Difference between revisions of "20.109(F16): Fall 2016 schedule"
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|'''LABORATORY EXPERIMENTS''' | |'''LABORATORY EXPERIMENTS''' | ||
|'''ASSIGNMENTS''' | |'''ASSIGNMENTS''' | ||
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| [http://be.mit.edu/directory/noreen-lyell NLL]<br> | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | ||
| [[20.109(F16):Lab tour | Orientation]]<br> | | [[20.109(F16):Lab tour | Orientation]]<br> | ||
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| [[20.109(F16):Prepare comet chips (Day1) | Prepare comet chips]]<br> | | [[20.109(F16):Prepare comet chips (Day1) | Prepare comet chips]]<br> | ||
| <font color=#04B45F>'''Lab orientation quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D1| Homework due]] | | <font color=#04B45F>'''Lab orientation quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D1| Homework due]] | ||
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| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
| [[20.109(F16):Test comet chip loading variables (Day2) | Test comet chip loading variables]]<br> | | [[20.109(F16):Test comet chip loading variables (Day2) | Test comet chip loading variables]]<br> | ||
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| [[20.109(F16):Measure DNA damage with comet chip (Day3)| Measure DNA damage with comet chip]] | | [[20.109(F16):Measure DNA damage with comet chip (Day3)| Measure DNA damage with comet chip]] | ||
|<font color=#04B45F>'''Lab quiz'''</font color><br> [[20.109(F16):Homework#Due_M1D3| Homework due]] | |<font color=#04B45F>'''Lab quiz'''</font color><br> [[20.109(F16):Homework#Due_M1D3| Homework due]] | ||
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| <font color = 999999>Career fair student holiday</font color> | | <font color = 999999>Career fair student holiday</font color> | ||
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| [[20.109(F16):Asses DNA repair variability with comet chip (Day4)| Asses DNA repair variability with comet chip]] | | [[20.109(F16):Asses DNA repair variability with comet chip (Day4)| Asses DNA repair variability with comet chip]] | ||
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D4| Homework due]] | | <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D4| Homework due]] | ||
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| [[20.109(F16):Seed cells for immuno-flourescence assay (Day5)| Seed cells for immuno-fluorescence assay]] | | [[20.109(F16):Seed cells for immuno-flourescence assay (Day5)| Seed cells for immuno-fluorescence assay]] | ||
| [[20.109(F16):Homework#Due_M1D5| Homework due]] | | [[20.109(F16):Homework#Due_M1D5| Homework due]] | ||
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| [[20.109(F16):Complete immuno-fluorescence assay (Day6)| Complete immuno-fluorescence assay]] | | [[20.109(F16):Complete immuno-fluorescence assay (Day6)| Complete immuno-fluorescence assay]] | ||
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D6| Homework due]] | | <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D6| Homework due]] | ||
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| [[20.109(F16):Data analysis (Day7)| Data analysis]] | | [[20.109(F16):Data analysis (Day7)| Data analysis]] | ||
| [[20.109(F16):Homework#Due_M1D7| Homework due]] | | [[20.109(F16):Homework#Due_M1D7| Homework due]] | ||
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| <font color = 999999>Columbus day holiday</font color> | | <font color = 999999>Columbus day holiday</font color> | ||
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| [http://be.mit.edu/directory/noreen-lyell NLL] | | [http://be.mit.edu/directory/noreen-lyell NLL] | ||
| [[20.109(F16):Complete in silico cloning (Day1)| Complete in silico cloning]] | | [[20.109(F16):Complete in silico cloning (Day1)| Complete in silico cloning]] | ||
