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  • DNA Melting Part 2: Lock-in Amplifier and Temperature Control
    ==Experimental procedure== ...e a different statistical procedure, if you like. Be sure to document the procedure you used.
    20 KB (3,227 words) - 20:05, 7 November 2017
  • DNA Melting Report Requirements
    # Procedure: #* Document the procedure used to gather your data.
    6 KB (864 words) - 18:50, 16 April 2017
  • DNA melting: Identifying the unknown sample
    ==Procedure== ...>multcompare</tt> function in MATLAB implements a correction to the t-test procedure to assure that the ''family-wise error rate'' (FWER) is less than the signi
    12 KB (1,931 words) - 18:49, 28 November 2017
  • 20.309:Lab Report Guidelines
    ===Explain the apparatus and procedure=== *Addresses shortcomings of the experimental procedure and problems that arose during experimentation
    10 KB (1,534 words) - 21:33, 13 March 2017
  • 20.309:Safety
    * Dispose of all materials properly. Ask an instructor is you are unsure of procedure.
    5 KB (821 words) - 16:50, 10 September 2015
  • Lab Manual: Atomic Force Microscopy (AFM)
    ... the sample and revisited for later samples. It is set in this calibration procedure by moving the laser spot so that the point of maximum sensitivity is arrive ... on having the correct value for "Scan sensitivity" (from your calibration procedure). The "Scan frequency" (lines per second) sets the speed of the tip across
    56 KB (9,320 words) - 20:42, 13 December 2017
  • ThorLabs OTKB optical trapping kit
    ===Alignment procedure=== ''Note: throughout this procedure, the Relay Lens (3A) will be called the Beam Expander Lens and Relay Lens I
    24 KB (4,010 words) - 15:32, 5 August 2010
  • Procedure: Diffusion in biological gels
    [[Category:Procedure]] {{Template:Safety Warning|message=This procedure uses sensitive biological samples. Wear gloves to avoid contamination.}}
    5 KB (878 words) - 23:26, 5 October 2010
  • Procedure: Particle tracking
    [[Category:Procedure]]
    5 KB (736 words) - 22:05, 4 October 2010
  • Microscopy report outline
    ... make. The measurement sections should include subsections that detail the procedure you used, data you gathered, analysis you did, and results you obtained. Yo
    2 KB (351 words) - 17:04, 11 January 2017
  • Optical Microscopy: Part 4 Report Outline
    <li>Procedure <li>Procedure
    3 KB (482 words) - 19:44, 14 March 2016
  • Procedure: Peptide-DNA Tethering Assay
    [[Category:Procedure]] {{Template:Safety Warning|message=This procedure uses sensitive biological samples. Wear gloves to avoid contamination.}}
    283 B (30 words) - 21:46, 23 February 2011
  • Spring 2011:Optical Trapping Lab
    The procedure depicted in the above graphs is repeated for each measurement, in each of t The procedure depicted above is repeated for several laser intensities and for each stage
    10 KB (1,595 words) - 16:29, 28 February 2012
  • DNA Melting: Simulating DNA Melting - Basics
    The procedure for creating the simulation is:
    13 KB (2,026 words) - 22:46, 15 April 2018
  • Photobleaching Model
    It looks like the general procedure is to point light at it and fry it. ...ll]: frequency-domain measurement using lock-in amplifier. A cool-sounding procedure, well suited to the existing DNA melter, and apparently also beneficial bec
    11 KB (1,755 words) - 23:31, 18 July 2011
  • Optical Microscopy: Part 3 Report Outline
    <li>Procedure <li>Procedure
    2 KB (355 words) - 19:04, 7 March 2016
  • Protocols for cell culture
    ...and gloves. Spray gloves with ethanol. (Repeat as necessary throughout the procedure.)
    18 KB (2,896 words) - 18:17, 25 February 2024
  • Protocols
    ...er with 70% ethanol. Periodically spray gloves with ethanol throughout the procedure.
    4 KB (644 words) - 18:24, 15 August 2013
  • Optical Microscopy Part 1: Brightfield Microscopy
    ...nd 1 μm silica microspheres as described in the magnification measurement procedure (40x objective only).
    23 KB (3,758 words) - 22:45, 12 January 2017
  • Optical Microscopy Part 3: Resolution and Stability
    ... falls on the first minimum of the other source’s image. This suggests a procedure for measuring resolution: image a point source; measure the peak-to-trough ...necessary adjustments to your microscope and repeat this particle-tracking procedure to attain sufficient stability:
    7 KB (1,168 words) - 18:12, 29 September 2016
  • Lab Manual: Limits of Detection
    ==Optical trap procedure== ==Optical trap procedure==
    13 KB (2,117 words) - 17:08, 4 January 2016
  • Nonlinear regression
    ...ring the the extension. Regardless of the two force conditions chosen, the procedure provides two, datapoints, (<i>x<sub>i</sub></i>, <i>y<sub>i</sub></i>); <i>
    5 KB (774 words) - 18:07, 13 January 2014
  • Locating objects in a fluorescent microscopic image
    ==Procedure==
    3 KB (466 words) - 21:54, 4 January 2013
  • Electronics Mini-Lab
    ====Procedure====
    35 KB (5,891 words) - 18:46, 7 April 2017
  • Aligning the optical trap
    For this part of the procedure, you will need:
    4 KB (645 words) - 22:09, 3 May 2013
  • MATLAB: Estimating resolution from a PSF slide image
    ..., and divide by the magnification. In this part of the lab, you will use a procedure inspired by this simple idea to estimate the resolution of your microscope.
    17 KB (1,769 words) - 14:21, 30 September 2017
  • Error analysis
    A ''measurement procedure'' is set of actions that strive to reveal the value of an unknown physical ...was obtained just by chance or because of a shortcoming of the measurement procedure. Conclusions drawn from measured quantities must be considered in the conte
    24 KB (3,757 words) - 14:57, 3 September 2018
  • Optical Microscopy: Part 1 Report Outline
    ## Procedure ## Procedure
    2 KB (267 words) - 04:11, 27 July 2015
  • Optics Bootcamp
    ...ww.thorlabs.com ThorLabs website] can be very helpful. For example, if the procedure calls for an SPW602 spanner wrench and you have no idea what such a thing m * Repeat the procedure at several image and object distances. Do an equal number with the target p
    28 KB (4,857 words) - 20:09, 31 July 2017
  • Estimating second order system parameters from noise power spectra using nonlinear regression
    ...r confidence interval like this: Imagine that you repeated the measurement procedure precisely the same way 100 times. Because of random noise alone, the values
    30 KB (4,815 words) - 21:47, 27 December 2015
  • 20.109(S08):Characterize protein expression (Day5)
    ...lyse the bacteria, and run whole bacterial extracts on a protein gel. This procedure is called SDS-PAGE, for sodium docecyl sulfate-polyacrylamide gel electroph ...rocess three samples (the three +IPTG extracts) according to the following procedure. You should either time your spins with another group, or balance your tube
    12 KB (1,946 words) - 16:04, 15 June 2015
  • 20.109(S08):Preparing cells for analysis (Day4)
    #Follow the Day 3 procedure (trypsin for monolayer culture, EDTA-citrate for alginate culture) to recov ...ated to 50 &deg;C while you prepare your samples. This is required for the procedure to work optimally.
