first person to work in the hood, turn the blower on, open the sash, and wait 10 minutes before
    7 KB (1,195 words) - 23:28, 6 March 2019
  • 20.109(S19): Research article
    ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion. ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed.
    27 KB (4,062 words) - 19:40, 19 April 2019
  • 20.109(S19):Design cell viability experiment and complete RNA-seq data analysis (Day6)
    ...successful experiment requires time, effort, and practice. Today you will work with the Teaching faculty to design an experiment based on the research que In addition, you will work with your partner to write a protocol that lists all of the steps for your
    4 KB (583 words) - 14:46, 2 April 2019
  • 20.109(F19): Fall 2019 schedule
    ...habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of th
    11 KB (1,649 words) - 19:19, 4 December 2019
  • 20.109(F19):Practice cell culture and begin sub-nuclear foci assay (Day1)
    During your work in tissue culture, you will use a hemocytometer to count mammalian cells.
    14 KB (2,233 words) - 16:30, 9 September 2019
  • 20.109(F19):Generate gRNA plasmid (Day3)
    ...reaction, to ensure that all the reagents are working properly. You should work quickly but carefully, and keep your tube in a chilled container at all tim
    9 KB (1,444 words) - 20:44, 18 September 2019
  • 20.109(F19):Lab tour
    ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it. ...how your grade will be determined. What is the policy for submitting late work (specifically, how are late assignments graded)?
    27 KB (4,413 words) - 20:37, 30 August 2019
  • 20.109(F19):Complete in silico cloning (Day1)
    '''Be sure to document your work and answer all questions in your lab notebook as you progress through the e #Be sure to record all of your work for the ligation calculations in your notebook.
    23 KB (3,785 words) - 22:22, 7 August 2019
  • 20.109(F19):Guidelines for working in the tissue culture facility
    ...ut most are common practice and will be good habits for any tissue culture work you do. ...best with the blowers off when not in use. If you are the first person to work in the hood, turn the blower on, open the sash, and wait 10 minutes before
    7 KB (1,197 words) - 18:28, 14 August 2019
  • 20.109(F19):Design gRNA for CRISPRi (Day2)
    ...mine the results of your digests and perform the next stage of preparatory work by selecting a target for your metabolism engineering project. Upon determ
    13 KB (2,121 words) - 13:56, 13 September 2019
  • 20.109(F19):Treat cells for gamma-H2AX (Day2)
    #*Be careful not to introduce bubbles into the agarose and work quickly as the agarose will solidify as it cools.
    10 KB (1,755 words) - 19:12, 17 September 2019
  • 20.109(F19):Complete in silico cloning of pdCas9 (Day1)
    '''Be sure to document your work and answer all questions in your lab notebook as you progress through the e #Be sure to record all of your work for the ligation calculations in your notebook.
    25 KB (4,089 words) - 12:11, 8 October 2019
  • 20.109(F19):Design gRNA sequence for CRISPRi (Day2)
    ...mine the results of your digests and perform the next stage of preparatory work by selecting a target for your metabolism engineering project. Upon determ
    13 KB (2,116 words) - 18:52, 14 August 2019
  • 20.109(F19):Journal club presentation (Day4 and 6)
    ...s focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. R ...in only 10 minutes. Therefore, this assignment is not only to present the work, but also to identify the data that is most important to the conclusions.
    12 KB (1,792 words) - 20:23, 22 October 2019
  • 20.109(F19):Confirm gRNA sequence in pgRNA(Day5)
    ...ant to remove all traces of ethanol, as they may interfere with subsequent work with the DNA.
    14 KB (2,323 words) - 19:11, 14 August 2019
  • 20.109(F19):Confirm gRNA sequence in pgRNA (Day5)
    ...ant to remove all traces of ethanol, as they may interfere with subsequent work with the DNA.
    15 KB (2,355 words) - 17:02, 25 October 2019
  • 20.109(F19):Homework
    ... on, you will draft portions of the Module 1 methods. For this assignment, work with your laboratory partner to draft a methods section that details the pr ... the four potential topic bullets listed under the Implications and Future Work content guidelines. Please still consider the overall organization of the s
    22 KB (3,438 words) - 17:37, 16 October 2019
  • 20.109(F19): Assignments
    ...in the ways that your learning will be assessed. Although your laboratory work will be done as partners, <font color = #CC0000>'''several assignments will ...ongly recommend that you plan ahead and and schedule time to complete your work when possible.
