Difference between revisions of "20.109(S17): Spring 2017 schedule"
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| 1 | | 1 | ||
| R/F Feb 9/10 | | R/F Feb 9/10 | ||
− | | [http://be.mit.edu/directory/angela-koehler AK] | + | | [http://be.mit.edu/directory/angela-koehler AK]<br>Snow day |
− | | [[20.109(S17):In silico cloning and induction of protein expression (Day1) |In silico cloning and induce protein expression]] | + | | [[20.109(S17):In silico cloning and induction of protein expression (Day1) |In silico cloning and induce protein expression]]<br>[[Media:SP17_M1D1_170209_marked.pdf| Prelab T/R]]<br>[[Media:M1D1_Sp17_LMM_1.pdf| Prelab W/F]] |
| <font color=#04B45F>'''Orientation quiz'''</font color> <br> [[20.109(S17):Homework#Due_M1D1| Homework due]] | | <font color=#04B45F>'''Orientation quiz'''</font color> <br> [[20.109(S17):Homework#Due_M1D1| Homework due]] | ||
|-- | |-- | ||
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| 2 | | 2 | ||
| T/W Feb 14/15 | | T/W Feb 14/15 | ||
− | | [http://be.mit.edu/directory/angela-koehler AK] | + | | [http://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M1L1.pdf | M1L1]] |
− | | [[20.109(S17):Purification of induced protein (Day2) |Purify induced protein]] | + | | [[20.109(S17):Purification of induced protein (Day2) |Purify induced protein]]<br>[[Media:Sp17_M1D2_170214_marked.pdf| Prelab T/R]]<br>[[Media:SP17_M1D2_LMM_1.pdf| Prelab W/F]]<br> |
| [[20.109(S17):Homework#Due_M1D2| Homework due]] | | [[20.109(S17):Homework#Due_M1D2| Homework due]] | ||
|-- | |-- | ||
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| 3 | | 3 | ||
| R/F Feb 16/17 | | R/F Feb 16/17 | ||
− | | [http://be.mit.edu/directory/angela-koehler AK] | + | | [http://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M1L2.pdf | M1L2]] |
− | | [[20.109(S17):Evaluation of purified protein (Day3) |Evaluate purity and concentration of protein]] | + | | [[20.109(S17):Evaluation of purified protein (Day3) |Evaluate purity and concentration of protein]]<br>[[Media:Sp17_M1D3_170216_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M1D3_LMM_1.pdf| Prelab W/F]] |
| <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M1D3| Homework due]] | | <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M1D3| Homework due]] | ||
|-- | |-- | ||
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| 4 | | 4 | ||
| R/F Feb 23/24 | | R/F Feb 23/24 | ||
− | | [http://be.mit.edu/directory/angela-koehler AK] | + | | [http://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M1L3.pdf | M1L3]] |
− | | [[20.109(S17):Screen chemical library for FKBP12 binders (Day4) |Screen ligand library for FKBP12 binders]] | + | | [[20.109(S17):Screen chemical library for FKBP12 binders (Day4) |Screen ligand library for FKBP12 binders]]<br>[[Media:Sp17_M1D4_170223_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M1D4_LMM_1.pdf| Prelab W/F]] |
| [[20.109(S17):Homework#Due_M1D4| Homework due]] | | [[20.109(S17):Homework#Due_M1D4| Homework due]] | ||
|-- | |-- | ||
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| 5 | | 5 | ||
| T/W Feb/Mar 28/1 | | T/W Feb/Mar 28/1 | ||
− | | [http://koehler-lab.mit.edu/group/people SD] | + | | [http://koehler-lab.mit.edu/group/people SD]<br>[[Media:20109 M1L4.pdf | M1L4]] |
− | | [[20.109(S17):Scan slides to identify FKBP12 binders (Day5) |Scan slides to identify FKBP12 binders]] | + | | [[20.109(S17):Scan slides to identify FKBP12 binders (Day5) |Scan slides to identify FKBP12 binders]]<br>[[Media:Sp17_M1D5_170228_pptx.pdf| Prelab T/R]]<br>[[Media:Sp17_M1D5_LMM_1.pdf| Prelab W/F]] |
| <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M1D5| Homework due]] | | <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M1D5| Homework due]] | ||
|-- | |-- | ||
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| 6 | | 6 | ||
| R/F Mar 2/3 | | R/F Mar 2/3 | ||
− | | [http://be.mit.edu/directory/angela-koehler AK] | + | | [http://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M1L5.pdf | M1L5]] |
