Difference between revisions of "20.109(S21):Spring 2021 schedule"
Line 36: | Line 36: | ||
| T/W Feb 23/24 | | T/W Feb 23/24 | ||
| [http://be.mit.edu/directory/leslie-mcclain LM] <br> | | [http://be.mit.edu/directory/leslie-mcclain LM] <br> | ||
− | | [[20.109(S21):M1D2 |Enrich candidate clones using FACS]] <br> | + | | [[20.109(S21):M1D2 |Enrich candidate clones from library using FACS]] <br> |
| [[20.109(S21):Homework#Due_M1D2|Homework due]] | | [[20.109(S21):Homework#Due_M1D2|Homework due]] | ||
|-- | |-- |
Revision as of 21:30, 8 January 2021
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times:
- Tuesday (T) and Thursday (R) 11 - 12 pm ([ Zoom access)]
Laboratory section times:
- Tuesday (T) and Thursday (R) 1 - 5 pm ([ Zoom access])
- Wednesday (W) and Friday (F) 1 - 5 pm ([ Zoom access])
MODULE | DATE | LECTURER | LABORATORY EXPERIMENTS | ASSIGNMENTS | |
T/W Feb 16/17 | NLL Slides |
Orientation and laboratory tour |
|||
M1D1 | R/F Feb 18/19 | LM |
In silico cloning library | Orientation quiz Homework due | |
M1D2 | T/W Feb 23/24 | LM |
Enrich candidate clones from library using FACS |
Homework due | |
M1D3 | R/F Feb 25/26 | LM |
Harvest candidate clone plasmids from yeast library |
Homework due | |
M1D4 | T/W Mar 2/3 | LM |
Purify plasmids and sequence clones |
Laboratory quiz Homework due | |
M1D5 | R/F Mar 4/5 | LM |
Analyze clone sequence and choose clone to characterize |
Homework due | |
T/W Mar 9/10 | Student holiday | ||||
M1D6 | R/F Mar 11/12 | LM |
Characterize clone by ligand binding using flow cytometry |
Homework due | |
M1D7 | T/W Mar 16/17 | LM |
Analyze ligand titration curves |
Laboratory quiz Homework due | |
M2D1 | R/F Mar 18/19 | AK |
Placeholder |
Homework due | |
T/W Mar Mar 23/24 | Student holiday | ||||
M2D2 | R/F Mar 25/26 | AK |
Placeholder |
Homework due Data Summary draft due XXX at 10 pm [ Blog post due] XXX at 10 pm | |
M2D3 | T/W Mar 30/31 | AK |
Placeholder |
Homework due | |
M2D4 | R/F Apr 1/2 | AK |
Placeholder |
Laboratory quiz Homework due Mini-presentation due XXX at 10 pm | |
T/W Apr 6/7 | Journal club presentations | ||||
R/F Apr 8/9 | Journal club presentations | [ Blog post due] XXX at 10 pm | |||
M2D5 | T/W Apr 13/14 | AK |
Placeholder | Homework due | |
M2D6 | R/F Apr 15/16 | AK |
Placeholder |
Homework due | |
T/W Apr 20/21 | Student holiday | ||||
M2D7 | R/F Apr 22/23 | AK |
Placeholder |
Laboratory quiz Homework due | |
M3D1 | T/W Apr 27/28 | AJ |
Placeholder |
Homework due | |
M3D2 | R/F Apr 29/30 | AJ |
Placeholder |
Homework due | Research article due XXX at 10 pm [ Blog post due] XXX at 10 pm | |
M3D3 | T/W May 4/5 | AJ |
Placeholder |
Laboratory quiz Homework due | |
R/F May 6/7 | Research proposal pitches |
Develop Research proposal ideas and presentations | |||
M3D4 | T/W May 11/12 | AJ |
Placeholder |
Homework due | |
M3D5 | R/F May 13/14 | AJ |
Placeholder |
Laboratory quiz Homework due | |
T/W May 18/19 | Research proposal presentations | [ Blog post due] XXX at 10 pm | |||
R May 20 | Feedback session |
Mini-report due XXX at 10 pm |