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| [[20.109(F16):Design gRNA for CRISPRi (Day2)| Design gRNA for CRISPRi]] | | [[20.109(F16):Design gRNA for CRISPRi (Day2)| Design gRNA for CRISPRi]] | ||
|<font color=#04B45F>'''Lab quiz'''</font color><br> | |<font color=#04B45F>'''Lab quiz'''</font color><br> | ||
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| [http://be.mit.edu/directory/noreen-lyell NLL] | | [http://be.mit.edu/directory/noreen-lyell NLL] | ||
| [[20.109(F16):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]] | | [[20.109(F16):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]] | ||
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| [http://be.mit.edu/directory/noreen-lyell NLL] | | [http://be.mit.edu/directory/noreen-lyell NLL] | ||
| [[20.109(F16):Journal club I (Day4)| Journal club I]] | | [[20.109(F16):Journal club I (Day4)| Journal club I]] | ||
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| [[20.109(F16):Confirm gRNA sequence (Day5)| Confirm gRNA sequence]] | | [[20.109(F16):Confirm gRNA sequence (Day5)| Confirm gRNA sequence]] | ||
|<font color=#04B45F>'''Lab quiz'''</font color><br> | |<font color=#04B45F>'''Lab quiz'''</font color><br> | ||
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| [http://be.mit.edu/directory/noreen-lyell NLL] | | [http://be.mit.edu/directory/noreen-lyell NLL] | ||
| [[20.109(F16):Journal club II (Day 6) | Journal club II]] | | [[20.109(F16):Journal club II (Day 6) | Journal club II]] | ||
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| [http://be.mit.edu/directory/noreen-lyell NLL] | | [http://be.mit.edu/directory/noreen-lyell NLL] | ||
| [[20.109(F16):Induce CRISPRi system (Day7)| Induce CRISPRi system]] | | [[20.109(F16):Induce CRISPRi system (Day7)| Induce CRISPRi system]] | ||
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| [http://openwetware.org/wiki/20.109(F16):Measure_fermentation_products_(Day8) | Measure fermentation products] | | [http://openwetware.org/wiki/20.109(F16):Measure_fermentation_products_(Day8) | Measure fermentation products] | ||
|<font color=#04B45F>'''Lab quiz'''</font color><br> | |<font color=#04B45F>'''Lab quiz'''</font color><br> | ||
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| <font color = 999999>Veterans' day holiday</font color> | | <font color = 999999>Veterans' day holiday</font color> | ||
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| [[20.109(F16):Growth of phage materials (Day1)| Growth of phage materials]] | | [[20.109(F16):Growth of phage materials (Day1)| Growth of phage materials]] | ||
| [[20.109(F16):Homework#Wrapping_up_M2| Homework due]] <br> [http://openwetware.org/wiki/20.109(F16):_Protein_engineering_report Protein engineering report due] Sun, Nov 15 at 5 pm | | [[20.109(F16):Homework#Wrapping_up_M2| Homework due]] <br> [http://openwetware.org/wiki/20.109(F16):_Protein_engineering_report Protein engineering report due] Sun, Nov 15 at 5 pm | ||
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| [[20.109(F16):Purify active materials (Day2)| Purify active materials]] | | [[20.109(F16):Purify active materials (Day2)| Purify active materials]] | ||
| [[20.109(F16):Homework#Due_M3D2| Homework due]] | | [[20.109(F16):Homework#Due_M3D2| Homework due]] | ||
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| <font color = #FF0000>'''Lecture, but no lab'''</font color> | | <font color = #FF0000>'''Lecture, but no lab'''</font color> | ||
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| <font color = 999999>Thanksgiving holiday</font color> | | <font color = 999999>Thanksgiving holiday</font color> | ||
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| [[20.109(F16):Cathode construction (Day3)| Cathode construction]] | | [[20.109(F16):Cathode construction (Day3)| Cathode construction]] | ||
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M3D3| Homework due]] | | <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M3D3| Homework due]] | ||
