Difference between revisions of "20.109(F16): Fall 2016 schedule"

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| T/W Sept 13/14  
 
| T/W Sept 13/14  
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
| [[20.109(F16):DNA engineering using PCR (Day1) | DNA engineering using PCR]]<br>
+
| [[20.109(F16):Prepare comet chips (Day1) | Prepare comet chips]]<br>
 
| <font color=#04B45F>'''Lab orientation quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D1| Homework due]]
 
| <font color=#04B45F>'''Lab orientation quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D1| Homework due]]
 
|
 
|
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| R/F Sept 15/16   
 
| R/F Sept 15/16   
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
| [[20.109(F16):Clean and cut DNA (Day2) | Clean and cut DNA]]<br>  
+
| [[20.109(F16):Test comet chip loading variables (Day2) | Test comet chip loading variables]]<br>  
 
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|
 
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| T/W Sept 20/21   
 
| T/W Sept 20/21   
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
| [[20.109(F16):Agarose gel electrophoresis (Day3)| Agarose gel electrophoresis]]
+
| [[20.109(F16):Load comet chips (Day3)| Load comet chips]]
 
| [[20.109(F16):Homework#Due_M1D3| Homework due]]
 
| [[20.109(F16):Homework#Due_M1D3| Homework due]]
 
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| T/W Sept 27/28
 
| T/W Sept 27/28
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
| [[20.109(F16):DNA ligation & transformation (Day4)| Ligation & transformation]]
+
| [[20.109(F16):Measure DNA repair with comet chip (Day4)| Measure DNA repair with comet chip]]
 
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D4| Homework due]]
 
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D4| Homework due]]
 
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| R/F Sept 29/30  
 
| R/F Sept 29/30  
 
| [http://be.mit.edu/directory/noreen-lyell NLL]
 
| [http://be.mit.edu/directory/noreen-lyell NLL]
| [[20.109(F16):Examine candidate clones & tissue culture (Day5)| Examine candidate clones and tissue culture]]
+
| [[20.109(F16):Seed cells for immuno-flourescence assay (Day5)| Seed cells for immuno-fluorescence assay]]
 
| [[20.109(F16):Homework#Due_M1D5| Homework due]]
 
| [[20.109(F16):Homework#Due_M1D5| Homework due]]
 
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| T/W Oct 4/5
 
| T/W Oct 4/5
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
| [[20.109(F16):Lipofection (Day6)| Lipofection]]
+
| [[20.109(F16):Complete immuno-fluorescence assay (Day6)| Complete immuno-fluorescence assay]]
 
| [[20.109(F16):Homework#Due_M1D6| Homework due]]
 
| [[20.109(F16):Homework#Due_M1D6| Homework due]]
 
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| R/F Oct 6/7
 
| R/F Oct 6/7
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
| [[20.109(F16):FACS analysis (Day7)| Data analysis]]
+
| [[20.109(F16):Student business proposals (Day7)| Student business proposals]]
 
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D7| Homework due]]
 
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D7| Homework due]]
 
|
 
|

Revision as of 15:51, 28 June 2016

20.109(F16): Laboratory Fundamentals of Biological Engineering

Engelward PNAS 2006.png

Schedule Fall 2016        Announcements        Assignments        Homework        Communication
       1. Measuring Genomic Instability        2. Manipulating Metabolism        3. Engineering Biomaterials              

Welcome to 20.109! For some of you this will be the first time in a research lab and for others it will not; either way, it is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. There is not time enough to show you everything you’ll need to know if you go on to do research, but after taking this class you should feel confident and familiar with some fundamental experimental approaches and lab protocols. You will develop good habits at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with report writing, notebook keeping, and publicly presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

MODULE DAY DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS DATA / NOTES
R/F Sept 8/9 NLL
Orientation
1 1 T/W Sept 13/14 BE Prepare comet chips
Lab orientation quiz
Homework due
1 2 R/F Sept 15/16 BE Test comet chip loading variables
1 3 T/W Sept 20/21 BE Load comet chips Homework due
R/F Sept 22/23 Career fair student holiday
1 4 T/W Sept 27/28 BE Measure DNA repair with comet chip Lab quiz
Homework due
1 5 R/F Sept 29/30 NLL Seed cells for immuno-fluorescence assay Homework due
1 6 T/W Oct 4/5 BE Complete immuno-fluorescence assay Homework due
1 7 R/F Oct 6/7 BE Student business proposals Lab quiz
Homework due
T/W Oct 11/12 Columbus day holiday
2 1 R/F Oct 13/14 NLL
2 2 T/W Oct 18/19 NLL
2 3 R/F Oct 20/21 NLL
2 4 T/W Oct 25/26 NLL
2 5 R/F Oct 27/28 NLL
2 6 T/W Nov 1/2 NLL
2 7 R/F Nov 3/4 NLL
2 8 T/W Nov 8/9 NLL
R/F Nov 10/11 Veterans' day holiday
3 1 T/W Nov 15/16 AB Growth of phage materials Homework due
Protein engineering report due Sun, Nov 15 at 5 pm‎
3 2 R/F Nov 17/18 AB Purify active materials Homework due
T/W Nov 22/23 AB Lecture, but no lab
R/F Nov 24/25 Thanksgiving holiday
3 3 T/W Nov 29/30 AB Cathode construction Lab quiz
Homework due
3 4 R/F Dec 1/2 AB TEM Homework due
3 5 T/W Dec 6/7 AB Battery assembly and testing Lab quiz
Homework due
3 6 R/F Dec 8/9 Research proposal presentations Research proposal presentation slides due Thu/Fri, Dec 8/9 at 1 pm
T Dec 13 Feedback and celebratory lunch Biomaterials engineering mini-report due Mon, Dec 12 at 10 pm