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  1. Lab Manual: Atomic Force Microscopy (AFM)‏‎ (421,388 views)
  2. 20.309 Main Page‏‎ (320,463 views)
  3. DNA Melting Part 1: Measuring Temperature and Fluorescence‏‎ (290,927 views)
  4. Main Page‏‎ (287,565 views)
  5. Electronics primer‏‎ (245,054 views)
  6. Electronics Mini-Lab‏‎ (239,259 views)
  7. Geometrical optics and ray tracing‏‎ (204,591 views)
  8. Lab Manual: Optical Microscopy‏‎ (148,108 views)
  9. Assignment 2 Part 1: Noise in images‏‎ (144,195 views)
  10. 20.345:Course Information‏‎ (141,768 views)
  11. Optics Bootcamp‏‎ (124,014 views)
  12. 20.309:Course Information‏‎ (119,230 views)
  13. DNA Melting: Simulating DNA Melting - Intermediate Topics‏‎ (114,395 views)
  14. DNA Melting Part 2: Lock-in Amplifier and Temperature Control‏‎ (109,618 views)
  15. Error analysis‏‎ (109,401 views)
  16. Assignment 4 part 2: Measure resolution‏‎ (106,753 views)
  17. Assignment 1, Part 3: Building your transillumination microscope‏‎ (105,701 views)
  18. Optical Microscopy Part 1: Brightfield Microscopy‏‎ (103,988 views)
  19. Assignment 2: fluorescence microscopy‏‎ (96,546 views)
  20. Optical detectors, noise, and the limit of detection‏‎ (89,415 views)
  21. Impedance Analysis‏‎ (88,346 views)
  22. Assignment 1, Part 2: Optics bootcamp‏‎ (88,230 views)
  23. 20.109 Main Page‏‎ (85,858 views)
  24. Assignment 3, Part 1: visualizing actin with fluorescence contrast‏‎ (83,936 views)
  25. Bode plots‏‎ (83,420 views)
  26. Limits of Detection: Data Sessions‏‎ (83,215 views)
  27. Lab Manual: Limits of Detection‏‎ (83,067 views)
  28. Estimating second order system parameters from noise power spectra using nonlinear regression‏‎ (82,897 views)
  29. DNA Melting Report Requirements‏‎ (82,170 views)
  30. Assignment 1 Overview: Transillumination microscopy‏‎ (81,537 views)
  31. Understanding log plots‏‎ (77,984 views)
  32. DNA Melting: Model function and parameter estimation by nonlinear regression‏‎ (77,269 views)
  33. Todo List‏‎ (76,688 views)
  34. Assignment 4 part 1: Make a fake image‏‎ (76,148 views)
  35. Electronics written problems‏‎ (74,697 views)
  36. Assignment 1, Part 4: Measuring magnification and bead size‏‎ (73,360 views)
  37. DNA Melting: Simulating DNA Melting - Basics‏‎ (71,425 views)
  38. Optical Microscopy Part 3: Resolution and Stability‏‎ (71,180 views)
  39. Spring 2020 Assignment 9‏‎ (71,016 views)
  40. 20.109(F18): Fall 2018 schedule‏‎ (70,377 views)
  41. Exam 2 study guide‏‎ (67,651 views)
  42. Assignment 10, Part 1: Measuring the osmotic shock response of yeast‏‎ (65,003 views)
  43. Physical optics and resolution‏‎ (64,993 views)
  44. Assignment 8, Part 3: add flow control and test your device‏‎ (63,181 views)
  45. Lab Manual: Measuring DNA Melting Curves‏‎ (62,545 views)
  46. Optical Microscopy Part 2: Fluorescence Microscopy‏‎ (62,324 views)
  47. 20.109(S19): Spring 2019 schedule‏‎ (60,568 views)