    16 KB (2,751 words) - 16:04, 15 June 2015
  • 20.109(S08):DNA engineering/DNA ligation and bacterial transformation (Day 4)
    ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
    10 KB (1,704 words) - 14:24, 5 June 2015
  • 20.109(S08):DNA engineering/Examine candidate clones (Day 5)
    ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
    10 KB (1,634 words) - 14:24, 5 June 2015
  • 20.109(S08):DNA engineering/FACS analysis (Day 8)
    ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
    5 KB (750 words) - 14:24, 5 June 2015
  • 20.109(S08):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
    7 KB (1,095 words) - 14:25, 5 June 2015
  • 20.109(S07): Bio-material engineering/Rescreening gold binders
    ...you might want to modify the standard protocol. Otherwise, follow the same procedure as you did for the initial library screen.
    5 KB (887 words) - 15:32, 15 June 2015
  • 20.109(S07):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
    6 KB (1,050 words) - 15:32, 15 June 2015
  • 20.109(F07):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
    6 KB (1,073 words) - 15:54, 15 June 2015
  • 20.109(F07): DNA ligation and bacterial transformation
    ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10^
    16 KB (2,748 words) - 15:54, 15 June 2015
  • 20.109(F08):DNA engineering/Examine candidate clones (Day 5)
    ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
    10 KB (1,634 words) - 19:05, 28 July 2015
  • 20.109(F08):DNA engineering/DNA ligation and bacterial transformation (Day 4)
    ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
    10 KB (1,704 words) - 19:05, 28 July 2015
  • 20.109(F08):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
    6 KB (1,075 words) - 19:05, 28 July 2015
  • 20.109(F08):DNA engineering/FACS analysis (Day 8)
    ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
    5 KB (744 words) - 19:05, 28 July 2015
  • 20.109(F08): Mod 1 Day 8 FACS analysis
    ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
    4 KB (676 words) - 19:05, 28 July 2015
  • 20.109(F08): Mod 1 Day 5 Examine candidate clones
    ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
    11 KB (1,770 words) - 19:05, 28 July 2015
  • 20.109(F08): Mod 1 Day 4 DNA ligations and bacterial transformations
    ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
    10 KB (1,617 words) - 19:05, 28 July 2015
  • 20.109(S09):Site-directed mutagenesis (Day2)
    ...smid DNA you used in your mutagenesis reaction, assuming that the miniprep procedure results in typical concentrations of 1 &mu;g/&mu;L.
    13 KB (2,155 words) - 19:33, 28 July 2015
  • 20.109(S09):Bacterial amplification of DNA (Day3)
    ...smid, you might increase the amount of DNA used during the transformation procedure at the end of lab. In between electrophoresis and transformation, we will h
    13 KB (2,203 words) - 19:33, 28 July 2015
  • 20.109(S09):Prepare expression system (Day4)
    ...rm what is commonly called a mini-prep. This term distinguishes the procedure from a maxi- or large scale-prep which involves a larger volume
    21 KB (3,468 words) - 19:33, 28 July 2015
  • 20.109(S09):Characterize protein expression (Day6)
    ...lyse the bacteria, and run whole bacterial extracts on a protein gel. This procedure is called SDS-PAGE, for sodium docecyl sulfate-polyacrylamide gel electroph ...rocess three samples (the three +IPTG extracts) according to the following procedure. You should either time your spins with another group, or balance your tube
    14 KB (2,203 words) - 19:33, 28 July 2015
  • 20.109(F09): Mod 1 Day 4 DNA ligations and bacterial transformations
    ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
    10 KB (1,761 words) - 19:38, 28 July 2015
  • 20.109(F09): Mod 1 Day 5 Examine candidate clones
    ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
    11 KB (1,830 words) - 19:38, 28 July 2015
  • 20.109(F09): Mod 1 Day 8 FACS analysis
    ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
    5 KB (804 words) - 19:38, 28 July 2015
  • 20.109(F09):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
    6 KB (1,084 words) - 19:38, 28 July 2015
  • 20.109(F10):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
    6 KB (1,084 words) - 21:00, 28 July 2015
  • 20.109(F10): Mod 1 Day 4 DNA ligations and bacterial transformations
    ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
    11 KB (1,791 words) - 21:00, 28 July 2015
  • 20.109(F10): Mod 1 Day 5 Examine candidate clones
    ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
    11 KB (1,830 words) - 21:00, 28 July 2015
  • 20.109(S11):RNA to DNA by RT-PCR (Day5)
    #Resuspend each sample in 44 &mu;L of RNase-free water by the following procedure: ...deg;C while you prepare your samples. This preparation is required for the procedure to work optimally.
    8 KB (1,243 words) - 21:05, 28 July 2015
  • 20.109(F11):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
    10 KB (1,639 words) - 21:08, 28 July 2015
  • 20.109(F11): Mod 1 Day 4 DNA ligations and bacterial transformations
    ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10< ... of the card if you wish. Your card will be all the information about this procedure that you can use for the [[20.109(F11):DNA engineering lab practical| lab p
    12 KB (1,904 words) - 21:08, 28 July 2015
  • 20.109(F11): Mod 1 Day 5 Examine candidate clones & tissue culture
    ...rm what is commonly called a mini-prep. This term distinguishes the procedure from a maxi- or large scale-prep which involves a larger volume
    13 KB (2,328 words) - 21:08, 28 July 2015
  • 20.109(F11): Mod 1 Day 7 FACS analysis
    ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
    5 KB (840 words) - 21:08, 28 July 2015
  • 20.109(S13):Bacterial amplification of DNA (Day3)
    ...smid, you might increase the amount of DNA used during the transformation procedure at the end of lab.
