Difference between revisions of "20.109(F16): Fall 2016 schedule"
MAXINE JONAS (Talk | contribs) |
|||
(60 intermediate revisions by 4 users not shown) | |||
Line 20: | Line 20: | ||
| R/F Sept 8/9 | | R/F Sept 8/9 | ||
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 Orientation lecture.pdf| Orientation lecture]] | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 Orientation lecture.pdf| Orientation lecture]] | ||
− | | [[20.109(F16):Lab tour | Orientation]]<br>[[Media: | + | | [[20.109(F16):Lab tour | Orientation]]<br>[[Media:MOD0_F16_LMM.pdf| Prelab T/R]]<br>[[Media:Fa16_M0D0_160909_marked.pdf| Prelab W/F]] |
| | | | ||
|-- | |-- | ||
Line 29: | Line 29: | ||
| T/W Sept 13/14 | | T/W Sept 13/14 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109(F16):Prepare comet chips (Day1) | Prepare microwell array and practice tissue culture]]<br> | + | | [[20.109(F16):Prepare comet chips (Day1) | Prepare microwell array and practice tissue culture]]<br>[[Media:M1D1_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M1D1_160914_marked.pdf| Prelab W/F]] |
| <font color=#04B45F>'''Lab orientation quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D1| Homework due]] | | <font color=#04B45F>'''Lab orientation quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D1| Homework due]] | ||
|-- | |-- | ||
Line 36: | Line 36: | ||
| R/F Sept 15/16 | | R/F Sept 15/16 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109(F16):Test comet chip loading variables (Day2) | Develop experiment to test loading variables and quantify growth rate]]<br> | + | | [[20.109(F16):Test comet chip loading variables (Day2) | Develop experiment to test loading variables and quantify growth rate]]<br>[[Media:M1D2_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M1D2_160916_marked.pdf| Prelab W/F]] |
|[[20.109(F16):Homework#Due_M1D2| Homework due]] | |[[20.109(F16):Homework#Due_M1D2| Homework due]] | ||
|-- | |-- | ||
Line 43: | Line 43: | ||
| T/W Sept 20/21 | | T/W Sept 20/21 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109(F16):Measure DNA damage with comet chip (Day3)| Test role of biochemical factors in genomic stability]] | + | | [[20.109(F16):Measure DNA damage with comet chip (Day3)| Test role of biochemical factors in genomic stability]]<br>[[Media:M1D3_Fa16_LMM_2.pdf| Prelab T/R]]<br>[[Media:Fa16_M1D3_160921_marked.pdf| Prelab W/F]] |
| <font color=#04B45F>'''Lab quiz'''</font color><br> [[20.109(F16):Homework#Due_M1D3| Homework due]] | | <font color=#04B45F>'''Lab quiz'''</font color><br> [[20.109(F16):Homework#Due_M1D3| Homework due]] | ||
|-- | |-- | ||
Line 57: | Line 57: | ||
| T/W Sept 27/28 | | T/W Sept 27/28 | ||
| [http://be.mit.edu/directory/noreen-lyell NLL] | | [http://be.mit.edu/directory/noreen-lyell NLL] | ||
− | | [[20.109(F16):Asses DNA repair variability with comet chip (Day4)| Query inter-individual variability in exposure susceptibility]] | + | | [[20.109(F16):Asses DNA repair variability with comet chip (Day4)| Query inter-individual variability in exposure susceptibility]]<br>[[Media:M1D4_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M1D4_160928_marked.pdf| Prelab W/F]] |
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D4| Homework due]] | | <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D4| Homework due]] | ||
|-- | |-- | ||
Line 64: | Line 64: | ||
| R/F Sept 29/30 | | R/F Sept 29/30 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109(F16):Seed cells for immuno-flourescence assay (Day5)| Develop approach for sub-nuclear visualization of DNA damage]] | + | | [[20.109(F16):Seed cells for immuno-flourescence assay (Day5)| Develop approach for sub-nuclear visualization of DNA damage]]<br>[[Media:M1D5_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M1D5_160930_marked.pdf| Prelab W/F]] |
