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Create the page "Needs Work" on this wiki!
- ...as some shortcomings, it will give you a good idea of how all of the parts work together to make melting curves. The instrument includes a resistance tempe ...his lab. You will probably notice some shortcomings your instrument as you work. Don't worry about that — in part 2, you will make many improvements27 KB (4,497 words) - 17:49, 19 April 2017
- ...gher frequency. So rip that sucker off — now your amplifier needs to work on a signal that is changing much more quickly. ...ou may need to update the gains of one or both of your amplifier stages to work best with the lower signal level and increased frequency.20 KB (3,227 words) - 20:05, 7 November 2017
- ... at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of11 KB (1,661 words) - 19:53, 10 May 2017
- ...th state variables. Because of this assumption, lumped element models only work well if the bodies you are modeling have a nearly uniform temperature. Comp18 KB (2,803 words) - 21:55, 17 August 2017
- ... at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of2 KB (353 words) - 16:25, 27 December 2016
- ...gher frequency. So rip that sucker off — now your amplifier needs to work on a signal that is changing much more quickly. ...ou may need to update the gains of one or both of your amplifier stages to work best with the lower signal level and increased frequency.17 KB (2,813 words) - 22:56, 7 November 2017
- ...your apparatus with sufficient detail for another person to replicate your work. Assume the reader is familiar with the concepts of 20.309 and has access t6 KB (864 words) - 18:50, 16 April 2017
- ===Hi! I work, even though there's no writeup!===12 KB (1,459 words) - 10:25, 24 May 2011
- ... in the lab. The work is divided into 3 parts. Each part requires some lab work, some analysis, lots of clear thinking, and a written report. You will subm23 KB (3,767 words) - 15:19, 10 January 2017
- ...iments of J. Perrin that lead to his Nobel Prize. A translation of the key work is included in the reprints folder. Have a look – he used latex spheres, ...a [http://en.wikipedia.org/wiki/Motor_protein motor protein] that does the work. The motor molecule uses energy from the hydrolysis of one ATP molecule to15 KB (2,322 words) - 17:14, 18 September 2012
- ...he plus terminal to the minus terminal. In most of the components you will work with, such as wires, capacitors, and resistors, the charge carriers are neg22 KB (3,534 words) - 13:50, 19 October 2018
- Some lab exercises involve significant design and construction work. Others utilize complex systems that were built by others. In either case, ...y with other students. '''''The report you submit must consist entirely of work of done by members of your lab group. Any words, data, images, code, or oth10 KB (1,534 words) - 21:33, 13 March 2017
- [[Category:Needs review]] ...ses can be completed in about 6 hours per week. Some students find the lab work significantly more or less time consuming. If you have little experience bu5 KB (714 words) - 16:37, 7 February 2020
- ...Also, the blinking laser safety sign near the door must be turned any time work with these lasers is taking place. Needless to say, purposefully aiming the ...risk of electrical shock from the low voltage electrical circuits you will work with in 20.309.5 KB (821 words) - 16:50, 10 September 2015
- h. Demonstrate conscientious work habits5 KB (658 words) - 02:45, 7 July 2010
- #Work with a TA or Instructor. To be useful for imaging, an AFM needs to scan its probe over the sample surface. Our microscopes are designed wit56 KB (9,320 words) - 20:42, 13 December 2017
- As evidenced by Nagai’s work, wild-type inverse pericam is not toxic to BL21(DE3)pLysS cells. Although i ...u induce your cells with IPTG, you will let the protein factories do their work for 2-3 hours. During this time, you will evaluate the DNA from your two X#16 KB (2,609 words) - 21:55, 23 February 2016
- ...ve for the trap stiffness, the variance in position is the only thing that needs to be calculated. Since this variance is measured in volts, position calibr ...so TransferFunc would be saved as TransferFunc.m. Otherwise, they will not work).:24 KB (4,010 words) - 15:32, 5 August 2010
- Draw a ray diagram to justify your answer. A ruler will help keep your work tidy. (Hint: will any of the rays stay the same?) ...ems do you think the fish uses for underwater vision? Why does this system work better below water? Draw a few rays and/or include a couple of bullet point4 KB (740 words) - 02:53, 15 September 2010
- ... glancing angle, using the bench ligths for illuination. If this does not work, ask a TA or Instructor to help you.5 KB (878 words) - 23:26, 5 October 2010
- ...ity to pursue a specific technique in depth. You will have many chances to work on your writing, presentation, and project planning skills. Active particip ...Developing an entirely new in vitro neuronal guidance technology is folly. Work closely with your mentor to find the right balance. The lab has a modest bu11 KB (1,722 words) - 17:22, 6 February 2018
- ...uld detail what you are planning to do, the motivation for doing it, prior work, and relevant theory. It should include a comprehensive development plan an ...m line on motivation, goal, key milestones, risks, resources, and previous work.2 KB (283 words) - 00:40, 12 February 2014
- *we have little electronics experience, and Jenn needs an electronics review :) ****this didn't work either because the polymer is too hydrophobic or it is blocked4 KB (610 words) - 00:07, 3 April 2011
- ...ize the current needed to heat the wire, while still being thick enough to work with. The thermal epoxy ensures that the resistance wire does not make cont We definitely see less smearing with newer version. There is of course much work still to do.8 KB (1,345 words) - 19:36, 19 May 2011
- * ordered parts for heated lid may not work, or may not come in for a while * PCR may not work even with heated lid - will need to think of other ways to fix this problem3 KB (447 words) - 04:09, 8 April 2011
- - Work will start with the image analysis programming, including the analysis of c ...on seems to be adapting the QuickPALM code available in open source to our needs9 KB (1,454 words) - 20:19, 19 May 2011
- * needs Power of .5W (12V-DC, 0.04 Amps) ... precise control of the fluid (chemoeffector band width and stoppped flow) needs to be improved.14 KB (2,196 words) - 23:14, 18 May 2011
- If you prefer to work in Python, please see [[Python:Simulating DNA Melting|this page]]. Does the <tt>DnaFraction</tt> function work as expected? A good way to test the function is to plot a melting curve gen13 KB (2,026 words) - 22:46, 15 April 2018
- Work performed and risks identified along the way.519 B (60 words) - 19:38, 19 May 2011
- ===Work Performed===125 B (15 words) - 16:51, 30 March 2011
- ...nd silicone tape sandwiched between two plates of aluminum...but it didn't work too well. They said it "smoked and burned out." However, they were designin3 KB (571 words) - 19:07, 4 April 2011
- ...hernet Controller]. We now have control via RS232 (couldn't get TCP/IP to work)925 B (132 words) - 20:52, 15 May 2011
- This page is designed to be useful for whoever is just starting to work on the 20.309/345 QRT-PCR machine.901 B (144 words) - 23:03, 4 May 2011
- Also attempted to pour some agar plugs which didn't work out very well.792 B (113 words) - 03:32, 15 May 2011
- ===Work=== ===Work===5 KB (849 words) - 08:32, 19 May 2011
- ...roke. The lid is not hooked up, but it is ready. I wish I had been able to work faster and do more, but... I'll be back tomorrow. ...iew code. I will be working with versions 2.700 and later. Not all of them work. I will take notes on this; take a look at [[QRT-PCR:LabView-versions]].7 KB (1,228 words) - 16:48, 19 May 2011
- ... confocal microscope in the 20.309 laboratory. Stephanie Bachar continued work on the microscope during January of 2011. When beginning our project, the21 KB (3,664 words) - 21:38, 4 June 2015
- ... the camera in each photoswitching cycle. This calculation is based on the work of Thompson et al. where a detailed analysis of single molecule localizatio9 KB (1,442 words) - 20:08, 19 May 2011
- ... After tapping the screwdriver into the groove, the cover would gradually work its way off, though cracks would occur in the plastic around the top edge.7 KB (1,060 words) - 13:29, 19 May 2011
- ...er for us to use? (Kaduk says that a nonpolar polymer gel-like thing might work a lot better than trying to find glycerol-plus.) Of course, diffusion is ab A thought about the glycerol thing -- why doesn't this work with gels? Or does it? Viscosity is not the same as gelification, I suppose11 KB (1,755 words) - 23:31, 18 July 2011
- Below is a list of the most relevant references used for this work:2 KB (233 words) - 20:37, 19 May 2011
- '''Getting it to work with the old photodiode''' ... of code. TODO: see if fitting can be sped up. TODO: see if animation will work on Athena or 309 lab computers.11 KB (1,864 words) - 21:20, 22 July 2011
- ...ith electronics and electrical measurement equipment, you may only need to work through a few of the sections in the lab manual. However, you will at least1 KB (236 words) - 18:58, 13 August 2013
- ...rything in your report, including computer code, must be entirely your own work.'''''2 KB (363 words) - 20:20, 10 April 2014
- Diligent record keeping is a vital component of superior lab work. For hundreds of years, lab notebooks looked like like the example at righ ...here proactive or responsive things you should do differently, to maximize work with others?4 KB (610 words) - 18:44, 13 August 2013
- ... to instrumentation that make the difference in enabling researchers to do work that would otherwise be outside of their budget. ** If this doesn't work, restart Matlab3 KB (464 words) - 21:52, 27 February 2012
- ...a is one example, taken from tethered bead data at 20 mW. This method will work best with a tethered bead stuck in the trap at an oscillation amplitude > t13 KB (2,020 words) - 19:56, 9 March 2012
- ...ect such info in the current wiki page. In particular, the ImageJ tutorial needs to be corrected.680 B (90 words) - 18:33, 15 August 2013
- ...here proactive or responsive things you should do differently, to maximize work with others? ...s definitely affect my work ethic. I can be a quick learner, I can do good work, but if my heart's not in it, quality tanks.14 KB (2,366 words) - 04:50, 17 May 2012
- I have decided to work with SLM's for my final project because they are extremely useful devices t7 KB (1,162 words) - 07:22, 14 March 2012
- This project is restricted to nine weeks of work and experimentation. We have set aside three weeks for experimentation, wit ...mportant milestone, since it allows focusing work on getting the imager to work with software in the upcoming weeks.14 KB (2,166 words) - 05:15, 18 May 2012
- This week, I have been '''trying''' to modify LFDisplay to work with hexagonal grids, but the code is difficult to understand and I suspect ... optics that disappeared when the optics disappeared. Now, this just means work as hard as possible now that the optics are off.8 KB (1,373 words) - 18:43, 13 August 2013
- I began a tiny bit of work on movement of the stage for the center determining code. Started to work on PSF lab. There was a bug in the code, and the stage would not move. Will11 KB (1,937 words) - 22:57, 22 May 2012
- * Learning how optical components like those from ThorLabs come together and work.4 KB (611 words) - 21:37, 4 June 2015
- Wiki Page for Real Time qPCR work154 B (15 words) - 21:13, 15 May 2012
- I would be thrilled to work on this project for a number of reasons: * It will force me to work on image processing, I am novice status :p3 KB (368 words) - 15:41, 17 May 2012
- I would be thrilled to work on this project for a number of reasons: * It will force me to work on image processing, I am novice status :p4 KB (576 words) - 05:19, 18 May 2012
- I would be thrilled to work on this project for a number of reasons: * It will force me to work on image processing, I am novice status :p2 KB (335 words) - 15:59, 17 May 2012
- === Prior Work === ...f>, his thesis<ref>[http://www.lytro.com/renng-thesis.pdf]</ref>, and some work from a postdoc at Harvard<ref>[http://rubenportugues.net/me.shtml]</ref>. T18 KB (2,796 words) - 05:15, 18 May 2012
- ...i contact info and the Bio-Instrumentation research labs or companies they work at. Use these sages to help you learn how 20.309 curriculum and skills are583 B (81 words) - 07:32, 6 December 2013
- ...iments of J. Perrin that lead to his Nobel Prize. A translation of the key work is included in the reprints folder. Have a look – he used latex spheres,9 KB (1,469 words) - 01:01, 10 March 2016
- ** Try not to disturb air flow, yet still work! * Work 6" inward, away from glass.18 KB (2,896 words) - 18:17, 25 February 2024
- ...even after moving your microscope. Understanding how all of the components work together can be daunting. Ask about any components that perplex you. <li> One way to make this work is to rescale your image to 16 bits: <tt> imshow( 65535/4095 * microruler1023 KB (3,758 words) - 22:45, 12 January 2017
- ...iments of J. Perrin that lead to his Nobel Prize. A translation of the key work is included in the reprints folder. Have a look – he used latex spheres,7 KB (1,071 words) - 01:05, 13 September 2012
- * How does 20.309 apply to your work? * Should I get PhD/MS or work at a company?781 B (105 words) - 01:19, 6 December 2013
- ... laser with focusing optics. You must attend the safety lecture before you work with the laser. See an instructor if you missed the lecture. Do not begin w When you work with lasers, keep these laser safety best practices in mind:20 KB (3,262 words) - 22:06, 14 August 2017
- ...ram again, and/or close it, re-open it, and run it again. If this does not work, consult a TA or Instructor.4 KB (644 words) - 21:08, 29 August 2013
- ...ram again, and/or close it, re-open it, and run it again. If this does not work, consult a TA or Instructor.9 KB (1,422 words) - 23:19, 19 November 2013
