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• DNA Melting Part 1: Measuring Temperature and Fluorescence
  ...as some shortcomings, it will give you a good idea of how all of the parts work together to make melting curves. The instrument includes a resistance tempe ...his lab. You will probably notice some shortcomings your instrument as you work. Don't worry about that — in part 2, you will make many improvements
  27 KB (4,497 words) - 17:49, 19 April 2017
• DNA Melting Part 2: Lock-in Amplifier and Temperature Control
  ...gher frequency. So rip that sucker off — now your amplifier needs to work on a signal that is changing much more quickly. ...ou may need to update the gains of one or both of your amplifier stages to work best with the lower signal level and increased frequency.
  20 KB (3,227 words) - 20:05, 7 November 2017
• 20.109(S17): Spring 2017 schedule
  ... at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of
  11 KB (1,661 words) - 19:53, 10 May 2017
• DNA Melting: Model function and parameter estimation by nonlinear regression
  ...th state variables. Because of this assumption, lumped element models only work well if the bodies you are modeling have a nearly uniform temperature. Comp
  18 KB (2,803 words) - 21:55, 17 August 2017
• 20.109(S17): Fall 2016 schedule
  ... at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of
  2 KB (353 words) - 16:25, 27 December 2016
• Assignment 8, Part 2: lock-in amplifier and temperature control
  ...gher frequency. So rip that sucker off — now your amplifier needs to work on a signal that is changing much more quickly. ...ou may need to update the gains of one or both of your amplifier stages to work best with the lower signal level and increased frequency.
  17 KB (2,813 words) - 22:56, 7 November 2017
• DNA Melting Report Requirements
  ...your apparatus with sufficient detail for another person to replicate your work. Assume the reader is familiar with the concepts of 20.309 and has access t
  6 KB (864 words) - 18:50, 16 April 2017
• Python:Simulating DNA Melting
  ===Hi! I work, even though there's no writeup!===
  12 KB (1,459 words) - 10:25, 24 May 2011
• Lab Manual: Optical Microscopy
  ... in the lab. The work is divided into 3 parts. Each part requires some lab work, some analysis, lots of clear thinking, and a written report. You will subm
  23 KB (3,767 words) - 15:19, 10 January 2017
• Optical Microscopy Week 3: Experiments
  ...iments of J. Perrin that lead to his Nobel Prize. A translation of the key work is included in the reprints folder. Have a look – he used latex spheres, ...a [http://en.wikipedia.org/wiki/Motor_protein motor protein] that does the work. The motor molecule uses energy from the hydrolysis of one ATP molecule to
  15 KB (2,322 words) - 17:14, 18 September 2012
• Electronics primer
  ...he plus terminal to the minus terminal. In most of the components you will work with, such as wires, capacitors, and resistors, the charge carriers are neg
  22 KB (3,534 words) - 13:50, 19 October 2018
• 20.309:Lab Report Guidelines
  Some lab exercises involve significant design and construction work. Others utilize complex systems that were built by others. In either case, ...y with other students. '''''The report you submit must consist entirely of work of done by members of your lab group. Any words, data, images, code, or oth
  10 KB (1,534 words) - 21:33, 13 March 2017
• 20.309:Course Information
  [[Category:Needs review]] ...ses can be completed in about 6 hours per week. Some students find the lab work significantly more or less time consuming. If you have little experience bu
  5 KB (714 words) - 16:37, 7 February 2020
• 20.309:Safety
  ...Also, the blinking laser safety sign near the door must be turned any time work with these lasers is taking place. Needless to say, purposefully aiming the ...risk of electrical shock from the low voltage electrical circuits you will work with in 20.309.
