Difference between revisions of "20.109(S23):Spring 2023 schedule"
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<font color= #015526 >'''SCHEDULE DETAILS:'''</font color><br> | <font color= #015526 >'''SCHEDULE DETAILS:'''</font color><br> | ||
| − | <font color= # | + | <font color= #0aa24d >'''Lecture times:'''</font color> Tuesday (T) and Thursday (R) 11 - 12 pm in 1-390<br> |
| − | <font color= # | + | <font color= #0aa24d >'''Laboratory section times:'''</font color> Tuesday (T) and Thursday (R) 1 - 5 pm or Wednesday (W) and Friday (F) 1 - 5 pm in 56-322<br> |
<font color= #015526 >'''ABSENCE POLICY:'''</font color><br> | <font color= #015526 >'''ABSENCE POLICY:'''</font color><br> | ||
| − | <font color= # | + | <font color= #0aa24d >'''Absences from lecture:'''</font color> Attendance will be recorded for participation points throughout the semester. If absent, student is responsible for all information provided in lecture. <br> |
| − | <font color= # | + | <font color= #0aa24d >'''Absences from laboratory:'''</font color> Excused absences should be discussed with the Instructors as soon as possible. Because make-up laboratory time is not provided, attendance in another section may be required to complete the necessary experiments. Unexcused absences will result in a 1/3 of a letter grade deduction from the final grade on the major assignment for the module (for example, a B+ would become a B). |
{| border=1px | {| border=1px | ||
| Line 21: | Line 21: | ||
|-- | |-- | ||
| | | | ||
| − | | | + | | T/W Feb 7/8 |
| − | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> [[Media: | + | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> [[Media:Sp23 Orientation lecture student.pdf| Lecture slides]] <br> |
| − | | [[20.109( | + | | [[20.109(S23):Laboratory tour | Orientation and laboratory tour]]<br> [[Media:Sp23 M0 nll.pdf| TR Prelab slides]] <br> [[Media:Sp23_M0_jz.pdf| WF Prelab slides]]<br> [[Media:20.109 updated EHS slides.pdf| EHS slides]] |
| | | | ||
|-- | |-- | ||
| M1D1 | | M1D1 | ||
| − | | | + | | R/F Feb 9/10 |
| − | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L1 2023.pdf| Lecture slides]] |
| − | | [[20.109( | + | | [[20.109(S23):M1D1 | Complete in-silico cloning of protein expression plasmid]]<br> [[Media:Sp23 M1D1 nll.pdf| TR Prelab slides]] <br> [[Media:Sp23_M1D1_jz.pdf | WF Prelab slides]] <br> |
| − | | <font color= # | + | | <font color= #0aa24d >'''Orientation quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D1|Homework due]] |
|-- | |-- | ||
| M1D2 | | M1D2 | ||
| − | | | + | | T/W Feb 14/15 |
| − | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L2 2023.pdf| Lecture slides]] |
| − | | [[20.109( | + | | [[20.109(S23):M1D2 |Perform protein purification protocol]] <br> [[Media:Sp23 M1D2 nll.pdf| TR Prelab slides]] <br> [[Media:Sp23 M1D2 jz.pdf| WF Prelab slides]] <br> [[Media:Sp23 Background&Motivation slides.pdf| Background & Motivation slides]] |
| − | | [[20.109( | + | | [[20.109(S23):Homework#Due_M1D2|Homework due]] |
|-- | |-- | ||
| M1D3 | | M1D3 | ||
| − | | | + | | R/F Feb 16/17 |
| − | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L3 2023.pdf| Lecture slides]] |
| − | | [[20.109( | + | | [[20.109(S23):M1D3 |Assess purity and concentration of purified protein]] <br> [[Media:Sp23 M1D3 nll.pdf| TR Prelab slides]]<br> [[Media:Sp23 M1D3 jz.pdf| WF Prelab slides]] |
| − | | [[20.109( | + | | [[20.109(S23):Homework#Due_M1D3|Homework due]] |
|-- | |-- | ||
| | | | ||
| − | | | + | | T/W Feb 21/22 |
| − | | | + | | |
| − | | <font color = # | + | | <font color = #015526>'''President's Day holiday'''</font color> |