- ...he image plane since there is no place the rays actually come together; it needs another lens (such as your eye) in order to view it. More examples of real ... image. Although the image is inverted on your retina, your brain does the work for you to see the image in the right orientation.23 KB (3,769 words) - 01:20, 27 August 2019
- ...l = 0.5 * ( max( ImageData(:) ) + median( ImageData(:) ) ); % this may not work in all cases17 KB (1,769 words) - 14:21, 30 September 2017
- ...uwenhoek and Robert Hooke made dramatic improvements in microscopes. Their work brought about many fundamental discoveries. Both men documented the existen * How does the probe work? What properties or events does it measure?15 KB (2,262 words) - 14:22, 6 September 2018
- ...banana terminals in place. On new breadboards these are held away from the work surface by rubber or plastic feet on the backboard. On our breadboards thes9 KB (1,443 words) - 16:28, 20 October 2015
- ...l. Fortunately, there always seems to be somebody with a bigger office who needs a couch. ...ch time you repeat a measurement, the incremental value added by your hard work diminishes. The tenth measurement is only about 11% as valuable as the firs24 KB (3,757 words) - 14:57, 3 September 2018
- ...he work of Mason ''et al.'' to 3T3 cells in particular can be found in the work of Tseng ''et al.'' <ref>[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC130235 KB (750 words) - 18:34, 18 October 2013
- ...ram again, and/or close it, re-open it, and run it again. If this does not work, consult an Instructor.10 KB (1,467 words) - 00:16, 8 November 2017
- ...d/or close MATLAB, re-open it, and run BasicMelter again. If this does not work, consult a TA or Instructor.5 KB (719 words) - 23:46, 7 November 2017
- ...some exercises in the lab. The written problems will help you with the lab work. After you do the problems, come on by the lab. (Or do the problems in lab ... ray tracing and Snell's law to locate the image of the turtle. Show your work. <br>28 KB (4,857 words) - 20:09, 31 July 2017
- ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of3 KB (406 words) - 14:24, 5 June 2015
- #None: all work today is computer work. #*As before, ~1:20 dilution initiated between 8:30 and 9:30 am should work well.8 KB (1,223 words) - 16:04, 15 June 2015
- '''Prep Work''' *NB168 pCX-D3G+Linker -- This plasmid needs to be cut in three ways and each bkb purified!12 KB (1,868 words) - 16:04, 15 June 2015
- ...of Block Buffer to the plate. Wait another 60-90 minutes. In the meantime, work on your analysis (Part 3).14 KB (2,255 words) - 16:04, 15 June 2015
- ...of your labmates time.''' Reading the protocol in advance will help you work more quickly, and is strongly recommended. ...ls can basically be treated the same way. You and your partner may want to work in parallel, one on 2D prep and one on 3D prep. Unless otherwise stated, al18 KB (3,087 words) - 16:04, 15 June 2015
- ... has 15 μL of liquid in it. You might use the table below to guide your work. #Measure the absorbance of each sample at 595 nm. Work as quickly as you can, because the absorbance will continue to slowly chang12 KB (1,946 words) - 16:04, 15 June 2015
- ... of the surrounding environment on cell phenotype. In particular, you will work with primary chondrocytes in both liquid and gel culture. The specific aspe ...many cells you need to initiate your cultures, in case extra animal tissue needs to be ordered. Per 3D sample, you will prepare 1 mL of alginate beads (thus5 KB (805 words) - 16:04, 15 June 2015
- ...eir knee joints is more similar to that of humans. In this module, we will work with an ''in vitro'' culture model of cartilage-forming cells. ... time however you find useful (FNT assignment, notebook prep, or unrelated work).12 KB (2,006 words) - 16:04, 15 June 2015
- ...eting your cells, clean your microfuge. You can finish setting up your RNA work area while the cells spin down. ... different from DNA in its stability. Consequently it is more difficult to work with RNA in the lab. It is not the techniques themselves that are difficult16 KB (2,751 words) - 16:04, 15 June 2015
- ...king about the structure of cartilage for a bit, as promised on Day 4. Our work in this module has focused on chondrocytes themselves (viability and morpho ... diluted antibody per well. Incubate for 90 min (at room temperature), and work on Part 2 of today's protocol.9 KB (1,409 words) - 16:04, 15 June 2015
- ...nce they will undergo partly different manipulations. You may also want to work on two separate worksheets, one for each collagen type.7 KB (1,208 words) - 16:04, 15 June 2015
- ... semester in lab, but you will <font color = red>submit individual written work (lab report, portfolio, essay) and give individual journal club presentatio ...in the "for next time" section of each lab day. <font color = red> You can work with your lab partner, friends and teaching assistants on these assignments3 KB (394 words) - 14:24, 5 June 2015
- ...figures, but if you do so, you must include the reference to the published work (e.g., the figure should not be downloaded from a web site). ...re used to detect homologous recombination in mammalian cells. The reader needs to know what the goal is: which plasmid did you set out to create? Part B w10 KB (1,670 words) - 14:24, 5 June 2015
- ...pful discussions during the development of this module, as well as for her work in developing a [http://openwetware.org/wiki/20.109:Module_2 related module3 KB (445 words) - 14:24, 5 June 2015
- ... establish good habits for documentation of your work. By documenting your work according to the exercises done today, you will ...re there are no spaces between or after the letters or your search won’t work. Change the color of the start codon to blue. Repeat the “Find…” proc27 KB (3,752 words) - 14:24, 5 June 2015
- We gratefully acknowledge 20.109 instructor Natalie Kuldell for her crucial work developing and teaching previous iterations of this module.4 KB (637 words) - 14:24, 5 June 2015
- Ligations generally work best when there is a 1:4 ratio of backbone to insert. You will ligate your10 KB (1,806 words) - 14:24, 5 June 2015
- *work best in the same buffer and at the same temperature.<br> ...faculty has set up four overnight LB + AMP cultures of bacteria for you to work with. Three of these cultures have colonies from the ligation reactions you10 KB (1,634 words) - 14:24, 5 June 2015
- ==Sign your work==3 KB (494 words) - 14:24, 5 June 2015
- ...s wasting a month of your time repeating experiments already proven not to work or reinventing the wheel. ...erse pericam (IPC). You will annotate these sequences to guide your design work.15 KB (2,452 words) - 14:24, 5 June 2015
- ...al of information in order to design mutagenized inverse pericams nice work! Today you will put your designs into practice. ...reaction, to ensure that all the reagents are working properly. You should work quickly but carefully, and keep your tubes in a chilled container at all ti12 KB (1,954 words) - 14:24, 5 June 2015
- #Incubate on ice for 1 hour. (You might work on parts 3-5 of today's protocols now.) ===Microbial work===13 KB (2,221 words) - 14:24, 5 June 2015
- ... your partner and to others in the lab if you are not up to speed when the work begins. ... have cut yourself (even if you consider it minor), if something broke and needs cleaning up, or if you are on fire.2 KB (376 words) - 14:24, 5 June 2015
- *Procedures for doing tissue culture work.903 B (119 words) - 14:24, 5 June 2015
- ...f your own strengths and weaknesses can often help you improve your future work. After you give your presentation today, write a brief self-evaluation. Spe9 KB (1,472 words) - 14:24, 5 June 2015
- Our lab is beautifully equipped. We have a fume hood for work that generates hazardous vapors. We have a back room where dishes can be wa22 KB (3,631 words) - 14:24, 5 June 2015
- As evidenced by Nagai s work, wild-type inverse pericam is not toxic to BL21(DE3) cells. Although it is ...our cells with IPTG, you will let the resultant protein factories do their work for 2-3 hours. During this time, you will look at the sequencing results fo15 KB (2,475 words) - 14:25, 5 June 2015
- ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.5 KB (761 words) - 14:25, 5 June 2015
- ... list the name of your partner on your report since she contributed to the work. ... trends, raw data (like a picture of a gel), or a model that explains your work.12 KB (1,912 words) - 14:25, 5 June 2015
- ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.7 KB (1,095 words) - 14:25, 5 June 2015
- ...got last time. Begin by analyzing the wild-type protein as a check on your work (your curve should resemble Nagai's Figure 3L), then move on to your mutant ...SingleKD]], and [[Media:Fit_KDn.m| Fit_KDn]]. Move them to the C:/MATLAB72/work folder on your PC.14 KB (2,336 words) - 14:25, 5 June 2015
- ...lt projects should be tackled with the idea that they can, should and will work. Attitude alone, however, does not overcome pitfalls and there is much to b ...distinguish these possible explanations but it can tell you that something needs to be re-worked.19 KB (3,251 words) - 15:32, 15 June 2015
- ... your partner and to others in the lab if you are not up to speed when the work begins. ... have cut yourself (even if you consider it minor), if something broke and needs cleaning up, or if you are on fire.2 KB (380 words) - 15:32, 15 June 2015
- Our lab is beautifully equipped. We have a fume hood for work that generates hazardous vapors. We have a back room where dishes can be wa22 KB (3,724 words) - 15:32, 15 June 2015
- ...ake a figure using the data you have collected. <font color = red> You can work with your lab partner and friends on these assignments but you will hand in <font color= red><b>Late work will be penalized 1/3 of a letter grade for each day late and will not be a2 KB (279 words) - 15:32, 15 June 2015
- ...gulating protein stability, localization and modifications. Thus, from the work we've done so far this term, you may have the idea that gene expression is ...tocol for designing the reverse primer is just one method of many that can work. For example, taking the reverse complement can be done at any stage, makin28 KB (4,311 words) - 15:32, 15 June 2015
- #* needed resources to complete the work (1 slide) ... vary depending on the particulars of your proposal. You will have time to work on the presentation in lab next time as well as ask questions about the for5 KB (887 words) - 15:32, 15 June 2015
- You are asked to write a formal lab report detailing your work in this module. Specifics for each section of this report are detailed belo *conclusions you can draw from your work, including any uncertainties3 KB (479 words) - 15:32, 15 June 2015
- When you have a break from the work described below, be sure to examine the plaques you plated last time. Recor ...assay, what is the titer of each stock solution of phage? Please show your work. If the plaques appeared different, please consider how the phage genomes d12 KB (2,079 words) - 15:32, 15 June 2015
- ==Sign your work==3 KB (497 words) - 15:32, 15 June 2015
- ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.4 KB (732 words) - 15:32, 15 June 2015
- ...el should take approximately one hour to run. During that hour, you should work on parts two and three of today's protocol.<br>11 KB (1,756 words) - 15:32, 15 June 2015
- ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary. ...n the hood and maintain plenty of clear space in the center of the hood to work in.6 KB (1,050 words) - 15:32, 15 June 2015
- ... list the name of your partner on your report since she contributed to the work. ... trends, raw data (like a picture of a gel), or a model that explains your work.12 KB (1,849 words) - 15:32, 15 June 2015
- ...lls is extremely low (~10<sup>-7</sup>M). Calcium pumps and gated channels work hard to establish and keep this steep gradient. Inside the cell, calcium is ...range of such techniques. <b> If you remember only one detail from today's work, let it be the importance of choosing an appropriate tool for the measureme19 KB (3,069 words) - 15:48, 15 June 2015
- ...ng so, you may be switching SAGA-subunits and you will have to rethink the work you have planned so far. And if applicable, it will be worth noting the "ne ...review article] that was written to celebrate and highlight this important work on the structure of the SAGA complex. You and your partner will be randomly9 KB (1,466 words) - 15:32, 15 June 2015
- # Choose one of the MDS collection to work with, describing in your lab notebook the deletions that the strain include4 KB (600 words) - 15:32, 15 June 2015
- ...polymerases, this enzyme can only add sequence to an existing chain and so needs a short “primer” to begin synthesis. To perform the cDNA synthesis, you ...NA only” pipet tips and solutions and wiping down your bench. You should work on a fresh piece of benchpaper and remember to wear gloves when working wit17 KB (2,874 words) - 15:32, 15 June 2015
- ...ling) can be applied as a design tool for biological engineering. Your lab work with M13 will provide the context for your argument. ...erested in it. To do that, you need to describe the larger context for the work, the ways it’s important, and the specific areas your paper will address.7 KB (1,122 words) - 15:32, 15 June 2015
- #* needed resources to complete the work (1 slide) ... vary depending on the particulars of your proposal. You will have time to work on the presentation in lab next time as well as ask questions about the for2 KB (319 words) - 15:32, 15 June 2015
- Today you will work with a yeast strain bearing either the original pCT-CON plasmid, that expre Some of the work for this experiment has already been done for you. Two nights ago, the yeas9 KB (1,473 words) - 15:31, 15 June 2015
- ...ty Office will speak with us about biosafety as it relates to cell culture work. </font color> # Familiarize yourself with cell culture work by reading [[20.109(S07):Guidelines for working in the tissue culture facil9 KB (1,432 words) - 15:31, 15 June 2015
- ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of2 KB (360 words) - 15:31, 15 June 2015
- *Procedures for doing tissue culture work.1 KB (139 words) - 15:31, 15 June 2015
- ...faculty has set up four overnight LB + kan cultures of bacteria for you to work with. If your reactions were unsuccessful then you have been provided with ... enzymes you ll use. The following table may be helpful as you plan your work.11 KB (1,751 words) - 15:31, 15 June 2015
- ...ly complicated so im going to do it a slightly simpler way that will still work just as good.'''17 KB (2,960 words) - 15:31, 15 June 2015
- ...on of your work, in your lab notebook and on the wiki. By documenting your work according to the exercises done today, you will ...ave the same recognition sites? do they leave the same overhang? will they work in the same buffer? at the same temperature? These are some of the prelimin32 KB (5,419 words) - 15:31, 15 June 2015
- ...ple, they built a surrogate T7 that was a better template for experimental work, easier to manipulate and analyze, easier to characterize and understand. ...el should take approximately one hour to run. During that hour, you should work on parts two and three of today's protocol.<br>13 KB (2,004 words) - 15:31, 15 June 2015
- ...lls is extremely low (~10<sup>-7</sup>M). Calcium pumps and gated channels work hard to establish and keep this steep gradient. Inside the cell, calcium is ...range of such techniques. <b> If you remember only one detail from today's work, let it be the importance of choosing an appropriate tool for the measureme20 KB (3,237 words) - 15:45, 15 June 2015
- ...2.5 ul of Lipofectamine 2000 in 50 ul OptiMEM. You and your partner should work together to dilute enough "carrier" for 10 lipofections. Let the dilution s ...out the agent(s) you've chosen, thinking about the concentration(s) you'll work with and how you expect the chemicals to affect the fluorescence. <br>9 KB (1,436 words) - 15:31, 15 June 2015
- ... the fluorescent signal. You may also want to turn off the room lights and work with only the task light on.7 KB (1,103 words) - 15:31, 15 June 2015
- ...discuss the details of the upcoming presentation and you will have time to work on it with your partner. While these reactions are cycling, you and your partner should work on the research proposal that you will present to the class next time (see6 KB (1,087 words) - 15:31, 15 June 2015
- ... be used to sketch your experimental set up. It may be possible for you to work on the other parts of today's experiments while the yeast are incubating wi #*needed resources to complete the work10 KB (1,637 words) - 15:31, 15 June 2015
- ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of2 KB (372 words) - 15:54, 15 June 2015
- ==Sign your work==3 KB (494 words) - 15:54, 15 June 2015
- *Procedures for doing tissue culture work.903 B (119 words) - 15:54, 15 June 2015
- ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.6 KB (1,073 words) - 15:54, 15 June 2015
- ...in the "for next time" section of each lab day. <font color = red> You can work with your lab partner, friends and teaching assistants on these assignments <font color= red><b>Late work will be penalized 1/3 of a letter grade for each day late and will not be a3 KB (376 words) - 15:54, 15 June 2015
- ... your partner and to others in the lab if you are not up to speed when the work begins. ... have cut yourself (even if you consider it minor), if something broke and needs cleaning up, or if you are on fire.2 KB (375 words) - 15:54, 15 June 2015
- In the previous experimental module, your work has focused on DNA. In this experimental module, RNA gets the spotlight. Wh ... convert an effective inhibitor into a useless one. Conversely, siRNAs can work promiscuously and silence non-target genes, leading to effects on genes tha21 KB (3,532 words) - 15:54, 15 June 2015
- ... list the name of your partner on your report since she contributed to the work. ... trends, raw data (like a picture of a gel), or a model that explains your work.12 KB (1,850 words) - 15:54, 15 June 2015
- Our lab is beautifully equipped. We have a fume hood for work that generates hazardous vapors. We have a back room where dishes can be wa22 KB (3,670 words) - 15:54, 15 June 2015
- When you have a break from the work described below, be sure to examine the plaques you plated last time. Recor ...ch enzymes you are adding, and how many basepairs of DNA this modification needs. Next, use the plasmid map to help you plan at least two restriction digest16 KB (2,748 words) - 15:54, 15 June 2015
- ...ple, they built a surrogate T7 that was a better template for experimental work, easier to manipulate and analyze, easier to characterize and understand. N ...so read it carefully and then use some or none of the example for your own work.19 KB (3,059 words) - 16:00, 15 June 2015
- ..."electrophoresis," is something you're already familiar with from your DNA work. SDS-PAGE uses charge to separate proteins rather than DNA, but is not iden ...el should take approximately one hour to run. During that hour, you should work on part two of today's protocol.<br>11 KB (1,724 words) - 15:54, 15 June 2015
- ... to find or buy some for you. The ones you specify as part of your own lab work will be automatically purchased so don't fret if you don't see them here.549 B (99 words) - 15:54, 15 June 2015
- You are asked to write a formal lab report detailing your work in this module. Specifics for each section of this report are detailed belo *conclusions you can draw from your work, including any uncertainties3 KB (542 words) - 15:54, 15 June 2015
- ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.4 KB (733 words) - 15:54, 15 June 2015
- ...oose a section of the M13.1 scaffold in which you will do your refactoring work (you should choose what you want to refactor based on what seems interestin ... that helps p5 sequester the + strands more effectively, perhaps making p5 work more efficiently by reducing competition. <br>15 KB (2,532 words) - 15:54, 15 June 2015
- * needed resources to complete the work5 KB (796 words) - 15:54, 15 June 2015
- ...ientists who can tolerate a mix of p8 proteins on the phage coat for their work, there are phage-display variations that mix and match fusion and wild-type ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (e.g see reference [http://www.sciencemag.org/cgi/content/abstract/312/577510 KB (1,707 words) - 15:54, 15 June 2015
- ...color> there will be no lecture on 11.06.07 to give everyone extra time to work on their oral presentations. ...9(F07):_Agarose_gel_electrophoresis | talk page for that day in lab]. Nice work!!2 KB (334 words) - 15:54, 15 June 2015
- ...ments that haven't been done before and for letting everyone know how they work.4 KB (569 words) - 15:54, 15 June 2015
- ...equire a precise definition of life (think back to your Module 1 editorial work). In a top down approach, existing cells would be paired down to their mini You’ll work on two simple math problems for today: First, you’ll add a redesigned M1317 KB (2,797 words) - 15:54, 15 June 2015
- ...makes it science. There may be other ideas and explanations for how things work but when the preponderance of evidence supports an idea, it becomes the sci Scientific work generates evidence-based, internally consistent, and well-tested explanatio11 KB (1,890 words) - 15:55, 15 June 2015
- ...esearch proposal and its presentation. Next time you will have a chance to work out more details with your lab partner and then share your idea with anothe7 KB (1,172 words) - 15:55, 15 June 2015
- ... ug, 2 ug, 1 ug and 0.5 ug of each RNA sample that you prepared. Show your work. Save a copy of your answer since you'll need to know this volume to perfor9 KB (1,485 words) - 15:55, 15 June 2015
- ...polymerases, this enzyme can only add sequence to an existing chain and so needs a short “primer” to begin synthesis. To perform the cDNA synthesis, you ...NA only” pipet tips and solutions and wiping down your bench. You should work on a fresh piece of benchpaper and remember to wear gloves when working wit17 KB (2,883 words) - 15:55, 15 June 2015
- ...rovide complete and accurate information as well as for you to "sign" your work so we can attribute you properly. You should also write a summary paragraph13 KB (2,320 words) - 15:55, 15 June 2015
- ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of3 KB (514 words) - 19:05, 28 July 2015
- ... you choose, you might build your project after 109 is over, and then your work can be shown in one or more of the international exhibits that are describe ... The topic you choose, however, can be completely unrelated to any ongoing work in our department.12 KB (1,972 words) - 19:05, 28 July 2015
- ==Sign your work==3 KB (494 words) - 19:05, 28 July 2015
- ...equire a precise definition of life (think back to your Module 1 editorial work). In a top down approach, existing cells would be paired down to their mini You’ll work on two simple math problems for today: First, you’ll add a redesigned M1317 KB (2,797 words) - 19:05, 28 July 2015
- ...gulating protein stability, localization and modifications. Thus, from the work we've done so far this term, you may have the idea that gene expression is ...tocol for designing the reverse primer is just one method of many that can work. For example, taking the reverse complement can be done at any stage, makin29 KB (4,451 words) - 19:05, 28 July 2015
- ...lt projects should be tackled with the idea that they can, should and will work. Attitude alone, however, does not overcome pitfalls and there is much to b ...distinguish these possible explanations but it can tell you that something needs to be re-worked.19 KB (3,254 words) - 19:05, 28 July 2015
- ...ncorrosive, unmachinable, dinosaur killing metal that s relevant for our work in this module. Iridium, when oxidized to IrO2, has a d-orbital conductance ... in sterile water at least 100 ul of the phage concentration you decide to work with and be sure to add this data to your list of [[20.109(F07): ECD experi13 KB (2,275 words) - 19:05, 28 July 2015
- ...ng so, you may be switching SAGA-subunits and you will have to rethink the work you have planned so far. And if applicable, it will be worth noting the "ne ...review article] that was written to celebrate and highlight this important work on the structure of the SAGA complex. You and your partner will be randomly9 KB (1,467 words) - 19:05, 28 July 2015
- ... Science 1996] A full 10 years later there is still ongoing discussion and work on how to best annotate the genome. [http://www.ncbi.nlm.nih.gov/entrez/que ... different from DNA in its stability. Consequently it is more difficult to work with RNA in the lab. It is not the techniques themselves that are difficult21 KB (3,401 words) - 19:05, 28 July 2015
- ...polymerases, this enzyme can only add sequence to an existing chain and so needs a short “primer” to begin synthesis. To perform the cDNA synthesis, you ...NA only” pipet tips and solutions and wiping down your bench. You should work on a fresh piece of benchpaper and remember to wear gloves when working wit17 KB (2,875 words) - 19:05, 28 July 2015
- ... will be held this weekend and next week in case you have questions as you work to write up the Mod2 data. [http://openwetware.org/wiki/Natalie_Kuldell | N ...:TAPcollection Nature03.pdf| here (pdf of Nature 2003 paper describing the work)]] and [[Media:TAPcollection Supp Nat03.doc| here (.doc of Supp Info)]] is3 KB (565 words) - 19:05, 28 July 2015
- ...ientists who can tolerate a mix of p8 proteins on the phage coat for their work, there are phage-display variations that mix and match fusion and wild-type ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (e.g see reference [http://www.sciencemag.org/cgi/content/abstract/312/577516 KB (2,756 words) - 19:05, 28 July 2015
- ..."electrophoresis," is something you're already familiar with from your DNA work. SDS-PAGE uses charge to separate proteins rather than DNA, but is not iden ...el should take approximately one hour to run. During that hour, you should work on part two of today's protocol.<br>11 KB (1,723 words) - 19:05, 28 July 2015
- ...y Office] will speak with us about biosafety as it relates to cell culture work. </font color> ...d [http://www.ebi.ac.uk/clustalw/ CLUSTAL-W]. It's default settings should work fine for this alignment and you do not have to enter your email address unl14 KB (2,285 words) - 19:05, 28 July 2015
- ... establish good habits for documentation of your work. By documenting your work according to the exercises done today, you will ...re there are no spaces between or after the letters or your search won’t work. Change the color of the start codon to blue. Repeat the “Find…” proc23 KB (3,892 words) - 19:30, 28 July 2015
- Ligations generally work best when there is a 1:4 ratio of backbone to insert. You will ligate your10 KB (1,806 words) - 19:05, 28 July 2015
- *work best in the same buffer and at the same temperature.<br> ...faculty has set up four overnight LB + AMP cultures of bacteria for you to work with. Three of these cultures have colonies from the ligation reactions you10 KB (1,634 words) - 19:05, 28 July 2015
- ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.6 KB (1,075 words) - 19:05, 28 July 2015
- ...ientists who can tolerate a mix of p8 proteins on the phage coat for their work, there are phage-display variations that mix and match fusion and wild-type ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (e.g see reference [http://www.sciencemag.org/cgi/content/abstract/312/577510 KB (1,707 words) - 19:05, 28 July 2015
- ...esearch proposal and its presentation. Next time you will have a chance to work out more details with your lab partner and then share your idea with anothe7 KB (1,172 words) - 19:05, 28 July 2015
- ...rovide complete and accurate information as well as for you to "sign" your work so we can attribute you properly. You should also write a summary paragraph13 KB (2,320 words) - 19:05, 28 July 2015
