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  1. Lab Manual: Atomic Force Microscopy (AFM)‏‎ (422,574 views)
  2. 20.309 Main Page‏‎ (326,979 views)
  3. DNA Melting Part 1: Measuring Temperature and Fluorescence‏‎ (293,442 views)
  4. Main Page‏‎ (291,614 views)
  5. Electronics primer‏‎ (247,985 views)
  6. Electronics Mini-Lab‏‎ (243,319 views)
  7. Geometrical optics and ray tracing‏‎ (206,065 views)
  8. Lab Manual: Optical Microscopy‏‎ (148,575 views)
  9. Assignment 2 Part 1: Noise in images‏‎ (145,419 views)
  10. 20.345:Course Information‏‎ (144,843 views)
  11. Optics Bootcamp‏‎ (124,608 views)
  12. 20.309:Course Information‏‎ (120,535 views)
  13. DNA Melting: Simulating DNA Melting - Intermediate Topics‏‎ (114,917 views)
  14. DNA Melting Part 2: Lock-in Amplifier and Temperature Control‏‎ (111,121 views)
  15. Error analysis‏‎ (110,057 views)
  16. Assignment 4 part 2: Measure resolution‏‎ (108,227 views)
  17. Assignment 1, Part 3: Building your transillumination microscope‏‎ (106,784 views)
  18. Optical Microscopy Part 1: Brightfield Microscopy‏‎ (104,456 views)
  19. Assignment 2: fluorescence microscopy‏‎ (97,405 views)
  20. Optical detectors, noise, and the limit of detection‏‎ (90,210 views)
  21. Assignment 1, Part 2: Optics bootcamp‏‎ (89,693 views)
  22. Impedance Analysis‏‎ (89,069 views)
  23. 20.109 Main Page‏‎ (86,516 views)
  24. Assignment 3, Part 1: visualizing actin with fluorescence contrast‏‎ (85,187 views)
  25. Bode plots‏‎ (84,714 views)
  26. Limits of Detection: Data Sessions‏‎ (83,875 views)
  27. Estimating second order system parameters from noise power spectra using nonlinear regression‏‎ (83,372 views)
  28. Lab Manual: Limits of Detection‏‎ (83,367 views)
  29. DNA Melting Report Requirements‏‎ (82,681 views)
  30. Assignment 1 Overview: Transillumination microscopy‏‎ (82,554 views)
  31. Understanding log plots‏‎ (78,570 views)
  32. DNA Melting: Model function and parameter estimation by nonlinear regression‏‎ (78,290 views)
  33. Assignment 4 part 1: Make a fake image‏‎ (77,324 views)
  34. Todo List‏‎ (76,901 views)
  35. Electronics written problems‏‎ (75,408 views)
  36. Assignment 1, Part 4: Measuring magnification and bead size‏‎ (74,560 views)
  37. Spring 2020 Assignment 9‏‎ (72,078 views)
  38. DNA Melting: Simulating DNA Melting - Basics‏‎ (71,796 views)
  39. Optical Microscopy Part 3: Resolution and Stability‏‎ (71,505 views)
  40. 20.109(F18): Fall 2018 schedule‏‎ (70,772 views)
  41. Exam 2 study guide‏‎ (67,992 views)
  42. Assignment 10, Part 1: Measuring the osmotic shock response of yeast‏‎ (66,197 views)
  43. Physical optics and resolution‏‎ (65,427 views)
  44. Assignment 8, Part 3: add flow control and test your device‏‎ (64,557 views)
  45. Lab Manual: Measuring DNA Melting Curves‏‎ (62,943 views)
  46. Optical Microscopy Part 2: Fluorescence Microscopy‏‎ (62,661 views)
  47. 20.109(S19): Spring 2019 schedule‏‎ (60,593 views)