    13 KB (1,813 words) - 16:14, 16 October 2019
  • 20.109(F19):Module 3
    In your experiment, you build upon work completed by students in the Sp17, Sp18, and Sp19 semesters! An SMM was co
    3 KB (437 words) - 18:21, 30 August 2019
  • 20.109(F19):Research proposal presentation
    * needed resources to complete the work (not a separate slide, integrated when relevant) ...n this proposal. For example, if you propose molecular and/or ''in vitro'' work, you would want to be able to say a few words about ''in vivo'' experiments
    12 KB (2,081 words) - 19:26, 5 November 2019
  • 20.109(F19): Research article
    ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion. ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed.
    27 KB (4,080 words) - 23:37, 11 November 2019
  • 20.109(F19):Complete gamma-H2AX assay and prepare CometChip (Day3)
    #*You will need to work quickly from this point as the LMP agarose will solidify as it cools.
    16 KB (2,626 words) - 20:47, 25 September 2019
  • 20.109(F19):Load cells into CometChip and apply treatments (Day4)
    #*You will need to work quickly from this point as the LMP agarose will solidify as it cools. #*Be very careful with MMS and work on the absorbent paper at all times![[File:Fa19 20109 cometchip treatment m
    12 KB (1,976 words) - 15:14, 24 September 2019
  • 20.109(F19):Practice statistical analysis methods and complete data analysis (Day7)
    ...inal laboratory session for Module 1! You have completed all of the bench work for your research; however, there is still data analysis to complete for yo
    7 KB (1,189 words) - 19:54, 2 October 2019
  • 20.109(F19): Mini-presentation
    ...erm "elevator pitch" is used to describe a brief oral presentation of your work; it suggests that you have the time of an elevator ride to explain the impo ...uld both introduce your research project and convey the importance of your work in the context of the field. You want the listener to understand why your
    4 KB (625 words) - 13:07, 19 July 2020
  • Laser cutter safe operating procedure
    * '''Remain at the cutter and keep an attentive eye on the work for the entire duration of the machining operation.''' No exceptions. # Make sure the lens is clean. Notify an instructor if it needs attention.
    9 KB (1,503 words) - 19:03, 15 December 2019
  • 20.109(F19): Data Summary
    ...in figures and supporting text – including context for understanding the work and insight into the broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    10 KB (1,466 words) - 18:34, 23 October 2019
  • 20.109(F19):Prepare for cellular thermal shift assay (Day1)
    This module is a continuation of work started by previous 109ers! Upon identifying putative ligand binders using ... ligand you want to test. Why do you think this ligand will bind FKBP12? Work with your laboratory partner to develop a hypothesis.
    14 KB (2,315 words) - 16:18, 7 November 2019
  • 20.109(F19):Complete antibody staining for Western blot analysis (Day4)
    ...neral approach to the problem. Appropriate justification for the proposed work can be provided through literature citations, data from other sources, or f
    8 KB (1,322 words) - 22:58, 9 September 2019
  • 20.109(F19): Mini-report
    ...summarize and interpret the class-wide findings and briefly suggest future work. What was separated as Results and Discussion in the Research article shoul |Contextualizing Results and Suggestions for Future Work
    4 KB (563 words) - 18:12, 3 December 2019
  • 20.109(S20): Spring 2020 schedule
    ...habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of th
    17 KB (2,729 words) - 21:02, 6 May 2020
  • Assignment 6 Overview: two color microscope
    Submit your work on Stellar in a single PDF file with the naming convention <Lastname><First
    3 KB (480 words) - 01:11, 9 April 2020
  • 20.109(S20):Laboratory tour
    Before you begin work in the lab, please put on the proper PPE. ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it.
    27 KB (4,434 words) - 14:55, 5 February 2020
  • 20.109(S20): Assignments
    ...in the ways that your learning will be assessed. Although your laboratory work will be done as partners, <font color = #CC0000>'''several assignments will ...ongly recommend that you plan ahead and and schedule time to complete your work when possible.