    13 KB (2,181 words) - 13:48, 29 July 2015
  • 20.109(S13):Purify protein (Day6)
    ...teria and save the whole cell extracts to later run on a protein gel. This procedure is called SDS-PAGE, for sodium docecyl sulfate-polyacrylamide gel electroph ...rocess three samples (the three +IPTG extracts) according to the following procedure. You should either time your spins with another group, or balance your tube
    13 KB (2,134 words) - 13:48, 29 July 2015
  • 20.109(S13): Protein engineering report
    ... in Module 1, assuming a niche audience who is somewhat familiar with each procedure. Use the published scientific literature as a guide, and do not omit ''too'
    5 KB (674 words) - 13:48, 29 July 2015
  • 20.109(S13):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
    7 KB (1,175 words) - 13:48, 29 July 2015
  • 20.109(S14):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your work space. Having a clear work s ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
    7 KB (1,207 words) - 13:59, 29 July 2015
  • 20.109(F13): TA notes for module 1
    * Pre-run entire cloning procedure (Day 1-4) and save materials in case something goes wrong in the student's
    16 KB (2,601 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 1 Day 2 Clean and cut DNA
    The procedure below reflects just one approach you may take to solving this assignment wi
    11 KB (1,780 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 1 Day 4 DNA ligations and bacterial transformations
    ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10< #Wash the pellets with 500 &mu;l 70% cold ethanol. This procedure is done by dribbling the 70% ethanol along the wall of the eppendorf tube t
    11 KB (1,836 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 1 Day 5 Examine candidate clones & tissue culture
    ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
    20 KB (3,455 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 1 Day 7 FACS analysis
    ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
    6 KB (1,033 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 1 Day 4 DNA ligations, bacterial transformations and CometChip
    ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10< #Wash the pellets with 500 &mu;l 70% cold ethanol. This procedure is done by dribbling the 70% ethanol along the wall of the eppendorf tube t
    12 KB (2,060 words) - 14:02, 29 July 2015
  • 20.109(F14): TA notes for module 1
    * Pre-run entire cloning procedure (Day 1-4) and save materials in case something goes wrong in the student's
    16 KB (2,511 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 3 Day 4 Solar cell assembly
    ...w An animation video of how DSSC's are assembled]. Remember, Dr. Belcher's procedure is a little different from what is described here, but it provides a good v
    11 KB (1,798 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 1 Day 4 DNA Ligation & Transformation
    ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10< #Wash the pellets with 500 &mu;l 70% cold ethanol. This procedure is done by dribbling the 70% ethanol along the wall of the eppendorf tube t
    12 KB (2,028 words) - 14:02, 29 July 2015
  • 20.109(F14):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
    7 KB (1,163 words) - 14:02, 29 July 2015
  • 20.109(F14):Module 3
    ...w An animation video of how DSSC's are assembled]. Remember, Dr. Belcher's procedure is a little different from what is described here, but it provides a good v
    4 KB (612 words) - 14:02, 29 July 2015
  • 20.109(S15):DNA cloning (Day4)
    ... and growing the bacteria in said antibiotic. (We’ll say more about that procedure below.) This method is especially suitable for cloning with the purpose of ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
    16 KB (2,696 words) - 17:15, 29 July 2015
  • 20.109(S15):DNA repair assays (Day5)
    ...just for counting and not for separating subpopulations of cells, then the procedure is called flow cytometry, while cell sorting or isolation is called FACS. H
    14 KB (2,396 words) - 17:15, 29 July 2015
  • 20.109(S15):DNA sequencing (Day5)
    The procedure for DNA isolation at this scale is commonly termed "mini-prep," which disti Normally in 20.109 we do an in-house mini-prep procedure according to the steps above followed by ethanol precipitation. However, be
    16 KB (2,688 words) - 17:15, 29 July 2015
  • 20.109(S15):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
    7 KB (1,162 words) - 17:15, 29 July 2015
  • 20.109(S15):Module 3
    ...w An animation video of how DSSC's are assembled]. Remember, Dr. Belcher's procedure is a little different from what is described here, but it provides a good v
    4 KB (601 words) - 17:15, 29 July 2015
  • 20.109(S15):Solar cell assembly (Day 4)
    ...w An animation video of how DSSC's are assembled]. Remember, Dr. Belcher's procedure is a little different from what is described here, but it provides a good v
    13 KB (2,017 words) - 17:15, 29 July 2015
  • 20.109(S12):RNA to DNA by RT-PCR (Day5)
    #Resuspend each sample in 44 &mu;L of RNase-free water by the following procedure: ...deg;C while you prepare your samples. This preparation is required for the procedure to work optimally.
    8 KB (1,365 words) - 17:30, 29 July 2015
  • 20.109(S12):Bacterial amplification of DNA (Day3)
    ...smid, you might increase the amount of DNA used during the transformation procedure at the end of lab.
    17 KB (2,889 words) - 17:30, 29 July 2015
  • 20.109(S12):Prepare expression system (Day4)
    ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
    19 KB (3,278 words) - 17:30, 29 July 2015
  • 20.109(S12):Characterize protein expression (Day6)
    ...teria and save the whole cell extracts to later run on a protein gel. This procedure is called SDS-PAGE, for sodium docecyl sulfate-polyacrylamide gel electroph ...rocess three samples (the three +IPTG extracts) according to the following procedure. You should either time your spins with another group, or balance your tube
    13 KB (2,041 words) - 17:30, 29 July 2015
  • 20.109(S12): Protein engineering report
    ... in Module 1, assuming a niche audience who is somewhat familiar with each procedure. Use the published scientific literature as a guide, and do not omit ''too'
    5 KB (776 words) - 17:30, 29 July 2015
  • 20.109(S12):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
    7 KB (1,163 words) - 17:30, 29 July 2015
  • 20.109(F13): Mod 1 Day 2 Clean and cut DNA
    The procedure below reflects just one approach you may take to solving this assignment wi
    11 KB (1,881 words) - 17:33, 29 July 2015
  • 20.109(F13): Mod 1 Day 7 FACS analysis
    ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
    6 KB (1,071 words) - 17:33, 29 July 2015
  • 20.109(F13): Mod 1 Day 4 DNA ligations and bacterial transformations
    ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10< #Wash the pellets with 500 &mu;l 70% cold ethanol. This procedure is done by dribbling the 70% ethanol along the wall of the eppendorf tube t
    11 KB (1,914 words) - 17:33, 29 July 2015
  • 20.109(F13): Mod 1 Day 5 Examine candidate clones & tissue culture
    ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
    17 KB (2,866 words) - 17:33, 29 July 2015
  • 20.109(F13):Module 3
    ...w An animation video of how DSSC's are assembled]. Remember, Dr. Belcher's procedure is a little different from what is described here, but it provides a good v
    4 KB (557 words) - 17:33, 29 July 2015
  • 20.109(F13):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
    7 KB (1,163 words) - 17:33, 29 July 2015
  • 20.109(F13): Mod 3 Day 4 Solar cell assembly
    ...w An animation video of how DSSC's are assembled]. Remember, Dr. Belcher's procedure is a little different from what is described here, but it provides a good v
    13 KB (2,096 words) - 17:33, 29 July 2015
  • 20.109(F13): System engineering report
    *You can assume a niche audience who is somewhat familiar with each procedure. Use the published scientific literature as a guide, and do not omit ''too'
    10 KB (1,586 words) - 17:33, 29 July 2015
  • 20.109(F13): Mod 2 Day 2 Mutation Analysis
    ...t up sequencing reactions and send to Genewiz. We will talk about how this procedure was done on M2D3.
    18 KB (2,944 words) - 17:33, 29 July 2015
  • 20.109(S10):Characterize protein expression (Day6)
    ...lyse the bacteria, and run whole bacterial extracts on a protein gel. This procedure is called SDS-PAGE, for sodium docecyl sulfate-polyacrylamide gel electroph ...rocess three samples (the three +IPTG extracts) according to the following procedure. You should either time your spins with another group, or balance your tube
    14 KB (2,165 words) - 19:48, 28 July 2015
  • 20.109(S10):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
    7 KB (1,148 words) - 19:49, 28 July 2015
  • 20.109(S10):RNA to DNA by RT-PCR (Day5)
    #Resuspend each sample in 44 &mu;L of RNase-free water by the following procedure: ...deg;C while you prepare your samples. This preparation is required for the procedure to work optimally.
    7 KB (1,218 words) - 19:49, 28 July 2015
  • 20.109(S10):Site-directed mutagenesis (Day2)
    ...smid DNA you used in your mutagenesis reaction, assuming that the miniprep procedure results in typical concentrations of 1 &mu;g/&mu;L.