− | | [[20.109(S17):Complete data analysis (Day6) |Complete data analysis]] | + | | [[20.109(S17):Complete data analysis (Day6) |Complete data analysis]]<br>[[Media:Prelab M1D6.pdf| Prelab T/R]]<br>[[Media:Prelab M1D6.pdf| Prelab W/F]] |
| [[20.109(S17):Homework#Due_M1D6| Homework due]] | | [[20.109(S17):Homework#Due_M1D6| Homework due]] | ||
|-- | |-- | ||
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| 7 | | 7 | ||
| T/W Mar 7/8 | | T/W Mar 7/8 | ||
− | | [http://be.mit.edu/directory/angela-koehler AK] | + | | [http://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M1L6.pdf | M1L6]] |
− | | [[20.109(S17):Identify chemical structures common among FKBP12 binders |Identify chemical structures common in FKBP12 binders]] | + | | [[20.109(S17):Identify chemical structures common among FKBP12 binders |Identify chemical structures common in FKBP12 binders]]<br>[[Media:Sp17_M1D7_170307_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M1D7_LMM_1.pdf| Prelab W/F]] |
| <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M1D7| Homework due]] | | <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M1D7| Homework due]] | ||
|-- | |-- | ||
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| 1 | | 1 | ||
| R/F Mar 9/10 | | R/F Mar 9/10 | ||
− | | [http://be.mit.edu/directory/leona-d-samson LS] | + | | [http://be.mit.edu/directory/leona-d-samson LS]<br>[[Media:20109 M2L1.pdf | M2L1]] |
− | | [[20.109(S17):Confirm cell lines and practice tissue culture techniques (Day1)| Confirm cell lines and practice tissue culture techniques]] | + | | [[20.109(S17):Confirm cell lines and practice tissue culture techniques (Day1)| Confirm cell lines and practice tissue culture techniques]]<br>[[Media:Sp17_M2D1_170309_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M2D1_LMM_1.pdf| Prelab W/F]] |
| [[20.109(S17):Homework#Due_M2D1| Homework due]] | | [[20.109(S17):Homework#Due_M2D1| Homework due]] | ||
|-- | |-- | ||
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| 2 | | 2 | ||
| T/W Mar 14/15 | | T/W Mar 14/15 | ||
− | | [http://be.mit.edu/directory/leona-d-samson LS] | + | | [http://be.mit.edu/directory/leona-d-samson LS]<br>Snow day |
− | | [[20.109(S17): | + | | [[20.109(S17):Prepare Western blot and induce DNA damage for quantitative PCR assay (Day2)| Prepare Western blot and induce DNA damage for quantitative PCR assay]]<br>[[Media:Sp17_M2D2_170314_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M2D2_LMM_1.pdf| Prelab W/F]] |
| <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M2D2| Homework due]]<br> [[20.109(S17): M1 Data Summary| Data Summary draft due]] Mon, Mar 13 at 10 pm | | <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M2D2| Homework due]]<br> [[20.109(S17): M1 Data Summary| Data Summary draft due]] Mon, Mar 13 at 10 pm | ||
|-- | |-- | ||
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| 3 | | 3 | ||
| R/F Mar 16/17 | | R/F Mar 16/17 | ||
− | | [http://be.mit.edu/directory/leona-d-samson LS] | + | | [http://be.mit.edu/directory/leona-d-samson LS] <br> [[Media:FINAL 20.109 Spring 2017 Lectures 2 and 3 (snow day).pdf| M2L2 & L3]] |
− | | [[20.109(S17): | + | | [[20.109(S17):Purify RNA for quantitative PCR assay and treat cells for survival assay (Day3)| Purify RNA for quantitative PCR assay and treat cells for viability assay]]<br>[[Media:Sp17_M2D3_170316_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M2D3_LMM_1.pdf| Prelab W/F]] |
| [[20.109(S17):Homework#Due_M2D3| Homework due]] <br> [[20.109(S17): M1 Mini-presentation| Mini presentation due]] Sat, Mar 18 at 10 pm | | [[20.109(S17):Homework#Due_M2D3| Homework due]] <br> [[20.109(S17): M1 Mini-presentation| Mini presentation due]] Sat, Mar 18 at 10 pm | ||
|-- | |-- | ||
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| 4 | | 4 | ||
| T/W Mar 21/22 | | T/W Mar 21/22 | ||
− | | [http://be.mit.edu/directory/leona-d-samson LS] | + | | [http://be.mit.edu/directory/leona-d-samson LS]<br> [[Media:Sp17 M2L4.pdf| M2L4]] |