    13 KB (1,823 words) - 14:33, 18 March 2020
  • 20.109(S20):Homework
    ...s of completion or your training record which can be found at "My Training Needs" on Atlas. To access, go to atlas.mit.edu then click the Learning Center t ..., you will draft portions of the Module 1 methods. For this assignment, '''work with your partner''' to write sub-sections that detail the protocols you co
    24 KB (3,726 words) - 16:31, 25 April 2020
  • 20.109(S20):Identify clones to characterize (Day3)
    ...neral approach to the problem. Appropriate justification for the proposed work can be provided through literature citations, data from other sources, or f
    14 KB (2,253 words) - 14:41, 21 April 2020
  • 20.109(S20): Data Summary
    ...in figures and supporting text – including context for understanding the work and insight into the broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    9 KB (1,342 words) - 20:05, 20 March 2020
  • 20.109(S20):Complete in silico cloning and induce TDP43 protein expression (Day1)
    <font color=#B40404>'''Be sure to document your work and answer all questions in your lab notebook as you progress through the e #Be sure to record all of your work for the ligation calculations in your notebook.
    26 KB (4,314 words) - 18:17, 7 February 2020
  • 20.109(S20):Prepare cells for RNA purification and practice RNA-seq data analysis methods (Day1)
    You will work with these cells, mostly, in our tissue culture room. Tissue culture was d As you prepare to work in the tissue culture room, review the following resource:
    13 KB (2,111 words) - 21:39, 1 March 2020
  • 20.109(S20):Purify RNA from etoposide-treated cells and and generate cDNA (Day3)
    ...gene expression as DNA molecules are more stable and, therefore, easier to work with experimentally. As with the RNA purification, we will use a kit to ge ...ou harvest your cells in tissue culture, prepare your laboratory bench for work with RNA. As noted in the introduction, RNA is very sensitive to degradati
    10 KB (1,734 words) - 20:59, 28 February 2020
  • 20.109(S20):Scan SMM slides to identify binders of TDP43 protein (Day5)
    ...h! The questions that you address in your future works experiments should work toward a complete and coherent story.
    5 KB (831 words) - 21:04, 21 February 2020
  • 20.109(S20):Examine TDP43 binders for chemical features (Day7)
    ...re, mechanics, metabolism, and signaling. In these roles, proteins do not work in a vacuum; rather the biological roles of proteins are dependent on direc ...easures the capacity of a thermodynamic system to do maximum or reversible work at constant temperature and pressure. When at equilibrium with constant te
    5 KB (754 words) - 19:01, 2 March 2020
  • 20.109(S20):Journal club presentations (Day4 and 5)
    For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. R ...reen' and select your Journal club presentation slide deck (note: the file needs to be open on your computer), then click 'Share'.
    18 KB (2,824 words) - 21:51, 7 April 2020
  • 20.109(S20): Mini-presentation
    ...erm "elevator pitch" is used to describe a brief oral presentation of your work; it suggests that you have the time of an elevator ride to explain the impo ...uld both introduce your research project and convey the importance of your work in the context of the field. You want the listener to understand why your
    4 KB (626 words) - 16:15, 31 January 2020
  • 20.109(S20):Analyze SMM data (Day6)
    '''You will work with your partner to complete the data analysis using a laboratory computer ...ovides details on what the code below (in boxes) is accomplishing. As you work through the data analysis, make notes in your laboratory notebook regarding
    4 KB (649 words) - 20:02, 26 February 2020
  • 20.109(S20):Analyze RNA-seq data and select gene targets for quantitative PCR (qPCR) experiment (Day 6)
    ...igle, Anne Shen & Prof. Ernest Fraenkel. In this exercise, you will first work through a refresher focused on the clustering methods used in the previous
    6 KB (992 words) - 12:27, 2 April 2020
  • 20.109(S20):Examine qPCR results and complete data analysis (Day8)
    ...ow you will discuss and interpret the data in your Research article, let's work though the below figure:
    5 KB (856 words) - 18:14, 28 March 2020
  • 20.109(S20):Harvest candidate clones (Day2)
    ... coli'' plasmid purification method called alkaline lysis. For yeast there needs to be one additional step to help break down the cell wall, but overall goa To harvest plasmid DNA from yeast we need to do a bit more work to break down the cell wall. Yesterday the 2x10<sup>7</sup> yeast cells wer
    13 KB (2,077 words) - 13:29, 16 April 2020
  • 20.109(S20):Research proposal presentation
    * needed resources to complete the work (not a separate slide, integrated when relevant) ...n this proposal. For example, if you propose molecular and/or ''in vitro'' work, you would want to be able to say a few words about ''in vivo'' experiments
    12 KB (2,106 words) - 15:56, 26 March 2020
