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• 20.345:Course Information
  ...ity to pursue a specific technique in depth. You will have many chances to work on your writing, presentation, and project planning skills. Active particip ...Developing an entirely new in vitro neuronal guidance technology is folly. Work closely with your mentor to find the right balance. The lab has a modest bu
  11 KB (1,722 words) - 17:22, 6 February 2018
• 20.345:Final project proposal
  ...uld detail what you are planning to do, the motivation for doing it, prior work, and relevant theory. It should include a comprehensive development plan an ...m line on motivation, goal, key milestones, risks, resources, and previous work.
  2 KB (283 words) - 00:40, 12 February 2014
• Spring 2011:CellFinder
  *we have little electronics experience, and Jenn needs an electronics review :) ****this didn't work either because the polymer is too hydrophobic or it is blocked
  4 KB (610 words) - 00:07, 3 April 2011
• Spring 11:QRT-PCR
  ...ize the current needed to heat the wire, while still being thick enough to work with. The thermal epoxy ensures that the resistance wire does not make cont We definitely see less smearing with newer version. There is of course much work still to do.
  8 KB (1,345 words) - 19:36, 19 May 2011
• Spring 11:QRT-PCR weekly reports
  * ordered parts for heated lid may not work, or may not come in for a while * PCR may not work even with heated lid - will need to think of other ways to fix this problem
  3 KB (447 words) - 04:09, 8 April 2011
• Weekly Updates
  - Work will start with the image analysis programming, including the analysis of c ...on seems to be adapting the QuickPALM code available in open source to our needs
  9 KB (1,454 words) - 20:19, 19 May 2011
• Spring 11:Chemotaxis Assay
  * needs Power of .5W (12V-DC, 0.04 Amps) ... precise control of the fluid (chemoeffector band width and stoppped flow) needs to be improved.
  14 KB (2,196 words) - 23:14, 18 May 2011
• DNA Melting: Simulating DNA Melting - Basics
  If you prefer to work in Python, please see [[Python:Simulating DNA Melting|this page]]. Does the <tt>DnaFraction</tt> function work as expected? A good way to test the function is to plot a melting curve gen
  13 KB (2,026 words) - 22:46, 15 April 2018
• Spring 11:CellPrinter
  Work performed and risks identified along the way.
  519 B (60 words) - 19:38, 19 May 2011
• March14
  ===Work Performed===
  125 B (15 words) - 16:51, 30 March 2011
• QRT-PCR:Heated Lid
  ...nd silicone tape sandwiched between two plates of aluminum...but it didn't work too well. They said it "smoked and burned out." However, they were designin
  3 KB (571 words) - 19:07, 4 April 2011
• Spring 11:Directed Evolution/Week of 4-04-2011 Status
  ...hernet Controller]. We now have control via RS232 (couldn't get TCP/IP to work)
  925 B (132 words) - 20:52, 15 May 2011
• QRT-PCR:Get Started
  This page is designed to be useful for whoever is just starting to work on the 20.309/345 QRT-PCR machine.
  901 B (144 words) - 23:03, 4 May 2011
• Spring 11:Directed Evolution/Week of 5-09-2011 Status
  Also attempted to pour some agar plugs which didn't work out very well.
  792 B (113 words) - 03:32, 15 May 2011
• Weekly Progress Reports
  ===Work=== ===Work===
  5 KB (849 words) - 08:32, 19 May 2011
• Spring 11:QRT-PCR: Progress Notes
  ...roke. The lid is not hooked up, but it is ready. I wish I had been able to work faster and do more, but... I'll be back tomorrow. ...iew code. I will be working with versions 2.700 and later. Not all of them work. I will take notes on this; take a look at [[QRT-PCR:LabView-versions]].
  7 KB (1,228 words) - 16:48, 19 May 2011
• Confocal Wiki
  ... confocal microscope in the 20.309 laboratory. Stephanie Bachar continued work on the microscope during January of 2011. When beginning our project, the
  21 KB (3,664 words) - 21:38, 4 June 2015
• Theory
  ... the camera in each photoswitching cycle. This calculation is based on the work of Thompson et al. where a detailed analysis of single molecule localizatio
  9 KB (1,442 words) - 20:08, 19 May 2011
• Cartridge Manipulation
  ... After tapping the screwdriver into the groove, the cover would gradually work its way off, though cracks would occur in the plastic around the top edge.
  7 KB (1,060 words) - 13:29, 19 May 2011
• Photobleaching Model
  ...er for us to use? (Kaduk says that a nonpolar polymer gel-like thing might work a lot better than trying to find glycerol-plus.) Of course, diffusion is ab A thought about the glycerol thing -- why doesn't this work with gels? Or does it? Viscosity is not the same as gelification, I suppose
  11 KB (1,755 words) - 23:31, 18 July 2011