  5 KB (821 words) - 16:50, 10 September 2015
• 20.309:Learning outcomes
  h. Demonstrate conscientious work habits
  5 KB (658 words) - 02:45, 7 July 2010
• Lab Manual: Atomic Force Microscopy (AFM)
  #Work with a TA or Instructor. To be useful for imaging, an AFM needs to scan its probe over the sample surface. Our microscopes are designed wit
  56 KB (9,320 words) - 20:42, 13 December 2017
• 20.109(S16):Induce protein expression (Day5)
  As evidenced by Nagai’s work, wild-type inverse pericam is not toxic to BL21(DE3)pLysS cells. Although i ...u induce your cells with IPTG, you will let the protein factories do their work for 2-3 hours. During this time, you will evaluate the DNA from your two X#
  16 KB (2,609 words) - 21:55, 23 February 2016
• ThorLabs OTKB optical trapping kit
  ...ve for the trap stiffness, the variance in position is the only thing that needs to be calculated. Since this variance is measured in volts, position calibr ...so TransferFunc would be saved as TransferFunc.m. Otherwise, they will not work).:
  24 KB (4,010 words) - 15:32, 5 August 2010
• Problem Set 2 Fall 2010
  Draw a ray diagram to justify your answer. A ruler will help keep your work tidy. (Hint: will any of the rays stay the same?) ...ems do you think the fish uses for underwater vision? Why does this system work better below water? Draw a few rays and/or include a couple of bullet point
  4 KB (740 words) - 02:53, 15 September 2010
• Procedure: Diffusion in biological gels
  ... glancing angle, using the bench ligths for illuination. If this does not work, ask a TA or Instructor to help you.
  5 KB (878 words) - 23:26, 5 October 2010
• 20.345:Course Information
  ...ity to pursue a specific technique in depth. You will have many chances to work on your writing, presentation, and project planning skills. Active particip ...Developing an entirely new in vitro neuronal guidance technology is folly. Work closely with your mentor to find the right balance. The lab has a modest bu
  11 KB (1,722 words) - 17:22, 6 February 2018
• 20.345:Final project proposal
  ...uld detail what you are planning to do, the motivation for doing it, prior work, and relevant theory. It should include a comprehensive development plan an ...m line on motivation, goal, key milestones, risks, resources, and previous work.
  2 KB (283 words) - 00:40, 12 February 2014
• Spring 2011:CellFinder
  *we have little electronics experience, and Jenn needs an electronics review :) ****this didn't work either because the polymer is too hydrophobic or it is blocked
  4 KB (610 words) - 00:07, 3 April 2011
• Spring 11:QRT-PCR
  ...ize the current needed to heat the wire, while still being thick enough to work with. The thermal epoxy ensures that the resistance wire does not make cont We definitely see less smearing with newer version. There is of course much work still to do.
  8 KB (1,345 words) - 19:36, 19 May 2011
• Spring 11:QRT-PCR weekly reports
  * ordered parts for heated lid may not work, or may not come in for a while * PCR may not work even with heated lid - will need to think of other ways to fix this problem
  3 KB (447 words) - 04:09, 8 April 2011
• Weekly Updates
  - Work will start with the image analysis programming, including the analysis of c ...on seems to be adapting the QuickPALM code available in open source to our needs
  9 KB (1,454 words) - 20:19, 19 May 2011
• Spring 11:Chemotaxis Assay
  * needs Power of .5W (12V-DC, 0.04 Amps) ... precise control of the fluid (chemoeffector band width and stoppped flow) needs to be improved.
  14 KB (2,196 words) - 23:14, 18 May 2011
• DNA Melting: Simulating DNA Melting - Basics
  If you prefer to work in Python, please see [[Python:Simulating DNA Melting|this page]]. Does the <tt>DnaFraction</tt> function work as expected? A good way to test the function is to plot a melting curve gen
  13 KB (2,026 words) - 22:46, 15 April 2018
• Spring 11:CellPrinter
  Work performed and risks identified along the way.
  519 B (60 words) - 19:38, 19 May 2011
• March14
  ===Work Performed===
  125 B (15 words) - 16:51, 30 March 2011
• QRT-PCR:Heated Lid
  ...nd silicone tape sandwiched between two plates of aluminum...but it didn't work too well. They said it "smoked and burned out." However, they were designin
  3 KB (571 words) - 19:07, 4 April 2011
• Spring 11:Directed Evolution/Week of 4-04-2011 Status
  ...hernet Controller]. We now have control via RS232 (couldn't get TCP/IP to work)
  925 B (132 words) - 20:52, 15 May 2011
• QRT-PCR:Get Started
  This page is designed to be useful for whoever is just starting to work on the 20.309/345 QRT-PCR machine.