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| [[20.109(F16):TEM (Day4)| TEM]] | | [[20.109(F16):TEM (Day4)| TEM]] | ||
| [[20.109(F16):Homework#Due_M3D4| Homework due]] | | [[20.109(F16):Homework#Due_M3D4| Homework due]] | ||
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| [[20.109(F16):Battery assembly and testing (Day5)| Battery assembly and testing]] | | [[20.109(F16):Battery assembly and testing (Day5)| Battery assembly and testing]] | ||
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due (after) M3D5| Homework due]] | | <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due (after) M3D5| Homework due]] | ||
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| [[20.109(F16):Research proposal presentation| Research proposal presentations]] | | [[20.109(F16):Research proposal presentation| Research proposal presentations]] | ||
| [[20.109(F16):Research proposal presentation| Research proposal presentation slides due]] Thu/Fri, Dec 8/9 at 1 pm | | [[20.109(F16):Research proposal presentation| Research proposal presentation slides due]] Thu/Fri, Dec 8/9 at 1 pm | ||
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| colspan="7" | | | colspan="7" | | ||
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| <font color = #00BFFF>'''Feedback and celebratory lunch'''</font color> | | <font color = #00BFFF>'''Feedback and celebratory lunch'''</font color> | ||
| [[20.109(F16): Biomaterials engineering mini-report | Biomaterials engineering mini-report due]] Mon, Dec 12 at 10 pm | | [[20.109(F16): Biomaterials engineering mini-report | Biomaterials engineering mini-report due]] Mon, Dec 12 at 10 pm | ||
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Revision as of 15:02, 31 August 2016
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
Schedule details:
Lecture: T/R 11-12 (16-220)
Laboratory sections: T/R 1-5 or W/F 1-5 (56-322)
| MODULE | DAY | DATE | LECTURER | LABORATORY EXPERIMENTS | ASSIGNMENTS | |
| R/F Sept 8/9 | NLL |
Orientation |
||||
| 1 | 1 | T/W Sept 13/14 | BE | Prepare comet chips |
Lab orientation quiz Homework due | |
| 1 | 2 | R/F Sept 15/16 | BE | Test comet chip loading variables |
||
| 1 | 3 | T/W Sept 20/21 | BE | Measure DNA damage with comet chip | Lab quiz Homework due | |
| R/F Sept 22/23 | Career fair student holiday | |||||
| 1 | 4 | T/W Sept 27/28 | NLL | Asses DNA repair variability with comet chip | Lab quiz Homework due | |
| 1 | 5 | R/F Sept 29/30 | BE | Seed cells for immuno-fluorescence assay | Homework due | |
| 1 | 6 | T/W Oct 4/5 | BE | Complete immuno-fluorescence assay | Lab quiz Homework due | |
| 1 | 7 | R/F Oct 6/7 | BE | Data analysis | Homework due | |
| T/W Oct 11/12 | Columbus day holiday | |||||
| 2 | 1 | R/F Oct 13/14 | NLL | Complete in silico cloning | ||
| 2 | 2 | T/W Oct 18/19 | NLL | Design gRNA for CRISPRi | Lab quiz | |
| 2 | 3 | R/F Oct 20/21 | NLL | Generate gRNA plasmid | ||
| 2 | 4 | T/W Oct 25/26 | NLL | Journal club I | ||
| 2 | 5 | R/F Oct 27/28 | NLL | Confirm gRNA sequence | Lab quiz | |
| 2 | 6 | T/W Nov 1/2 | NLL | Journal club II | ||
| 2 | 7 | R/F Nov 3/4 | NLL | Induce CRISPRi system | ||
| 2 | 8 | T/W Nov 8/9 | NLL | Measure fermentation products] | Lab quiz | |
| R/F Nov 10/11 | Veterans' day holiday | |||||
| 3 | 1 | T/W Nov 15/16 | AB | Growth of phage materials | Homework due Protein engineering report due Sun, Nov 15 at 5 pm | |
| 3 | 2 | R/F Nov 17/18 | AB | Purify active materials | Homework due | |
| T/W Nov 22/23 | AB | Lecture, but no lab | ||||
| R/F Nov 24/25 | Thanksgiving holiday | |||||
| 3 | 3 | T/W Nov 29/30 | AB | Cathode construction | Lab quiz Homework due | |
| 3 | 4 | R/F Dec 1/2 | AB | TEM | Homework due | |
| 3 | 5 | T/W Dec 6/7 | AB | Battery assembly and testing | Lab quiz Homework due | |
| 3 | 6 | R/F Dec 8/9 | Research proposal presentations | Research proposal presentation slides due Thu/Fri, Dec 8/9 at 1 pm | ||
| T Dec 13 | Feedback and celebratory lunch | Biomaterials engineering mini-report due Mon, Dec 12 at 10 pm | ||||