    13 KB (2,131 words) - 19:49, 28 July 2015
  • 20.109(S10):Bacterial amplification of DNA (Day3)
    ...smid, you might increase the amount of DNA used during the transformation procedure at the end of lab.
    11 KB (1,861 words) - 19:49, 28 July 2015
  • 20.109(S10):Prepare expression system (Day4)
    ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
    22 KB (3,701 words) - 19:49, 28 July 2015
  • 20.109(S10):Preparing cells for analysis (Day4)
    ...ated to 50 &deg;C while you prepare your samples. This is required for the procedure to work optimally.
    15 KB (2,534 words) - 19:49, 28 July 2015
  • 20.109(S11):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
    7 KB (1,163 words) - 21:05, 28 July 2015
  • 20.109(F12): Mod 1 Day 5 Examine candidate clones & tissue culture
    ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
    13 KB (2,328 words) - 13:41, 29 July 2015
  • 20.109(F12): Mod 1 Day 7 FACS analysis
    ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
    5 KB (842 words) - 13:41, 29 July 2015
  • 20.109(F12):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
    10 KB (1,639 words) - 13:41, 29 July 2015
  • 20.109(F12): Mod 1 Day 4 DNA ligations and bacterial transformations
    ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
    12 KB (1,926 words) - 13:41, 29 July 2015
  • 20.109(S13):DNA cloning (Day4)
    ... and growing the bacteria in said antibiotic. (We’ll say more about that procedure below.) This method is especially suitable for cloning with the purpose of ...2, and Shannon's band was brighter, then Shannon and Agi would perform the procedure below with Shannon's PCR, but they wouldn't throw away Agi's PCR either.
    16 KB (2,679 words) - 13:47, 29 July 2015
  • 20.109(S13):DNA sequencing and primer analysis (Day5)
    The procedure for DNA isolation at this scale is commonly termed "mini-prep," which disti Normally in 20.109 we do an in-house mini-prep procedure according to the steps above followed by ethanol precipitation. However, be
    16 KB (2,629 words) - 13:48, 29 July 2015
  • 20.109(S13):Phylogenetic analysis (Day7)
    ...e an insert. However, note that there is a known problem with this cloning procedure wherein sometimes an incomplete vector (with no insert and also missing a c
    17 KB (2,753 words) - 13:48, 29 July 2015
  • 20.109(S14):DNA cloning (Day4)
    ... and growing the bacteria in said antibiotic. (We’ll say more about that procedure below.) This method is especially suitable for cloning with the purpose of ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
    15 KB (2,521 words) - 13:58, 29 July 2015
  • 20.109(S14):DNA sequencing (Day5)
    The procedure for DNA isolation at this scale is commonly termed "mini-prep," which disti Normally in 20.109 we do an in-house mini-prep procedure according to the steps above followed by ethanol precipitation. However, be
    17 KB (2,778 words) - 13:58, 29 July 2015
  • 20.109(S14):DNA repair assays(Day6)
    ...just for counting and not for separating subpopulations of cells, then the procedure is called flow cytometry, while cell sorting or isolation is called FACS. H #*Each T25 flask of cells will be trypsinized according to the M2D1 procedure.
    17 KB (2,912 words) - 13:59, 29 July 2015
  • 20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)
    ... to confirm the appropriate mutation was incorporated. The transformation procedure will be as follows:
    28 KB (4,525 words) - 14:45, 12 November 2015
  • 20.109(F15):Bacterial amplification of DNA (Day3)
    ...smid, you might increase the amount of DNA used during the transformation procedure at the end of lab.
    13 KB (2,207 words) - 11:40, 4 September 2015
  • 20.109(F15):Purify protein (Day4)
    ...teria and save the whole cell extracts to later run on a protein gel. This procedure is called SDS-PAGE, for sodium dodecyl sulfate-polyacrylamide gel electroph ... samples (+IPTG wild-type and +IPTG mutant IPC) according to the following procedure. <!--
    13 KB (2,001 words) - 18:01, 11 November 2015
  • 20.109(F15): TA notes for module 1
    * Pre-run entire cloning procedure (Day 1-4) and save materials in case something goes wrong in the student's
    16 KB (2,554 words) - 17:19, 12 January 2016
  • 20.109(F15):FACS analysis (Day7)
    ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
    6 KB (1,059 words) - 14:29, 2 October 2015
  • 20.109(F15):Clean and cut DNA (Day2)
    The procedure below reflects just one approach you may take to solving this assignment wi
    11 KB (1,805 words) - 19:52, 18 September 2015
  • 20.109(F15):DNA ligation & transformation (Day4)
    ... gently, specifically '''kept cold and not vortexed'''. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10< #Wash the pellets with 500 &mu;L 70% cold ethanol. This procedure is done by dribbling the 70% ethanol along the wall of the eppendorf tube t
    11 KB (1,745 words) - 20:32, 24 September 2015
  • 20.109(F15):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
    7 KB (1,217 words) - 11:40, 4 September 2015
  • 20.109(F15):Examine candidate clones & tissue culture (Day5)
    ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
    21 KB (3,522 words) - 18:18, 30 September 2015
  • 20.109(F15): Protein engineering report
    ...e your audience is scientifically literate and somewhat familiar with each procedure. Remember that the methods should be divided into sub-sections that do not
    8 KB (1,190 words) - 18:12, 9 November 2015
  • 20.109(F15):Homework
    ... a way that can be followed by someone less familiar with the experimental procedure? Does the author use concentrations to convey amount?
    28 KB (4,514 words) - 20:04, 2 December 2015
  • Manta G032 camera measurements
    ==Measurement procedure==
    5 KB (856 words) - 00:26, 22 October 2015
  • 20.109(F15):Battery assembly and testing (Day5)
    ...or negative current to charge or discharge the batteries, identical to the procedure used to test your battery. By attaching a battery to a Galvanostat and appl ...way. Thank you, George! Today you will see a demo of the battery assembly procedure. The steps are provided below for your notes.
    9 KB (1,418 words) - 18:31, 2 December 2015
  • 20.109(S16):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
    7 KB (1,217 words) - 16:21, 9 December 2015
  • 20.109(S16):Growth of phage materials (Day1)
    ... complete the next step in generating your nanowires. In this part of the procedure the phage-AuNP complexes will be biomineralized with FePO<sub>4</sub>. The
    16 KB (2,684 words) - 18:48, 15 April 2016
  • 20.109(S16):Battery assembly and testing (Day5)
    ...or negative current to charge or discharge the batteries, identical to the procedure used to test your battery. By attaching a battery to a galvanostat and appl ... away. Thank you, Jifa! Today you will see a demo of the battery assembly procedure. The steps are provided below for your notes.
    8 KB (1,384 words) - 17:28, 5 May 2016
  • 20.109(S16):Introduction to cell strains and plating (Day1)
    ...our cells, take the flask to your tissue culture hood to begin the seeding procedure.
