Difference between revisions of "20.109(S23):Spring 2023 schedule"
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| Line 52: | Line 52: | ||
| M1D4 | | M1D4 | ||
| R/F Feb 23/24 | | R/F Feb 23/24 | ||
| − | | [http://be.mit.edu/directory/angela-koehler AK] <br> | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L4 2023.pdf| Lecture slides]] |
| − | | [[20.109(S23):M1D4 |Confirm purified protein using Western blot]] <br> | + | | [[20.109(S23):M1D4 |Confirm purified protein using Western blot]] <br> [[Media:Sp23 M1D4 nll.pdf| TR Prelab slides]]<br> [[Media:Sp23 M1D4 jz.pdf| WF Prelab slides]] |
| <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D4|Homework due]] | | <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D4|Homework due]] | ||
|-- | |-- | ||
| M1D5 | | M1D5 | ||
| T/W Feb/Mar 28/1 | | T/W Feb/Mar 28/1 | ||
| − | | [http://be.mit.edu/directory/angela-koehler AK] <br> | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L5 2023.pdf| Lecture slides]] |
| − | | [[20.109(S23):M1D5 |Image Western blot of purified protein]] <br> | + | | [[20.109(S23):M1D5 |Image Western blot of purified protein]] <br> [[Media:Sp23 M1D5 nll.pdf| TR Prelab slides]] <br> [[Media:Sp23 M1D5 jz_modded.pdf| WF Prelab slides]] <br> [[Media:Sp23_M1D5_methods_exercise.pdf | Methods Activity]] |
| [[20.109(S23):Homework#Due_M1D5|Homework due]] <br> | | [[20.109(S23):Homework#Due_M1D5|Homework due]] <br> | ||
|-- | |-- | ||
| M1D6 | | M1D6 | ||
| R/F Mar 2/3 | | R/F Mar 2/3 | ||
| − | | [http://be.mit.edu/directory/angela-koehler AK] <br> | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L6 2023.pdf| Lecture slides]] |
| − | | [[20.109(S23):M1D6 |Prepare and scan small molecule microarray (SMM) slides]] <br> | + | | [[20.109(S23):M1D6 |Prepare and scan small molecule microarray (SMM) slides]] <br> [[Media:Sp23 M1D6 nll.pdf| TR Prelab slides]] <br> [[Media:Sp23 M1D6 jz.pdf| WF Prelab slides]] |
| [[20.109(S23):Homework#Due_M1D6|Homework due]] <br> [[20.109(S23):Research talk| <font color = #b3251c>'''Research talk due'''</font color>]] Sat, Mar 4 at 10pm <br> | | [[20.109(S23):Homework#Due_M1D6|Homework due]] <br> [[20.109(S23):Research talk| <font color = #b3251c>'''Research talk due'''</font color>]] Sat, Mar 4 at 10pm <br> | ||
|-- | |-- | ||
| M1D7 | | M1D7 | ||
| T/W Mar 7/8 | | T/W Mar 7/8 | ||
| − | | [http://be.mit.edu/directory/angela-koehler AK] <br> | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L7 2023.pdf| Lecture slides]] |
| − | | [[20.109(S23):M1D7 |Analyze SMM data to identify putative small molecule binders]] <br> | + | | [[20.109(S23):M1D7 |Analyze SMM data to identify putative small molecule binders]] <br> [[Media:Sp23 M1D7 bcm.pdf | T/R Prelab slides]] <br> [[Media:Sp23 M1D7 jz.pdf | W/F Prelab slides]] |
| [[20.109(S23):Homework#Due_M1D7|Homework due]] | | [[20.109(S23):Homework#Due_M1D7|Homework due]] | ||
|-- | |-- | ||
| Line 77: | Line 77: | ||
| R/F Mar 9/10 | | R/F Mar 9/10 | ||