  • 20.109(S20): Mini-report
    ...ssignment, summarize and interpret the findings and briefly suggest future work. What was separated as Results and Discussion in the Research article shoul |Contextualizing Results and Suggestions for Future Work
    4 KB (575 words) - 15:53, 21 April 2020
  • 20.109(S20):Guidelines for working in the tissue culture facility
    ...ut most are common practice and will be good habits for any tissue culture work you do. ...best with the blowers off when not in use. If you are the first person to work in the hood, turn the blower on, open the sash, and wait 10 minutes before
    7 KB (1,195 words) - 20:10, 28 February 2020
  • 20.109(S20): Research article
    ...ignment for Module 2 will be a research article in which you describe your work in a written communication format similar to a published journal article. T ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    27 KB (4,110 words) - 16:15, 8 April 2020
  • Spring 2020 assignment 10
    As you work through this part of the Assignment, fill out the <tt>AnalyzeFrame</tt> fun
    8 KB (1,160 words) - 18:43, 26 April 2020
  • 20.109(F20): Fall 2020 schedule
    ...habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of th
    22 KB (3,694 words) - 15:57, 3 December 2020
  • 20.109(F20):Lab tour
    ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it. ...azardous materials. At MIT, all materials used in performing experimental work should be disposed of in biohazard boxes as it is assumed that laboratory c
    27 KB (4,406 words) - 17:48, 31 August 2020
  • 20.109(F20): Assignments
    ...in the ways that your learning will be assessed. Although your laboratory work will be done as partners, <font color = #CC0000>'''several assignments will ...ongly recommend that you plan ahead and and schedule time to complete your work when possible.
    13 KB (1,850 words) - 15:48, 2 October 2020
  • 20.109(F20):M1D1
    During your work in tissue culture, you will use a hemocytometer to count mammalian cells.
    10 KB (1,598 words) - 23:22, 1 September 2020
  • 20.109(F20):Guidelines for working in the tissue culture facility
    ...ut most are common practice and will be good habits for any tissue culture work you do. ...best with the blowers off when not in use. If you are the first person to work in the hood, turn the blower on, open the sash, and wait 10 minutes before
    7 KB (1,196 words) - 01:51, 15 July 2020
  • 20.109(F20):M1D5
    #*You will need to work quickly from this point as the LMP agarose will solidify as it cools.
    15 KB (2,489 words) - 22:13, 17 September 2020
  • 20.109(F20):Module 1
    ..., which poses the potential for enhanced health risks if certain compounds work synergistically in generating DNA lesions. While there are published report
    5 KB (836 words) - 23:39, 28 July 2020
  • 20.109(F20):M1D7
    ...inal laboratory session for Module 1! You have completed all of the bench work for your research; however, there is still data analysis to complete for yo
    8 KB (1,235 words) - 15:55, 24 September 2020
  • 20.109(F20):M2D1
    #*Feel free to take a picture of your hand-written work and embed the image in your notebook.
    25 KB (4,057 words) - 18:52, 15 October 2021
  • 20.109(F20):M2D6
    ...ovides details on what the code below (in boxes) is accomplishing. As you work through the data analysis, make notes in your laboratory notebook regarding
    6 KB (963 words) - 18:15, 27 October 2020
  • 20.109(F20):Mini-presentation
    ...erm "elevator pitch" is used to describe a brief oral presentation of your work; it suggests that you have the time of an elevator ride to explain the impo ...uld both introduce your research project and convey the importance of your work in the context of the field. You want the listener to understand why your
    4 KB (624 words) - 19:24, 25 July 2020
  • 20.109(F20):M3D5
    ...al error and reagent failure, it often happens that an experiment does not work as expected. By including controls, researchers are able to identify these
    8 KB (1,355 words) - 00:08, 31 July 2020
  • 20.109(F20):Module 3
    '''Measure:''' It is not enough to just do an experiment. For the work to be meaningful, the data need to be collected and analyzed. The methods
    4 KB (632 words) - 00:07, 28 July 2020
  • 20.109(F20):M3D2
    ...Media:GRNA Design Worksheet.pdf |here]]). It may be helpful to review the work you completed in Part 2 of the previous laboratory session.
    17 KB (2,660 words) - 23:58, 30 July 2020
  • 20.109(F20):Data Summary
    ...in figures and supporting text – including context for understanding the work and insight into the broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    9 KB (1,364 words) - 19:21, 25 July 2020
  • 20.109(F20):Journal club presentation
    ...s focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. R ...in only 10 minutes. Therefore, this assignment is not only to present the work, but also to identify the data that is most important to the conclusions.