    16 KB (2,780 words) - 17:34, 19 September 2016
  • 20.109(S16):Begin Western blot protein analysis and choose system conditions (Day2)
    ...the proteins on a polyacrylamide gel. You will also begin the Western blot procedure by transferring the separated proteins from your polyacrylamide gel onto a ... '''It is very important that you keep the cell lysate cold throughout the procedure.'''
    21 KB (3,393 words) - 15:00, 11 March 2016
  • 20.109(S16):Cell preparation for DNA repair assays (Day5)
    #Pre-label all of the eppendorf tubes that you will need for the transfection procedure.
    16 KB (2,542 words) - 18:34, 28 March 2016
  • 20.109(S16):DNA repair assays (Day6)
    ...hine is only able to count, and not sort subpopulations of cells, then the procedure is called flow cytometry, while cell sorting or isolation is called FACS. B
    14 KB (2,303 words) - 14:32, 14 April 2016
  • 20.109(S16):Site-directed mutagenesis (Day3)
    ...ng|thumb|center|550px|'''Schematic of NEB Q5 Site Directed Mutagenesis Kit procedure modified from NEB manual.''']] ... to confirm the appropriate mutation was incorporated. The transformation procedure will be as follows:
    13 KB (2,066 words) - 14:08, 17 February 2016
  • Temporary Optics Bootcamp
    ...ww.thorlabs.com ThorLabs website] can be very helpful. For example, if the procedure calls for an SPW602 spanner wrench and you have no idea what such a thing m
    15 KB (2,559 words) - 21:32, 2 February 2016
  • 20.109(S16):Prepare expression system (Day4)
    The procedure for DNA isolation using small volumes is commonly termed "mini-prep," which
    17 KB (2,817 words) - 22:14, 21 February 2016
  • 20.109(S16):Homework
    #*Include only key aspects of the mutagenesis procedure that were used to generate your IPC X#Z mutant. ... a way that can be followed by someone less familiar with the experimental procedure? Does the author use concentrations to convey amount?
    25 KB (3,974 words) - 17:41, 5 May 2016
  • 20.109(S16):Purify protein (Day6)
    ...teria and save the whole cell extracts to later run on a protein gel. This procedure is called SDS-PAGE, for sodium dodecyl sulfate-polyacrylamide gel electroph ... samples (+IPTG wild-type and +IPTG mutant IPC) according to the following procedure. <!--
    14 KB (2,249 words) - 20:15, 26 February 2016
  • 20.109(F16):Module 1
    A video of the procedure can be found [http://www.jove.com/video/50607/cometchip-high-throughput-96-
    3 KB (451 words) - 13:43, 23 September 2016
  • 20.109(F16):Clean and cut DNA (Day2)
    The procedure below reflects just one approach you may take to solving this assignment wi
    11 KB (1,805 words) - 15:11, 18 May 2016
  • 20.109(F16):DNA ligation & transformation (Day4)
    ... gently, specifically '''kept cold and not vortexed'''. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10< #Wash the pellets with 500 &mu;L 70% cold ethanol. This procedure is done by dribbling the 70% ethanol along the wall of the eppendorf tube t
    11 KB (1,745 words) - 15:25, 18 May 2016
  • 20.109(F16):Examine candidate clones & tissue culture (Day5)
    ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
    21 KB (3,522 words) - 15:28, 18 May 2016
  • 20.109(F16):FACS analysis (Day7)
    ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
    6 KB (1,054 words) - 15:37, 18 May 2016
  • 20.109(F16):Growth of phage materials (Day1)
    ... complete the next step in generating your nanowires. In this part of the procedure the phage-AuNP complexes will be biomineralized with FePO<sub>4</sub>. The
    16 KB (2,648 words) - 21:20, 16 November 2016
  • 20.109(F16):Battery assembly and testing (Day5)
    ...or negative current to charge or discharge the batteries, identical to the procedure used to test your battery. By attaching a battery to a galvanostat and appl ... away. Thank you, Jifa! Today you will see a demo of the battery assembly procedure. The steps are provided below for your notes.
    8 KB (1,380 words) - 19:32, 5 December 2016
  • 20.109(F16):Homework
    ... a way that can be followed by someone less familiar with the experimental procedure? Does the author use concentrations to convey amount?
    21 KB (3,289 words) - 21:26, 30 November 2016
  • Optical Microscopy Part 3: Resolution, Stability, and Particle Tracking
    ...; and divide by the magnification. In this part of the lab, you will use a procedure inspired by this simple idea to estimate the resolution of your microscope. ====Procedure====
    16 KB (2,559 words) - 22:47, 15 March 2017
  • Part 3 Combined Report Outline
    <li>Procedure <li>Procedure
    5 KB (806 words) - 16:00, 14 March 2017
  • 20.109(F16):Test comet chip loading variables (Day2)
    #'''Read through Steps #13-16 before continuing with the procedure.'''
    15 KB (2,594 words) - 17:33, 22 September 2016
  • 20.109(F16):Test comet chip assay variables (Day3)
    You will load comet chips prepared by the teaching faculty using the procedure from [http://engineerbiology.org/wiki/20.109%28F16%29:Test_comet_chip_loadi
    5 KB (878 words) - 19:20, 18 July 2016
  • 20.109(F16):Seed cells for immuno-flourescence assay (Day5)
    #You will seed each well with 100,000 cells according to the procedure demonstrated by the teaching faculty.
    12 KB (2,013 words) - 20:08, 30 September 2016
  • 20.109(F16):Generate gRNA plasmid (Day3)
    ...t from NEB to insert the gRNA sequence into an expression vector. For this procedure you will combine the gRNA primer you designed, a universal CRISPRi primer s ...ppropriate insertion (or 'mutation') was incorporated. The transformation procedure will be as follows:
    11 KB (1,747 words) - 20:42, 14 November 2016
  • 20.109(F16):Confirm gRNA sequence (Day5)
    The procedure for DNA isolation using small volumes is commonly termed "mini-prep," which ... gently, specifically '''kept cold and not vortexed'''. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
    14 KB (2,265 words) - 21:01, 10 November 2016
  • 20.109(F16):Asses DNA repair variability with comet chip (Day4)
    ...riables_%28Day2%29#Part_2:_Load_comet_chip M1D2]; however, please note the procedure for 'trapping' the cells into the microwells will be as follows: #Wash your CometChip using 1x PBS as done during the cell loading procedure.
    8 KB (1,390 words) - 17:23, 27 September 2016
  • 20.109(F16):Measure DNA damage with comet chip (Day3)
    You will load the CometChips you prepared on M1D1 using the procedure from [http://engineerbiology.org/wiki/20.109%28F16%29:Test_comet_chip_loadi *The procedure for 'trapping' the cells into the microwells will be as follows:
    11 KB (1,892 words) - 19:35, 26 September 2016
  • 20.109(F16): Research article
    ...e your audience is scientifically literate and somewhat familiar with each procedure. Remember that the methods should be divided into sub-sections that do not
    8 KB (1,243 words) - 16:42, 4 November 2016
  • 20.109(S17):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
    7 KB (1,195 words) - 15:51, 6 September 2017
  • 20.109(S17):Homework
    ... a way that can be followed by someone less familiar with the experimental procedure? Does the author use concentrations to convey amount?