  48. Assignment 2 Part 2: Fluorescence microscopy‏‎ (60,276 views)
  49. Protocols for cell culture‏‎ (59,117 views)
  50. Optical Microscopy Part 4: Particle Tracking‏‎ (58,431 views)
  51. Assignment 2 Part 3: Build an epi-illuminator for your microscope‏‎ (57,660 views)
  52. 20.109(S17): Spring 2017 schedule‏‎ (57,612 views)
  53. Electronics boot camp lab part 1‏‎ (54,473 views)
  54. 20.109(F16): Fall 2016 schedule‏‎ (54,473 views)
  55. Microscopy report outline‏‎ (54,046 views)
  56. 20.109(S20): Spring 2020 schedule‏‎ (53,772 views)
  57. 20.109(S18): Spring 2018 schedule‏‎ (52,801 views)
  58. Cell Reservations‏‎ (52,602 views)
  59. Assignment 1, Part 1: Pre-lab questions‏‎ (51,886 views)
  60. 20.109(F20): Fall 2020 schedule‏‎ (50,748 views)
  61. 20.109(F19):Class data‏‎ (50,344 views)
  62. Input and output impedance‏‎ (50,249 views)
  63. 20.109(S19):Class data‏‎ (50,155 views)
  64. 20.109(S18):Class data‏‎ (49,371 views)
  65. Assignment 9, Part 1: Analyze two-color yeast images‏‎ (49,320 views)
  66. Manta G032 camera measurements‏‎ (48,768 views)
  67. MATLAB: Estimating resolution from a PSF slide image‏‎ (48,176 views)
  68. 20.109(S21):Spring 2021 schedule‏‎ (48,032 views)
  69. DNA Melting Thermodynamics‏‎ (47,473 views)
  70. Complex Number Review‏‎ (46,408 views)
  71. Assignment 4 part 3: Track microspheres over time‏‎ (45,986 views)
  72. 20.109(F18):Class data‏‎ (45,742 views)
  73. 20.109(F17): Fall 2017 Schedule‏‎ (43,148 views)
  74. Assignment 8, Part 2: fabricate a microfluidic device‏‎ (42,541 views)
  75. 20.109(S21):M1D1‏‎ (41,444 views)
  76. 20.109(F16):Communication‏‎ (39,803 views)
  77. 20.109(F19): Fall 2019 schedule‏‎ (39,455 views)
  78. DNA Melting Report Requirements for Part 1‏‎ (38,166 views)
  79. 20.109(F15):Homework‏‎ (38,158 views)
  80. 20.309:Lab Report Guidelines‏‎ (36,197 views)
  81. 20.109(S11):Journal club (Day8)‏‎ (36,153 views)
  82. DNA melting: Identifying the unknown sample‏‎ (35,642 views)
  83. 20.109(S16):Homework‏‎ (35,451 views)
  84. Assignment 8, Part 0: convolution practice‏‎ (35,315 views)
  85. Spring 2012:Leanna Morinishi Lab 1‏‎ (35,194 views)
  86. Converting Gaussian fit to Rayleigh resolution‏‎ (34,918 views)
  87. Flat-field correction‏‎ (34,888 views)
  88. 20.109(F18):Homework‏‎ (34,524 views)
  89. 20.109(F16):Test comet chip loading variables (Day2)‏‎ (34,227 views)
  90. Fall 2012: Journal Presentations‏‎ (33,942 views)
  91. ThorLabs OTKB optical trapping kit‏‎ (33,868 views)
  92. 20.109(S16):In situ cloning (Day1)‏‎ (33,788 views)
  93. Assignment 8, Part 2: lock-in amplifier and temperature control‏‎ (33,091 views)
  94. Laser cutter safe operating procedure‏‎ (32,538 views)
  95. 20.109(S18):Journal club presentations (Day6 and 7)‏‎ (32,448 views)
  96. 20.109‏‎ (32,061 views)
  97. Optical Microscopy: Part 4 Report Outline‏‎ (31,382 views)
  98. 20.109(S16): Spring 2016 schedule‏‎ (31,321 views)
  99. 20.109(S21):M1D2‏‎ (30,750 views)