| BE Comm Lab <br> | | BE Comm Lab <br> | ||
| − | | [[20.109(S23):M1D8 |Organize Data summary figures and results]] <br> | + | | [[20.109(S23):M1D8 |Organize Data summary figures and results]] <br> [[Media:Sp23 M1D8 nll.pdf| TR Prelab slides]] <br> [[Media:Sp23 M1D8 jz.pdf| WF Prelab slides]] |
| <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D8|Homework due]] | | <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D8|Homework due]] | ||
|-- | |-- | ||
| M2D1 | | M2D1 | ||
| T/W Mar 14/15 | | T/W Mar 14/15 | ||
| − | | [http://be.mit.edu/directory/becky-meyer BCM] <br> | + | | [http://be.mit.edu/directory/becky-meyer BCM] <br> [[Media:20.109 Sp23 M2L1.pdf| Lecture slides]] |
| − | | [[20.109(S23):M2D1 |Determine mutagenesis strategy]] <br> | + | | [[20.109(S23):M2D1 |Determine mutagenesis strategy]] <br> [[Media:Sp23 M2D1 nll.pdf| TR Prelab slides]] <br> [[Media:Sp23 M2D1 jz.pdf| WF Prelab slides]] |
| [[20.109(S23):Homework#Due_M2D1|Homework due]] | | [[20.109(S23):Homework#Due_M2D1|Homework due]] | ||
|-- | |-- | ||
| M2D2 | | M2D2 | ||
| R/F Mar 16/17 | | R/F Mar 16/17 | ||
| − | | [http://be.mit.edu/directory/becky-meyer BCM] <br> | + | | [http://be.mit.edu/directory/becky-meyer BCM] <br> [[Media:20.109 Sp23 M2L2.pdf| Lecture slides]] |
| − | | [[20.109(S23):M2D2 |Perform site-directed mutagenesis]] <br> | + | | [[20.109(S23):M2D2 |Perform site-directed mutagenesis]] <br> [[Media:Sp23 M2D2.pdf| TR Prelab slides]] <br> [[Media:Sp23 M2D2.pdf| WF Prelab slides]] |
| [[20.109(S23):Homework#Due_M2D2|Homework due]] <br> [[20.109(S23):Data Summary| <font color = #b3251c>'''Data Summary draft due'''</font color>]] Sat, Mar 18 at 10 pm <br> [https://app.slack.com/client/T01KU1VLZQB/C04M5JH7XBL Blog post due] Mon, Mar 20 at 10 pm | | [[20.109(S23):Homework#Due_M2D2|Homework due]] <br> [[20.109(S23):Data Summary| <font color = #b3251c>'''Data Summary draft due'''</font color>]] Sat, Mar 18 at 10 pm <br> [https://app.slack.com/client/T01KU1VLZQB/C04M5JH7XBL Blog post due] Mon, Mar 20 at 10 pm | ||
|-- | |-- | ||
| M2D3 | | M2D3 | ||
| T/W Mar 21/22 | | T/W Mar 21/22 | ||
| − | | [http://be.mit.edu/directory/becky-meyer BCM] <br> | + | | [http://be.mit.edu/directory/becky-meyer BCM] <br> [[Media:20.109 Sp23 M2L3.pdf| Lecture slides]] |
| − | | [[20.109(S23):M2D3 |Sequence clones and transform into yeast cells]] <br> | + | | [[20.109(S23):M2D3 |Sequence clones and transform into yeast cells]] <br> [[Media:Sp23 M2D3 nll.pdf| TR Prelab slides]] <br> [[Media:Sp23 M2D3 jz.pdf| WF Prelab slides]] |
| [[20.109(S23):Homework#Due_M2D3|Homework due]] <br> | | [[20.109(S23):Homework#Due_M2D3|Homework due]] <br> | ||
|-- | |-- | ||
| M2D4 | | M2D4 | ||
| R/F Mar 23/24 | | R/F Mar 23/24 | ||
| − | | [http://be.mit.edu/directory/becky-meyer BCM] <br> | + | | [http://be.mit.edu/directory/becky-meyer BCM] <br> [[Media:20.109 Sp23 M2L4.pdf| Lecture slides]] |