    22 KB (3,470 words) - 18:41, 3 May 2018
  • 20.109(S17):Growth of phage materials (Day1)
    ... complete the next step in generating your nanowires. In this part of the procedure the phage-AuNP complexes will be biomineralized with FePO<sub>4</sub>. The
    16 KB (2,628 words) - 14:28, 24 April 2017
  • 20.109(S17):Battery assembly and testing (Day5)
    ...or negative current to charge or discharge the batteries, identical to the procedure used to test your battery. By attaching a battery to a galvanostat and appl ... away. Thank you, Jifa! Today you will see a demo of the battery assembly procedure. The steps are provided below for your notes.
    8 KB (1,391 words) - 14:18, 9 May 2017
  • 20.109(S17): Research article
    ...e your audience is scientifically literate and somewhat familiar with each procedure. Remember that the methods should be divided into sub-sections that do not
    8 KB (1,242 words) - 00:09, 4 April 2017
  • 20.109(S17):Evaluation of purified protein (Day3)
    ...PAGE to visualize the effectiveness of IPTG induction and the purification procedure.
    9 KB (1,496 words) - 21:33, 28 February 2017
  • Assignment 3, Part 1: visualizing actin with fluorescence contrast
    ==Fixing and staining procedure== In outline, the procedure is:
    15 KB (2,365 words) - 20:25, 25 February 2020
  • Creating 20.109(S17):Scan slides to identify FKBP12 binders (Day5)
    ... the source of the red fluorescent signal. Think back to the purification procedure. A His tag was used to isolate FKBP12 from the cell lysate. This tag can
    2 KB (324 words) - 17:43, 31 January 2017
  • 20.109(S17):Scan slides to identify FKBP12 binders (Day5)
    ... the source of the red fluorescent signal. Think back to the purification procedure. A His tag was used to isolate FKBP12 from the cell lysate. This tag can
    5 KB (810 words) - 19:49, 27 February 2017
  • 20.109(S17):Identify chemical structures common among FKBP12 binders
    ...eps that that did work as expected and how you would improve or change the procedure for 'better' results.
    5 KB (791 words) - 15:52, 6 March 2017
  • 20.109(S17):Confirm cell lines and practice tissue culture techniques (Day1)
    ...our cells, take the flask to your tissue culture hood to begin the seeding procedure. ... '''It is very important that you keep the cell lysate cold throughout the procedure.'''
    20 KB (3,359 words) - 21:06, 9 March 2017
  • 20.109(S17):Assess cell survival and harvest RNA for quantitative PCR assay (Day3)
    ...rbent paper with RNase Away and dry with a Kimwipe. Perform this cleaning procedure with all of the equipment you will need for the RNA purification protocol (
    13 KB (2,208 words) - 01:48, 9 February 2017
  • 20.109(S17):Prepare Western blot and induce DNA damage for quantitative PCR assay (Day2)
    After the electrophoresis procedure, the teaching faculty will assist you in assembling the transfer cassette a
    16 KB (2,444 words) - 17:15, 17 March 2017
  • 20.109(S17):Purify RNA for quantitative PCR assay and treat cells for survival assay (Day3)
    ...rbent paper with RNase Away and dry with a Kimwipe. Perform this cleaning procedure with all of the equipment you will need for the RNA purification protocol (
    15 KB (2,526 words) - 19:11, 30 October 2017
  • 20.109(F17):Homework
    ... a way that can be followed by someone less familiar with the experimental procedure? Does the author use concentrations to convey amount?
    21 KB (3,308 words) - 19:34, 27 October 2017
  • 20.109(F17):Module 1
    A video of the procedure can be found [http://www.jove.com/video/50607/cometchip-high-throughput-96-
    3 KB (473 words) - 16:31, 8 September 2017
  • 20.109(F17):Prepare comet chips (Day1)
    ...our cells, take the flask to your tissue culture hood to begin the seeding procedure.
    18 KB (2,935 words) - 20:42, 19 September 2017
  • 20.109(F17):Develop experiment to optimize loading variables (Day2)
    #'''Read through Steps #11-14 before continuing with the procedure.'''
    14 KB (2,296 words) - 14:28, 15 September 2017
  • Using nonlinear regression to create a camera specification sheet
    ...r confidence interval like this: Imagine that you repeated the measurement procedure precisely the same way 100 times. Because of random noise alone, the values
    30 KB (4,871 words) - 20:23, 29 August 2018
  • Assignment 5 Overview
    #Procedure
    3 KB (426 words) - 13:34, 23 March 2020
  • Assignment 1, Part 2: Optics bootcamp
    Once you are set up correctly, make a movie and plot the results using the procedure below:
    20 KB (3,371 words) - 06:40, 31 January 2020
  • Assignment 1, Part 3: Building your transillumination microscope
    ...nents, the manufacturer's website can be very helpful. For example, if the procedure calls for an SPW602 spanner wrench and you have no idea what such a thing m
    38 KB (6,482 words) - 19:23, 6 September 2019
  • Assignment 5, Part 2: live cell particle tracking of endocytosed beads
    #Procedure
    4 KB (742 words) - 18:01, 30 March 2020
  • Assignment 8 Overview
    #* Document the procedure used to gather your data with fluorescein or beater DNA:
    4 KB (615 words) - 18:43, 29 January 2018
  • Assignment 10 Overview
    Document your data collection procedure. Report instrument settings for each trial, including control software para
    8 KB (1,286 words) - 16:37, 8 May 2018
  • 20.109(F17):Generate gRNA plasmid (Day3)
    ...t from NEB to insert the gRNA sequence into an expression vector. For this procedure you will combine the gRNA primer you designed, a universal CRISPRi primer s ...ppropriate insertion (or 'mutation') was incorporated. The transformation procedure will be as follows:
    9 KB (1,451 words) - 16:32, 19 October 2017
  • 20.109(F17):Confirm gRNA sequence (Day5)
    The procedure for DNA isolation using small volumes is commonly termed "mini-prep," which ... gently, specifically '''kept cold and not vortexed'''. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
    14 KB (2,298 words) - 18:35, 27 October 2017
  • 20.109(F17):Growth of phage materials (Day1)
    ... complete the next step in generating your nanowires. In this part of the procedure the phage-AuNP complexes will be biomineralized with FePO<sub>4</sub>. The
    16 KB (2,693 words) - 16:31, 14 November 2017
  • 20.109(F17):Assemble and test batteries (Day5)
    ...or negative current to charge or discharge the batteries, identical to the procedure used to test your battery. By attaching a battery to a galvanostat and appl ... away. Thank you, Jifa! Today you will see a demo of the battery assembly procedure. The steps are provided below for your notes.
    8 KB (1,301 words) - 17:08, 5 December 2017
  • 20.109(F17):Evaluate cell loading results (Day3)
    ...y finish the second chip, the teaching faculty will finish the preparation procedure.
    8 KB (1,360 words) - 20:41, 19 September 2017
  • 20.109(F17):Test role of biochemical factors in genomic stability (Day4)
    You will use the following procedure to treat both of your CometChips. ...our CometChip using 1x PBS into the same dish used during the cell loading procedure.
