20.109(F20): Laboratory Fundamentals of Biological Engineering
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm
| MODULE
|
DATE
|
LECTURER
|
LABORATORY EXPERIMENTS
|
ASSIGNMENTS
|
|
|
T Sept 1
|
NLL
|
Orientation and laboratory tour
|
|
|
|
| M1D1
|
R Sep 3
|
BE
|
|
Laboratory orientation quiz
Homework due
|
| M1D2
|
T Sep 8
|
BE
|
|
Homework due
|
| M1D3
|
R Sep 10
|
BE
|
|
Homework due
|
| M1D4
|
T Sep 15
|
BE
|
|
Homework due
|
| M1D5
|
R Sep 17
|
BE
|
|
Laboratory quiz
Homework due
|
| M1D6
|
T Sep 22
|
BE
|
|
Homework due
|
| M1D7
|
R Sep 24
|
BE
|
|
Homework due
|
|
|
| M2D1
|
T Sep 29
|
JN
|
|
Laboratory quiz
Homework due
|
| M2D2
|
R Oct 1
|
JN
|
|
Homework due
Data Summary draft due Sun, Oct 3 at 10 pm
[ Blog post due] Mon, Oct 4 at 10 pm
|
| M2D3
|
T Oct 6
|
JN
|
|
Homework due
|
| M2D4
|
R Oct 8
|
JN
|
|
|
Homework due
|
|
|
T/W Oct 15/16
|
|
Columbus day holiday
|
|
| 2
|
3
|
R/F Oct 17/18
|
NLL
Article Figures
Lecture slides
|
Generate gRNA plasmid
TR Prelab
WF Prelab
|
Homework due
Mini-presentation due Sat, Oct 19 at 10 pm
|
| 2
|
4
|
T/W Oct 22/23
|
NLL
Lecture slides
|
Journal club I
|
|
| 2
|
5
|
R/F Oct 24/25
|
NLL
Lecture slides
|
Confirm gRNA sequence in pgRNA
TR Prelab
WF Prelab
|
Laboratory quiz
Homework due
Data Summary revision due Sat, Oct 26 at 10 pm
|
| 2
|
6
|
T/W Oct 29/30
|
NLL
Lecture slides
|
Journal club II
|
|
| 2
|
7
|
R/F Oct/Nov 31/1
|
NLL
Lecture slides
|
Induce CRISPRi system
TR Prelab
WF Prelab
|
Homework due
Blog post due Fri, Nov 1 at 10 pm
|
| 2
|
8
|
T/W Nov 5/6
|
NLL
|
Measure fermentation products
TR Prelab
WF Prelab
|
Laboratory quiz
Homework due
|
|
|
| 3
|
1
|
R/F Nov 7/8
|
AK
Lecture slides
|
Prepare for cellular thermal shift assay
TR Prelab
WF Prelab
|
Homework due
|
|
|
|
T/W Nov 12/13
|
|
Veterans' day holiday
|
Research article due Mon, Nov 11 at 10 pm
Blog post due Tue, Nov 12 at 10 pm
|
| 3
|
2
|
R/F Nov 14/15
|
AK
Lecture slides
|
Incubate with ligand and apply heat treatment for protein denaturation
TR Prelab
WF Prelab
|
Homework due
|
| 3
|
3
|
T/W Nov 19/20
|
AK
Lecture slides
|
Begin Western blot analysis
TR Prelab
WF Prelab
|
Laboratory quiz
Homework due
|
| 3
|
4
|
R/F Nov 21/22
|
AK
Lecture slides
|
Complete antibody staining for Western blot analysis
TR Prelab
WF Prelab
|
Homework due
|
|
|
|
T/W Nov 26/27
|
|
Lecture: research proposal pitch, but no laboratory
|
|
|
|
|
R/F Nov 28/29
|
|
Thanksgiving holiday
|
|
| 3
|
5
|
T/W Dec 3/4
|
AK
Lecture slides
|
Data analysis and assignment preparation
TR Prelab
WF Prelab
|
Laboratory quiz
Homework due
|
|
|
|
R/F Dec 5/6
|
|
Research proposal presentations
|
Blog post due Fri, Dec 6 at 10 pm
|
|
|
|
|
|
T Dec 10
|
|
Feedback and celebratory lunch
|
Mini-report due Mon, Dec 9 at 10 pm
|