| | | | ||
|-- | |-- | ||
| M1D4 | | M1D4 | ||
| − | | | + | | R/F Feb 23/24 |
| − | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L4 2023.pdf| Lecture slides]] |
| − | | [[20.109( | + | | [[20.109(S23):M1D4 |Confirm purified protein using Western blot]] <br> [[Media:Sp23 M1D4 nll.pdf| TR Prelab slides]]<br> [[Media:Sp23 M1D4 jz.pdf| WF Prelab slides]] |
| − | | <font color= # | + | | <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D4|Homework due]] |
|-- | |-- | ||
| M1D5 | | M1D5 | ||
| − | | | + | | T/W Feb/Mar 28/1 |
| − | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L5 2023.pdf| Lecture slides]] |
| − | | [[20.109( | + | | [[20.109(S23):M1D5 |Image Western blot of purified protein]] <br> [[Media:Sp23 M1D5 nll.pdf| TR Prelab slides]] <br> [[Media:Sp23 M1D5 jz_modded.pdf| WF Prelab slides]] <br> [[Media:Sp23_M1D5_methods_exercise.pdf | Methods Activity]] |
| − | | [[20.109( | + | | [[20.109(S23):Homework#Due_M1D5|Homework due]] <br> |
|-- | |-- | ||
| M1D6 | | M1D6 | ||
| − | | | + | | R/F Mar 2/3 |
| − | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L6 2023.pdf| Lecture slides]] |
| − | | [[20.109( | + | | [[20.109(S23):M1D6 |Prepare and scan small molecule microarray (SMM) slides]] <br> [[Media:Sp23 M1D6 nll.pdf| TR Prelab slides]] <br> [[Media:Sp23 M1D6 jz.pdf| WF Prelab slides]] |
| − | | [[20.109( | + | | [[20.109(S23):Homework#Due_M1D6|Homework due]] <br> [[20.109(S23):Research talk| <font color = #b3251c>'''Research talk due'''</font color>]] Sat, Mar 4 at 10pm <br> |
|-- | |-- | ||
| M1D7 | | M1D7 | ||
| − | | | + | | T/W Mar 7/8 |
| − | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L7 2023.pdf| Lecture slides]] |
| − | | [[20.109( | + | | [[20.109(S23):M1D7 |Analyze SMM data to identify putative small molecule binders]] <br> [[Media:Sp23 M1D7 bcm.pdf | T/R Prelab slides]] <br> [[Media:Sp23 M1D7 jz.pdf | W/F Prelab slides]] |
| − | | | + | | [[20.109(S23):Homework#Due_M1D7|Homework due]] |
|-- | |-- | ||
| − | | | + | | M1D8 |
| − | | | + | | R/F Mar 9/10 |
| − | + | | BE Comm Lab <br> | |
| − | | < | + | | [[20.109(S23):M1D8 |Organize Data summary figures and results]] <br> [[Media:Sp23 M1D8 nll.pdf| TR Prelab slides]] <br> [[Media:Sp23 M1D8 jz.pdf| WF Prelab slides]] |
| − | | [[20.109( | + | | <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D8|Homework due]] |
|-- | |-- | ||
| M2D1 | | M2D1 | ||
| − | | | + | | T/W Mar 14/15 |
| − | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/becky-meyer BCM] <br> [[Media:20.109 Sp23 M2L1.pdf| Lecture slides]] |
| − | | [[20.109( | + | | [[20.109(S23):M2D1 |Determine mutagenesis strategy]] <br> [[Media:Sp23 M2D1 nll.pdf| TR Prelab slides]] <br> [[Media:Sp23 M2D1 jz.pdf| WF Prelab slides]] |
| − | | [[20.109( | + | | [[20.109(S23):Homework#Due_M2D1|Homework due]] |
|-- | |-- | ||
| M2D2 | | M2D2 | ||
| − | | | + | | R/F Mar 16/17 |
| − | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/becky-meyer BCM] <br> [[Media:20.109 Sp23 M2L2.pdf| Lecture slides]] |
| − | | [[20.109( | + | | [[20.109(S23):M2D2 |Perform site-directed mutagenesis]] <br> [[Media:Sp23 M2D2.pdf| TR Prelab slides]] <br> [[Media:Sp23 M2D2.pdf| WF Prelab slides]] |
| − | | [[20.109( | + | | [[20.109(S23):Homework#Due_M2D2|Homework due]] <br> [[20.109(S23):Data Summary| <font color = #b3251c>'''Data Summary draft due'''</font color>]] Sat, Mar 18 at 10 pm <br> [https://app.slack.com/client/T01KU1VLZQB/C04M5JH7XBL Blog post due] Mon, Mar 20 at 10 pm |
|-- | |-- | ||
| M2D3 | | M2D3 | ||
| − | | | + | | T/W Mar 21/22 |
| − | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/becky-meyer BCM] <br> [[Media:20.109 Sp23 M2L3.pdf| Lecture slides]] |