  100. Assignment 5 Overview‏‎ (30,508 views)
  101. 20.109(S20):Journal club presentations (Day4 and 5)‏‎ (29,606 views)
  102. 20.109(S19):Journal club presentations (Day7 and 8)‏‎ (29,376 views)
  103. Real electronics‏‎ (29,314 views)
  104. Assignment 7, Part 2: measure temperature and fluorescence‏‎ (27,769 views)
  105. 20.109(F16):Homework‏‎ (27,324 views)
  106. 20.109(S09): Module 2 oral presentations‏‎ (27,215 views)
  107. Assignment 3 Overview‏‎ (27,128 views)
  108. 20.109(S21):M3D3‏‎ (27,108 views)
  109. 20.109:People‏‎ (27,014 views)
  110. 20.109(S20):Homework‏‎ (26,669 views)
  111. Spring 11:QRT-PCR‏‎ (26,624 views)
  112. Calculating MSD and Diffusion Coefficients‏‎ (26,359 views)
  113. 20.109(S21):Homework‏‎ (26,103 views)
  114. 20.109(S08):Data analysis (Day7)‏‎ (26,024 views)
  115. 20.109(F16):Lab tour‏‎ (25,912 views)
  116. 20.109(F12)‏‎ (25,073 views)
  117. 20.109(S13):Journal club II (Day8)‏‎ (24,953 views)
  118. 20.109(S17):Homework‏‎ (24,877 views)
  119. 20.109(S16):Begin Western blot protein analysis and choose system conditions (Day2)‏‎ (24,876 views)
  120. 20.109(F15): Fall 2015 schedule‏‎ (24,729 views)
  121. 20.109(F21):Homework‏‎ (24,575 views)
  122. Problem Set 1‏‎ (24,062 views)
  123. 20.109(S21):M1D7‏‎ (23,949 views)
  124. Electronics bootcamp II: feedback systems‏‎ (23,640 views)
  125. 20.109(S21):M2D6‏‎ (23,153 views)
  126. 20.109(S18):Homework‏‎ (22,936 views)
  127. Aperture and field stops‏‎ (22,855 views)
  128. Optical aberrations‏‎ (22,635 views)
  129. 20.109(F21):Fall 2021 schedule‏‎ (22,615 views)
  130. 20.109(S14):Journal club II (Day8)‏‎ (22,605 views)
  131. 20.109(F17):Journal club II (Day 6)‏‎ (22,553 views)
  132. 20.109(S16):Journal Club II (Day8)‏‎ (22,409 views)
  133. Spring 2011:Optical Trapping Lab‏‎ (22,353 views)
  134. Spring 2011:3D PSF lab‏‎ (22,350 views)
  135. 20.109(S07):Start-up genome engineering‏‎ (22,025 views)
  136. 20.109(F20):Lab tour‏‎ (22,023 views)
  137. 20.109(S22):Class data‏‎ (21,948 views)
  138. 20.109(F20):M3D4‏‎ (21,632 views)
  139. 20.109(S22):Spring 2022 schedule‏‎ (21,514 views)
  140. Assignment 10 Overview‏‎ (21,235 views)
  141. 20.109(S21):M1D4‏‎ (21,087 views)
  142. 20.109(S17):Journal Club II (Day8)‏‎ (21,075 views)
  143. Understanding the lock-in amplifier‏‎ (21,027 views)
  144. Optical microscopy lab wiki pages‏‎ (20,865 views)
  145. 20.109(F16):Journal club II (Day 6)‏‎ (20,709 views)
  146. DNA melting lab wiki pages‏‎ (20,697 views)
  147. Sandbox‏‎ (20,544 views)
  148. Spring 2020 Assignment 8‏‎ (20,492 views)
  149. Assignment 5, Part 1: MSD difference tracking and microscope stability‏‎ (20,324 views)
  150. 20.109(F20):M1D2‏‎ (20,170 views)
  151. Assignment 4: finding and measuring things‏‎ (20,000 views)
  152. 20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)‏‎ (19,855 views)
  153. Spring 2012: Ajoke Jumoke Williams‏‎ (19,804 views)
  154. Spring 2012:LFM Documentation‏‎ (19,627 views)