| [[20.109(F16):Homework#Due_M1D5| Homework due]] | | [[20.109(F16):Homework#Due_M1D5| Homework due]] | ||
|-- | |-- | ||
Line 71: | Line 71: | ||
| T/W Oct 4/5 | | T/W Oct 4/5 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109(F16):Complete immuno-fluorescence assay (Day6)| Query DNA repair capacity in tumor cells]] | + | | [[20.109(F16):Complete immuno-fluorescence assay (Day6)| Query DNA repair capacity in tumor cells]]<br>[[Media:M1D6_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M1D6_161005_marked.pdf| Prelab W/F]] |
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D6| Homework due]] | | <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D6| Homework due]] | ||
|-- | |-- | ||
Line 78: | Line 78: | ||
| R/F Oct 6/7 | | R/F Oct 6/7 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109(F16):Data analysis (Day7)| Analysis of sub-nuclear foci]] | + | | [[20.109(F16):Data analysis (Day7)| Analysis of sub-nuclear foci]]<br>[[Media:M1D7_Fa16_LMM.pdf| Prelab T/R]]<br>[[Media:Fa16_M1D7_161007_marked.pdf| Prelab W/F]] |
| [[20.109(F16):Homework#Due_M1D7| Homework due]] | | [[20.109(F16):Homework#Due_M1D7| Homework due]] | ||
|-- | |-- | ||
Line 93: | Line 93: | ||
| 1 | | 1 | ||
| R/F Oct 13/14 | | R/F Oct 13/14 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL] | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D1 lecture slides.pdf|M2D1 lecture]] |
− | | [[20.109(F16):Complete in silico cloning (Day1)| Complete in silico cloning]] | + | | [[20.109(F16):Complete in silico cloning (Day1)| Complete in silico cloning]]<br>[[Media:Fa16_M2D1_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M2D1_161014_marked.pdf| Prelab W/F]] |
| [[20.109(F16):Homework#Due_M2D1| Homework due]]<br>[[20.109(F16): M1 Mini-presentation| Mini-presentation due]] Sat, Oct 15 at 10 pm | | [[20.109(F16):Homework#Due_M2D1| Homework due]]<br>[[20.109(F16): M1 Mini-presentation| Mini-presentation due]] Sat, Oct 15 at 10 pm | ||
|-- | |-- | ||
Line 100: | Line 100: | ||
| 2 | | 2 | ||
| T/W Oct 18/19 | | T/W Oct 18/19 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL] | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> [[Media:Fa16 M2D2 lecture slides.pdf|M2D2 lecture]] |
− | | [[20.109(F16):Design gRNA for CRISPRi (Day2)| Design gRNA for CRISPRi]] | + | | [[20.109(F16):Design gRNA for CRISPRi (Day2)| Design gRNA for CRISPRi]]<br>[[Media:M2D2_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M2D2_161019_marked.pdf| Prelab W/F]] |
− | + | | [[20.109(F16):Homework#Due_M2D2| Homework due]] | |
|-- | |-- | ||
| 2 | | 2 | ||
| 3 | | 3 | ||
| R/F Oct 20/21 | | R/F Oct 20/21 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL] | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D3 lecture slides.pdf|M2D3 lecture]] |
− | | [[20.109(F16):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]] | + | | [[20.109(F16):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br>[[Media:M2D3_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M2D3_161021_marked.pdf| Prelab W/F]]<br>[[Media:Fa16 M2D3 journal article discussion.pdf|Journal article discussion]] |
| [[20.109(F16):Homework#Due_M2D3| Homework due]]<br>[[20.109(F16): M1 Data Summary| Data Summary revision due]] Mon, Oct 24 at 5 pm | | [[20.109(F16):Homework#Due_M2D3| Homework due]]<br>[[20.109(F16): M1 Data Summary| Data Summary revision due]] Mon, Oct 24 at 5 pm | ||