| − | | [[20.109( | + | | [[20.109(S23):M2D3 |Sequence clones and transform into yeast cells]] <br> [[Media:Sp23 M2D3 nll.pdf| TR Prelab slides]] <br> [[Media:Sp23 M2D3 jz.pdf| WF Prelab slides]] |
| − | | [[20.109( | + | | [[20.109(S23):Homework#Due_M2D3|Homework due]] <br> |
|-- | |-- | ||
| M2D4 | | M2D4 | ||
| − | | | + | | R/F Mar 23/24 |
| − | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/becky-meyer BCM] <br> [[Media:20.109 Sp23 M2L4.pdf| Lecture slides]] |
| − | | [[20.109( | + | | [[20.109(S23):M2D4 |Determine transporter mutation and prepare expression experiment]] <br> [[Media:Sp23 M2D4 nll.pdf|TR Prelab slides]] <br> [[Media:Sp23 M2D4 jz.pdf|WF Prelab slides]] |
| − | | <font color= # | + | | <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M2D4|Homework due]] |
|-- | |-- | ||
| − | | | + | | |
| − | | | + | | <font color = 999999>T/W Mar 28/29 - R/F Mar 30/31 </font color> |
| − | + | | | |
| − | | | + | | <font color = #015526>'''Spring Break'''</font color> |
| − | | [[20.109( | + | | [[20.109(S23):Data Summary| <font color = #b3251c>'''Data Summary revision due'''</font color>]] Mon, Mar 27 at 10 pm <br> |
|-- | |-- | ||
| | | | ||
| − | | T/W | + | | T/W Apr 4/5 |
| | | | ||
| − | | [[20.109( | + | | [[20.109(S23):Journal article presentation| <font color = #b3251c>'''Journal article presentations'''</font color>]] |
| | | | ||
|-- | |-- | ||
| | | | ||
| − | | R/F | + | | R/F Apr 6/7 |
| − | | | + | | |
| − | | [[20.109( | + | | [[20.109(S23):Journal article presentation| <font color = #b3251c>'''Journal article presentations'''</font color>]] |
| − | | [https://app.slack.com/client/T01KU1VLZQB/ | + | | [https://app.slack.com/client/T01KU1VLZQB/C04M5JH7XBL Blog post due] Sat, Apr 8 at 10 pm |
| + | |-- | ||
| + | | M2D5 | ||
| + | | T/W Apr 11/12 | ||
| + | | [http://be.mit.edu/directory/becky-meyer BCM] <br> [[Media:20109 Sp23 M2L5.pdf|Lecture slides]] | ||
| + | | [[20.109(S23):M2D5 | Immunofluorescence staining]] <br> [[Media:Sp23 M2D5 bcm.pdf|TR Prelab slides]] <br> [[Media:Sp23 M2D5 jz.pdf|WF Prelab slides]] | ||
| + | | [[20.109(S23):Homework#Due_M2D5|Homework due]] | ||
|-- | |-- | ||
| M2D6 | | M2D6 | ||
| − | | | + | | R/F Apr 13/14 |
| − | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/becky-meyer BCM] <br> [[Media:20.109Sp23 M2L6.pdf|Lecture slides]] |
| − | | [[20.109( | + | | [[20.109(S23):M2D6 | Analyze expression data and prepare metal uptake experiment]] <br> [[Media:Sp23 M2D6 bcm.pdf|TR Prelab slides]] <br> [[Media:Sp23 M2D6 jz.pdf|WF Prelab slides]] |
| − | | [[20.109( | + | | [[20.109(S23):Homework#Due_M2D5|Homework due]] |
|-- | |-- | ||
| | | | ||
| − | | <font color = 999999> | + | | <font color = 999999>T/W Apr 18/19 </font color> |
| | | | ||
| − | | <font color = # | + | | <font color = #015526>'''Patriots' Day holiday'''</font color> |
| | | | ||
|-- | |-- | ||
| M2D7 | | M2D7 | ||
| − | | | + | | R/F Apr 20/21 |
| − | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/becky-meyer BCM] <br> [[Media:20.109 Sp23 M2L7.pdf|Lecture slides]] |
| − | | [[20.109( | + | | [[20.109(S23):M2D7 |Analyze ICP-OES data and examine yeast tolerance to metal]] <br> [[Media:Sp23 M2D7 bcm.pdf|TR Prelab slides]] <br> [[Media:Sp23 M2D7 jz.pdf|WF Prelab slides]] |
| − | | <font color= # | + | | [[20.109(S23):Homework#Due_M2D7|Homework due]] |
| + | |-- | ||
| + | | M2D8 | ||
| + | | T/W Apr 25/26 | ||
| + | | BE Comm Lab <br> | ||
| + | | [[20.109(S23):M2D8 |Complete data analysis and organize Research article figures]] <br> [[Media:Sp23 M2D8 nll.pdf| TR Prelab slides]]<br> [[Media:Sp23 M2D8 jz.pdf| WF Prelab slides]]<br> [[Media:RA discussion bcm.pdf|RA discussion]] | ||