  155. Confocal Wiki‏‎ (19,622 views)
  156. 20.109(F07):People‏‎ (19,533 views)
  157. Protocols‏‎ (19,276 views)
  158. 20.109(S21):M3D2‏‎ (19,191 views)
  159. Williams: Optical Trapping Lab 1‏‎ (19,183 views)
  160. Optical trap‏‎ (19,076 views)
  161. 20.109(F18):Journal club presentation (Day4 and 6)‏‎ (19,003 views)
  162. 20.109(F20):M2D1‏‎ (18,890 views)
  163. 20.109(F19):Journal club presentation (Day4 and 6)‏‎ (18,612 views)
  164. 20.109(S10):People‏‎ (18,458 views)
  165. Assignment 5, Part 2: live cell particle tracking of endocytosed beads‏‎ (18,437 views)
  166. 20.109(S07): Measuring calcium in vitro‏‎ (18,432 views)
  167. 20.109(F15):Journal Club II (Day8)‏‎ (18,412 views)
  168. Photobleaching Model‏‎ (18,373 views)
  169. 20.109(F11)‏‎ (18,216 views)
  170. 20.109(S21):Journal club presentation‏‎ (18,186 views)
  171. 20.109(F12) Pre-Proposal: Engineering Viral Magnetic Nanoparticles for Magnetic Hyperthermic Cancer Therapy‏‎ (18,126 views)
  172. Assignment 8 Overview: flow channel & two-color microscope‏‎ (18,117 views)
  173. 20.109(S07): Student presentations, module 2‏‎ (18,029 views)
  174. In silico cloning of pdCas9 construct‏‎ (18,027 views)
  175. 20.109(S21):M3D1‏‎ (18,011 views)
  176. Assignment 3, Part 2: experimental design with fluorescence‏‎ (18,000 views)
  177. 20.109(S21):M1D6‏‎ (17,984 views)
  178. 20.109(S21):M1D3‏‎ (17,961 views)
  179. Assignment 8 Overview‏‎ (17,709 views)
  180. Assignment 9 Overview‏‎ (17,699 views)
  181. 20.109: Spring 2007 schedule‏‎ (17,501 views)
  182. 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7)‏‎ (17,485 views)
  183. 20.109(S10):Journal club (Day8)‏‎ (17,439 views)
  184. Two-color Yeast Protocols‏‎ (17,398 views)
  185. 20.109(S19):Purify protein for secondary assays(Day2)‏‎ (17,323 views)
  186. 20.345:Main page‏‎ (17,322 views)
  187. Assignment 7 Overview‏‎ (17,250 views)
  188. 20.109(F20):M1D5‏‎ (17,064 views)
  189. Assignment 6, Part 2: Electronics bootcamp‏‎ (16,802 views)
  190. 20.109(S14): Spring 2014 schedule‏‎ (16,695 views)
  191. 20.109(F12): Fall 2012 schedule‏‎ (16,567 views)
  192. 20.345:Final project proposal‏‎ (16,535 views)
  193. 20.109(S21):M3D4‏‎ (16,447 views)
  194. 20.109(S13): Spring 2013 schedule‏‎ (16,404 views)
  195. Assignment 9, Part 1: model function‏‎ (16,351 views)
  196. 20.109(F19):Homework‏‎ (16,335 views)
  197. Optical Microscopy Part 3: Resolution, Stability, and Particle Tracking‏‎ (16,274 views)
  198. Matlab:Image Processing for Fluorescent Microscopy‏‎ (16,197 views)
  199. Assignment 8, Part 2: build a two-color microscope‏‎ (16,124 views)
  200. 20.109(F19):Treat cells for gamma-H2AX (Day2)‏‎ (16,034 views)
  201. 20.309:Safety‏‎ (15,985 views)
  202. Temporary Optics Bootcamp‏‎ (15,877 views)
  203. 20.109(S07): Start-up expression engineering‏‎ (15,876 views)
  204. 20.109(S18):Confirm ligand binding using differential scanning fluorimetry assay (Day6)‏‎ (15,759 views)