- ...n is just how commonly '''large complexes of proteins carry out the cell's work.''' Indeed, it's reasonable to think that a careful cataloging of the cell' ..., "electrophoresis," is something we're already familiar with from our DNA work. SDS-PAGE uses charge to separate proteins rather than DNA, but is not iden25 KB (4,068 words) - 19:05, 28 July 2015
- * needed resources to complete the work5 KB (796 words) - 19:05, 28 July 2015
- ... establish good habits for documentation of your work. By documenting your work according to the exercises done today, you will ...re there are no spaces between or after the letters or your search won’t work. Change the color of the start codon to blue. Repeat the “Find…” proc22 KB (3,807 words) - 19:29, 28 July 2015
- *work best in the same buffer and at the same temperature.<br> ...faculty has set up four overnight LB + AMP cultures of bacteria for you to work with. Three of these cultures have colonies from the ligation reactions you11 KB (1,770 words) - 19:05, 28 July 2015
- ... your partner and to others in the lab if you are not up to speed when the work begins. ... have cut yourself (even if you consider it minor), if something broke and needs cleaning up, or if you are on fire.2 KB (376 words) - 19:05, 28 July 2015
- Ligations generally work best when there is a 1:4 ratio of backbone to insert. You will ligate your11 KB (1,817 words) - 19:05, 28 July 2015
- ...n this draft of your assignment in to the faculty''' but by beginning this work today, you'll have time to get feed back and have questions answered. You w10 KB (1,701 words) - 19:05, 28 July 2015
- *conclusions you can draw from your work, including any uncertainties *limitations of your work, e.g. what kinds of experiments/controls/samples would have been great to i8 KB (1,254 words) - 19:05, 28 July 2015
- ...in the "for next time" section of each lab day. <font color = red> You can work with your lab partner, friends and teaching assistants on these assignments <font color= red><b>Late work will be penalized 1/3 of a letter grade for each day late and will not be a3 KB (472 words) - 19:05, 28 July 2015
- ...ncorrosive, unmachinable, dinosaur killing metal that s relevant for our work in this module. Iridium, when oxidized to IrO2, has a d-orbital conductance ...u felt you did well during your presentation and 2 things you feel you can work on. This will be a 2 point FNT assignment that is assigned here to count as13 KB (2,079 words) - 19:05, 28 July 2015
- ...tocol for designing the reverse primer is just one method of many that can work. For example, taking the reverse complement can be done at any stage, makin ...this experimental module will be a formal research article describing your work. Some general requirements for this report are detailed [[20.109(F08):Guide30 KB (4,904 words) - 19:06, 28 July 2015
- ...lt projects should be tackled with the idea that they can, should and will work. Attitude alone, however, does not overcome pitfalls and there is much to b ...distinguish these possible explanations but it can tell you that something needs to be re-worked.18 KB (3,152 words) - 19:06, 28 July 2015
- ... you'll study for the rest of the module, and you will have to rethink the work you have planned so far. And if applicable, it will be worth noting the "ne ...MID:_15653319 ] that was written to celebrate and highlight this important work on the structure of the SAGA complex. You and your partner will be randomly9 KB (1,612 words) - 19:06, 28 July 2015
- ...tual manipulations of the Western are not time consuming, we'll also begin work on another portion of this experimental module, namely the microarray analy ...ou should photograph or scan your plates to include when you write up this work as a research article.20 KB (3,266 words) - 19:06, 28 July 2015
- ...polymerases, this enzyme can only add sequence to an existing chain and so needs a short “primer” to begin synthesis. To perform the cDNA synthesis, you ...NA only” pipet tips and solutions and wiping down your bench. You should work on a fresh piece of benchpaper and remember to wear gloves when working wit17 KB (2,864 words) - 19:06, 28 July 2015
- ...edit and complete the information associated with your terrific microarray work!7 KB (1,156 words) - 19:06, 28 July 2015
- The work for today will very much depend on the outcome of the meeting with the Graf * needed resources to complete the work (1 slide)10 KB (1,689 words) - 19:06, 28 July 2015
- ...figures, but if you do so, you must include the reference to the published work. Figures you use should not be downloaded from a web site. ... be used to detect homologous recombination in mammalian cells. The reader needs to know what the goal is: which plasmid did you set out to create? Part B o9 KB (1,494 words) - 19:06, 28 July 2015
- *Procedures for doing tissue culture work.883 B (115 words) - 19:06, 28 July 2015
- ... list the name of your partner on your report since she contributed to the work. ... trends, raw data (like a picture of a gel), or a model that explains your work.12 KB (1,922 words) - 19:06, 28 July 2015
- ...r this assignment, though you will receive feedback on the quality of your work.2 KB (319 words) - 19:06, 28 July 2015
- ...ments that haven't been done before and for letting everyone know how they work.1 KB (179 words) - 19:06, 28 July 2015
- ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.5 KB (761 words) - 19:06, 28 July 2015
- ... well-deserved break, I hope you're ready to head full force back into lab work and analysis. Today you will revisit MES cell culture, transfecting these c17 KB (2,783 words) - 19:34, 28 July 2015
- ...makes it science. There may be other ideas and explanations for how things work but when the preponderance of evidence supports an idea, it becomes the sci Scientific work generates evidence-based, internally consistent, and well-tested explanatio14 KB (2,345 words) - 19:34, 28 July 2015
- ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.5 KB (788 words) - 19:34, 28 July 2015
- ... list the name of your partner on your report since she contributed to the work. ... trends, raw data (like a picture of a gel), or a model that explains your work.13 KB (2,033 words) - 19:34, 28 July 2015
- ...en defined through traditional scientific studies of EnvZ, for example the work from Tom Silhavy's lab( [http://openwetware.org/wiki/PMID:_9721293 ] and [[ ...ld examine the bacterial photograph you set up last time and document your work and your ideas about the experiment.24 KB (3,897 words) - 19:39, 28 July 2015
- ...in, YPYDVPDYA, in sufficient for antibody recognition--an experiment we'll work on today. ...el should take approximately one hour to run. During that hour, you should work on part two of today's protocol.<br>17 KB (2,716 words) - 19:39, 28 July 2015
- ... gold and silver. You will have time while these reactions are going on to work on the research proposal idea you've got started with your lab partner. * needed resources to complete the work7 KB (1,146 words) - 19:39, 28 July 2015
- ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of3 KB (412 words) - 19:33, 28 July 2015
- ...iscussions during the development of this module, as well as for her prior work in developing a [http://openwetware.org/wiki/20.109:Module_2 related module3 KB (497 words) - 19:33, 28 July 2015
- ...let s return to thinking about the structure of cartilage for a bit. Our work in this module has focused on chondrocytes themselves (viability and morpho ... diluted antibody per well. Incubate for 90 min (at room temperature), and work on Parts 2 and 3 of today's protocol.10 KB (1,561 words) - 19:33, 28 July 2015
- *Procedures for doing tissue culture work.937 B (123 words) - 19:33, 28 July 2015
- ...s wasting a month of your time repeating experiments already proven not to work or reinventing the wheel. ...As you work, you can ask yourself why these stretches of the protein might work the way that they do, and how they might be changed.23 KB (3,877 words) - 19:33, 28 July 2015
- ...al of information in order to design mutagenized inverse pericams nice work! Today you will put your designs into practice. ...reaction, to ensure that all the reagents are working properly. You should work quickly but carefully, and keep your tube in a chilled container at all tim13 KB (2,155 words) - 19:33, 28 July 2015
- ...ke your teaching faculty very happy if you contribute to their preparatory work. Please label 2 large glass test tubes with your team color and sample name ...ft of the Materials and Methods section of your lab report, describing the work you have done up through today.13 KB (2,203 words) - 19:33, 28 July 2015
- ... your partner and to others in the lab if you are not up to speed when the work begins. ... have cut yourself (even if you consider it minor), if something broke and needs cleaning up, or if you are on fire.2 KB (386 words) - 19:33, 28 July 2015
- #Incubate on ice for 1 hour. (You might work on parts 2, 4, and 5 of today's protocols now, as well as assemble the mate ...motivate the audience to keep reading! How? Reveal the significance of the work through connections to both prior scientific accomplishments and future app21 KB (3,468 words) - 19:33, 28 July 2015
- ... has 15 μL of liquid in it. You might use the table below to guide your work. #Measure the absorbance of each sample at 595 nm. Work as quickly as you can, because the absorbance will continue to slowly chang14 KB (2,203 words) - 19:33, 28 July 2015
- As evidenced by Nagai s work, wild-type inverse pericam is not toxic to BL21(DE3) cells. Although it is ...our cells with IPTG, you will let the resultant protein factories do their work for 2-3 hours. During this time, you will evaluate the DNA from your two X#17 KB (2,822 words) - 19:33, 28 July 2015
- ... of the surrounding environment on cell phenotype. In particular, you will work with primary chondrocytes and/or mesenchymal stem cells in 3D gel culture. ...er time. We will also have some mesenchymal stem cells for a few groups to work with, and investigate conditions that promote chondrogenesis. Please see th5 KB (746 words) - 19:33, 28 July 2015
- Only 2-3 groups at a time will work in lab today. Last time you should have signed up to arrive at 1:05, 2:20 o #Now work your way from reservoirs #2 to #12 (highest calcium concentration), and fro7 KB (1,202 words) - 19:33, 28 July 2015
- ...got last time. Begin by analyzing the wild-type protein as a check on your work (your curve should resemble Nagai's Figure 3L), then move on to your mutant ... t be discouraged if your wild-type values do not exactly match Nagai s work, or if there is variation between Parts 1, 2, and 3. (As an example, for Pa14 KB (2,303 words) - 19:33, 28 July 2015
- ...polymerases, this enzyme can only add sequence to an existing chain and so needs a short primer to begin synthesis. To perform the cDNA synthesis, you ...se FREE pipet tips and solutions and wiping down your bench. You should work on a fresh piece of benchpaper and remember to wear gloves when working wit22 KB (3,652 words) - 19:33, 28 July 2015
- In the previous experimental module, your work focused on proteins, and to implement protein modifications, you manipulate ... convert an effective inhibitor into a useless one. Conversely, siRNAs can work promiscuously and silence non-target genes, leading to effects on genes tha22 KB (3,613 words) - 19:33, 28 July 2015
- ... number of cells you expect to have by Day 3 of this module. Show all your work, starting from the raw hemocytometer data. The following rules of thumb and ...f your own strengths and weaknesses can often help you improve your future work. After you give your presentation today, write a brief self-evaluation. Spe12 KB (1,999 words) - 19:33, 28 July 2015
- ...nce they will undergo partly different manipulations. You may also want to work on two separate worksheets, one for each collagen type.5 KB (904 words) - 19:33, 28 July 2015
- ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of3 KB (466 words) - 19:38, 28 July 2015
- ==Sign your work==3 KB (503 words) - 19:38, 28 July 2015
- *Procedures for doing tissue culture work.953 B (124 words) - 19:38, 28 July 2015
- ... your partner and to others in the lab if you are not up to speed when the work begins. ... have cut yourself (even if you consider it minor), if something broke and needs cleaning up, or if you are on fire.2 KB (429 words) - 19:38, 28 July 2015
- ...---have lagged behind in the design and construction of novel systems that work in reliable, scalable, intuitive ways. Biology simply does not always perfo3 KB (369 words) - 19:38, 28 July 2015
- ...9863670 CNN videotape of Angie Belcher teaching President Obama about this work]2 KB (278 words) - 19:38, 28 July 2015
- ...in the "for next time" section of each lab day. <font color = red> You can work with your lab partner, friends and teaching assistants on these assignments <font color= red><b>Late work will be penalized 1/3 of a letter grade for each day late and will not be a4 KB (514 words) - 19:38, 28 July 2015
- ... establish good habits for documentation of your work. By documenting your work according to the exercises done today, you will ...re there are no spaces between or after the letters or your search won’t work. Change the color of the start codon to blue. Repeat the “Find…” proc22 KB (3,783 words) - 19:44, 28 July 2015
- ...of Block Buffer to the plate. Wait another 60-90 minutes. In the meantime, work on your analysis (Part 3). ...ve us somewhat better separation than a 1% gel, but is not as difficult to work with as a 3% gel.16 KB (2,660 words) - 19:49, 28 July 2015
- Some of today's lab protocol will require that we work in small groups in the Belcher lab. We'll be using their balance to measure5 KB (921 words) - 19:38, 28 July 2015
- Ligations generally work best when there is a 1:4 ratio of backbone to insert. You will ligate your10 KB (1,743 words) - 19:38, 28 July 2015
- *work best in the same buffer and at the same temperature.<br> ...faculty has set up four overnight LB + AMP cultures of bacteria for you to work with. Three of these cultures have colonies from the ligation reactions you11 KB (1,830 words) - 19:38, 28 July 2015