  48. Assignment 2 Part 2: Fluorescence microscopy‏‎ (59,559 views)
  49. Protocols for cell culture‏‎ (58,663 views)
  50. Optical Microscopy Part 4: Particle Tracking‏‎ (58,230 views)
  51. 20.109(S17): Spring 2017 schedule‏‎ (57,472 views)
  52. Assignment 2 Part 3: Build an epi-illuminator for your microscope‏‎ (56,909 views)
  53. 20.109(F16): Fall 2016 schedule‏‎ (54,170 views)
  54. Microscopy report outline‏‎ (53,911 views)
  55. Electronics boot camp lab part 1‏‎ (53,539 views)
  56. 20.109(S20): Spring 2020 schedule‏‎ (53,186 views)
  57. 20.109(S18): Spring 2018 schedule‏‎ (52,407 views)
  58. Cell Reservations‏‎ (52,191 views)
  59. Assignment 1, Part 1: Pre-lab questions‏‎ (50,977 views)
  60. 20.109(F19):Class data‏‎ (50,055 views)
  61. Input and output impedance‏‎ (49,819 views)
  62. 20.109(S19):Class data‏‎ (49,813 views)
  63. 20.109(F20): Fall 2020 schedule‏‎ (49,215 views)
  64. 20.109(S18):Class data‏‎ (48,865 views)
  65. Manta G032 camera measurements‏‎ (48,277 views)
  66. Assignment 9, Part 1: Analyze two-color yeast images‏‎ (48,079 views)
  67. MATLAB: Estimating resolution from a PSF slide image‏‎ (47,844 views)
  68. DNA Melting Thermodynamics‏‎ (46,968 views)
  69. 20.109(S21):Spring 2021 schedule‏‎ (46,243 views)
  70. Complex Number Review‏‎ (45,602 views)
  71. Assignment 4 part 3: Track microspheres over time‏‎ (45,476 views)
  72. 20.109(F18):Class data‏‎ (45,218 views)
  73. 20.109(F17): Fall 2017 Schedule‏‎ (42,805 views)
  74. Assignment 8, Part 2: fabricate a microfluidic device‏‎ (41,960 views)
  75. 20.109(S21):M1D1‏‎ (40,925 views)
  76. 20.109(F16):Communication‏‎ (39,611 views)
  77. 20.109(F19): Fall 2019 schedule‏‎ (39,076 views)
  78. 20.109(F15):Homework‏‎ (37,898 views)
  79. DNA Melting Report Requirements for Part 1‏‎ (37,575 views)
  80. 20.309:Lab Report Guidelines‏‎ (36,026 views)
  81. 20.109(S11):Journal club (Day8)‏‎ (35,867 views)
  82. 20.109(S16):Homework‏‎ (35,307 views)
  83. Assignment 8, Part 0: convolution practice‏‎ (35,001 views)
  84. Spring 2012:Leanna Morinishi Lab 1‏‎ (34,979 views)
  85. Flat-field correction‏‎ (34,684 views)
  86. Converting Gaussian fit to Rayleigh resolution‏‎ (34,606 views)
  87. DNA melting: Identifying the unknown sample‏‎ (34,522 views)
  88. 20.109(F18):Homework‏‎ (34,232 views)
  89. 20.109(F16):Test comet chip loading variables (Day2)‏‎ (34,018 views)
  90. Fall 2012: Journal Presentations‏‎ (33,765 views)
  91. 20.109(S16):In situ cloning (Day1)‏‎ (33,570 views)
  92. ThorLabs OTKB optical trapping kit‏‎ (33,561 views)
  93. Assignment 8, Part 2: lock-in amplifier and temperature control‏‎ (32,665 views)
  94. Laser cutter safe operating procedure‏‎ (32,290 views)
  95. 20.109(S18):Journal club presentations (Day6 and 7)‏‎ (32,266 views)
  96. 20.109‏‎ (32,018 views)
  97. Optical Microscopy: Part 4 Report Outline‏‎ (31,204 views)
  98. 20.109(S16): Spring 2016 schedule‏‎ (31,104 views)
  99. Assignment 5 Overview‏‎ (30,225 views)