    14 KB (1,998 words) - 16:58, 6 October 2020
  • 20.109(F20):Research proposal presentation
    ... paper for honing in on research gaps! But don't expect them to do all the work for you. The conversation will be more effective if you do some research in ...sion section carefully. What do the authors suggest with respect to future work?
    11 KB (1,760 words) - 19:35, 27 July 2020
  • 20.109(F20):Mini-report
    ...at are necessary to understand the data shown. Finally, explain the future work. |Contextualizing Results and Suggestions for Future Work
    4 KB (578 words) - 18:10, 6 December 2020
  • 20.109(F20):M3D4
    ...neral approach to the problem. Appropriate justification for the proposed work can be provided through literature citations, data from other sources, or f ...spect of this assignment is defining the societal impacts of your proposed work. In recent years, funding agencies have placed increased emphasis on socie
    16 KB (2,633 words) - 15:58, 4 December 2020
  • 20.109(F20):Homework
    ...do not expire and will be valid when are able to return to campus for UROP work! ...s of completion or your training record which can be found at "My Training Needs" on Atlas. To access, go to atlas.mit.edu then click the Learning Center t
    21 KB (3,359 words) - 19:41, 21 September 2020
  • 20.109(F20):M2D7
    ...re, mechanics, metabolism, and signaling. In these roles, proteins do not work in a vacuum; rather the biological roles of proteins are dependent on direc ...easures the capacity of a thermodynamic system to do maximum or reversible work at constant temperature and pressure. When at equilibrium with constant te
    7 KB (1,049 words) - 21:02, 29 October 2020
  • 20.109(F20):Research article
    ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion. ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed.
    27 KB (4,067 words) - 18:54, 25 July 2020
  • In silico cloning of pdCas9 construct
    The goal for this laboratory exercise is to work through the steps that are used to clone a gene of interest into an express #Be sure to record all of your work for the ligation calculations in your notebook.
    19 KB (3,021 words) - 20:40, 3 August 2020
  • 20.109(S21):Spring 2021 schedule
    ...habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of th
    11 KB (1,796 words) - 16:40, 12 May 2021
  • 20.109(S21):M1D3
    To harvest plasmid DNA from yeast we need to do a bit more work to break down the cell wall. Yesterday the 2x10<sup>7</sup> yeast cells wer
    11 KB (1,745 words) - 15:37, 1 March 2021
  • 20.109(S21):M1D4
    ...ion method called alkaline lysis. In the last lab section, for yeast there needs to be one additional step to help break down the cell wall, but overall goa ...e several more washes to purify the plasmid DNA but the major purification work, separating the plasmid from the chromosomal DNA and cell lysate, is comple
    16 KB (2,486 words) - 14:37, 3 March 2021
  • 20.109(S21):Assignments
    ...in the ways that your learning will be assessed. Although your laboratory work will be done as partners, <font color = #CC0000>'''several assignments will ...ongly recommend that you plan ahead and and schedule time to complete your work when possible.
    14 KB (2,009 words) - 21:40, 18 February 2021
  • 20.109(S21):Laboratory tour
    ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it. ...azardous materials. At MIT, all materials used in performing experimental work should be disposed of in biohazard boxes as it is assumed that laboratory c
    28 KB (4,443 words) - 16:02, 16 February 2021
  • 20.109(S21):M2D5
    During your work in tissue culture, you will use a hemocytometer to count mammalian cells. To address these questions, you will work with your laboratory partner. The following resources may be useful to you
    11 KB (1,878 words) - 23:14, 27 April 2021
  • 20.109(S21):M2D4
    ...ovides details on what the code below (in boxes) is accomplishing. As you work through the data analysis, make notes in your laboratory notebook regarding ...re, mechanics, metabolism, and signaling. In these roles, proteins do not work in a vacuum; rather the biological roles of proteins are dependent on direc
    12 KB (1,974 words) - 19:50, 31 March 2021
  • 20.109(S21):Guidelines for working in the tissue culture facility
    ...ut most are common practice and will be good habits for any tissue culture work you do. ...best with the blowers off when not in use. If you are the first person to work in the hood, turn the blower on, open the sash, and wait 10 minutes before
    7 KB (1,197 words) - 22:33, 21 January 2021
  • 20.109(S21):Homework
    ...do not expire and will be valid when are able to return to campus for UROP work! ...s of completion or your training record which can be found at "My Training Needs" on Atlas. To access, go to atlas.mit.edu then click the Learning Center t
    22 KB (3,518 words) - 17:12, 22 April 2021
  • 20.109(S21):M2D6
    **Copy and paste the small molecule smiles into the work space to get a chemical structure
    16 KB (2,706 words) - 21:00, 22 April 2021
  • 20.109(S21):Mini-presentation
    ...erm "elevator pitch" is used to describe a brief oral presentation of your work; it suggests that you have the time of an elevator ride to explain the impo ...uld both introduce your research project and convey the importance of your work in the context of the field. You want the listener to understand why your
    5 KB (694 words) - 22:16, 1 February 2021
  • 20.109(S21):Data Summary
    ...in figures and supporting text – including context for understanding the work and insight into the broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    10 KB (1,476 words) - 03:01, 16 March 2021
  • 20.109(S21):M3D3
    #Work from reservoirs #2 to #12 (lowest calcium concentration to highest calcium
    18 KB (3,041 words) - 15:12, 4 May 2021
  • 20.109(S21):Research article
    ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion. ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed.