- ...n this draft of your assignment in to the faculty''' but by beginning this work today, you'll have time to get feed back and have questions answered. You w10 KB (1,594 words) - 19:38, 28 July 2015
- ...figures, but if you do so, you must include the reference to the published work. Reference format requirements are described in [[20.109(F09):Guidelines fo ... be used to detect homologous recombination in mammalian cells. The reader needs to know what the goal is: which plasmid did you set out to create? Part B o7 KB (1,083 words) - 19:38, 28 July 2015
- ...-write option on this assignment, though you will receive feedback on your work.5 KB (747 words) - 19:38, 28 July 2015
- *conclusions you can draw from your work, including any uncertainties *limitations of your work, e.g. what kinds of experiments/controls/samples would have been great to i7 KB (1,101 words) - 19:38, 28 July 2015
- ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.5 KB (777 words) - 19:38, 28 July 2015
- ... list the name of your partner on your report since she contributed to the work. ... trends, raw data (like a picture of a gel), or a model that explains your work.13 KB (1,951 words) - 19:38, 28 July 2015
- * needed resources to complete the work5 KB (879 words) - 19:38, 28 July 2015
- ...): System engineering research article| research article]] describing this work is due in just over one week. This assignment is due by 11:00 a.m. on the d13 KB (2,115 words) - 19:38, 28 July 2015
- ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.6 KB (1,084 words) - 19:38, 28 July 2015
- ...ientists who can tolerate a mix of p8 proteins on the phage coat for their work, there are phage-display variations that mix and match fusion and wild-type ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (e.g see reference [http://www.sciencemag.org/cgi/content/abstract/312/577516 KB (2,666 words) - 19:38, 28 July 2015
- ...eral scheme for Two Component Signaling]] Fortunately evolution appears to work with motifs and many of these motifs behave in a reasonably modular fashion13 KB (2,163 words) - 19:38, 28 July 2015
- ... to make accessory pigments needed for the light-sensing fusion protein to work. These phycobillins are shown in the box at the left of the signaling pathw ...ortant to think about effective figures that summarize and anticipate your work. [[Image:BlackBox details.png|thumb|center|400px]]11 KB (1,859 words) - 19:39, 28 July 2015
- ...let s return to thinking about the structure of cartilage for a bit. Our work in this module has focused on chondrocytes themselves (viability and morpho ... diluted antibody per well. Incubate for 90 min (at room temperature), and work on Parts 2 and 3 of today's protocol.9 KB (1,466 words) - 19:49, 28 July 2015
- ...nce they will undergo partly different manipulations. You may also want to work on two separate worksheets, one for each collagen type.5 KB (919 words) - 19:49, 28 July 2015
- ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of3 KB (445 words) - 21:00, 28 July 2015
- ...---have lagged behind in the design and construction of novel systems that work in reliable, scalable, intuitive ways. Biology simply does not always perfo3 KB (443 words) - 21:00, 28 July 2015
- ...k on your report, ask questions of each other or the teaching faculty, and work on your oral presentation if that's coming up too. ...): System engineering research article| research article]] describing this work is due in just over one week. This assignment is due by 11:00 a.m. on the d10 KB (1,613 words) - 21:00, 28 July 2015
- ...9863670 CNN videotape of Angie Belcher teaching President Obama about this work]2 KB (313 words) - 21:00, 28 July 2015
- ...figures, but if you do so, you must include the reference to the published work. Reference format requirements are described in [[20.109(F10):Guidelines fo ... be used to detect homologous recombination in mammalian cells. The reader needs to know what the goal is: which plasmid did you set out to create? Part B o7 KB (1,131 words) - 21:00, 28 July 2015
- ==Sign your work==3 KB (461 words) - 21:00, 28 July 2015
- *Procedures for doing tissue culture work =Communicating your work=1 KB (184 words) - 21:00, 28 July 2015
- ...eral scheme for Two Component Signaling]] Fortunately evolution appears to work with motifs and many of these motifs behave in a reasonably modular fashion14 KB (2,180 words) - 21:00, 28 July 2015
- ... your partner and to others in the lab if you are not up to speed when the work begins. ... have cut yourself (even if you consider it minor), if something broke and needs cleaning up, or if you are on fire.2 KB (429 words) - 21:00, 28 July 2015
- ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.6 KB (1,084 words) - 21:00, 28 July 2015
- ...ist the name of your partner on your report since (s)he contributed to the work. Indicate that you are the primary author of the report by underlining your ... trends, raw data (like a picture of a gel), or a model that explains your work.18 KB (2,793 words) - 21:00, 28 July 2015
- ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.5 KB (815 words) - 21:00, 28 July 2015
- *conclusions you can draw from your work, including any uncertainties *limitations of your work, e.g. what kinds of experiments/controls/samples would have been great to i7 KB (1,095 words) - 21:00, 28 July 2015
- ... establish good habits for documentation of your work. By documenting your work according to the exercises done today, you will ...re there are no spaces between or after the letters or your search won t work. Change the color of the start codon to blue. Repeat the Find proc22 KB (3,783 words) - 21:00, 28 July 2015
- * needed resources to complete the work5 KB (880 words) - 21:00, 28 July 2015
- Ligations generally work best when there is a 1:4 ratio of backbone to insert. You will ligate your10 KB (1,744 words) - 21:00, 28 July 2015
- *work best in the same buffer and at the same temperature.<br> ...faculty has set up four overnight LB + AMP cultures of bacteria for you to work with. Three of these cultures have colonies from the ligation reactions you11 KB (1,830 words) - 21:00, 28 July 2015
- ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of3 KB (555 words) - 21:05, 28 July 2015
- *Procedures for doing tissue culture work. ==Communicating your Work==1 KB (172 words) - 21:05, 28 July 2015
- ... your partner and to others in the lab if you are not up to speed when the work begins. ... have cut yourself (even if you consider it minor), if something broke and needs cleaning up, or if you are on fire.2 KB (434 words) - 21:05, 28 July 2015
- You and your partner may work together on the lab practical. (Note: this will not be the case for future Before starting today's wet lab work, you may want to wipe down your pipettes and your benchtop with 70% ethanol10 KB (1,688 words) - 21:05, 28 July 2015
- ...ts low viscosity means that high weight percent solutions are tractable to work with, and that the solidified gel remains pliable rather than brittle. HR a ... the work you do in Module 1 in a formal lab report. To help you pace your work, as well as give you feedback early on, you will be required to draft small11 KB (1,852 words) - 21:05, 28 July 2015
- ... different from DNA in its stability. Consequently it is more difficult to work with RNA in the lab. It is not the techniques themselves that are difficult *Before you begin your experiment, clean your work area: (1) remove all clutter, (2) wipe down the benchtop with warm water an16 KB (2,632 words) - 21:05, 28 July 2015
- ...anscription reaction to make more of the RNA mixture. For this process to work, the 6-5 and 8-12 sequences must amplify at the same rate, a fact that has ...d compete with this interaction seems to work. In Prof. Niles s original work, he used a different RT-PCR kit that did not face this inhibition issue, wh8 KB (1,243 words) - 21:05, 28 July 2015
- ...e λ and Higher Organisms''. Despite having only 50,000 bp of DNA to work with, λ is able to exist in two distinct states — lytic or lys14 KB (2,340 words) - 21:05, 28 July 2015
- ...mework will be due. However, it is strongly suggested that you continue to work on your report, particularly the RT-PCR figure and experimental schematic. ...f your own strengths and weaknesses can often help you improve your future work. After you give your presentation today, write a brief self-evaluation. Spe2 KB (251 words) - 21:05, 28 July 2015
- ...f your own strengths and weaknesses can often help you improve your future work. After you give your presentation next time, write a brief self-evaluation.10 KB (1,596 words) - 21:05, 28 July 2015
- ...at 1:30 pm sharp in room 16-336. (Day 6 presentations will begin after lab work is finished, b/w 1:30 and 2 pm.)8 KB (1,210 words) - 21:05, 28 July 2015
- ...ronment might substantially affect their function. In this module, we will work on understanding and improving an edge detection system effected by bacteri ...pful discussions during early module development, as well as for her prior work in developing a [http://openwetware.org/wiki/20.109(F10):Module_2 related m3 KB (437 words) - 21:05, 28 July 2015
- ...While the gel runs, you will set up control digests to understand how they work and why we had to do this one for you in the interests of time. ...te the IPTG- and light-sensitive systems to each other. For the mapping to work well, you should treat all the different cell strains as consistently as po17 KB (2,967 words) - 21:05, 28 July 2015
- ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of4 KB (629 words) - 21:07, 28 July 2015
- Ligations generally work best when there is a 1:4 ratio of backbone to insert. You will ligate your10 KB (1,802 words) - 21:07, 28 July 2015
- ...in the "for next time" section of each lab day. <font color = red> You can work with your lab partner, friends and teaching assistants on these assignments <font color= red><b>Late work will be penalized 1/3 of a letter grade for each day late and will not be a4 KB (549 words) - 21:07, 28 July 2015
- *You will work alone on this lab practical, not with your lab partner.1 KB (179 words) - 21:08, 28 July 2015
- ... establish good habits for documentation of your work. By documenting your work according to the exercises done today, you will ...re there are no spaces between or after the letters or your search won t work. Change the color of the start codon to blue. Repeat the Find proc22 KB (3,809 words) - 21:08, 28 July 2015
- ...r this assignment, though you will receive feedback on the quality of your work. ...nt summary all along, through the "For Next Time" assignments or other lab work. This is your chance to show off your understanding of all you've done. As3 KB (528 words) - 21:08, 28 July 2015
- * needed resources to complete the work5 KB (879 words) - 21:08, 28 July 2015
- ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.10 KB (1,639 words) - 21:08, 28 July 2015
- ...t.edu/environment/ehs/about_ehs.html (EHS)] come to discuss tissue culture work with you. ...y| guidelines for mammalian cell culture.]] Next time, you will begin your work in the tissue culture facility. <br>12 KB (1,904 words) - 21:08, 28 July 2015
- *Procedures for doing tissue culture work =Communicating your work=2 KB (204 words) - 21:08, 28 July 2015
- ...o far and improve the content accordingly. If you have questions about the work, you have numerous [[20.109(F11):People| resources available to help you.]]10 KB (1,541 words) - 21:08, 28 July 2015
- ...faculty has set up four overnight LB + AMP cultures of bacteria for you to work with. Three of these cultures have colonies from the ligation reactions you ... enzymes you ll use. The following table may be helpful as you plan your work.13 KB (2,328 words) - 21:08, 28 July 2015
- *conclusions you can draw from your work, including any uncertainties *limitations of your work, e.g. what kinds of experiments/controls/samples would have been great to i7 KB (1,159 words) - 21:08, 28 July 2015
- ...---have lagged behind in the design and construction of novel systems that work in reliable, scalable, intuitive ways. Biology simply does not always perfo3 KB (445 words) - 21:08, 28 July 2015
- ...9 blog] and you, as students in 20.109, are being asked to reflect on your work in this lab class and then share your impressions. *Future directions you can imagine for the work2 KB (400 words) - 21:08, 28 July 2015
- ...eral scheme for Two Component Signaling]] Fortunately evolution appears to work with motifs and many of these motifs behave in a reasonably modular fashion ...gal assays you ran (best to include your experimental values and show your work for at least one calculation), then answer the following questions:14 KB (2,184 words) - 21:08, 28 July 2015
- ... to make accessory pigments needed for the light-sensing fusion protein to work. These phycobillins are shown in the box at the left of the signaling pathw ...ortant to think about effective figures that summarize and anticipate your work. [[Image:BlackBox details.png|thumb|center|400px]]21 KB (3,476 words) - 21:08, 28 July 2015
- The groups not visiting the Belcher lab should use the extra time to work on their research proposals. These will be presented in just one week!!5 KB (811 words) - 21:08, 28 July 2015
- ==Sign your work==3 KB (461 words) - 21:08, 28 July 2015
- ...en defined through traditional scientific studies of EnvZ, for example the work from Tom Silhavy's lab( [http://openwetware.org/wiki/PMID:_9721293 ] and [[ ...ld examine the bacterial photograph you set up last time and document your work and your ideas about the experiment.30 KB (4,934 words) - 21:08, 28 July 2015
- ...el should take approximately one hour to run. During that hour, you should work on part two of today's protocol.<br>16 KB (2,610 words) - 21:08, 28 July 2015
- ...k on your report, ask questions of each other or the teaching faculty, and work on your oral presentation if that's coming up too. ...ts of your choosing, to talk with other groups about their results, and to work on your research article.10 KB (1,644 words) - 21:08, 28 July 2015