|-- | |-- | ||
Line 114: | Line 114: | ||
| 4 | | 4 | ||
| T/W Oct 25/26 | | T/W Oct 25/26 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL] | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D4 lecture slides student.pdf|M2D4 lecture]] |
| [[20.109(F16):Journal club I (Day4)| Journal club I]] | | [[20.109(F16):Journal club I (Day4)| Journal club I]] | ||
− | | <font color=#04B45F>'''Lab quiz'''</font color><br>[http://be20109f16.blogspot.com/ Blog post due Tue, Oct 25 at 5pm | + | | <font color=#04B45F>'''Lab quiz'''</font color><br>[http://be20109f16.blogspot.com/ Blog post due] Tue, Oct 25 at 5pm |
|-- | |-- | ||
| 2 | | 2 | ||
| 5 | | 5 | ||
| R/F Oct 27/28 | | R/F Oct 27/28 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL] | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D5 lecture slides.pdf|M2D5 lecture]] |
− | | [[20.109(F16):Confirm gRNA sequence (Day5)| Confirm gRNA sequence]] | + | | [[20.109(F16):Confirm gRNA sequence (Day5)| Confirm gRNA sequence]]<br>[[Media:M2D5 Pre-Lab TR.pdf| Prelab T/R]]<br>[[Media:Fa16 M2D5 EmilyClark 161027 pptx.pdf| Prelab W/F]] |
| [[20.109(F16):Homework#Due_M2D5| Homework due]] | | [[20.109(F16):Homework#Due_M2D5| Homework due]] | ||
|-- | |-- | ||
Line 128: | Line 128: | ||
| 6 | | 6 | ||
| T/W Nov 1/2 | | T/W Nov 1/2 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL] | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D6 lecture slides.pdf|M2D6 lecture]] |
| [[20.109(F16):Journal club II (Day 6) | Journal club II]] | | [[20.109(F16):Journal club II (Day 6) | Journal club II]] | ||
| <font color=#04B45F>'''Lab quiz'''</font color><br> | | <font color=#04B45F>'''Lab quiz'''</font color><br> | ||
Line 135: | Line 135: | ||
| 7 | | 7 | ||
| R/F Nov 3/4 | | R/F Nov 3/4 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL] | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D7 lecture slides.pdf|M2D7 lecture]] |
− | | [[20.109(F16):Induce CRISPRi system (Day7)| Induce CRISPRi system]] | + | | [[20.109(F16):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br>[[Media:M2D7_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M2D7_161104_marked.pdf| Prelab W/F]] |
| [[20.109(F16):Homework#Due_M2D7| Homework due]] | | [[20.109(F16):Homework#Due_M2D7| Homework due]] | ||
|-- | |-- | ||
Line 142: | Line 142: | ||
| 8 | | 8 | ||
| T/W Nov 8/9 | | T/W Nov 8/9 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL] | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D8 lecture slides.pdf|M2D8 lecture]] |
− | + | | [[20.109(F16)::Measure_fermentation_products_(Day8) | Measure fermentation products]]<br>[[Media:M2D8_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M2D8_161109_marked.pdf| Prelab W/F]] | |
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M2D8| Homework due]] | | <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M2D8| Homework due]] | ||
|-- | |-- | ||
Line 159: | Line 159: | ||
| T/W Nov 15/16 | | T/W Nov 15/16 | ||
| [http://be.mit.edu/directory/angela-belcher AB] | | [http://be.mit.edu/directory/angela-belcher AB] | ||
− | | [[20.109(F16):Growth of phage materials (Day1)| Growth of phage materials]] | + | | [[20.109(F16):Growth of phage materials (Day1)| Growth of phage materials]]<br>[[Media:Fa16 M3D1 prelab slides.pdf| Prelab T/R]]<br>[[Media:Fa16_M3D1_161116_marked.pdf| Prelab W/F]] |
| [[20.109(F16):Homework#Wrapping_up_M2| Homework due]] <br> | | [[20.109(F16):Homework#Wrapping_up_M2| Homework due]] <br> | ||
|-- | |-- | ||
Line 166: | Line 166: | ||
| R/F Nov 17/18 | | R/F Nov 17/18 | ||