| − | | [[20.109(S23):M2D4 |Determine transporter mutation and expression experiment]] <br> | + | | [[20.109(S23):M2D4 |Determine transporter mutation and prepare expression experiment]] <br> [[Media:Sp23 M2D4 nll.pdf|TR Prelab slides]] <br> [[Media:Sp23 M2D4 jz.pdf|WF Prelab slides]] |
| <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M2D4|Homework due]] | | <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M2D4|Homework due]] | ||
|-- | |-- | ||
| Line 124: | Line 124: | ||
| M2D5 | | M2D5 | ||
| T/W Apr 11/12 | | T/W Apr 11/12 | ||
| − | | [http://be.mit.edu/directory/becky-meyer BCM] <br> | + | | [http://be.mit.edu/directory/becky-meyer BCM] <br> [[Media:20109 Sp23 M2L5.pdf|Lecture slides]] |
| − | | [[20.109(S23):M2D5 | Immunofluorescence staining]] <br> | + | | [[20.109(S23):M2D5 | Immunofluorescence staining]] <br> [[Media:Sp23 M2D5 bcm.pdf|TR Prelab slides]] <br> [[Media:Sp23 M2D5 jz.pdf|WF Prelab slides]] |
| [[20.109(S23):Homework#Due_M2D5|Homework due]] | | [[20.109(S23):Homework#Due_M2D5|Homework due]] | ||
|-- | |-- | ||
| M2D6 | | M2D6 | ||
| R/F Apr 13/14 | | R/F Apr 13/14 | ||
| − | | [http://be.mit.edu/directory/becky-meyer BCM] <br> | + | | [http://be.mit.edu/directory/becky-meyer BCM] <br> [[Media:20.109Sp23 M2L6.pdf|Lecture slides]] |
| − | | [[20.109(S23):M2D6 | Analyze expression data and prepare metal uptake experiment]] <br> | + | | [[20.109(S23):M2D6 | Analyze expression data and prepare metal uptake experiment]] <br> [[Media:Sp23 M2D6 bcm.pdf|TR Prelab slides]] <br> [[Media:Sp23 M2D6 jz.pdf|WF Prelab slides]] |
| [[20.109(S23):Homework#Due_M2D5|Homework due]] | | [[20.109(S23):Homework#Due_M2D5|Homework due]] | ||
|-- | |-- | ||
| Line 142: | Line 142: | ||
| M2D7 | | M2D7 | ||
| R/F Apr 20/21 | | R/F Apr 20/21 | ||
| − | | [http://be.mit.edu/directory/becky-meyer BCM] <br> | + | | [http://be.mit.edu/directory/becky-meyer BCM] <br> [[Media:20.109 Sp23 M2L7.pdf|Lecture slides]] |
| − | | [[20.109(S23):M2D7 |Analyze ICP-OES data and examine yeast tolerance to metal]] <br> | + | | [[20.109(S23):M2D7 |Analyze ICP-OES data and examine yeast tolerance to metal]] <br> [[Media:Sp23 M2D7 bcm.pdf|TR Prelab slides]] <br> [[Media:Sp23 M2D7 jz.pdf|WF Prelab slides]] |
| [[20.109(S23):Homework#Due_M2D7|Homework due]] | | [[20.109(S23):Homework#Due_M2D7|Homework due]] | ||
|-- | |-- | ||
| Line 149: | Line 149: | ||
| T/W Apr 25/26 | | T/W Apr 25/26 | ||
| BE Comm Lab <br> | | BE Comm Lab <br> | ||
| − | | [[20.109(S23):M2D8 |Complete data analysis and organize Research article figures]] <br> | + | | [[20.109(S23):M2D8 |Complete data analysis and organize Research article figures]] <br> [[Media:Sp23 M2D8 nll.pdf| TR Prelab slides]]<br> [[Media:Sp23 M2D8 jz.pdf| WF Prelab slides]]<br> [[Media:RA discussion bcm.pdf|RA discussion]] |
| <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M2D8|Homework due]] | | <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M2D8|Homework due]] | ||
|-- | |-- | ||