    27 KB (4,070 words) - 13:25, 27 January 2021
  • 20.109(S21):Journal club presentation
    ...s focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. R ...in only 10 minutes. Therefore, this assignment is not only to present the work, but also to identify the data that is most important to the conclusions.
    15 KB (2,231 words) - 01:11, 6 April 2021
  • 20.109(S21):Research proposal presentation
    ... paper for honing in on research gaps! But don't expect them to do all the work for you. The conversation will be more effective if you do some research in ...sion section carefully. What do the authors suggest with respect to future work?
    11 KB (1,758 words) - 13:40, 27 January 2021
  • 20.109(S21):Mini-report
    ...at are necessary to understand the data shown. Finally, explain the future work. |Contextualizing Results and Suggestions for Future Work
    4 KB (636 words) - 16:17, 13 May 2021
  • 20.109(F21):Module 2
    The protein you will work with was identified via this process. Though much remains to be learned abo
    5 KB (683 words) - 03:26, 31 October 2021
  • 20.109(F21):Assignments
    ...in the ways that your learning will be assessed. Although your laboratory work will be done as partners, <font color = #CC0000>'''several assignments will ...ongly recommend that you plan ahead and and schedule time to complete your work when possible.
    13 KB (1,949 words) - 18:33, 1 October 2021
  • 20.109(S21):M3D5
    ...reaction, to ensure that all the reagents are working properly. You should work quickly but carefully, and keep your tube in a chilled container at all tim
    10 KB (1,584 words) - 00:50, 3 February 2021
  • 20.109(S21):M3D4
    ...al error and reagent failure, it often happens that an experiment does not work as expected. By including controls, researchers are able to identify these ...re you ready to prepare this assignment in the next laboratory session, '''work with your laboratory partner(s)''' to draft an outline of your Mini-report.
    11 KB (1,857 words) - 18:30, 11 May 2021
  • 20.109(F21):Fall 2021 schedule
    ...habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of th
    11 KB (1,750 words) - 21:15, 1 December 2021
  • 20.109(F21):Module 1
    ..., which poses the potential for enhanced health risks if certain compounds work synergistically in generating DNA lesions. While there are published report
    5 KB (788 words) - 16:19, 3 September 2021
  • Develop Research proposal ideas and presentations
    ...f your team to discuss your projects and get feedback. Afterward you will work with your partner(s) to consider the societal impact of your research proje ...neral approach to the problem. Appropriate justification for the proposed work can be provided through literature citations, data from other sources, or f
    6 KB (863 words) - 16:48, 26 April 2021
  • 20.109(F21):Homework
    ...s of completion or your training record which can be found at "My Training Needs" on Atlas. To access, go to atlas.mit.edu then click the Learning Center t ...rding how experiments were performed. To practice this skill, you will '''work with your laboratory partner''' to draft a methods section that details the
    36 KB (5,868 words) - 18:44, 3 November 2021
  • 20.109(F21):Develop Research proposal ideas and draft presentation
    ...s your research question / goal. To get you started on this task you will work with your co-investigator to draft the aims and objectives for your project ...spect of this assignment is defining the societal impacts of your proposed work. In recent years, funding agencies have placed increased emphasis on socie
    3 KB (533 words) - 19:16, 9 August 2021
  • 20.109(F21):Participate in Research proposal peer reviews
    ...neral approach to the problem. Appropriate justification for the proposed work can be provided through literature citations, data from other sources, or f ...elop new plans to accomplish a scientific goal. In this exercise you will work with your co-investigator to consider alternative approaches for your Resea
    4 KB (652 words) - 18:28, 9 August 2021
  • 20.109(F21):M1D1
    During your work in tissue culture, you will use a hemocytometer to count mammalian cells.