| [http://be.mit.edu/directory/angela-belcher AB] | | [http://be.mit.edu/directory/angela-belcher AB] | ||
− | | [[20.109(F16):Purify active materials (Day2)| Purify active materials]] | + | | [[20.109(F16):Purify active materials (Day2)| Purify active materials]]<br>[[Media:Fa16_M3D2_LMM.pdf| Prelab T/R]]<br>[[Media:Fa16_M3D2_161118_marked.pdf| Prelab W/F]] |
− | | [[20.109(F16):Homework#Due_M3D2| Homework due]]<br>[http://be20109f16.blogspot.com/ Blog post due | + | | [[20.109(F16):Homework#Due_M3D2| Homework due]]<br>[[20.109(F16): Research article| Research article due]] Sun, Nov 20 at 5 pm <br>[http://be20109f16.blogspot.com/ Blog post due] Mon, Nov 21 at 5pm |
|-- | |-- | ||
| | | | ||
| | | | ||
| T/W Nov 22/23 | | T/W Nov 22/23 | ||
− | | | + | | |
| <font color = #FF0000>Lecture, but no laboratory</font color> | | <font color = #FF0000>Lecture, but no laboratory</font color> | ||
| | | | ||
Line 187: | Line 187: | ||
| T/W Nov 29/30 | | T/W Nov 29/30 | ||
| [http://be.mit.edu/directory/angela-belcher AB] | | [http://be.mit.edu/directory/angela-belcher AB] | ||
− | | [[20.109(F16):Cathode construction (Day3)| Cathode construction]] | + | | [[20.109(F16):Cathode construction (Day3)| Cathode construction]]<br>[[Media:Fa16_M3D3_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M3D3_161130_marked.pdf| Prelab W/F]] |
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M3D3| Homework due]] | | <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M3D3| Homework due]] | ||
|-- | |-- | ||
Line 194: | Line 194: | ||
| R/F Dec 1/2 | | R/F Dec 1/2 | ||
| [http://be.mit.edu/directory/angela-belcher AB] | | [http://be.mit.edu/directory/angela-belcher AB] | ||
− | | [[20.109(F16):TEM (Day4)| TEM]] | + | | [[20.109(F16):TEM (Day4)| TEM]]<br>[[Media:Fa16_M3D4_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M3D4_161202_marked.pdf| Prelab W/F]] |
| [[20.109(F16):Homework#Due_M3D4| Homework due]] | | [[20.109(F16):Homework#Due_M3D4| Homework due]] | ||
|-- | |-- | ||
Line 201: | Line 201: | ||
| T/W Dec 6/7 | | T/W Dec 6/7 | ||
| [http://be.mit.edu/directory/angela-belcher AB] | | [http://be.mit.edu/directory/angela-belcher AB] | ||
− | | [[20.109(F16):Battery assembly and testing (Day5)| Battery assembly and testing]] | + | | [[20.109(F16):Battery assembly and testing (Day5)| Battery assembly and testing]]<br>[[Media:Fa16_M3D5_LMM.pdf| Prelab T/R]]<br>[[Media:Fa16_M3D5_161207_marked.pdf| Prelab W/F]] |
− | | <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due (after) M3D5| Homework due]]<br>[http://be20109f16.blogspot.com/ Blog post due Wed, Dec 7 at 10pm | + | | <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due (after) M3D5| Homework due]]<br>[http://be20109f16.blogspot.com/ Blog post due] Wed, Dec 7 at 10pm |
|-- | |-- | ||
| 3 | | 3 | ||
Line 218: | Line 218: | ||
| | | | ||
| <font color = #00BFFF>'''Feedback and celebratory lunch'''</font color> | | <font color = #00BFFF>'''Feedback and celebratory lunch'''</font color> | ||
− | | [[20.109(F16): Biomaterials engineering mini-report | Biomaterials engineering mini-report due]] Mon, Dec 12 at 10 pm <br>[http://be20109f16.blogspot.com/ Blog post due Wed, Dec 14 at 10pm | + | | [[20.109(F16): Biomaterials engineering mini-report | Biomaterials engineering mini-report due]] Mon, Dec 12 at 10 pm <br>[http://be20109f16.blogspot.com/ Blog post due] Wed, Dec 14 at 10pm |
|} | |} | ||
</div> | </div> |
Latest revision as of 18:39, 19 December 2016
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
Schedule details:
Lecture: T/R 11-12 (16-220)
Laboratory sections: T/R 1-5 or W/F 1-5 (56-322)