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| | R/F Oct 17/18 | | | R/F Oct 17/18 |
| | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa18 M2D3 figures.pptx| Article Figures]]<br>[[Media:Fa19 M2D3 paper discussion slides.pdf| Lecture slides]] | | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa18 M2D3 figures.pptx| Article Figures]]<br>[[Media:Fa19 M2D3 paper discussion slides.pdf| Lecture slides]] |
− | | [[20.109(F19):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br> | + | | [[20.109(F19):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br> [[Media:Fa19 M2D3 bcm marked.pdf | WF Prelab]] |
| | [[20.109(F19):Homework#Due_M2D3|Homework due]]<br>[[20.109(F19): Mini-presentation| Mini-presentation due]] Sat, Oct 19 at 10 pm | | | [[20.109(F19):Homework#Due_M2D3|Homework due]]<br>[[20.109(F19): Mini-presentation| Mini-presentation due]] Sat, Oct 19 at 10 pm |
| |-- | | |-- |
Revision as of 20:29, 18 October 2019
20.109(F19): Laboratory Fundamentals of Biological Engineering
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm (16-220)
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm (56-322)
MODULE
|
DAY
|
DATE
|
LECTURER
|
LABORATORY EXPERIMENTS
|
ASSIGNMENTS
|
|
|
R/F Sept 5/6
|
NLL Orientation slides
|
Orientation and laboratory tour Prelab T/R Prelab W/F
|
|
|
1
|
1
|
T/W Sept 10/11
|
BE
|
Learn optimal methods for culturing mammalian cells and initiate repair foci analysis experiment Prelab T/R Prelab W/F
|
Laboratory orientation quiz Homework due
|
1
|
2
|
R/F Sept 12/13
|
BE
|
Perform cell exposures for repair foci experiment and perform high-throughput genome damage pilot Prelab T/R Prelab W/F
|
Homework due
|
1
|
3
|
T/W Sept 17/18
|
BE
|
Perform immunofluorescence assay for repair foci and optimize high-throughput genome damage assay Prelab T/R Prelab W/F
|
Homework due
|
|
|
R/F Sept 19/20
|
Comm Lab
|
Lecture, but no laboratory Career fair student holiday
|
|
1
|
4
|
T/W Sept 24/25
|
BE
|
Perform immunofluorescence imaging for repair foci and expose cells for high-throughput genome damage assay Prelab T/R Prelab W/F
|
Laboratory quiz Homework due
|
1
|
5
|
R/F Sept 26/27
|
BE
|
Quantify repair foci via network algorithm and complete high-throughput genome damage assay Dushan's Talk TR Prelab WF Prelab
|
Homework due
|
1
|
6
|
T/W Oct 1/2
|
BE
|
Perform quantitative image analysis for the high-throughput genome damage assay TR Prelab WF Prelab
|
Homework due
|
1
|
7
|
R/F Oct 3/4
|
BE
|
Complete data analysis and apply statistical methods TR Prelab WF Prelab
|
Laboratory quiz Homework due
|
2
|
1
|
T/W Oct 8/9
|
NLL Lecture slides
|
Complete in silico cloning of pdCas9 TR Prelab WF Prelab
|
Homework due
|
2
|
2
|
R/F Oct 10/11
|
NLL Lecture slides
|
Design gRNA sequence for CRISPRi TR Prelab WF Prelab
|
Homework due
|
|
|
T/W Oct 15/16
|
|
Columbus day holiday
|
Data Summary draft due Mon, Oct 14 at 10 pm Blog post due Fri, Oct 18 at 10 pm
|
|
2
|
3
|
R/F Oct 17/18
|
NLL Article Figures Lecture slides
|
Generate gRNA plasmid WF Prelab
|
Homework due Mini-presentation due Sat, Oct 19 at 10 pm
|
2
|
4
|
T/W Oct 22/23
|
NLL
|
Journal club I
|
|
2
|
5
|
R/F Oct 24/25
|
NLL
|
Confirm gRNA sequence in pgRNA
|
Laboratory quiz Homework due Data Summary revision due Sat, Oct 26 at 10 pm
|
2
|
6
|
T/W Oct 29/30
|
NLL
|
Journal club II
|
|
2
|
7
|
R/F Oct/Nov 31/1
|
NLL
|
Induce CRISPRi system
|
Homework due Blog post due Fri, Nov 1 at 10 pm
|
2
|
8
|
T/W Nov 5/6
|
NLL
|
Measure fermentation products
|
Laboratory quiz Homework due
|
|
3
|
1
|
R/F Nov 7/8
|
AK
|
Prepare for cellular thermal shift assay
|
Homework due
|
|
|
T/W Nov 12/13
|
|
Veterans' day holiday
|
Research article due Mon, Nov 11 at 10 pm Blog post due Tue, Nov 12 at 10 pm
|
3
|
2
|
R/F Nov 14/15
|
AK
|
Incubate with ligand and apply heat treatment for protein denaturation
|
Homework due
|
3
|
3
|
T/W Nov 19/20
|
AK
|
Begin Western blot analysis
|
Laboratory quiz Homework due
|
3
|
4
|
R/F Nov 21/22
|
AK
|
Complete antibody staining for Western blot analysis
|
Homework due
|
|
|
T/W Nov 26/27
|
|
Lecture: research proposal pitch, but no laboratory
|
|
|
|
R/F Nov 28/29
|
|
Thanksgiving holiday
|
|
3
|
5
|
T/W Dec 3/4
|
AK
|
Data analysis and assignment preparation
|
Laboratory quiz Homework due
|
|
|
R/F Dec 5/6
|
|
Research proposal presentations
|
Blog post due Fri, Dec 6 at 10 pm
|
|
|
|
T Dec 10
|
|
Feedback and celebratory lunch
|
Mini-report due Mon, Dec 9 at 10 pm
|