    11 KB (1,852 words) - 14:54, 4 August 2021
  • 20.109(F21):Laboratory tour
    ...with the space and equipment in the teaching laboratory. In this, you will work in partners to complete the Stations described below. At some stations the ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it.
    34 KB (5,598 words) - 20:50, 9 September 2021
  • 20.109(F21):M2D1
    **Copy and paste the small molecule smiles into the work space to get a chemical structure
    17 KB (2,791 words) - 20:00, 15 November 2021
  • 20.109(F21):M2D2
    #*Feel free to take a picture of your hand-written work and embed the image in your notebook.
    21 KB (3,498 words) - 19:42, 15 October 2021
  • 20.109(F21):M1D7
    ...inal laboratory session for Module 1! You have completed all of the bench work for your research; however, there is still data analysis to complete for yo
    8 KB (1,227 words) - 18:35, 6 October 2021
  • 20.109(F21):Data Summary
    ...in figures and supporting text – including context for understanding the work and insight into the broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    9 KB (1,364 words) - 00:24, 7 August 2021
  • 20.109(F21):Research talk
    ...erm "elevator pitch" is used to describe a brief oral presentation of your work; it suggests that you have the time of an elevator ride to explain the impo ...uld both introduce your research project and convey the importance of your work in the context of the field. You want the listener to understand why your
    5 KB (691 words) - 00:24, 8 August 2021
  • 20.109(F21):Journal club presentation
    ...s focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. R ...in only 10 minutes. Therefore, this assignment is not only to present the work, but also to identify the data that is most important to the conclusions.
    14 KB (2,095 words) - 03:04, 15 October 2021
  • 20.109(F21):Research proposal presentation
    ... paper for honing in on research gaps! But don't expect them to do all the work for you. The conversation will be more effective if you do some research in ...sion section carefully. What do the authors suggest with respect to future work?
    11 KB (1,781 words) - 16:41, 28 July 2021
  • 20.109(F21):Research article
    ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion. ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed.
    27 KB (4,141 words) - 13:24, 8 August 2021
  • 20.109(F21):Guidelines for working in the tissue culture facility
    ...ut most are common practice and will be good habits for any tissue culture work you do. ...best with the blowers off when not in use. If you are the first person to work in the hood, turn the blower on, open the sash, and wait 10 minutes before
    7 KB (1,194 words) - 20:24, 3 August 2021
  • 20.109(F21):M1D4
    #*You will need to work quickly from this point as the LMP agarose will solidify as it cools.
    15 KB (2,480 words) - 15:38, 23 September 2021
  • 20.109(F21):Draft research question for major assignment
    ...0.109 is the Research proposal presentation. For this assignment you will work with your laboratory partner, or co-investigator, to develop a novel resear
    2 KB (306 words) - 20:03, 9 August 2021
  • 20.109(S22):Spring 2022 schedule
    ...habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of th
    12 KB (1,899 words) - 16:41, 4 May 2022
  • 20.109(S22):Laboratory tour
    ...with the space and equipment in the teaching laboratory. In this, you will work in partners to complete the Stations described below. At some stations the ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it.
    34 KB (5,639 words) - 21:02, 1 February 2022
  • 20.109(S22):Assignments
    ...in the ways that your learning will be assessed. Although your laboratory work will be done as partners, <font color = #CC0000>'''several assignments will ...ongly recommend that you plan ahead and and schedule time to complete your work when possible.
    13 KB (1,954 words) - 15:55, 28 April 2022
  • 20.109(S22):Homework
    ...s of completion or your training record which can be found at "My Training Needs" on Atlas. To access, go to atlas.mit.edu then click the Learning Center t ...rding how experiments were performed. To practice this skill, you will '''work with your laboratory partner''' to draft a methods section that details the
    36 KB (5,856 words) - 20:07, 15 March 2022
  • 20.109(S22):M2D8
    ...ent! For the rest of class, the Instructors will host office hours as you work in case you have any questions.