    12 KB (1,986 words) - 21:55, 21 September 2017
  • 20.109(F17): Research article
    ...e your audience is scientifically literate and somewhat familiar with each procedure. Remember that the methods should be divided into sub-sections that do not
    8 KB (1,240 words) - 16:23, 15 November 2017
  • Assignment 2 Part 1: Noise in images
    ...rray'', containing data from a series of measurements made using a similar procedure to the one you used in assignment 1. <tt>DarkCurrentData</tt> contains meas
    24 KB (3,859 words) - 20:38, 18 February 2020
  • Assignment 4 part 2: Measure resolution
    ..., and divide by the magnification. In this part of the lab, you will use a procedure inspired by this simple idea to estimate the resolution of your microscope. #* This procedure is extremely sensitive to the focus adjustment.
    24 KB (3,552 words) - 19:42, 28 February 2020
  • 20.109(S18):Practice tissue culture techniques and prepare cells for RNA purification (Day1)
    ...our cells, take the flask to your tissue culture hood to begin the seeding procedure.
    13 KB (2,165 words) - 13:44, 20 March 2018
  • 20.109(S18):Evaluate protein purity and concentration (Day4)
    ...PAGE to visualize the effectiveness of IPTG induction and the purification procedure.
    12 KB (1,851 words) - 20:02, 26 February 2018
  • 20.109(S18):Induce DNA damage and apply drug treatments for RNA purification (Day2)
    ...a generated by the teaching faculty. You will, however, complete the same procedure used by the teaching faculty to prepare the RNA samples and then use your s ...ully read through the Culture Methods provided for these cells. Does this procedure differ from what you did in the previous laboratory session? How?
    7 KB (1,179 words) - 14:24, 14 March 2018
  • 20.109(S18): Research article
    ...e your audience is scientifically literate and somewhat familiar with each procedure. Remember that the methods should be divided into sub-sections that do not
    27 KB (4,131 words) - 18:31, 31 January 2018
  • 20.109(S18):Growth of phage-based active (cathode) material (Day1)
    ... complete the next step in generating your nanowires. In this part of the procedure the phage-AuNP complexes will be biomineralized with FePO<sub>4</sub>. The
    17 KB (2,773 words) - 13:17, 30 April 2018
  • 20.109(S18):Assemble and test batteries (Day5)
    ...or negative current to charge or discharge the batteries, identical to the procedure used to test your battery. By attaching a battery to a galvanostat and appl ... away. Thank you, Jifa! Today you will see a demo of the battery assembly procedure. The steps are provided below for your notes.
    8 KB (1,301 words) - 18:12, 9 May 2018
  • 20.109(S18):Purify RNA and practice RNA-seq data analysis methods (Day3)
    ...rbent paper with RNase Away and dry with a Kimwipe. Perform this cleaning procedure with all of the equipment you will need for the RNA purification protocol (
    12 KB (1,963 words) - 00:38, 19 March 2019
  • 20.109(S18):Homework
    ... a way that can be followed by someone less familiar with the experimental procedure? Does the author use concentrations to convey amount?
    23 KB (3,594 words) - 18:41, 3 May 2018
  • 20.109(S18): M1 Data Summary
    *'''Figure''': Simplified schematic of SMM procedure.
    9 KB (1,376 words) - 16:05, 10 March 2018
  • 20.109(F18):Homework
    ... a way that can be followed by someone less familiar with the experimental procedure? Does the author use concentrations to convey amount?
    22 KB (3,505 words) - 21:06, 21 August 2019
  • 20.109(F18):Module 1
    *A video of the procedure is linked [http://www.jove.com/video/50607/cometchip-high-throughput-96-wel
    5 KB (776 words) - 01:21, 21 September 2018
  • 20.109(F18):Generate gRNA plasmid (Day3)
    ...t from NEB to insert the gRNA sequence into an expression vector. For this procedure you will combine the gRNA primer you designed, a universal CRISPRi primer s ...ppropriate insertion (or 'mutation') was incorporated. The transformation procedure will be as follows:
    9 KB (1,441 words) - 20:11, 15 October 2018
  • 20.109(F18):Confirm gRNA sequence (Day5)
    The procedure for DNA isolation using small volumes is commonly termed "mini-prep," which ... gently, specifically '''kept cold and not vortexed'''. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
    14 KB (2,323 words) - 18:26, 25 October 2018
  • 20.109(F18):Growth of phage-based active (cathode) material (Day1)
    ... complete the next step in generating your nanowires. In this part of the procedure the phage-AuNP complexes will be biomineralized with FePO<sub>4</sub>. The
    16 KB (2,742 words) - 18:15, 13 November 2018
  • 20.109(F18):Assemble and test batteries (Day5)
    ...or negative current to charge or discharge the batteries, identical to the procedure used to test your battery. By attaching a battery to a galvanostat and appl ... away. Thank you, Jifa! Today you will see a demo of the battery assembly procedure. The steps are provided below for your notes.
    8 KB (1,303 words) - 20:24, 4 December 2018
  • 20.109(F18): Research article
    ...e your audience is scientifically literate and somewhat familiar with each procedure. Remember that the methods should be divided into sub-sections that do not
    27 KB (4,087 words) - 01:33, 8 November 2018
  • 20.109(F18):Practice tissue culture and prepare microwell array (Day1)
    ...our cells, take the flask to your tissue culture hood to begin the seeding procedure.
    19 KB (3,096 words) - 18:35, 26 September 2018
  • 20.109(F18):Prepare and treat cells for genomic instability experiment (Day3)
    ...our CometChip using 1x PBS into the same dish used during the cell loading procedure.
    9 KB (1,587 words) - 22:12, 20 September 2018
  • 20.109(F18):Design cell loading optimization experiment and discuss genomic instability experiment (Day2)
    #'''Read through Steps #11-14 before continuing with the procedure.'''
    16 KB (2,668 words) - 17:38, 25 September 2018
  • Assignment 5, Part 1: MSD difference tracking and microscope stability
    Once you have developed and tested your code, use the following procedure to measure the location precision of your microscope:
    9 KB (1,018 words) - 19:38, 1 April 2020
  • 20.109(S19):Homework
    ... a way that can be followed by someone less familiar with the experimental procedure? Does the author use concentrations to convey amount?
    24 KB (3,715 words) - 01:02, 30 April 2019
  • 20.109(S19):Growth of phage-based active (cathode) material (Day1)
    ... complete the next step in generating your nanowires. In this part of the procedure the phage-AuNP complexes will be biomineralized with FePO<sub>4</sub>. The
    16 KB (2,740 words) - 16:36, 18 April 2019
  • Exam 2 study guide
    ** The fitting procedure requires specifying the form of the model, i.e. the order of the numerator
    16 KB (2,514 words) - 12:16, 9 May 2019
  • 20.109(S19):Assemble and test batteries (Day5)
    ...or negative current to charge or discharge the batteries, identical to the procedure used to test your battery. By attaching a battery to a galvanostat and appl ... away. Thank you, Jifa! Today you will see a demo of the battery assembly procedure. The steps are provided below for your notes.
    8 KB (1,299 words) - 17:49, 7 May 2019
  • 20.109(S19): Data Summary
    *'''Figure''': Simplified schematic of SMM procedure.
