Difference between revisions of "DNA Melting Report Requirements for Part 1"
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#Plot fluorescence versus time for one minute and estimate the noise level by calculating the variance. | #Plot fluorescence versus time for one minute and estimate the noise level by calculating the variance. | ||
#Using the same amplifier gain, estimate the change in signal between DNA sample in and sample out. | #Using the same amplifier gain, estimate the change in signal between DNA sample in and sample out. | ||
− | ##What is the signal to noise ratio (SNR) in units of dB for your system? | + | ##What is the signal to noise ratio (SNR) in units of dB for your system? (Ensure that your system has an SNR of at least 12 dB.) |
− | + | ||
#Obtain a DNA melting curve by plotting fluorescence versus temperature. | #Obtain a DNA melting curve by plotting fluorescence versus temperature. | ||
##Repeat two more times. | ##Repeat two more times. |
Revision as of 22:55, 23 October 2012
Report requirements
- Report submittal format
- Size of your lab report file submitted MUST be less than 20 MB
- Post only pdf, with code included in the pdf at the end, not separately
- Include last name of each group member in the filename of the pdf you post. I would rather spend time carefully grading your report than renaming your files because every other group named theirs "DNA Report Part 1" or the like.
- Plot fluorescence versus time for one minute and estimate the noise level by calculating the variance.
- Using the same amplifier gain, estimate the change in signal between DNA sample in and sample out.
- What is the signal to noise ratio (SNR) in units of dB for your system? (Ensure that your system has an SNR of at least 12 dB.)
- Obtain a DNA melting curve by plotting fluorescence versus temperature.
- Repeat two more times.
- On one graph, plot your three melting curves.
- Pick your favorite of the three melting curves and use your code from the simulation to perform a least squares fit and comment on the results.
- What values do you obtain for ΔH and ΔS? Do you trust these values?
Lab manual sections
- DNA Melting: Simulating DNA Melting - Basics
- DNA Melting Part 1: Measuring Temperature and Fluorescence
- DNA Melting Report Requirements for Part 1
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA Melting Part 2: Lock-in Amplifier and Temperature Control
- DNA Melting Report Requirements for Part 2