20.109(S21): Laboratory Fundamentals of Biological Engineering
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times:
- Tuesday (T) and Thursday (R) 11 - 12 pm ([ Zoom access)]
Laboratory section times:
- Tuesday (T) and Thursday (R) 1 - 5 pm ([ Zoom access])
- Wednesday (W) and Friday (F) 1 - 5 pm ([ Zoom access])
MODULE
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DATE
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LECTURER
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LABORATORY EXPERIMENTS
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ASSIGNMENTS
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T/W Feb 16/17
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NLL Slides
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Orientation and laboratory tour
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M1D1
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R/F Feb 18/19
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LM
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Placeholder
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Orientation quiz Homework due
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M1D2
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T/W Feb 23/24
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LM
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Placeholder
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Homework due
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M1D3
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R/F Feb 25/26
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LM
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Placeholder
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Homework due
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M1D4
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T/W Mar 2/3
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LM
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Placeholder
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Laboratory quiz Homework due
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M1D5
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R/F Mar 4/5
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LM
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Placeholder
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Homework due
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T/W Mar 9/10
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Student holiday
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M1D6
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R/F Mar 11/12
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LM
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Placeholder
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Homework due
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M1D7
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T/W Mar 16/17
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LM
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Placeholder
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Laboratory quiz Homework due
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M2D1
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R/F Mar 18/19
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AK
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Placeholder
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Homework due
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M2D2
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R/F Oct 1/2
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AK
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Placeholder
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Homework due Data Summary draft due XXX at 10 pm [ Blog post due] XXX at 10 pm
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M2D3
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T/W Oct 6/7
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AK
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Assess purity and concentration of purified protein T/R Zoom access, T/R prelab video, T/R prelab slides purification notes W/F Zoom access, W/F prelab video, W/F prelab slides
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Homework due
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M2D4
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R/F Oct 8/9
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JN Zoom access Video Slides
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Prepare small molecule microarray (SMM) slides with purified protein T/R Zoom access, T/R prelab video, T/R prelab slides W/F Zoom access, W/F prelab video, W/F prelab slides
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Laboratory quiz Homework due Mini-presentation due Sun, Oct 11 at 10 pm
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T/W Oct 13/14
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Indigenous Peoples Day holiday
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Data Summary revision due Wed, Oct 14 at 10 pm
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M2D5
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R/F Oct 15/16
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JN Zoom access Video Slides
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Scan SMM slides to identify putative small molecule binders T/R Zoom access, T/R prelab video, T/R prelab slides W/F Zoom access, W/F prelab video, W/F prelab slides
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Homework due
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T/W Oct 20/21
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Journal club presentations
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R/F Oct 22/23
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Journal club presentations
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Blog post due Sun, Oct 25 at 10 pm
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M2D6
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T/W Oct 27/28
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JN Zoom access Video Slides
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Analyze SMM data to confirm putative small molecule binders T/R Zoom access, T/R prelab video, T/R prelab slides W/F Zoom access, W/F prelab video, W/F prelab slides
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Homework due
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M2D7
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R/F Oct 29/30
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JN Zoom access Video Slides
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Examine putative small molecule binders for common features T/R Zoom access, T/R prelab video, T/R prelab slides W/F Zoom access, W/F prelab video, W/F prelab slides
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Laboratory quiz Homework due
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M3D1
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T/W Nov 3/4
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NLL Zoom access Video Slides
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Research project scope and experimental strategy T/R Zoom access, T/R prelab video, T/R prelab slides W/F Zoom access, W/F prelab video, W/F prelab slides
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Homework due
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M3D2
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R/F Nov 5/6
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NLL Zoom access Video Slides
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Align gRNA sequences with genomic targets T/R Zoom access, T/R prelab video, T/R prelab slides W/F Zoom access, W/F prelab video, W/F prelab slides
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Homework due
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T/W Nov 10/11
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Veterans day holiday
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Research article due Wed, Nov 11 at 10 pm Blog post due Thu, Nov 12 at 10 pm
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M3D3
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R/F Nov 12/13
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NLL Zoom access Video Slides
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Analyze ethanol yield data T/R Zoom access, [ T/R prelab video], T/R prelab slides W/F Zoom access, [ W/F prelab video], W/F prelab slides
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Laboratory quiz Homework due
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T/W Nov 17/18
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Research proposal pitches Zoom access
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Develop Research proposal ideas and presentations T/R Zoom access W/F Zoom access
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M3D4
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R/F Nov 19/20
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NLL Zoom access [ Video] Slides
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Examine features in gRNA-targeted genomic sequences T/R Zoom access, [ T/R prelab video], T/R prelab slides W/F Zoom access, [ W/F prelab video], W/F prelab slides
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Homework due
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T/W Nov 24/25
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Thanksgiving holiday
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R/F Nov 26/27
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Thanksgiving holiday
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M3D5
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T/W Dec 1/2
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NLL Zoom access [ Video] Slides
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Design optimized gRNA sequence for enhanced ethanol production T/R Zoom access, [ T/R prelab video], T/R prelab slides W/F Zoom access, [ W/F prelab video], W/F prelab slides
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Laboratory quiz Homework due
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R/F Dec 3/F
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Research proposal presentations
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Blog post due Fri, Dec 4 at 10 pm
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T Dec 8
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Feedback session Zoom access
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Mini-report due Mon, Dec 7 at 10 pm
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