  901 B (144 words) - 23:03, 4 May 2011
• Spring 11:Directed Evolution/Week of 5-09-2011 Status
  Also attempted to pour some agar plugs which didn't work out very well.
  792 B (113 words) - 03:32, 15 May 2011
• Weekly Progress Reports
  ===Work=== ===Work===
  5 KB (849 words) - 08:32, 19 May 2011
• Spring 11:QRT-PCR: Progress Notes
  ...roke. The lid is not hooked up, but it is ready. I wish I had been able to work faster and do more, but... I'll be back tomorrow. ...iew code. I will be working with versions 2.700 and later. Not all of them work. I will take notes on this; take a look at [[QRT-PCR:LabView-versions]].
  7 KB (1,228 words) - 16:48, 19 May 2011
• Confocal Wiki
  ... confocal microscope in the 20.309 laboratory. Stephanie Bachar continued work on the microscope during January of 2011. When beginning our project, the
  21 KB (3,664 words) - 21:38, 4 June 2015
• Theory
  ... the camera in each photoswitching cycle. This calculation is based on the work of Thompson et al. where a detailed analysis of single molecule localizatio
  9 KB (1,442 words) - 20:08, 19 May 2011
• Cartridge Manipulation
  ... After tapping the screwdriver into the groove, the cover would gradually work its way off, though cracks would occur in the plastic around the top edge.
  7 KB (1,060 words) - 13:29, 19 May 2011
• Photobleaching Model
  ...er for us to use? (Kaduk says that a nonpolar polymer gel-like thing might work a lot better than trying to find glycerol-plus.) Of course, diffusion is ab A thought about the glycerol thing -- why doesn't this work with gels? Or does it? Viscosity is not the same as gelification, I suppose
  11 KB (1,755 words) - 23:31, 18 July 2011
• Reference List
  Below is a list of the most relevant references used for this work:
  2 KB (233 words) - 20:37, 19 May 2011
• DNA Melter Improvements
  '''Getting it to work with the old photodiode''' ... of code. TODO: see if fitting can be sped up. TODO: see if animation will work on Athena or 309 lab computers.
  11 KB (1,864 words) - 21:20, 22 July 2011
• Lab Manual:Introduction to electronics
  ...ith electronics and electrical measurement equipment, you may only need to work through a few of the sections in the lab manual. However, you will at least
  1 KB (236 words) - 18:58, 13 August 2013
• Limits of Detection:Report Requirements
  ...rything in your report, including computer code, must be entirely your own work.'''''
  2 KB (363 words) - 20:20, 10 April 2014
• Status Report Interrogatories (Spring 2012)
  Diligent record keeping is a vital component of superior lab work. For hundreds of years, lab notebooks looked like like the example at righ ...here proactive or responsive things you should do differently, to maximize work with others?
  4 KB (610 words) - 18:44, 13 August 2013
• Course Journal -- Nathan S Lachenmyer
  ... to instrumentation that make the difference in enabling researchers to do work that would otherwise be outside of their budget. ** If this doesn't work, restart Matlab
  3 KB (464 words) - 21:52, 27 February 2012
• Spring 2012:Leanna Morinishi Lab 1
  ...a is one example, taken from tethered bead data at 20 mW. This method will work best with a tethered bead stuck in the trap at an oscillation amplitude > t
  13 KB (2,020 words) - 19:56, 9 March 2012
• Spring 2012:PSF lab
  ...ect such info in the current wiki page. In particular, the ImageJ tutorial needs to be corrected.
  680 B (90 words) - 18:33, 15 August 2013
• Spring 2012:Leanna Morinishi Journal
  ...here proactive or responsive things you should do differently, to maximize work with others? ...s definitely affect my work ethic. I can be a quick learner, I can do good work, but if my heart's not in it, quality tanks.
  14 KB (2,366 words) - 04:50, 17 May 2012
• A.Williams: Objectives and Literature Review
  I have decided to work with SLM's for my final project because they are extremely useful devices t
  7 KB (1,162 words) - 07:22, 14 March 2012