Difference between revisions of "20.109(S19): Spring 2019 schedule"

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| R/F Mar 21/22
 
| R/F Mar 21/22
 
| [http://be.mit.edu/directory/ernest-fraenkel EF]<br>
 
| [http://be.mit.edu/directory/ernest-fraenkel EF]<br>
| [[20.109(S19):Investigate RNA-seq data using public databases (Day5) | Investigate RNA-seq data using public databases]]<br>
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| [[20.109(S19):Design cell viability experiment and explore additional RNA-seq datasets (Day5) | Design cell viability experiment and explore additional RNA-seq datasets]]<br>
 
| [[20.109(S19):Homework#Due_M2D5| Homework due]]
 
| [[20.109(S19):Homework#Due_M2D5| Homework due]]
 
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| T/W Apr 2/3
 
| T/W Apr 2/3
 
| Comm Lab<br>
 
| Comm Lab<br>
| [[20.109(S19):Journal club presentations (Day6 and 7)| Journal club I presentations]]<br>
+
| [[20.109(S19):Prepare cell viability experiment and complete RNA-seq data analysis| Prepare cell viability experiment and complete RNA-seq data analysis]]<br>
 
|   
 
|   
 
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| R/F Apr 4/5
 
| R/F Apr 4/5
 
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
 
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
| [[20.109(S19):Journal club presentations (Day6 and 7)| Journal club II presentations]]<br>
+
| [[20.109(S19):Journal club presentations (Day7 and 8)| Journal club I presentations]]<br>
 
| [http Blog post due] Sat, Apr 6 at 10 pm <br>
 
| [http Blog post due] Sat, Apr 6 at 10 pm <br>
 
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| T/W Apr 9/10
 
| T/W Apr 9/10
 
| [http://be.mit.edu/directory/ernest-fraenkel EF]<br>
 
| [http://be.mit.edu/directory/ernest-fraenkel EF]<br>
| [[20.109(S19):Induce DNA damage and apply drug treatments for cell viability and identification of regulatory motifs in RNA-seq data (Day8)| Induce DNA damage and apply drug treatments for cell viability and identification of regulatory motifs in RNA-seq data]]<br>
+
| [[20.109(S19):Journal club presentations (Day7 and 8)| Journal club II presentations]]<br>
 
| [[20.109(S19):Homework#Due_M2D8| Homework due]]
 
| [[20.109(S19):Homework#Due_M2D8| Homework due]]
 
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| R/F Apr 11/12
 
| R/F Apr 11/12
 
| [http://be.mit.edu/directory/ernest-fraenkel EF]<br>
 
| [http://be.mit.edu/directory/ernest-fraenkel EF]<br>
| [[20.109(S19):Complete cell viability assay (Day9)| Complete cell viability assay]]<br>
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| [[20.109(S19):Complete data analysis (Day9)| Complete data analysis]]<br>
 
| <font color= #27ae60>'''Laboratory quiz'''</font color> <br> [[20.109(S19):Homework#Due_M2D9| Homework due]] <br>  
 
| <font color= #27ae60>'''Laboratory quiz'''</font color> <br> [[20.109(S19):Homework#Due_M2D9| Homework due]] <br>  
 
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Revision as of 16:25, 31 January 2019

20.109(S19): Laboratory Fundamentals of Biological Engineering

Sp19 banner image v2.png

Spring 2019 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Assessing ligand binding        2. Measuring gene expression        3. Engineering biomaterials              


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm (32-124)
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm (56-322)

MODULE DAY DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
T/W Feb 5/6 NLL
Orientation and laboratory tour
1 1 R/F Feb 7/8 AK
In silico cloning and confirmation digest of protein expression vector
Orientation quiz
Homework due
1 2 T/W Feb 12/13 AK
Purify protein for secondary assays
Homework due
1 3 R/F Feb 14/15 AK
Analyze small molecule microarray (SMM) data
Homework due
T/W Feb 20/21 President's day holiday
1 4 R/F Feb 21/22 AK
Evaluate protein purity and concentration
Laboratory quiz
Homework due
1 5 T/W Feb 26/27 AK
Test protein activity using peptidyl-prolyl cis-trans isomerase assay
Homework due
1 6 R/F Feb/Mar 28/1 AK
Confirm ligand binding using differential scanning fluorimetry assay
Homework due
1 7 T/W Mar 5/6 AK
Complete data analysis
Laboratory quiz
Homework due
2 1 R/F Mar 7/8 NLL
Practice tissue culture techniques and prepare cells for RNA purification
Homework due
2 2 T/W Mar 12/13 NLL
Etoposide treat cells for RNA purification
Homework due
Data summary draft due Mon, Mar 11 at 10 pm
2 3 R/F Mar 14/15 EF
Purify RNA and practice RNA-seq data analysis methods
Homework due
Mini-presentation due Sat, Mar 16 at 10 pm
[http Blog post due] Sun, Mar 17 at 10 pm
2 4 T/W Mar 19/20 EF
Analyze RNA-seq data and prepare for quantitative PCR experiment
Laboratory quiz
Homework due
2 5 R/F Mar 21/22 EF
Design cell viability experiment and explore additional RNA-seq datasets
Homework due
T/W Mar 26/27 Spring vacation Data summary revision due Mon, Mar 25 at 10 pm
R/F Mar 28/29 Spring vacation
2 6 T/W Apr 2/3 Comm Lab
Prepare cell viability experiment and complete RNA-seq data analysis
2 7 R/F Apr 4/5 NLL
Journal club I presentations
[http Blog post due] Sat, Apr 6 at 10 pm
2 8 T/W Apr 9/10 EF
Journal club II presentations
Homework due
2 9 R/F Apr 11/12 EF
Complete data analysis
Laboratory quiz
Homework due
T/W Apr 17/18 Patriots' day holiday
3 1 R/F Apr 18/19 AB
Grow phage-based active (cathode) material
Homework due
Research article due Sat, Apr 20 at 10 pm
[http Blog post due] Sun, Apr 21 at 10 pm
3 2 T/W Apr 23/24 AB
Purify phage-based active (cathode) material
Homework due
3 R/F Apr 25/26 Lecture, but no laboratory
3 3 T/W Apr/May 30/1 AB
Construct cathode with phage-based active material
Laboratory quiz
Homework due
3 4 R/F May 2/3 AB
Visualize phage nanowires using transmission electron microscopy
Homework due
3 5 T/W May 7/8 AB Assemble and test batteries
Laboratory quiz
Homework due
R/F May 9/10 Research proposal presentations [http Blog post due] Sat, May 11 at 10 pm
T May 14 Feedback and celebratory lunch Mini-report due Mon, May 13 at 10 pm