− | | [[20.109(S17): | + | | [[20.109(S17):Complete cell survival assay and examine transcript levels in response to DNA damage (Day 4)| Complete cell viability assay and examine transcript levels in response to DNA damage]]<br>[[Media:Sp17_M2D4_170320_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M2D4_LMM_1.pdf| Prelab W/F]] |
| <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M2D4| Homework due]] | | <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M2D4| Homework due]] | ||
|-- | |-- | ||
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| 5 | | 5 | ||
| R/F Mar 23/24 | | R/F Mar 23/24 | ||
− | | | + | | DC |
| [[20.109(S17):Journal Club I (Day5) | Journal club I]] | | [[20.109(S17):Journal Club I (Day5) | Journal club I]] | ||
| | | | ||
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| 6 | | 6 | ||
| T/W Apr 4/5 | | T/W Apr 4/5 | ||
− | | [http://be.mit.edu/directory/ernest-fraenkel EF] | + | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br>[[Media:170404 Fraenkel Lecture 1.pdf| M2L6]] |
− | | [[20.109(S17):Practice data analysis methods (Day6)| Practice data analysis methods]] | + | | [[20.109(S17):Practice data analysis methods (Day6)| Practice data analysis methods]]<br>[[Media:Sp17_M2D6_170404_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M2D6_LMM_1.pdf| Prelab W/F]] |
| [http://be20109s17.blogspot.com/ Blog post due] Mon, Apr 3 at 10 pm <br> [[20.109(S17):Homework#Due_M2D6| Homework due]] | | [http://be20109s17.blogspot.com/ Blog post due] Mon, Apr 3 at 10 pm <br> [[20.109(S17):Homework#Due_M2D6| Homework due]] | ||
|-- | |-- | ||
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| 7 | | 7 | ||
| R/F Apr 6/7 | | R/F Apr 6/7 | ||
− | | [http://be.mit.edu/directory/ernest-fraenkel EF] | + | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br>[[Media:170406 Fraenkel Lecture 2.pdf| M2L7]] |
− | | [[20.109(S17):Analyze RNA-seq data (Day7) | Analyze RNA-seq data]] | + | | [[20.109(S17):Analyze RNA-seq data (Day7) | Analyze RNA-seq data]]<br>[[Media:Sp17_M2D7_170406_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M2D7_LMM_1.pdf| Prelab W/F]] |
| [[20.109(S17):Homework#Due_M2D7| Homework due]] | | [[20.109(S17):Homework#Due_M2D7| Homework due]] | ||
|-- | |-- | ||
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| 8 | | 8 | ||
| T/W Apr 11/12 | | T/W Apr 11/12 | ||
− | | [http://be.mit.edu/directory/leona-d-samson LS] | + | | [http://be.mit.edu/directory/leona-d-samson LS] <br> [[Media:THE FINAL 20.109 Spring 2017 Lecture 5.pptx| M2L8]] |
| [[20.109(S17):Journal Club II (Day8)| Journal club II]] | | [[20.109(S17):Journal Club II (Day8)| Journal club II]] | ||
| | | | ||
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| 9 | | 9 | ||
| R/F Apr 13/14 | | R/F Apr 13/14 | ||
− | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Sp17 M2D9 lecture slides.pdf| M2L9]] |
− | | [[20.109(S17):Examine quantitative PCR results (Day9)| Examine quantitative PCR results]] | + | | [[20.109(S17):Examine quantitative PCR results (Day9)| Examine quantitative PCR results]]<br>[[Media:Sp17_M2D9_170413_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M2D9_LMM_1.pdf| Prelab W/F]] |
| <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M2D9| Homework due]] <br> [http://be20109s17.blogspot.com/ Blog post due] Sat, Apr 15 at 10 pm | | <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M2D9| Homework due]] <br> [http://be20109s17.blogspot.com/ Blog post due] Sat, Apr 15 at 10 pm | ||
|-- | |-- | ||
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| T/W Apr 18/19 | | T/W Apr 18/19 | ||
| | | | ||
− | | <font color = #999999> | + | | <font color = #999999>Patriots' day holiday</font color> |
| | | | ||
|-- | |-- | ||
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| 1 | | 1 | ||
| R/F Apr 20/21 | | R/F Apr 20/21 | ||
− | | [http://be.mit.edu/directory/angela-belcher AB] | + | | [http://be.mit.edu/directory/angela-belcher AB]<br>[[Media:20109_M3L1.pptx.pdf | M3L1]] |