  205. 20.109(S23):Spring 2023 schedule‏‎ (15,752 views)
  206. Matlab: Scalebars‏‎ (15,735 views)
  207. 20.109(S17):Complete cell survival assay and examine transcript levels in response to DNA damage (Day 4)‏‎ (15,636 views)
  208. Spring 11:Chemotaxis Assay‏‎ (15,539 views)
  209. Assignment 5, Part 1: viscosity and diffusivity in glycerol-water mixtures‏‎ (15,399 views)
  210. 20.109(F15)‏‎ (15,390 views)
  211. 20.109(S17):Purify RNA for quantitative PCR assay and treat cells for survival assay (Day3)‏‎ (15,378 views)
  212. 20.109(F16):Module 1‏‎ (15,357 views)
  213. 20.109(F20):M2D3‏‎ (15,290 views)
  214. 20.109(S18):Analyze small microarray data and induce protein expression (Day2)‏‎ (15,225 views)
  215. 20.109(S09):People‏‎ (15,086 views)
  216. 20.109(S20):Identify clones to characterize (Day3)‏‎ (15,029 views)
  217. 20.109(S21):Module 1‏‎ (14,975 views)
  218. 20.109(F08): Mod 2 Day 1 Protein engineering with PCR‏‎ (14,967 views)
  219. 20.109(S17):Confirm cell lines and practice tissue culture techniques (Day1)‏‎ (14,826 views)
  220. 20.109(F16):Prepare comet chips (Day1)‏‎ (14,793 views)
  221. 20.109(S19):Evaluate protein purity and concentration (Day4)‏‎ (14,752 views)
  222. 20.109(S19):Homework‏‎ (14,647 views)
  223. Assignment 7, Part 1: op amp golden rules questions‏‎ (14,598 views)
  224. 20.109(F22):Fall 2022 schedule‏‎ (14,585 views)
  225. 20.109(S07): Agarose gel electrophoresis‏‎ (14,537 views)
  226. 20.109(F15):Prepare expression system (Day2)‏‎ (14,477 views)
  227. 20.109(F16): Assignments‏‎ (14,286 views)
  228. 20.109(F20):M1D6‏‎ (14,164 views)
  229. Assignment 11: Tell us about your lab visit‏‎ (13,997 views)
  230. 20.109(S16):Design mutation primers (Day2)‏‎ (13,969 views)
  231. 20.109(S11):People‏‎ (13,942 views)
  232. 20.109(S20):Purify TDP43 protein (Day2)‏‎ (13,864 views)
  233. Assignment 6 Overview‏‎ (13,856 views)
  234. Procedure: Diffusion in biological gels‏‎ (13,823 views)
  235. 20.109(F20):Homework‏‎ (13,782 views)
  236. 20.109(F15):Induce protein and evaluate DNA (Day3)‏‎ (13,766 views)
  237. Spring 2012:Leanna Morinishi‏‎ (13,749 views)
  238. 20.109(F16):Confirm gRNA sequence (Day5)‏‎ (13,740 views)
  239. 20.109(S17):Complete data analysis (Day6)‏‎ (13,740 views)
  240. Using tfest to find a system model‏‎ (13,661 views)
  241. Assignment 9 Overview: Analyzing yeast images‏‎ (13,655 views)
  242. 20.109(S20):Analyze titration curves (Day5)‏‎ (13,592 views)
  243. 20.109(F09): Fall 2009 schedule‏‎ (13,590 views)
  244. 20.109(F18):Analyze genomic instability experiment data and treat cells for sub-nuclear foci assay (Day5)‏‎ (13,579 views)
  245. 20.109(S21):M2D7‏‎ (13,566 views)
  246. 20.109(F09)‏‎ (13,551 views)
  247. Spring 2020 Assignment 6‏‎ (13,548 views)
  248. Theory‏‎ (13,433 views)
  249. BE Classroom Computer Documentation‏‎ (13,429 views)
  250. 20.109(F18): Assignments‏‎ (13,426 views)

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