Difference between revisions of "20.109(S17): Spring 2017 schedule"

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| T/W Apr 18/19
 
| T/W Apr 18/19
 
|
 
|
| <font color = #999999>Patriot's day holiday</font color>
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| <font color = #999999>Patriots' day holiday</font color>
 
|
 
|
 
|--
 
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| 1
 
| 1
 
| R/F Apr 20/21  
 
| R/F Apr 20/21  
| [http://be.mit.edu/directory/angela-belcher AB]
+
| [http://be.mit.edu/directory/angela-belcher AB]<br>[[Media:20109_M3L1.pptx.pdf | M3L1]] 
| [[20.109(S17):Growth of phage materials (Day1) | Grow phage active material]]
+
| [[20.109(S17):Growth of phage materials (Day1) | Grow phage active material]]<br>[[Media:Sp17_M3D1_170420_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M3D1_LMM_1.pdf| Prelab W/F]]
 
| [[20.109(S17):Homework#Due_M3D1| Homework due]] <br> [[20.109(S17): Research article| Research article due]] April 22 at 10 pm <br> [http://be20109s17.blogspot.com/ Blog post due] Sun, Apr 23 at 10 pm
 
| [[20.109(S17):Homework#Due_M3D1| Homework due]] <br> [[20.109(S17): Research article| Research article due]] April 22 at 10 pm <br> [http://be20109s17.blogspot.com/ Blog post due] Sun, Apr 23 at 10 pm
 
|--
 
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| 2
 
| 2
 
| T/W Apr 25/26
 
| T/W Apr 25/26
| [http://be.mit.edu/directory/angela-belcher AB]
+
| [http://be.mit.edu/directory/angela-belcher AB]<br>[[Media:20109 M3L2.pptx | M3L2]] 
| [[20.109(S17):Purify active materials (Day2) | Purify phage active material]]
+
| [[20.109(S17):Purify active materials (Day2) | Purify phage active material]]<br>[[Media:Sp17_M3D2_170425_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M3D2_LMM_1.pdf| Prelab W/F]]
 
| <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M3D2| Homework due]]
 
| <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M3D2| Homework due]]
 
|--
 
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| 3
 
| 3
 
| R/F Apr 27/28
 
| R/F Apr 27/28
|
+
|[http://be.mit.edu/directory/angela-belcher AB]<br>
| [[20.109(S17):Cathode construction (Day3)| Construct cathode with phage active material]]
+
| [[20.109(S17):Cathode construction (Day3)| Construct cathode with phage active material]]<br>[[Media:Sp17_M3D3_170427_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M3D3_LMM_1.pdf| Prelab W/F]]
 
| [[20.109(S17):Homework#Due_M3D3| Homework due]]
 
| [[20.109(S17):Homework#Due_M3D3| Homework due]]
 
|--
 
|--
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|  
 
|  
 
| T/W May 2/3
 
| T/W May 2/3
| [http://be.mit.edu/directory/angela-belcher AB]
+
|
 
| <font color = red>Lecture, but no laboratory</font color>
 
| <font color = red>Lecture, but no laboratory</font color>
 
|  
 
|  
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| R/F May 4/5
 
| R/F May 4/5
 
| [http://be.mit.edu/directory/angela-belcher AB]
 
| [http://be.mit.edu/directory/angela-belcher AB]
| [[20.109(S17):TEM (Day4)| Visualize phage nanowires using TEM]]
+
| [[20.109(S17):TEM (Day4)| Visualize phage nanowires using TEM]]<br>[[Media:Sp17_M3D4_170504_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M3D4_LMM_1.pdf| Prelab W/F]]
 
| [[20.109(S17):Homework#Due_M3D4| Homework due]]
 
| [[20.109(S17):Homework#Due_M3D4| Homework due]]
 
|--
 
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| T/W May 9/10
 
| T/W May 9/10
 
| [http://be.mit.edu/directory/angela-belcher AB]
 
| [http://be.mit.edu/directory/angela-belcher AB]
| [[20.109(S17):Battery assembly and testing (Day5) | Assemble and test battery ]]
+
| [[20.109(S17):Battery assembly and testing (Day5) | Assemble and test battery ]]<br>[[Media:Sp17_M3D5_170509_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M3D5_LMM.pdf| Prelab W/F]]
 
| <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M3D5| Homework due]]
 
| <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M3D5| Homework due]]
 
|--
 
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|  
 
|  
 
| R/F May 11/12
 
| R/F May 11/12
|
+
|<font color = red>no lecture</font color>
 
| [[20.109(S17):Research proposal presentation| Research proposal presentations]]
 
| [[20.109(S17):Research proposal presentation| Research proposal presentations]]
 
| [http://be20109s17.blogspot.com/ Blog post due] Thu/Fri, May 11/12 at 10 pm
 
| [http://be20109s17.blogspot.com/ Blog post due] Thu/Fri, May 11/12 at 10 pm

Latest revision as of 19:53, 10 May 2017

20.109(S17): Laboratory Fundamentals of Biological Engineering

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Schedule Spring 2017        Announcements        Assignments        Homework        Communication
       1. High-throughput ligand screening        2. Gene expression engineering        3. Biomaterials engineering              

Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

Schedule details:
Lecture: T/R 11-12 (4-149)
Laboratory sections: T/R 1-5 or W/F 1-5 (56-322)

MODULE DAY DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
T/W Feb 7/8 NLL
Orientation lecture
Orientation
Prelab T/R
Prelab W/F
1 1 R/F Feb 9/10 AK
Snow day
In silico cloning and induce protein expression
Prelab T/R
Prelab W/F
Orientation quiz
Homework due
1 2 T/W Feb 14/15 AK
M1L1
Purify induced protein
Prelab T/R
Prelab W/F
Homework due
1 3 R/F Feb 16/17 AK
M1L2
Evaluate purity and concentration of protein
Prelab T/R
Prelab W/F
Laboratory quiz
Homework due
T/W Feb 21/22 President's day holiday
1 4 R/F Feb 23/24 AK
M1L3
Screen ligand library for FKBP12 binders
Prelab T/R
Prelab W/F
Homework due
1 5 T/W Feb/Mar 28/1 SD
M1L4
Scan slides to identify FKBP12 binders
Prelab T/R
Prelab W/F
Laboratory quiz
Homework due
1 6 R/F Mar 2/3 AK
M1L5
Complete data analysis
Prelab T/R
Prelab W/F
Homework due
1 7 T/W Mar 7/8 AK
M1L6
Identify chemical structures common in FKBP12 binders
Prelab T/R
Prelab W/F
Laboratory quiz
Homework due
2 1 R/F Mar 9/10 LS
M2L1
Confirm cell lines and practice tissue culture techniques
Prelab T/R
Prelab W/F
Homework due
2 2 T/W Mar 14/15 LS
Snow day
Prepare Western blot and induce DNA damage for quantitative PCR assay
Prelab T/R
Prelab W/F
Laboratory quiz
Homework due
Data Summary draft due Mon, Mar 13 at 10 pm
2 3 R/F Mar 16/17 LS
M2L2 & L3
Purify RNA for quantitative PCR assay and treat cells for viability assay
Prelab T/R
Prelab W/F
Homework due
Mini presentation due Sat, Mar 18 at 10 pm
2 4 T/W Mar 21/22 LS
M2L4
Complete cell viability assay and examine transcript levels in response to DNA damage
Prelab T/R
Prelab W/F
Laboratory quiz
Homework due
2 5 R/F Mar 23/24 DC Journal club I
T/W Mar 28/29 Spring vacation Data Summary revision due Mon, Mar 27 at 10 pm
R/F Mar 30/31 Spring vacation
2 6 T/W Apr 4/5 EF
M2L6
Practice data analysis methods
Prelab T/R
Prelab W/F
Blog post due Mon, Apr 3 at 10 pm
Homework due
2 7 R/F Apr 6/7 EF
M2L7
Analyze RNA-seq data
Prelab T/R
Prelab W/F
Homework due
2 8 T/W Apr 11/12 LS
M2L8
Journal club II
2 9 R/F Apr 13/14 NLL
M2L9
Examine quantitative PCR results
Prelab T/R
Prelab W/F
Laboratory quiz
Homework due
Blog post due Sat, Apr 15 at 10 pm
T/W Apr 18/19 Patriots' day holiday
3 1 R/F Apr 20/21 AB
M3L1
Grow phage active material
Prelab T/R
Prelab W/F
Homework due
Research article due April 22 at 10 pm
Blog post due Sun, Apr 23 at 10 pm
3 2 T/W Apr 25/26 AB
M3L2
Purify phage active material
Prelab T/R
Prelab W/F
Laboratory quiz
Homework due
3 3 R/F Apr 27/28 AB
Construct cathode with phage active material
Prelab T/R
Prelab W/F
Homework due
3 T/W May 2/3 Lecture, but no laboratory
3 4 R/F May 4/5 AB Visualize phage nanowires using TEM
Prelab T/R
Prelab W/F
Homework due
3 5 T/W May 9/10 AB Assemble and test battery
Prelab T/R
Prelab W/F
Laboratory quiz
Homework due
R/F May 11/12 no lecture Research proposal presentations Blog post due Thu/Fri, May 11/12 at 10 pm
T/W May 16/17 Open office hours Mini report due Tue/Wed, May 16/17 at 10 pm
R May 18 Feedback and celebratory lunch