Difference between revisions of "Assignment 3 Overview"
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{{Template:Assignment Turn In|message= Here is a comprehensive list of what you need to turn in: | {{Template:Assignment Turn In|message= Here is a comprehensive list of what you need to turn in: | ||
# A figure with images of the stained cell samples with and without Cyto-D. | # A figure with images of the stained cell samples with and without Cyto-D. | ||
− | #* For each sample, create 1 figure with | + | #* For each sample, create 1 figure with 6 panels. |
− | #* The panels of the figure should be: A) unprocessed image; B) | + | #* The panels of the figure should be: A) unprocessed image; B) dark image; C) reference image; D) dark reference image; E) flat-field corrected image; and F) histogram. |
#* In the caption, specify the exposure and gain settings. Each image should have a scale bar. State the dimension of the scale bar in the caption. | #* In the caption, specify the exposure and gain settings. Each image should have a scale bar. State the dimension of the scale bar in the caption. | ||
#* For panel E, plot histograms of the unprocessed, dark, reference, and corrected image on the same set of axes. Plot <tt>log10( count )</tt> on the vertical axis and intensity on the horizontal axis. Use a line plot instead of a bar chart for the histogram. | #* For panel E, plot histograms of the unprocessed, dark, reference, and corrected image on the same set of axes. Plot <tt>log10( count )</tt> on the vertical axis and intensity on the horizontal axis. Use a line plot instead of a bar chart for the histogram. |
Latest revision as of 14:08, 1 October 2018
Assignment details
This assignment has 2 parts:
- Part 1: Use the epifluorescence microscope you built to image fixed biological samples, and use the flat-field correction code you wrote for Assignment 2 to address non-uniform illumination;
- Part 2: Explore interesting calculations and considerations to guide your experimental design with fluorescence.
Submit your work on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment3.pdf. Here is a checklist of all things you have to turn in:
Here is a comprehensive list of what you need to turn in:
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