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Revision as of 20:40, 6 May 2019
Contents
Module 1: Assessing ligand binding
M1D3: SMM analysis
Files for SMM analysis
GAL files linked here.
Scan images and set numbers assigned to each team:
Team | Slide #1 | Slide #2 |
T/R | ||
Red | 14399983, set 1 | 14400077, set 4 |
Orange | 14400113, set 2 | 14400139, set 3 |
Yellow | 14400114, set 2 | 14400140, set 3 |
Green | 14399984, set 5 | 14399988, set 6 |
Blue | 14400072, set 5 | 14399987, set 6 |
Pink | 14399983, set 1 | 14400128, set 7 |
Purple | 14400077, set 4 | 14399959, set 8 |
White | 14400129, set 7 | 14400081, set 9 |
Grey | 14400128, set 7 | 14399959, set 8 |
W/F | ||
Red | 14400129, set 7 | 14400081, set 9 |
Orange | 14400128, set 7 | 14399959, set 8 |
Yellow | 14400143, set 11 | 14399950, set 12 |
Green | 14400147, set 10 | 14399951, set 12 |
Blue | 14400146, set 10 | 14399947, set 11 |
Pink | 14399983, set 1 | 14400077, set 4 |
Grey | 14400113, set 2 | 14400139, set 3 |
In spring 2017 Rob Wilson manually confirmed the top-ranking ~1000 compounds of the class's SMM screen. 72 can be recognized as hits with high confidence, and in total, 134 would be worth a hypothetical secondary screen. This corresponds to hit rate of about 0.15%, low and reasonable in the Koehler Lab's viewpoint. The conserved structures Rob identified are: a 7-azaindole and a 3,4 cis pyrrolidine with a nearby tertiary amine non-aromatic heterocycle.
Ligand selections
Team | Ligand #1 (short #) | Ligand #2 (short #) |
T/R | ||
Red | 31064975 (15) | 13742946 (16) |
Orange | 83759472 (2) | 34120495 (13) |
Yellow | 83759472 (2) | 64183606 (5) |
Green | 85836506 (1) | 41507070 (11) |
Blue | 71743840 (14) | 64472564 (4) |
Pink | 47464042 (8) | 85836506 (1) |
Purple | 47464042 (8) | 63365116 (6) |
White | 62716075 (7) | 45527639 (10) |
Grey | 62716075 (7) | 64472564 (4) |
W/F | ||
Red | 41507070 (11) | 13742946 (16) |
Orange | 34297777 (12) | 64183606 (5) |
Yellow | 45714400 (9) | 31064975 (15) |
Green | 34120495 (13) | 71743840 (14) |
Blue | 65421147 (3) | 45527639 (10) |
Pink | 63365116 (6) | 45714400 (9) |
Grey | 41507070 (11) | 65421147 (3) |
M1D3: Evaluate protein purity and concentration
WF Section
BCA Assay results (A562 measurements): linked here
SDS-PAGE images:
Red Team
Orange Team
Yellow Team
Green Team
Blue Team
Pink Team
Grey Team
M1D5: PPIase assay
TR Section
Plate #1: linked here
Plate #2: linked here
WF Section
Plate #1: linked here
Plate #2: linked here
Plate #3: linked here
M1D6: DSF results
TR Section
First Derivative: linked here
Melt Curve: linked here
WF Section
First Derivative: linked here
Melt Curve: linked here
M1D7: Data analysis
Team | PPIase results | DSF results |
T/R | ||
Red | DSF red | PPiase red |
Orange | DSF orange | PPiase orange |
Yellow | DSF yellow | PPiase yellow |
Green | DSF green | PPiase green |
Blue | DSF blue | PPiase blue |
Pink | DSF pink | PPiase pink |
Purple | DSF purple | PPiase purple |
White | DSF white | PPiase white |
Platinum | DSF platinum | PPiase platinum |
W/F | ||
Red | PPIase Red | DSF Red |
Orange | PPIase Orange | DSF Orange |
Yellow | PPiase Yellow | DSF Yellow |
Green | PPIase Green | DSF Green |
Blue | PPIase Blue | DSF Blue |
Pink | PPIase Pink | DSF Pink |
Grey | PPIase Grey | DSF Grey |
Module 2: Measuring gene expression
Heatmap with ALL genes for DLD-1 & BRCA2-/-: linked here
M2D9
qPCR results
Team | qPCR Results | |
T/R | ||
Red | Sp19_qPCR_TRred | |
Orange | Sp19_qPCR_TRorange | |
Yellow | Sp19_qPCR_TRyellow | |
Green | Sp19_qPCR_TRgreen | |
Blue | Sp19_qPCR_TRblue | |
Pink | Sp19_qPCR_TRpink | |
Purple | Sp19_qPCR_TRpurple | |
White | Sp19_qPCR_TRwhite | |
Platinum | Sp19_qPCR_TRplatinum | |
W/F | ||
Red | Sp19_WFred_qPCR | |
Orange | Sp19_WForange_qPCR | |
Yellow | Sp19_WFyellow_qPCR | |
Green | Sp19_WFgreen_qPCR | |
Blue | Sp19_WFblue_qPCR | |
Pink | Sp19_WFpink_qPCR | |
Gray | Sp19_WFgrey_qPCR |
Experimental battery composition (AuNP/phage) | Weight of cathode (mg) | Actual capacity (mA*h/g) |
0 | ||
0 |
TR Battery information
Team | Experimental battery composition (AuNP/phage) | Weight of cathode (mg) | Actual capacity (mA*h/g) |
Red | |||
Orange | |||
Yellow | |||
Green | |||
Blue | |||
Pink | |||
Purple | |||
White | |||
Platinum |
WF Battery information
Team | Experimental battery composition (AuNP/phage) | Weight of cathode (mg) | Actual capacity (mA*h/g) |
Red | |||
Orange | |||
Yellow | |||
Green | |||
Blue | |||
Pink | |||
Grey |