- ...ientists who can tolerate a mix of p8 proteins on the phage coat for their work, there are phage-display variations that mix and match fusion and wild-type ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (e.g see reference [http://www.sciencemag.org/cgi/content/abstract/312/577518 KB (3,015 words) - 13:43, 29 July 2015
- ...9863670 CNN videotape of Angie Belcher teaching President Obama about this work]3 KB (362 words) - 13:43, 29 July 2015
- ... and then submit or present a proposal, as if you were seeking to fund the work. ...og this proposal development is up to you but be active in organizing your work or you'll find it much harder to pull all the content into a cohesive propo4 KB (640 words) - 13:43, 29 July 2015
- ...dea and then submit a written proposal, as if you were seeking to fund the work for the next '''3 years'''!2 KB (372 words) - 13:43, 29 July 2015
- * needed resources to complete the work2 KB (345 words) - 13:43, 29 July 2015
- ... inducing lysis), and also without contact using ultrasound[5], but little work has been done in using a sound input in an engineered system to initiate pr6 KB (833 words) - 13:43, 29 July 2015
- ===Sign your work===9 KB (1,512 words) - 13:48, 29 July 2015
- ...al of information in order to design mutagenized inverse pericams – nice work! Today you will put your designs into practice. ...reaction, to ensure that all the reagents are working properly. You should work quickly but carefully, and keep your tube in a chilled container at all tim13 KB (2,152 words) - 13:48, 29 July 2015
- ...of your labmates time.''' Reading the protocol in advance will help you work more quickly, and is strongly recommended. When observing your cells under fluorescence excitation, you should work with the room lights off for best results. A member of the teaching faculty15 KB (2,447 words) - 13:48, 29 July 2015
- ...work on Parts 3 and (optional) 5, label the tubes you will need in Part 4, work on your notebooks, start the FNT assignment (it's a little long for a weekd '''Please clearly show all your work and reasoning throughout this assignment. Parts A through C should be compl13 KB (2,181 words) - 13:48, 29 July 2015
- ...s wasting a month of your time repeating experiments already proven not to work or reinventing the wheel. ...As you work, you can ask yourself why these stretches of the protein might work the way that they do, and how they might be changed.26 KB (4,387 words) - 13:48, 29 July 2015
- #Incubate on ice for 1 hour. You can work on parts 2, 4, and 5 of today's protocols now, as well as assemble the mate ...ein_engineering_report | research article]] describing your protein design work. For this assignment, you will write start outlining and drafting the intro20 KB (3,344 words) - 13:48, 29 July 2015
- As evidenced by Nagai’s work, wild-type inverse pericam is not toxic to BL21(DE3) cells. Although it is ...our cells with IPTG, you will let the resultant protein factories do their work for 2-3 hours. During this time, you will evaluate the DNA from your two X#15 KB (2,584 words) - 13:48, 29 July 2015
- ... has 15 μL of liquid in it. You might use the table below to guide your work. #*The timing function on this centrifuge does not work! Bring your timer and manually turn the centrifuge off after 2 min. Start t13 KB (2,134 words) - 13:48, 29 July 2015
- ... of the surrounding environment on cell phenotype. In particular, you will work with primary chondrocytes and/or mesenchymal stem cells in 3D gel culture. ...er time. We will also have some mesenchymal stem cells for a few groups to work with in order to investigate conditions that promote chondrogenesis. Please18 KB (2,946 words) - 13:48, 29 July 2015
- ...did in previous reports. You can assume a reader highly familiar with your work, such as a graduate school advisor to whom you are providing an update. Summarize your findings and briefly suggest future work in about 2-3 pages, not counting figures. What we usually call Results and3 KB (415 words) - 13:48, 29 July 2015
- Before (second TC cohort) or after (first TC cohort) your wet-lab work today, take some time to discuss the five research results you wrote up for ...trix dissolution for better protein or proteoglycan recovery. Split up the work with your partner whatever way is most convenient. '''Remember to label you25 KB (4,196 words) - 13:48, 29 July 2015
- ... Buffer to the plate. Wait another 60-90 minutes. In the meantime, you can work on cell viability analysis (first listed on Day 4 Part 4). ...of RNA in your cDNA reaction, a 5-10x further dilution of your cDNA should work well.15 KB (2,467 words) - 13:48, 29 July 2015
- ...got last time. Begin by analyzing the wild-type protein as a check on your work (your curve should resemble Nagai's Figure 3L), and then move on to your mu ...t be discouraged if your wild-type values do not exactly match Nagai’s work, or if there is variation between Parts 1, 2, and 3.16 KB (2,718 words) - 13:48, 29 July 2015
- ...nce they will undergo partly different manipulations. You may also want to work on two separate worksheets, one for each collagen type.6 KB (932 words) - 13:48, 29 July 2015
- ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion. ...intrigue your reader. Keep in mind, you have little space to motivate your work and you need to catch the reader’s attention.8 KB (1,267 words) - 13:48, 29 July 2015
- ... elements, including organization, clarity, and proper attribution for the work. *Generally speaking, you should figure out how to work the lights, slide projector, curtains, etc. before you begin. In this class5 KB (895 words) - 13:48, 29 July 2015
- * needed resources to complete the work6 KB (923 words) - 13:48, 29 July 2015
- ...f your own strengths and weaknesses can often help you improve your future work. After you give your presentation, write a brief self-evaluation (200 words ... this revision that you think are generalizable and will translate to your work on the second report? Again, 200-250 words should suffice.5 KB (806 words) - 13:48, 29 July 2015
- ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed. ...motivate the audience to keep reading! How? Reveal the significance of the work through connections to both prior scientific accomplishments and interestin22 KB (3,317 words) - 13:48, 29 July 2015
- ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.7 KB (1,175 words) - 13:48, 29 July 2015
- ... you are in the second group, you may use the time that you are waiting to work on your FNT or the optional data analysis, but also be sure to prepare your ...trix dissolution for better protein or proteoglycan recovery. Split up the work with your partner whatever way is most convenient. '''Remember to label you24 KB (4,100 words) - 13:59, 29 July 2015
- ...let’s return to thinking about the structure of cartilage for a bit. Our work in this module so far has focused on on the cells themselves (viability and ... diluted antibody per well. Incubate for 90 min (at room temperature), and work on Parts 2 and 3 of today's protocol.16 KB (2,528 words) - 13:59, 29 July 2015
- ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.7 KB (1,207 words) - 13:59, 29 July 2015
- ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed. ... to keep reading! How do you do the latter? Reveal the significance of the work through connections to both prior scientific accomplishments and interestin22 KB (3,344 words) - 13:59, 29 July 2015
- ...did in previous reports. You can assume a reader highly familiar with your work, such as a graduate school advising committee to whom you are providing an Summarize your findings and briefly suggest future work in ~ 4 pages, give or take, not counting figures. What we usually call Resu3 KB (411 words) - 13:59, 29 July 2015
- * needed resources to complete the work ...n this proposal. For example, if you propose molecular and/or ''in vitro'' work, you would want to be able to say a few words about ''in vivo'' experiments14 KB (2,342 words) - 13:59, 29 July 2015
- #None: all work today is computer work. Get passing familiarity with the faculty-selected journal articles. #150 mM NaCl in autoclaved water--needs to be sterile filtered11 KB (1,791 words) - 13:59, 29 July 2015
- * Make a plan for preparing the high volume TC work we do. Lab also needs you to:16 KB (2,601 words) - 14:02, 29 July 2015
- ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of3 KB (481 words) - 14:02, 29 July 2015
- Ligations generally work best when there is a 1:4 '''molar''' ratio of backbone to insert. You will ...ngineering_summary | '''formal written summary''']]. To help you pace your work, as well as give you feedback early on, you will be required to draft small13 KB (2,248 words) - 14:02, 29 July 2015
- ...first, as it refreshes my memory and reminds me how exciting it was do the work. Next, I take this ‘writers high’ to the data section, which I find the6 KB (1,018 words) - 14:02, 29 July 2015
- Although your lab work will be done in pairs, <font color = CC0000>'''most assignments will be sub ...</font color> We strongly recommend that you plan ahead and space out your work when possible. For assignments that may be revised, be sure to read the lat9 KB (1,248 words) - 14:02, 29 July 2015
- ...in figures and supporting text – including context for understanding the work’s broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion. To aid in educating the audience, an In9 KB (1,410 words) - 14:02, 29 July 2015
- ... establish good habits for documentation of your work. By documenting your work according to the exercises done today, you will ...re there are no spaces between or after the letters or your search won’t work. Change the color of the start codon to blue. Repeat the “Find…” proc23 KB (3,882 words) - 14:02, 29 July 2015
- ... will confirm the presence of the PCR insert. Please clearly show all your work and reasoning, and be sure that your assignment explicitly includes * work best in the same buffer and at the same temperature11 KB (1,836 words) - 14:02, 29 July 2015
- ...faculty has set up four overnight LB + AMP cultures of bacteria for you to work with. Three of these cultures have colonies from the ligation reactions you ... enzymes you’ll use. The following table may be helpful as you plan your work. As an example, ''XbaI'' is 20,000 U/mL; therefore one reaction will requir20 KB (3,455 words) - 14:02, 29 July 2015
- While you were out, there was a significant amount of work done to complete your CometChip assay (Thank you, Isaak!). Below is a summa11 KB (1,850 words) - 14:02, 29 July 2015
- ...ur computer versus operating from the web based application (although both work). You can also download Evernote to your smart phone for ease of uploading29 KB (4,864 words) - 14:02, 29 July 2015
- ...---have lagged behind in the design and construction of novel systems that work in reliable, scalable, intuitive ways. Biology simply does not always perfo3 KB (506 words) - 14:02, 29 July 2015
- ...eral scheme for Two Component Signaling]] Fortunately evolution appears to work with motifs and many of these motifs behave in a reasonably modular fashion15 KB (2,383 words) - 14:02, 29 July 2015
- ... will confirm the presence of the PCR insert. Please clearly show all your work and reasoning in your lab notebook, and be sure that your plan explicitly i * work best in the same buffer and at the same temperature12 KB (2,060 words) - 14:02, 29 July 2015
- ... to make accessory pigments needed for the light-sensing fusion protein to work. These phycobilins are shown in the box at the left of the signaling pathwa21 KB (3,387 words) - 14:02, 29 July 2015
- ...en defined through traditional scientific studies of EnvZ, for example the work from Tom Silhavy's lab( [http://openwetware.org/wiki/PMID:_9721293 ] and [[ ...ld examine the bacterial photograph you set up last time and document your work and your ideas about the experiment.28 KB (4,560 words) - 14:02, 29 July 2015
- ...el should take approximately one hour to run. During that hour, you should work on part two of today's protocol.<br>15 KB (2,449 words) - 14:02, 29 July 2015
- ...k on your report, ask questions of each other or the teaching faculty, and work on your oral presentation if that's coming up too. ...ts of your choosing, to talk with other groups about their results, and to work on your research article.11 KB (1,712 words) - 14:02, 29 July 2015
- ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (e.g see reference [http://www.sciencemag.org/cgi/content/abstract/312/5775 Pre-lab work:16 KB (2,583 words) - 14:02, 29 July 2015
- * Make a plan for preparing the high volume TC work we do. Lab also needs you to:16 KB (2,511 words) - 14:02, 29 July 2015
- ...ith ethanol, and then with water. You will have time during these steps to work on the FNW, a first step toward developing a research proposal idea. Next t <font color = red>'''Today's lab has some safety hazards and you must work extremely carefully.'''</font color> Lab coats, gloves and goggles are a mu13 KB (2,165 words) - 14:02, 29 July 2015
- ===How does a TEM work?=== * needed resources to complete the work10 KB (1,709 words) - 14:02, 29 July 2015
- The groups not visiting the Belcher lab should use the extra time to work on their research proposals and get some peer feedback. Last time, you star11 KB (1,798 words) - 14:02, 29 July 2015
- ===Sign your work===9 KB (1,539 words) - 14:02, 29 July 2015
- Working with your partner, write a short report describing the Module 3 work according to the [[20.109%28F14%29:_Biomaterial_engineering_report | linked4 KB (620 words) - 14:02, 29 July 2015
- ... for this assignment, but you will receive substantive feedback about your work from a laboratory instructor. ...e, not the DNA repair assay phase. In other words, you should describe the work from M1D1-D5, NOT including tissue culture.3 KB (408 words) - 14:02, 29 July 2015
- ... will confirm the presence of the PCR insert. Please clearly show all your work and reasoning in your lab notebook, and be sure that your plan explicitly i * work best in the same buffer and at the same temperature12 KB (2,028 words) - 14:02, 29 July 2015
- ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it.2 KB (319 words) - 14:02, 29 July 2015
- ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed. ...motivate the audience to keep reading! How? Reveal the significance of the work through connections to both prior scientific accomplishments and interestin22 KB (3,322 words) - 14:02, 29 July 2015
- ... elements, including organization, clarity, and proper attribution for the work. *Generally speaking, you should figure out how to work the lights, slide projector, curtains, etc. before you begin. In this class5 KB (895 words) - 14:02, 29 July 2015
- ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.7 KB (1,163 words) - 14:02, 29 July 2015
- ...what the study contributed to the field. Maybe there's been some follow up work worth noting?3 KB (513 words) - 14:02, 29 July 2015
- *Procedures for doing tissue culture work =Communicating your work=1 KB (185 words) - 14:02, 29 July 2015
- ==Late Work== ...ade each day late and will not be accepted after a week.</font color> Only work turned in on time is guaranteed timely or extensive feedback.3 KB (479 words) - 14:02, 29 July 2015
- *front bench needs transparencies for printing. *Each pair needs ice bucket, electroporation cuvette, 2 MacLacMUG+Cam34+Amp25+Kan1015 KB (2,438 words) - 14:02, 29 July 2015
- ==Sign your work==3 KB (462 words) - 14:02, 29 July 2015
- ...nn.html CNN videotape of Angie Belcher teaching President Obama about this work]<br>4 KB (612 words) - 14:02, 29 July 2015
- * needed resources to complete the work ...n this proposal. For example, if you propose molecular and/or ''in vitro'' work, you would want to be able to say a few words about ''in vivo'' experiments14 KB (2,324 words) - 14:02, 29 July 2015
- ...summarize and interpret the class-wide findings and briefly suggest future work. What we usually call Results and Discussion should be combined. The Method |Contextualizing Results and Suggestions for Future Work4 KB (577 words) - 14:02, 29 July 2015
- *conclusions you can draw from your work, including any uncertainties *limitations of your work, e.g. what kinds of experiments/controls/samples would have been great to i7 KB (1,144 words) - 14:02, 29 July 2015
- ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed. *conclusions you can draw from your work, including any uncertainties8 KB (1,258 words) - 14:02, 29 July 2015
- ===Sign your work===9 KB (1,523 words) - 14:02, 29 July 2015
- ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of3 KB (482 words) - 17:15, 29 July 2015
- Although your lab work will be done in pairs, <font color = CC0000>'''most assignments will be sub ...</font color> We strongly recommend that you plan ahead and space out your work when possible. For assignments that may be revised, be sure to read the lat9 KB (1,366 words) - 17:15, 29 July 2015
- ...summarize and interpret the class-wide findings and briefly suggest future work. What we usually call Results and Discussion should be combined. The Method |Contextualizing Results and Suggestions for Future Work4 KB (570 words) - 17:15, 29 July 2015
- ...don't need to measure out this volume. Keep in mind that the washing steps work by dilution, so it is a balance between adding enough to create a sink for18 KB (2,985 words) - 17:15, 29 July 2015
- ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (e.g see reference [http://www.sciencemag.org/cgi/content/abstract/312/5775 Pre-lab work:16 KB (2,583 words) - 17:15, 29 July 2015
- ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (e.g see reference [http://www.sciencemag.org/cgi/content/abstract/312/5775 Pre-lab work:16 KB (2,577 words) - 17:15, 29 July 2015
- ===How does a TEM work?===8 KB (1,323 words) - 17:15, 29 July 2015
- ...crobiome data summary | abstract and data summary]]. To help you pace your work, as well as give you feedback early on, you will be required to draft small ...2-10x, with higher generally being better and at least 3-4x being known to work in our system.'''22 KB (3,588 words) - 17:15, 29 July 2015
- ...first, as it refreshes my memory and reminds me how exciting it was do the work. Next, I take this ‘writers high’ to the data section, which I find the6 KB (1,017 words) - 17:15, 29 July 2015
- ...l of the 16S rRNA sequencing data for their final report.''' Please try to work through this entire protocol today so that you are familiar with the steps #*''Why is it more convenient to work with the reverse complement when sequencing from the reverse direction?''24 KB (3,931 words) - 17:15, 29 July 2015
- ...ith ethanol, and then with water. You will have time during these steps to work on the FNW, a first step toward developing a research proposal idea. Next t <font color = red>'''Today's lab has some safety hazards and you must work extremely carefully.'''</font color> Lab coats, gloves and goggles are a mu14 KB (2,281 words) - 17:15, 29 July 2015
- ...nhibitors that had been used in human cells with success, but they did not work in our CHO cell system either. Therefore, we are calling on you -- the Spri ...to your fellow 109er about what they have done well and what needs further work. Complete this activity using the "golden rule" -- offer the type of feedba18 KB (2,885 words) - 17:15, 29 July 2015
- ...ke your teaching faculty very happy if you contribute to their preparatory work.16 KB (2,696 words) - 17:15, 29 July 2015
- #*As you work, tip the plate down a little to pool the media at the bottom of each well.14 KB (2,396 words) - 17:15, 29 July 2015
- ... many colonies, today we will use a commercially available kit so that the work can go more quickly. The principle is the same as that of our "quick and di In another week you will finally get to see the results of all your hard work!16 KB (2,688 words) - 17:15, 29 July 2015
- ...se primer bind to ''different'' strands of DNA. You may find it easiest to work with only the coding strand sequence at first, and then take the complement13 KB (2,073 words) - 17:15, 29 July 2015
- ...your perspective – it turns out in scientific research that the hard work is just beginning once the data is quantified! Interpreting the data and dr The one piece of wet lab work that you will do next time is complete your NHEJ inhibitor validation assay15 KB (2,568 words) - 17:15, 29 July 2015
- ==Late Work== ...ade each day late and will not be accepted after a week.</font color> Only work turned in on time is guaranteed timely or extensive feedback.3 KB (478 words) - 17:15, 29 July 2015
- ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (e.g see reference [http://www.sciencemag.org/cgi/content/abstract/312/5775 Pre-lab work:16 KB (2,579 words) - 17:15, 29 July 2015
- ... elements, including organization, clarity, and proper attribution for the work. *Generally speaking, you should figure out how to work the lights, slide projector, curtains, etc. before you begin. In this class5 KB (894 words) - 17:15, 29 July 2015
- ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.7 KB (1,162 words) - 17:15, 29 July 2015
- ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed. ...motivate the audience to keep reading! How? Reveal the significance of the work through connections to both prior scientific accomplishments and interestin21 KB (3,275 words) - 17:15, 29 July 2015
- ...ross which you will compare repair: cell type and damage topology. We will work exclusively with Chinese hamster ovary (CHO) cells; wild-type cells are cal ...e. Be sure to keep the conical tube containing your cells capped while you work at the microscope.18 KB (3,046 words) - 17:15, 29 July 2015
- *Procedures for doing tissue culture work =Communicating your work=1 KB (184 words) - 17:15, 29 July 2015
- ...ur computer versus operating from the web based application (although both work). You can also download Evernote to your smart phone for ease of uploading25 KB (4,176 words) - 17:15, 29 July 2015
- ...s transmission) is the flu virus. For your safety, all the samples we will work with today have been screened to exclude those carrying human–pathogenic Returning to today's specific work, each of you will extract a DNA pool from a single bird cloacal sample usin15 KB (2,507 words) - 17:15, 29 July 2015
- ...igures and supporting text – including context for understanding the work’s broader implications. ...; of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.14 KB (2,270 words) - 17:15, 29 July 2015
- ===Sign your work===9 KB (1,538 words) - 17:15, 29 July 2015
- #*''Why is it more convenient to work with the reverse complement when sequencing from the reverse direction?''15 KB (2,410 words) - 17:15, 29 July 2015
- * needed resources to complete the work ...n this proposal. For example, if you propose molecular and/or ''in vitro'' work, you would want to be able to say a few words about ''in vivo'' experiments14 KB (2,323 words) - 17:15, 29 July 2015
- The groups not visiting the Belcher lab should use the extra time to work on their research proposals and get some peer feedback. Last time, you star #*needed resources to complete the work13 KB (2,017 words) - 17:15, 29 July 2015
- ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it.2 KB (319 words) - 17:15, 29 July 2015
- ...T. I learned basic cognition tests and how to edit fMRI scans. I currently work at the Jasanoff Lab. I'm working on a PAG/MFB study in rats. I've learned h3 KB (433 words) - 17:15, 29 July 2015
- Working with your partner, write a short report describing the Module 3 work according to the [[20.109%28S15%29:_Biomaterial_engineering_report | linked4 KB (617 words) - 17:15, 29 July 2015
- ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of4 KB (660 words) - 17:30, 29 July 2015
- ... done in pairs, <font color = CC0000>'''you must submit individual written work (for both daily homeworks and major assignments) and give individual journa ...</font color> We strongly recommend that you plan ahead and space out your work when possible.8 KB (1,121 words) - 17:30, 29 July 2015
- ...mdash; especially your partner — if you are not up to speed when the work begins. #Do not work with chemicals until you are sure of their safe handling. Be aware of their2 KB (431 words) - 17:30, 29 July 2015
- ===Sign your work===7 KB (1,136 words) - 17:30, 29 July 2015
- ...f your own strengths and weaknesses can often help you improve your future work. After you give your presentation, write a brief self-evaluation (200 words11 KB (1,870 words) - 17:30, 29 July 2015
- You and your partner may work together on the lab practical. (Note: this will not be the case for future Before starting today's wet lab work, you may want to wipe down your pipettes and your benchtop with 70% ethanol10 KB (1,682 words) - 17:30, 29 July 2015
- ...ts low viscosity means that high weight percent solutions are tractable to work with, and that the solidified gel remains pliable rather than brittle. HR a ... the work you do in Module 1 in a formal lab report. To help you pace your work, as well as give you feedback early on, you will be required to draft small11 KB (1,844 words) - 17:30, 29 July 2015
- ... different from DNA in its stability. Consequently it is more difficult to work with RNA in the lab. It is not the techniques themselves that are difficult *Before you begin your experiment, clean your work area: (1) remove all clutter, (2) wipe down the benchtop with warm water an16 KB (2,671 words) - 17:30, 29 July 2015
- ...anscription reaction to make more of the RNA mixture. For this process to work, the 6-5 and 8-12 sequences must amplify at the same rate, a fact that has ...d compete with this interaction seems to work. In Prof. Niles’s original work, he used a different RT-PCR kit that did not face this inhibition issue, wh8 KB (1,365 words) - 17:30, 29 July 2015
- ...f your own strengths and weaknesses can often help you improve your future work. After you give your presentation, write a brief self-evaluation (200 words2 KB (252 words) - 17:30, 29 July 2015
- ...at 1:30 pm sharp in room 16-336. (Day 6 presentations will begin after lab work is finished, b/w 1:30 and 2 pm.)7 KB (1,085 words) - 17:30, 29 July 2015
- ...of your labmates time.''' Reading the protocol in advance will help you work more quickly, and is strongly recommended. ...performing the viability assay, and/or during incubation steps, you should work on Part 3 of today's protocol.17 KB (2,877 words) - 17:30, 29 July 2015
- ...iscussions during the development of this module, as well as for her prior work in developing a [http://openwetware.org/wiki/20.109:Module_2 related module4 KB (535 words) - 17:30, 29 July 2015
- ...s wasting a month of your time repeating experiments already proven not to work or reinventing the wheel. ...As you work, you can ask yourself why these stretches of the protein might work the way that they do, and how they might be changed.26 KB (4,263 words) - 17:30, 29 July 2015
- ...al of information in order to design mutagenized inverse pericams – nice work! Today you will put your designs into practice. ...reaction, to ensure that all the reagents are working properly. You should work quickly but carefully, and keep your tube in a chilled container at all tim13 KB (2,109 words) - 17:30, 29 July 2015
- ...work on Parts 3 and (optional) 5, label the tubes you will need in Part 4, work on your notebooks, start the FNT assignment, etc. '''Be sure to pre-chill y ...ke your teaching faculty very happy if you contribute to their preparatory work. Please label 2 large glass test tubes with your team color and sample name17 KB (2,889 words) - 17:30, 29 July 2015
- #Incubate on ice for 1 hour. You can work on parts 2, 4, and 5 of today's protocols now, as well as assemble the mate ===Microbial work===19 KB (3,278 words) - 17:30, 29 July 2015
- As evidenced by Nagai’s work, wild-type inverse pericam is not toxic to BL21(DE3) cells. Although it is ...our cells with IPTG, you will let the resultant protein factories do their work for 2-3 hours. During this time, you will evaluate the DNA from your two X#17 KB (2,911 words) - 17:30, 29 July 2015