| M3D1 | | M3D1 | ||
| R/F Apr 27/28 | | R/F Apr 27/28 | ||
| − | | [http://be.mit.edu/directory/becky-meyer BCM]<br> | + | | [http://be.mit.edu/directory/becky-meyer BCM]<br> [[Media:Sp23 Drug discovery ethics exercise.pdf|Discussion slides]] |
| − | | [[20.109(S23):M3D1 |Brainstorm ideas for Research proposal presentation]] <br> | + | | [[20.109(S23):M3D1 |Brainstorm ideas for Research proposal presentation]] <br> [[Media:Sp23 M3D1 nll.pdf|TR Prelab slides]]<br> [[Media:Sp23 M3D1 jz.pdf|WF Prelab slides]] |
| [[20.109(S23):Homework#Due_M3D1|Homework due]] <br> | | [[20.109(S23):Homework#Due_M3D1|Homework due]] <br> | ||
|-- | |-- | ||
| Line 161: | Line 161: | ||
| T/W May 2/3 | | T/W May 2/3 | ||
| [http://be.mit.edu/directory/angela-belcher AB]<br> | | [http://be.mit.edu/directory/angela-belcher AB]<br> | ||
| − | | [[20.109(S23):M3D2 |Pitch research proposal presentation ideas ]] <br> | + | | [[20.109(S23):M3D2 |Pitch research proposal presentation ideas ]] <br> [[Media:Sp23 M3D2 nll.pdf| TR Prelab slides]] <br> [[Media:Sp23 M3D2 jz.pdf| WF Prelab slides]] |
| [[20.109(S23):Research article| <font color = #b3251c>'''Research article due'''</font color>]] Mon, May 1 at 10 pm <br> [https://app.slack.com/client/T01KU1VLZQB/C04M5JH7XBL Blog post due] Tue, May 2 at 10 pm | | [[20.109(S23):Research article| <font color = #b3251c>'''Research article due'''</font color>]] Mon, May 1 at 10 pm <br> [https://app.slack.com/client/T01KU1VLZQB/C04M5JH7XBL Blog post due] Tue, May 2 at 10 pm | ||
|-- | |-- | ||
| Line 167: | Line 167: | ||
| R/F May 4/5 | | R/F May 4/5 | ||
| BE Comm Lab | | BE Comm Lab | ||
| − | | [[20.109(S23):M3D3 |Develop ideas for Research proposal presentation ]] <br> | + | | [[20.109(S23):M3D3 |Develop ideas for Research proposal presentation ]] <br> [[Media:Sp23 M3D3 nll.pdf| TR Prelab slides]]<br>[[Media:Sp23 M3D3 nll.pdf| WF Prelab slides]] |
| [[20.109(S23):Homework#Due_M3D3|Homework due]]<br> | | [[20.109(S23):Homework#Due_M3D3|Homework due]]<br> | ||
|-- | |-- | ||
Latest revision as of 16:51, 5 May 2023
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not enough time to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: Tuesday (T) and Thursday (R) 11 - 12 pm in 1-390
Laboratory section times: Tuesday (T) and Thursday (R) 1 - 5 pm or Wednesday (W) and Friday (F) 1 - 5 pm in 56-322
ABSENCE POLICY:
Absences from lecture: Attendance will be recorded for participation points throughout the semester. If absent, student is responsible for all information provided in lecture.
Absences from laboratory: Excused absences should be discussed with the Instructors as soon as possible. Because make-up laboratory time is not provided, attendance in another section may be required to complete the necessary experiments. Unexcused absences will result in a 1/3 of a letter grade deduction from the final grade on the major assignment for the module (for example, a B+ would become a B).