| + | | <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M2D8|Homework due]] | ||
|-- | |-- | ||
| M3D1 | | M3D1 | ||
| − | | R/F | + | | R/F Apr 27/28 |
| − | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/becky-meyer BCM]<br> [[Media:Sp23 Drug discovery ethics exercise.pdf|Discussion slides]] |
| − | | [[20.109( | + | | [[20.109(S23):M3D1 |Brainstorm ideas for Research proposal presentation]] <br> [[Media:Sp23 M3D1 nll.pdf|TR Prelab slides]]<br> [[Media:Sp23 M3D1 jz.pdf|WF Prelab slides]] |
| − | | [[20.109( | + | | [[20.109(S23):Homework#Due_M3D1|Homework due]] <br> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
|-- | |-- | ||
| M3D2 | | M3D2 | ||
| − | | T/W | + | | T/W May 2/3 |
| [http://be.mit.edu/directory/angela-belcher AB]<br> | | [http://be.mit.edu/directory/angela-belcher AB]<br> | ||
| − | | [[20.109( | + | | [[20.109(S23):M3D2 |Pitch research proposal presentation ideas ]] <br> [[Media:Sp23 M3D2 nll.pdf| TR Prelab slides]] <br> [[Media:Sp23 M3D2 jz.pdf| WF Prelab slides]] |
| − | | | + | | [[20.109(S23):Research article| <font color = #b3251c>'''Research article due'''</font color>]] Mon, May 1 at 10 pm <br> [https://app.slack.com/client/T01KU1VLZQB/C04M5JH7XBL Blog post due] Tue, May 2 at 10 pm |
|-- | |-- | ||
| M3D3 | | M3D3 | ||
| − | | R/F | + | | R/F May 4/5 |
| BE Comm Lab | | BE Comm Lab | ||
| − | | [[20.109( | + | | [[20.109(S23):M3D3 |Develop ideas for Research proposal presentation ]] <br> [[Media:Sp23 M3D3 nll.pdf| TR Prelab slides]]<br>[[Media:Sp23 M3D3 nll.pdf| WF Prelab slides]] |
| − | | [[20.109( | + | | [[20.109(S23):Homework#Due_M3D3|Homework due]]<br> |
|-- | |-- | ||
| M3D4 | | M3D4 | ||
| − | | T/W | + | | T/W May 9/10 |
| [http://be.mit.edu/directory/noreen-lyell NLL] <br> | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> | ||
| − | | [[20.109( | + | | [[20.109(S23):M3D4 |Participate in Research proposal peer reviews]] <br> |
| − | | [[20.109( | + | | [[20.109(S23):Homework#Due_M3D4|Homework due]] |
|-- | |-- | ||
| | | | ||
| − | | R/F | + | | R/F May 11/12 |
| | | | ||
| − | | [[20.109( | + | | [[20.109(S23):Research proposal presentation| <font color = #b3251c>'''Research proposal presentations'''</font color>]] |
| − | | [https://app.slack.com/client/T01KU1VLZQB/ | + | | [https://app.slack.com/client/T01KU1VLZQB/C04M5JH7XBL Blog post due] Sat, May 13 at 10 pm |
|-- | |-- | ||
| | | | ||
| − | | T | + | | T May 16 |
| − | | <font color = # | + | | <font color = #015526>'''Celebration lunch!'''</font color> <br> |
| | | | ||
| | | | ||
|} | |} | ||
</div> | </div> | ||
Latest revision as of 16:51, 5 May 2023
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not enough time to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: Tuesday (T) and Thursday (R) 11 - 12 pm in 1-390
Laboratory section times: Tuesday (T) and Thursday (R) 1 - 5 pm or Wednesday (W) and Friday (F) 1 - 5 pm in 56-322
ABSENCE POLICY:
Absences from lecture: Attendance will be recorded for participation points throughout the semester. If absent, student is responsible for all information provided in lecture.
Absences from laboratory: Excused absences should be discussed with the Instructors as soon as possible. Because make-up laboratory time is not provided, attendance in another section may be required to complete the necessary experiments. Unexcused absences will result in a 1/3 of a letter grade deduction from the final grade on the major assignment for the module (for example, a B+ would become a B).
