DNA Melting Report Requirements for Part 1
From Course Wiki
- One member of your group should submit a single PDF file to Stellar in advance of the deadline. The filename should consist of the last names of all group members, CamelCased, in alphabetical order, with a .pdf extension. Example:
- Include answers to questions embedded in the lab manual (e.g. the questions in the RTD section)
- The file must be less than 20 MB.
- Include code at the end of the document in an appendix, in the same pdf file, not as as separate upload.
- Not counting the appendix, your report should be no longer than 10 pages.
Part 1 report outline
- Document your circuit design, optical design, and any ways that your instrument differs from the system described in the lab manual.
- Include values for all of the resistors, capacitors in your circuit.
- If you have not modified the circuits from their form in the report, you do not need to include the schematic in your report.
- Draw a block digram of your optical system, including focal lengths of lenses and key distances.
- Include a picture of your instrument.
- Report your signal to noise measurement.
- Plot at least one melting curve.
- The plot should have temperature in °C on the horizontal axis and fraction of double stranded DNA on the vertical axis.
- On the same set of axes, include a simulated curve generated by DINAMelt, OligoCalc, or another software simulator.
- Also on the same set of axes, plot the output of the DnaFraction function evaluated with best-fit parameters. You may use nlinfit to choose the best-fit parameters or you may choose them manually. (See: DNA Melting: Simulating DNA Melting - Basics)
- Include a legend.
- Report the estimated melting temperature and the best-fit values of ΔH°, ΔS°.
- Explain the statistical method you will use to identify your group's unknown sample in part 2 of this lab.
- State the acceptance/rejection criteria for any hypotheses tests you will use.
- This page may be a helpful reference: Identifying the unknown DNA sample.
Lab manual sections
- Lab Manual:Measuring DNA Melting Curves
- DNA Melting: Simulating DNA Melting - Basics
- DNA Melting Part 1: Measuring Temperature and Fluorescence
- DNA Melting Report Requirements for Part 1
- DNA Melting Part 2: Lock-in Amplifier and Temperature Control
- DNA Melting Report Requirements for Part 2