    10 KB (1,422 words) - 14:19, 12 March 2019
  • 20.109(S19):Purify RNA and practice RNA-seq data analysis methods (Day3)
    ...rbent paper with RNase Away and dry with a Kimwipe. Perform this cleaning procedure with all of the equipment you will need for the RNA purification protocol (
    12 KB (1,923 words) - 20:29, 16 April 2019
  • 20.109(S19):Evaluate protein purity and concentration (Day4)
    ...PAGE to visualize the effectiveness of IPTG induction and the purification procedure.
    12 KB (1,951 words) - 14:41, 22 February 2019
  • 20.109(S19):Practice tissue culture techniques and prepare cells for RNA purification (Day1)
    ...our cells, take the flask to your tissue culture hood to begin the seeding procedure. ...ully read through the Culture Methods provided for these cells. Does this procedure differ from what you did in the previous laboratory session? How?
    14 KB (2,344 words) - 20:32, 16 April 2019
  • 20.109(S19):Etoposide treat cells for RNA purification (Day2)
    ...a generated by the teaching faculty. You will, however, complete the same procedure used by the teaching faculty to prepare the RNA samples and then use your s
    6 KB (972 words) - 16:16, 19 March 2019
  • 20.109(S19):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
    7 KB (1,195 words) - 23:28, 6 March 2019
  • 20.109(S19): Research article
    ...e your audience is scientifically literate and somewhat familiar with each procedure. Remember that the methods should be divided into sub-sections that do not
    27 KB (4,062 words) - 19:40, 19 April 2019
  • 20.109(F19):Practice cell culture and begin sub-nuclear foci assay (Day1)
    ...onstruct a CometChip; however, it is best to familiarize yourself with the procedure and assay before then. ...our cells, take the flask to your tissue culture hood to begin the seeding procedure.
    14 KB (2,233 words) - 16:30, 9 September 2019
  • 20.109(F19):Generate gRNA plasmid (Day3)
    ...t from NEB to insert the gRNA sequence into an expression vector. For this procedure you will combine the gRNA primer you designed, a universal CRISPRi primer s ...ppropriate insertion (or 'mutation') was incorporated. The transformation procedure will be as follows:
    9 KB (1,444 words) - 20:44, 18 September 2019
  • 20.109(F19):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
    7 KB (1,197 words) - 18:28, 14 August 2019
  • 20.109(F19):Treat cells for gamma-H2AX (Day2)
    ...ther table to assist you in preparing the mixes needed in this step of the procedure.
    10 KB (1,755 words) - 19:12, 17 September 2019
  • 20.109(F19):Confirm gRNA sequence in pgRNA(Day5)
    The procedure for DNA isolation using small volumes is commonly termed "mini-prep," which ... gently, specifically '''kept cold and not vortexed'''. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
    14 KB (2,323 words) - 19:11, 14 August 2019
  • 20.109(F19):Confirm gRNA sequence in pgRNA (Day5)
    The procedure for DNA isolation using small volumes is commonly termed "mini-prep," which ... gently, specifically '''kept cold and not vortexed'''. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
    15 KB (2,355 words) - 17:02, 25 October 2019
  • 20.109(F19):Homework
    ... a way that can be followed by someone less familiar with the experimental procedure? Does the author use concentrations to convey amount?
    22 KB (3,438 words) - 17:37, 16 October 2019
  • 20.109(F19):Module 1
    *A video of the procedure is linked [http://www.jove.com/video/50607/cometchip-high-throughput-96-wel
    6 KB (928 words) - 22:27, 10 September 2019
  • 20.109(F19): Research article
    ...e your audience is scientifically literate and somewhat familiar with each procedure. Remember that the methods should be divided into sub-sections that do not
    27 KB (4,080 words) - 23:37, 11 November 2019
  • 20.109(F19):Complete gamma-H2AX assay and prepare CometChip (Day3)
    #'''Read through Steps #11-14 before continuing with the procedure.'''
    16 KB (2,626 words) - 20:47, 25 September 2019
  • 20.109(F19):Load cells into CometChip and apply treatments (Day4)
    ...our CometChip using 1x PBS into the same dish used during the cell loading procedure.
    12 KB (1,976 words) - 15:14, 24 September 2019
  • Laser cutter safe operating procedure
    ==Cutting procedure==
    9 KB (1,503 words) - 19:03, 15 December 2019
  • 20.109(F19):Prepare for cellular thermal shift assay (Day1)
    ...our cells, take the flask to your tissue culture hood to begin the seeding procedure.
    14 KB (2,315 words) - 16:18, 7 November 2019
  • 20.109(F19):Incubate with ligand and apply heat treatment for protein denaturation (Day2)
    #*Complete the snap freeze procedure a total of three times; however after the third snap freeze, place the tube
    6 KB (994 words) - 19:04, 15 November 2019
  • 20.109(S20):Homework
    ... a way that can be followed by someone less familiar with the experimental procedure? Does the author use concentrations to convey amount?
    24 KB (3,726 words) - 16:31, 25 April 2020
  • 20.109(S20):Assess purity and concentration of TDP43 protein (Day3)
    ...sualize the effectiveness of IPTG/arabinose induction and the purification procedure.
    12 KB (1,919 words) - 19:18, 27 February 2020
  • 20.109(S20):Prepare cells for RNA purification and practice RNA-seq data analysis methods (Day1)
    ...our cells, take the flask to your tissue culture hood to begin the seeding procedure.
    13 KB (2,111 words) - 21:39, 1 March 2020
  • 20.109(S20):Treat cells with etoposide and analyze RNA-seq data (Day2)
    ...a generated by the teaching faculty. You will, however, complete the same procedure used by the teaching faculty to prepare the RNA samples and then use your s
    4 KB (621 words) - 19:56, 10 March 2020
  • 20.109(S20):Purify RNA from etoposide-treated cells and and generate cDNA (Day3)
    ...rbent paper with RNase Away and dry with a Kimwipe. Perform this cleaning procedure with all of the equipment you will need for the RNA purification protocol (
    10 KB (1,734 words) - 20:59, 28 February 2020
  • 20.109(S20):Examine TDP43 binders for chemical features (Day7)
    ...eps that that did work as expected and how you would improve or change the procedure for 'better' results.
    5 KB (754 words) - 19:01, 2 March 2020
  • 20.109(S20):Enrich candidate clones using fluorescence-activated cell sorter (FACS) (Day1)
    ...hine is only able to count, and not sort subpopulations of cells, then the procedure is called flow cytometry, while cell sorting is called FACS.
    9 KB (1,437 words) - 18:28, 14 April 2020
  • 20.109(S20):Harvest candidate clones (Day2)
    ... are prepared to take up DNA they are called competent. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10< Note: The procedure for DNA isolation at this scale (1-2mL) is commonly termed "mini-prep," whi
    13 KB (2,077 words) - 13:29, 16 April 2020
  • 20.109(S20):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
    7 KB (1,195 words) - 20:10, 28 February 2020
  • Assignment 5: Spring 2020
    #* You do not need to document your cell-preparation procedure since you didn't get a chance to do it!
    705 B (100 words) - 20:05, 20 March 2020

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