  100. 20.109(S21):M1D2‏‎ (29,805 views)
  101. 20.109(S20):Journal club presentations (Day4 and 5)‏‎ (29,307 views)
  102. 20.109(S19):Journal club presentations (Day7 and 8)‏‎ (29,304 views)
  103. Real electronics‏‎ (29,102 views)
  104. 20.109(F16):Homework‏‎ (27,283 views)
  105. Assignment 7, Part 2: measure temperature and fluorescence‏‎ (27,251 views)
  106. 20.109(S09): Module 2 oral presentations‏‎ (27,083 views)
  107. 20.109:People‏‎ (26,986 views)
  108. Assignment 3 Overview‏‎ (26,776 views)
  109. Spring 11:QRT-PCR‏‎ (26,558 views)
  110. 20.109(S20):Homework‏‎ (26,426 views)
  111. 20.109(S21):M3D3‏‎ (26,350 views)
  112. Calculating MSD and Diffusion Coefficients‏‎ (26,085 views)
  113. 20.109(S08):Data analysis (Day7)‏‎ (25,878 views)
  114. 20.109(F16):Lab tour‏‎ (25,651 views)
  115. 20.109(F12)‏‎ (25,030 views)
  116. 20.109(S21):Homework‏‎ (24,922 views)
  117. 20.109(S17):Homework‏‎ (24,859 views)
  118. 20.109(S13):Journal club II (Day8)‏‎ (24,741 views)
  119. 20.109(F15): Fall 2015 schedule‏‎ (24,561 views)
  120. 20.109(S16):Begin Western blot protein analysis and choose system conditions (Day2)‏‎ (24,558 views)
  121. Problem Set 1‏‎ (24,030 views)
  122. 20.109(F21):Homework‏‎ (23,761 views)
  123. Electronics bootcamp II: feedback systems‏‎ (23,292 views)
  124. 20.109(S21):M1D7‏‎ (22,959 views)
  125. Aperture and field stops‏‎ (22,746 views)
  126. 20.109(S18):Homework‏‎ (22,739 views)
  127. 20.109(S21):M2D6‏‎ (22,578 views)
  128. Optical aberrations‏‎ (22,465 views)
  129. 20.109(S14):Journal club II (Day8)‏‎ (22,390 views)
  130. 20.109(F17):Journal club II (Day 6)‏‎ (22,333 views)
  131. Spring 2011:Optical Trapping Lab‏‎ (22,297 views)
  132. Spring 2011:3D PSF lab‏‎ (22,277 views)
  133. 20.109(S16):Journal Club II (Day8)‏‎ (22,234 views)
  134. 20.109(S07):Start-up genome engineering‏‎ (21,869 views)
  135. 20.109(F20):Lab tour‏‎ (21,559 views)
  136. 20.109(S22):Class data‏‎ (21,444 views)
  137. 20.109(F21):Fall 2021 schedule‏‎ (21,350 views)
  138. 20.109(S17):Journal Club II (Day8)‏‎ (21,004 views)
  139. Assignment 10 Overview‏‎ (20,982 views)
  140. 20.109(F20):M3D4‏‎ (20,937 views)
  141. Understanding the lock-in amplifier‏‎ (20,878 views)
  142. Optical microscopy lab wiki pages‏‎ (20,735 views)
  143. 20.109(F16):Journal club II (Day 6)‏‎ (20,664 views)
  144. 20.109(S22):Spring 2022 schedule‏‎ (20,584 views)
  145. Sandbox‏‎ (20,464 views)
  146. Spring 2020 Assignment 8‏‎ (20,295 views)
  147. DNA melting lab wiki pages‏‎ (20,273 views)
  148. Assignment 5, Part 1: MSD difference tracking and microscope stability‏‎ (20,107 views)
  149. 20.109(S21):M1D4‏‎ (19,980 views)
  150. 20.109(F20):M1D2‏‎ (19,886 views)
  151. Assignment 4: finding and measuring things‏‎ (19,785 views)
  152. Spring 2012: Ajoke Jumoke Williams‏‎ (19,740 views)
  153. 20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)‏‎ (19,645 views)
  154. Confocal Wiki‏‎ (19,541 views)
  155. 20.109(F07):People‏‎ (19,514 views)