- ... has 15 μL of liquid in it. You might use the table below to guide your work. #*The timing function on this centrifuge does not work! Bring your timer and manually turn the centrifuge off after 2 min. Start t13 KB (2,041 words) - 17:30, 29 July 2015
- ...people can compare their own mutants to those of other teams doing similar work. #Now work your way from reservoirs #2 to #12 (highest calcium concentration), and fro23 KB (3,809 words) - 17:30, 29 July 2015
- ...let s return to thinking about the structure of cartilage for a bit. Our work in this module so far has focused on on the cells themselves (viability and ... diluted antibody per well. Incubate for 90 min (at room temperature), and work on Parts 2 and 3 of today's protocol.17 KB (2,752 words) - 17:30, 29 July 2015
- ... elements, including organization, clarity, and proper attribution for the work. *Generally, figure out how to work the lights, slide projector, curtains, etc. before you begin. In this class5 KB (893 words) - 17:30, 29 July 2015
- ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.5 KB (796 words) - 17:30, 29 July 2015
- ...f 20.109, we do not expect you to know the details of how these algorithms work.12 KB (1,846 words) - 17:30, 29 July 2015
- ...o you should decide who will seed at 1:10 and who will seed at 1:2. As you work, try to put into practice what you learned about sterile technique in the d ... of the surrounding environment on cell phenotype. In particular, you will work with primary chondrocytes and/or mesenchymal stem cells in 3D gel culture.16 KB (2,574 words) - 17:30, 29 July 2015
- * needed resources to complete the work5 KB (905 words) - 17:30, 29 July 2015
- ...eir knee joints is more similar to that of humans. In this module, we will work with an ''in vitro'' culture model of cartilage-forming cells. Half the class at a time will work in the tissue culture room today. Today will be physically and mentally lab10 KB (1,708 words) - 17:30, 29 July 2015
- ...trix dissolution for better protein or proteoglycan recovery. Split up the work with your partner whatever way is most convenient. '''Remember to label you ...did when washing your freshly synthesized beads. A 10 mL pipet size should work well for most beads, while for very delicate beads, you should use a 2 mL s19 KB (3,261 words) - 17:30, 29 July 2015
- ... Buffer to the plate. Wait another 60-90 minutes. In the meantime, you can work on cell viability analysis (first listed on Day 4 Part 4). ...of RNA in your cDNA reaction, a 5-10x further dilution of your cDNA should work well.13 KB (2,192 words) - 17:30, 29 July 2015
- ...nce they will undergo partly different manipulations. You may also want to work on two separate worksheets, one for each collagen type.6 KB (929 words) - 17:30, 29 July 2015
- ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed. ...motivate the audience to keep reading! How? Reveal the significance of the work through connections to both prior scientific accomplishments and interestin21 KB (3,191 words) - 17:30, 29 July 2015
- ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.7 KB (1,163 words) - 17:30, 29 July 2015
- ...f your own strengths and weaknesses can often help you improve your future work. After you give your presentation, write a brief self-evaluation (200 words ... this revision that you think are generalizable and will translate to your work on the second report? Again, 200-250 words should suffice.5 KB (799 words) - 17:30, 29 July 2015
- ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of ...is semester and get to know each other well. Everyone in the 20.109 family needs the support of their peers and instructors. If you find yourself in a situa7 KB (1,152 words) - 17:33, 29 July 2015
- * needed resources to complete the work ...n this proposal. For example, if you propose molecular and/or ''in vitro'' work, you would want to be able to say a few words about ''in vivo'' experiments14 KB (2,304 words) - 17:33, 29 July 2015
- Although your lab work will be done in pairs, <font color = CC0000>'''most assignments will be sub ...</font color> We strongly recommend that you plan ahead and space out your work when possible. For assignments that may be revised, be sure to read the lat8 KB (1,188 words) - 17:33, 29 July 2015
- ... establish good habits for documentation of your work. By documenting your work according to the exercises done today, you will ...re there are no spaces between or after the letters or your search won’t work. Change the color of the start codon to blue. Repeat the “Find…” proc21 KB (3,687 words) - 17:33, 29 July 2015
- ...ut you don't need to measure this out. Keep in mind that the washing steps work by dilution, so it is a balance between adding enough to create a sink for Scanning your Western blot is only the first step! You just did ''a lot'' of work to obtain what appears to be observational data. However, we can use densit17 KB (2,799 words) - 17:33, 29 July 2015
- ... fragment sizes expected for each double digest below. '''Please show your work.''' ...ngineering_methods | '''formal methods section''']]. To help you pace your work, as well as give you feedback early on, you will be required to draft and/o11 KB (1,881 words) - 17:33, 29 July 2015
- ...th and 2. The authors of the original model are '''always''' cited in your work.26 KB (4,292 words) - 17:33, 29 July 2015
- ...y| guidelines for working in the tissue culture facility]], where you will work next time. You can also peek ahead at steps 1 and 2 in Part 4 and start pre ... will confirm the presence of the PCR insert. Please clearly show all your work and reasoning, and be sure that your assignment explicitly includes11 KB (1,914 words) - 17:33, 29 July 2015
- Ligations generally work best when there is a 1:4 '''molar''' ratio of backbone to insert. You will ...ngineering_summary | '''formal written summary''']]. To help you pace your work, as well as give you feedback early on, you will be required to draft small13 KB (2,222 words) - 17:33, 29 July 2015
- ...faculty has set up four overnight LB + AMP cultures of bacteria for you to work with. Three of these cultures have colonies from the ligation reactions you ... enzymes you’ll use. The following table may be helpful as you plan your work. As an example, ''XbaI'' is 20,000 U/mL; therefore one reaction will requir17 KB (2,866 words) - 17:33, 29 July 2015
- ...nn.html CNN videotape of Angie Belcher teaching President Obama about this work]<br>4 KB (557 words) - 17:33, 29 July 2015
- ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.7 KB (1,163 words) - 17:33, 29 July 2015
- ===Sign your work===9 KB (1,523 words) - 17:33, 29 July 2015
- ...mdash; especially your partner — if you are not up to speed when the work begins. #Do not work with chemicals until you are sure of their safe handling. Be aware of their3 KB (500 words) - 17:33, 29 July 2015
- ... but also lets others independently assess the merits of both the original work and the conclusions you have drawn from it.2 KB (319 words) - 17:33, 29 July 2015
- ...ill employ a 96-well multichannel pipette to significantly cut down on our work -- giving us a different method to minimize our pipetting steps.9 KB (1,414 words) - 17:33, 29 July 2015
- The groups not visiting the Belcher lab should use the extra time to work on their research proposals and get some peer feedback. Last time, you star * needed resources to complete the work13 KB (2,096 words) - 17:33, 29 July 2015
- ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (e.g see reference [http://www.sciencemag.org/cgi/content/abstract/312/5775 Pre-lab work:18 KB (2,861 words) - 17:33, 29 July 2015
- ... elements, including organization, clarity, and proper attribution for the work. *Generally speaking, you should figure out how to work the lights, slide projector, curtains, etc. before you begin. In this class5 KB (895 words) - 17:33, 29 July 2015
- ...rigue your reader. '''Keep in mind, you have little space to motivate your work and you need to catch the reader’s attention.''' ...stance might be overcome in the clinic? Are human studies relevant to this work? Studies in other organisms (cell lines, mice, etc)?10 KB (1,586 words) - 17:33, 29 July 2015
- ...ith ethanol, and then with water. You will have time during these steps to work on the FNT, a first step toward developing a research proposal idea. Next t Today's lab has some safety hazards and you must work extremely carefully. Lab coats, gloves and goggles are a must when you're a15 KB (2,422 words) - 17:33, 29 July 2015
- ===How does a TEM work?===8 KB (1,330 words) - 17:33, 29 July 2015
- ...summarize and interpret the class-wide findings and briefly suggest future work. What we usually call Results and Discussion should be combined. The Method |Contextualizing Results and Suggestions for Future Work4 KB (650 words) - 17:33, 29 July 2015
- ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed. ...motivate the audience to keep reading! How? Reveal the significance of the work through connections to both prior scientific accomplishments and interestin22 KB (3,319 words) - 17:33, 29 July 2015
- Working with your partner, write a short report describing the Module 3 work according to the [[20.109%28F13%29:_Biomaterial_engineering_report | linked4 KB (638 words) - 17:33, 29 July 2015
- ... for this assignment, but you will receive substantive feedback about your work from a laboratory instructor. ...e, not the DNA repair assay phase. In other words, you should describe the work from M1D1-D5.3 KB (433 words) - 17:33, 29 July 2015
- ...in figures and supporting text – including context for understanding the work’s broader implications. ... of technical information, and consequently should strive to present your work in a logical, step-by-step fashion.10 KB (1,548 words) - 17:33, 29 July 2015
- ... different from DNA in its stability. Consequently it is more difficult to work with RNA in the lab. It is not the techniques themselves that are difficult *Before you begin your experiment, clean and prepare your work area: (1) remove all clutter, (2) wipe down the benchtop with warm water an18 KB (2,944 words) - 17:33, 29 July 2015
- ...ut you don't need to measure this out. Keep in mind that the washing steps work by dilution, so it is a balance between adding enough to create a sink for Scanning your Western blot is only the first step! You just did ''a lot'' of work to obtain what appears to be observational data. However, we can use densit16 KB (2,673 words) - 17:33, 29 July 2015
- ...first, as it refreshes my memory and reminds me how exciting it was do the work. Next, I take this ‘writers high’ to the data section, which I find the6 KB (916 words) - 17:33, 29 July 2015
- *(1) How do MM-111 and Lapatinib work? (You may need to read ahead to the Ligand-receptor interactions section to ... be changed if the assumptions experimentally verified in S9 & S10 did not work out?23 KB (3,661 words) - 17:42, 29 July 2015
- ...verify your situation, and then discuss with you how to address the missed work. Students will not be excused from coursework without verification from St ...for accommodations, please contact me early in the semester so that we can work together to get your accommodation logistics in place.1 KB (217 words) - 17:33, 29 July 2015
- ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of3 KB (459 words) - 19:48, 28 July 2015
- ... has 15 μL of liquid in it. You might use the table below to guide your work. #Measure the absorbance of each sample at 595 nm. Work as quickly as you can, because the absorbance will continue to slowly chang14 KB (2,165 words) - 19:48, 28 July 2015
- ...mework will be due. However, it is strongly suggested that you continue to work on your report, particularly the RT-PCR figure and experimental schematic. **You should work on Part III of the computational assignment, and hand in a picture of the f2 KB (267 words) - 19:49, 28 July 2015
- *Procedures for doing tissue culture work. ==Communicating your Work==1 KB (162 words) - 19:49, 28 July 2015
- ... your partner and to others in the lab if you are not up to speed when the work begins. ... have cut yourself (even if you consider it minor), if something broke and needs cleaning up, or if you are on fire.2 KB (386 words) - 19:49, 28 July 2015
- ...f your own strengths and weaknesses can often help you improve your future work. After you give your presentation next time, write a brief self-evaluation.9 KB (1,560 words) - 19:49, 28 July 2015
- ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.7 KB (1,148 words) - 19:49, 28 July 2015
- ==Sign your work==3 KB (494 words) - 19:49, 28 July 2015
- ...iscussions during the development of this module, as well as for her prior work in developing a [http://openwetware.org/wiki/20.109:Module_2 related module3 KB (497 words) - 19:49, 28 July 2015
- You and your partner may work together on the lab practical. (Note: this will not be the case for future Before starting today's wet lab work, you may want to wipe down your pipettes and your benchtop with 70% ethanol10 KB (1,656 words) - 19:49, 28 July 2015
- ...ts low viscosity means that high weight percent solutions are tractable to work with, and that the solidified gel remains pliable rather than brittle. HR a ... the work you do in Module 1 in a formal lab report. To help you pace your work, as well as give you feedback early on, you will be required to draft small11 KB (1,764 words) - 19:49, 28 July 2015
- ... different from DNA in its stability. Consequently it is more difficult to work with RNA in the lab. It is not the techniques themselves that are difficult *Before you begin your experiment clean your work area, removing all clutter. Wipe down the benchtop with warm water then “15 KB (2,450 words) - 19:49, 28 July 2015
- ...anscription reaction to make more of the RNA aptamer. For this process to work, the 6-5 and 8-12 aptamers must amplify at the same rate, a fact that has b ...o compete with this interaction seems to work. If Prof. Niles’s original work, he used a different RT-PCR kit that did not face this inhibition issue, wh7 KB (1,218 words) - 19:49, 28 July 2015