    3 KB (404 words) - 00:36, 12 April 2022
  • 20.109(S22):M1D2
    **Copy and paste the small molecule smiles into the work space to get a chemical structure
    19 KB (3,104 words) - 18:31, 4 February 2022
  • 20.109(S22):M1D6
    During your work in tissue culture, you will use a hemocytometer to count mammalian cells. To address these questions, you will work with your laboratory partner. The following resources may be useful to you
    11 KB (1,839 words) - 17:55, 24 February 2022
  • 20.109(S22):M1D7
    ... to developing your scientific communication skills. For this homework, '''work with your laboratory partner''' to incorporate the feedback you received to
    13 KB (2,069 words) - 18:57, 1 March 2022
  • 20.109(S22):M3D1
    ...0.109 is the Research proposal presentation. For this assignment you will work with your laboratory partner, or co-investigator, to develop a novel resear
    2 KB (334 words) - 18:50, 14 January 2022
  • 20.109(S22):M3D2
    ...s your research question / goal. To get you started on this task you will work with your co-investigator to draft the aims and objectives for your project ...elop new plans to accomplish a scientific goal. In this exercise you will work with your co-investigator to consider alternative approaches for your Resea
    3 KB (404 words) - 18:50, 14 January 2022
  • 20.109(S22):M3D3
    ...neral approach to the problem. Appropriate justification for the proposed work can be provided through literature citations, data from other sources, or f ...spect of this assignment is defining the societal impacts of your proposed work. In recent years, funding agencies have placed increased emphasis on socie
    5 KB (796 words) - 18:51, 14 January 2022
  • 20.109(S22):M1D8
    ...inal laboratory session for Module 1! You have completed all of the bench work for your research; however, there is still data analysis to complete for yo
    17 KB (2,800 words) - 19:12, 3 March 2022
  • 20.109(S22):M2D1
    #*Feel free to take a picture of your hand-written work and embed the image in your notebook.
    26 KB (4,242 words) - 14:38, 7 March 2022
  • 20.109(S22):Module 3
    '''Measure:''' It is not enough to just do an experiment. For the work to be meaningful, the data need to be collected and analyzed. The methods
    3 KB (450 words) - 17:34, 19 April 2022
  • 20.109(S22):Research talk
    ...erm "elevator pitch" is used to describe a brief oral presentation of your work; it suggests that you have the time of an elevator ride to explain the impo ...uld both introduce your research project and convey the importance of your work in the context of the field. You want the listener to understand why your
    5 KB (691 words) - 20:35, 18 January 2022
  • 20.109(S22):Research proposal presentation
    ... paper for honing in on research gaps! But don't expect them to do all the work for you. The conversation will be more effective if you do some research in ...sion section carefully. What do the authors suggest with respect to future work?
    11 KB (1,781 words) - 21:21, 18 January 2022
  • 20.109(S22):Research article
    ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion. ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed.
    27 KB (4,142 words) - 21:21, 18 January 2022
  • 20.109(S22):Data Summary
    ...in figures and supporting text – including context for understanding the work and insight into the broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.
    9 KB (1,288 words) - 18:50, 4 February 2022
  • 20.109(S22):M2D3
    ...reaction, to ensure that all the reagents are working properly. You should work quickly but carefully, and keep your tube in a chilled container at all tim
    11 KB (1,801 words) - 19:55, 15 March 2022
  • 20.109(S22):M2D4
    ...ant to remove all traces of ethanol, as they may interfere with subsequent work with the DNA.
    15 KB (2,385 words) - 21:09, 24 January 2022
  • 20.109(S22):Journal club presentation
    ...s focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. R ...in only 10 minutes. Therefore, this assignment is not only to present the work, but also to identify the data that is most important to the conclusions.
    14 KB (2,069 words) - 22:36, 11 March 2022
  • 20.109(F22):Fall 2022 schedule
    ...habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of th
    11 KB (1,709 words) - 17:51, 2 December 2022
  • 20.109(F22):M3D1
    ...0.109 is the Research proposal presentation. For this assignment you will work with your laboratory partner, or co-investigator, to develop a novel resear
    2 KB (330 words) - 19:36, 21 November 2022
  • 20.109(F22):Assignments
    ...in the ways that your learning will be assessed. Although your laboratory work will be done as partners, <font color = #CC0000>'''several assignments will ...ongly recommend that you plan ahead and and schedule time to complete your work when possible.
    13 KB (1,936 words) - 15:20, 23 September 2022

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