  156. Spring 2012:LFM Documentation‏‎ (19,513 views)
  157. Protocols‏‎ (19,238 views)
  158. Optical trap‏‎ (19,054 views)
  159. 20.109(S21):M3D2‏‎ (18,995 views)
  160. Williams: Optical Trapping Lab 1‏‎ (18,965 views)
  161. 20.109(F18):Journal club presentation (Day4 and 6)‏‎ (18,817 views)
  162. 20.109(F19):Journal club presentation (Day4 and 6)‏‎ (18,511 views)
  163. 20.109(S10):People‏‎ (18,417 views)
  164. 20.109(S07): Measuring calcium in vitro‏‎ (18,409 views)
  165. 20.109(F15):Journal Club II (Day8)‏‎ (18,378 views)
  166. Photobleaching Model‏‎ (18,267 views)
  167. Assignment 5, Part 2: live cell particle tracking of endocytosed beads‏‎ (18,231 views)
  168. 20.109(F11)‏‎ (18,195 views)
  169. 20.109(F12) Pre-Proposal: Engineering Viral Magnetic Nanoparticles for Magnetic Hyperthermic Cancer Therapy‏‎ (18,099 views)
  170. 20.109(S21):Journal club presentation‏‎ (18,089 views)
  171. In silico cloning of pdCas9 construct‏‎ (18,008 views)
  172. 20.109(S07): Student presentations, module 2‏‎ (18,005 views)
  173. 20.109(F20):M2D1‏‎ (17,996 views)
  174. Assignment 3, Part 2: experimental design with fluorescence‏‎ (17,794 views)
  175. 20.109(S21):M3D1‏‎ (17,789 views)
  176. Assignment 8 Overview: flow channel & two-color microscope‏‎ (17,658 views)
  177. 20.109: Spring 2007 schedule‏‎ (17,487 views)
  178. Assignment 8 Overview‏‎ (17,459 views)
  179. 20.109(S10):Journal club (Day8)‏‎ (17,397 views)
  180. 20.109(S21):M1D6‏‎ (17,373 views)
  181. 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7)‏‎ (17,324 views)
  182. 20.109(S19):Purify protein for secondary assays(Day2)‏‎ (17,305 views)
  183. Assignment 9 Overview‏‎ (17,292 views)
  184. 20.345:Main page‏‎ (17,212 views)
  185. 20.109(S21):M1D3‏‎ (17,204 views)
  186. Assignment 7 Overview‏‎ (16,991 views)
  187. Two-color Yeast Protocols‏‎ (16,917 views)
  188. 20.109(F20):M1D5‏‎ (16,835 views)
  189. Assignment 6, Part 2: Electronics bootcamp‏‎ (16,561 views)
  190. 20.109(F12): Fall 2012 schedule‏‎ (16,535 views)
  191. 20.109(S13): Spring 2013 schedule‏‎ (16,396 views)
  192. 20.109(F19):Homework‏‎ (16,272 views)
  193. Optical Microscopy Part 3: Resolution, Stability, and Particle Tracking‏‎ (16,180 views)
  194. Matlab:Image Processing for Fluorescent Microscopy‏‎ (16,155 views)
  195. 20.345:Final project proposal‏‎ (16,148 views)
  196. 20.109(S21):M3D4‏‎ (16,067 views)
  197. Assignment 8, Part 2: build a two-color microscope‏‎ (15,962 views)
  198. 20.109(F19):Treat cells for gamma-H2AX (Day2)‏‎ (15,957 views)
  199. 20.309:Safety‏‎ (15,887 views)
  200. Assignment 9, Part 1: model function‏‎ (15,871 views)
  201. 20.109(S07): Start-up expression engineering‏‎ (15,779 views)
  202. Temporary Optics Bootcamp‏‎ (15,731 views)
  203. 20.109(S14): Spring 2014 schedule‏‎ (15,706 views)
  204. 20.109(S23):Spring 2023 schedule‏‎ (15,691 views)
  205. Matlab: Scalebars‏‎ (15,652 views)
