DNA Melting Part 2: Lock-in Amplifier and Temperature Control
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This page is being revised. To be finalized by November 6th, 2011 -Steven |
Overview of Part 2
Hardware changes
Possibly adjust your optics based on results in Part 1
Add LED driver
Add heater control board
Make new connections to DAQ
Add high-pass filter and possibly adjust gains in your amplifier
Software changes
Inserted lock-in amplifier in Labview VI
Inserted temperature controller in Labview VI
(Talk about possibility of using Arduino)
Why use a lock-in amplifier?
Fundamentals
Show simulation of noise sources
Reference lock-in demo
Update your electrical system
LED driver
The LED used in this lab can consume 1.5 Amps of current when driven to its full range, but this is more than 10x the light you will need. In fact, you will need only roughly 100 mA, but even this low current is 50x what the analog voltage output sources in the DAQ systems are capable of driving[1][2]. And even if there were enough current available, the steepness of a diode's I-V curve results in large swings in current for small changes in supply voltage. LED radiant flux is proportional to current, so a current source tends to provide a more stable light output.
To implement a current source to drive your LED, you will be using a Darlington Transistor [3]. This is a special device in which one transistor drives another to provide higher input-output current gain. At the LED current levels for your instrument, the TIP120 will be providing a current gain of about 500. A transistor [4] is a three terminal device in which the voltage or current at one terminal controls the voltage across, or current through, the other two terminals. In our case, the TIP120 acts like one BJT consisting of a Base, Emitter, and Collector, and should be wired into the circuit shown in the diagram. We will use the base contact of our TIP120 to accept a small amount of current from an op-amp, which will cause the TIP120 to conduct a much larger current between it's collector and emitter, in turn allowing nearly the same level of current to flow through the LED.
As described in lecture, the op-amp will be used to linearize the behavior of the TIP120 and the LED. Here the LED and transistor are inside a feedback loop extending from the output of the op-amp, through the base-emitter conduction path in the TIP120, through the LED, and back from the top of the 1 Ohm resistor, whose voltage drop is thus fed-back to the negative input node of the op-amp, completing the loop. Recall that this is simply a buffer implementation, with gain of 1. Here the feedback voltage is directly proportional to the LED current. Notice that in this way the current through the LED is exactly equal to the voltage applied to the op-amp positive input by the DAQ. (Remember the golden rules.)
Note: if the LED is too bright then the sample will become photobleached very quickly. In the Labview VI for Part 2, you are able to set the voltage supplied to the op-amp.
Note also that the voltage to the LED stil comes from the fixed 5V supply on your lab station's INSTEK GPS-3303 power supply, and the op-amps still operate on +/– VCC (+/-15V in your setup).
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As before, double check your wiring before powering the LED. The LED can be damaged by excessive current. Reduce the offset and amplitude of your driving current to protect the LED as well as to minimize photobleaching. |
Amplifier gain adjustments and high-pass
Add heater controller board
Update your data acquisition system
New inputs to heater controller and LED controller
Use lock-in version of Labview VI
Part 2 Experimental procedure
Groups of different sample types: 3 knowns, one unknown of from that set
Measure SNR
Lab manual sections
- DNA Melting: Simulating DNA Melting - Basics
- DNA Melting Part 1: Measuring Temperature and Fluorescence
- DNA Melting Report Requirements for Part 1
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA Melting Part 2: Lock-in Amplifier and Temperature Control
- DNA Melting Report Requirements for Part 2
References
- ↑ Datasheet for the USB-6212
- ↑ Datasheet for the USB-6341
- ↑ Darlington Transistor
- ↑ Using a transistor