− | | [[20.109(S17):Growth of phage materials (Day1) | Grow phage active material]] | + | | [[20.109(S17):Growth of phage materials (Day1) | Grow phage active material]]<br>[[Media:Sp17_M3D1_170420_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M3D1_LMM_1.pdf| Prelab W/F]] |
| [[20.109(S17):Homework#Due_M3D1| Homework due]] <br> [[20.109(S17): Research article| Research article due]] April 22 at 10 pm <br> [http://be20109s17.blogspot.com/ Blog post due] Sun, Apr 23 at 10 pm | | [[20.109(S17):Homework#Due_M3D1| Homework due]] <br> [[20.109(S17): Research article| Research article due]] April 22 at 10 pm <br> [http://be20109s17.blogspot.com/ Blog post due] Sun, Apr 23 at 10 pm | ||
|-- | |-- | ||
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| 2 | | 2 | ||
| T/W Apr 25/26 | | T/W Apr 25/26 | ||
− | | [http://be.mit.edu/directory/angela-belcher AB] | + | | [http://be.mit.edu/directory/angela-belcher AB]<br>[[Media:20109 M3L2.pptx | M3L2]] |
− | | [[20.109(S17):Purify active materials (Day2) | Purify phage active material]] | + | | [[20.109(S17):Purify active materials (Day2) | Purify phage active material]]<br>[[Media:Sp17_M3D2_170425_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M3D2_LMM_1.pdf| Prelab W/F]] |
| <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M3D2| Homework due]] | | <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M3D2| Homework due]] | ||
|-- | |-- | ||
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| 3 | | 3 | ||
| R/F Apr 27/28 | | R/F Apr 27/28 | ||
− | | | + | |[http://be.mit.edu/directory/angela-belcher AB]<br> |
− | | [[20.109(S17):Cathode construction (Day3)| Construct cathode with phage active material]] | + | | [[20.109(S17):Cathode construction (Day3)| Construct cathode with phage active material]]<br>[[Media:Sp17_M3D3_170427_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M3D3_LMM_1.pdf| Prelab W/F]] |
| [[20.109(S17):Homework#Due_M3D3| Homework due]] | | [[20.109(S17):Homework#Due_M3D3| Homework due]] | ||
|-- | |-- | ||
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| | | | ||
| T/W May 2/3 | | T/W May 2/3 | ||
− | | | + | | |
| <font color = red>Lecture, but no laboratory</font color> | | <font color = red>Lecture, but no laboratory</font color> | ||
| | | | ||
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| R/F May 4/5 | | R/F May 4/5 | ||
| [http://be.mit.edu/directory/angela-belcher AB] | | [http://be.mit.edu/directory/angela-belcher AB] | ||
− | | [[20.109(S17):TEM (Day4)| Visualize phage nanowires using TEM]] | + | | [[20.109(S17):TEM (Day4)| Visualize phage nanowires using TEM]]<br>[[Media:Sp17_M3D4_170504_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M3D4_LMM_1.pdf| Prelab W/F]] |
| [[20.109(S17):Homework#Due_M3D4| Homework due]] | | [[20.109(S17):Homework#Due_M3D4| Homework due]] | ||
|-- | |-- | ||
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| T/W May 9/10 | | T/W May 9/10 | ||
| [http://be.mit.edu/directory/angela-belcher AB] | | [http://be.mit.edu/directory/angela-belcher AB] | ||
− | | [[20.109(S17):Battery assembly and testing (Day5) | Assemble and test battery ]] | + | | [[20.109(S17):Battery assembly and testing (Day5) | Assemble and test battery ]]<br>[[Media:Sp17_M3D5_170509_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M3D5_LMM.pdf| Prelab W/F]] |
| <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M3D5| Homework due]] | | <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M3D5| Homework due]] | ||
|-- | |-- | ||
Line 214: | Line 214: | ||
| | | | ||
| R/F May 11/12 | | R/F May 11/12 | ||
− | | | + | |<font color = red>no lecture</font color> |
| [[20.109(S17):Research proposal presentation| Research proposal presentations]] | | [[20.109(S17):Research proposal presentation| Research proposal presentations]] | ||
| [http://be20109s17.blogspot.com/ Blog post due] Thu/Fri, May 11/12 at 10 pm | | [http://be20109s17.blogspot.com/ Blog post due] Thu/Fri, May 11/12 at 10 pm |
Latest revision as of 19:53, 10 May 2017
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
Schedule details:
Lecture: T/R 11-12 (4-149)
Laboratory sections: T/R 1-5 or W/F 1-5 (56-322)