  206. 20.109(S17):Complete cell survival assay and examine transcript levels in response to DNA damage (Day 4)‏‎ (15,615 views)
  207. 20.109(S18):Confirm ligand binding using differential scanning fluorimetry assay (Day6)‏‎ (15,542 views)
  208. Spring 11:Chemotaxis Assay‏‎ (15,523 views)
  209. 20.109(S17):Purify RNA for quantitative PCR assay and treat cells for survival assay (Day3)‏‎ (15,335 views)
  210. 20.109(F16):Module 1‏‎ (15,327 views)
  211. 20.109(F15)‏‎ (15,320 views)
  212. Assignment 5, Part 1: viscosity and diffusivity in glycerol-water mixtures‏‎ (15,185 views)
  213. 20.109(F20):M2D3‏‎ (15,150 views)
  214. 20.109(S09):People‏‎ (15,062 views)
  215. 20.109(S20):Identify clones to characterize (Day3)‏‎ (14,999 views)
  216. 20.109(S18):Analyze small microarray data and induce protein expression (Day2)‏‎ (14,990 views)
  217. 20.109(S17):Confirm cell lines and practice tissue culture techniques (Day1)‏‎ (14,804 views)
  218. 20.109(F08): Mod 2 Day 1 Protein engineering with PCR‏‎ (14,795 views)
  219. 20.109(F16):Prepare comet chips (Day1)‏‎ (14,772 views)
  220. 20.109(S19):Evaluate protein purity and concentration (Day4)‏‎ (14,735 views)
  221. 20.109(S19):Homework‏‎ (14,626 views)
  222. 20.109(S07): Agarose gel electrophoresis‏‎ (14,522 views)
  223. Assignment 7, Part 1: op amp golden rules questions‏‎ (14,441 views)
  224. 20.109(F15):Prepare expression system (Day2)‏‎ (14,368 views)
  225. 20.109(F16): Assignments‏‎ (14,255 views)
  226. 20.109(S21):Module 1‏‎ (14,118 views)
  227. 20.109(F20):M1D6‏‎ (13,948 views)
  228. 20.109(S11):People‏‎ (13,908 views)
  229. 20.109(S16):Design mutation primers (Day2)‏‎ (13,843 views)
  230. 20.109(S20):Purify TDP43 protein (Day2)‏‎ (13,832 views)
  231. Procedure: Diffusion in biological gels‏‎ (13,803 views)
  232. Assignment 6 Overview‏‎ (13,741 views)
  233. Assignment 11: Tell us about your lab visit‏‎ (13,726 views)
  234. 20.109(S17):Complete data analysis (Day6)‏‎ (13,722 views)
  235. 20.109(F16):Confirm gRNA sequence (Day5)‏‎ (13,718 views)
  236. 20.109(F22):Fall 2022 schedule‏‎ (13,686 views)
  237. Spring 2012:Leanna Morinishi‏‎ (13,668 views)
  238. 20.109(F15):Induce protein and evaluate DNA (Day3)‏‎ (13,667 views)
  239. Using tfest to find a system model‏‎ (13,569 views)
  240. 20.109(F09): Fall 2009 schedule‏‎ (13,564 views)
  241. 20.109(F20):Homework‏‎ (13,555 views)
  242. 20.109(S20):Analyze titration curves (Day5)‏‎ (13,549 views)
  243. 20.109(F09)‏‎ (13,525 views)
  244. 20.109(F18):Analyze genomic instability experiment data and treat cells for sub-nuclear foci assay (Day5)‏‎ (13,469 views)
  245. Spring 2020 Assignment 6‏‎ (13,421 views)
  246. Theory‏‎ (13,377 views)
  247. BE Classroom Computer Documentation‏‎ (13,373 views)
  248. Assignment 9 Overview: Analyzing yeast images‏‎ (13,360 views)
  249. 20.109(F16):Complete in silico cloning (Day1)‏‎ (13,346 views)
  250. Resource list: DNA melting and PCR‏‎ (13,332 views)

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