Difference between revisions of "DNA Melting Report Requirements for Part 1"
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==Report requirements== | ==Report requirements== | ||
− | + | * One member of your group should submit a single PDF file to Stellar in advance of the deadline. The filename should consist of the last names of all group members, CamelCased, in alphabetical order, with a .pdf extension. Example: <code>CrickFranklinWatson.pdf</code>. | |
− | + | * The file must be less than 20 MB. | |
− | + | * Include code at the end of the document in an appendix. | |
− | + | ||
− | # | + | ==Part 1 report outline== |
− | #Plot | + | # Document your circuit design, optical design, and any changes you made to the system described in the lab manual. |
− | # | + | # Plot at least one melting curve. |
− | #* | + | #* The plot should have temperature in °C on the horizontal axis and fraction of double stranded DNA on the vertical axis. |
− | # | + | #* On the same set of axes, include a simulated curve generated by DINAMelt, OligoCalc, or another software simulator. |
+ | #* Also on the same set of axes, plot the output of the <tt>DnaFraction</tt> function evaluated with best-fit parameters. | ||
+ | #* Include a legend. | ||
+ | # Report the estimated melting temperature and the best-fit values of ''ΔH°'', ''ΔS°''. | ||
+ | # Report your signal to noise measurement. | ||
==Lab manual sections== | ==Lab manual sections== |
Revision as of 11:00, 23 April 2015
Report requirements
- One member of your group should submit a single PDF file to Stellar in advance of the deadline. The filename should consist of the last names of all group members, CamelCased, in alphabetical order, with a .pdf extension. Example:
CrickFranklinWatson.pdf
. - The file must be less than 20 MB.
- Include code at the end of the document in an appendix.
Part 1 report outline
- Document your circuit design, optical design, and any changes you made to the system described in the lab manual.
- Plot at least one melting curve.
- The plot should have temperature in °C on the horizontal axis and fraction of double stranded DNA on the vertical axis.
- On the same set of axes, include a simulated curve generated by DINAMelt, OligoCalc, or another software simulator.
- Also on the same set of axes, plot the output of the DnaFraction function evaluated with best-fit parameters.
- Include a legend.
- Report the estimated melting temperature and the best-fit values of ΔH°, ΔS°.
- Report your signal to noise measurement.