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• Physical optics and resolution
  ...uwenhoek and Robert Hooke made dramatic improvements in microscopes. Their work brought about many fundamental discoveries. Both men documented the existen * How does the probe work? What properties or events does it measure?
  15 KB (2,262 words) - 14:22, 6 September 2018
• Real electronics
  ...banana terminals in place. On new breadboards these are held away from the work surface by rubber or plastic feet on the backboard. On our breadboards thes
  9 KB (1,443 words) - 16:28, 20 October 2015
• Error analysis
  ...l. Fortunately, there always seems to be somebody with a bigger office who needs a couch. ...ch time you repeat a measurement, the incremental value added by your hard work diminishes. The tenth measurement is only about 11% as valuable as the firs
  24 KB (3,757 words) - 14:57, 3 September 2018
• Cellular microrheology
  ...he work of Mason ''et al.'' to 3T3 cells in particular can be found in the work of Tseng ''et al.'' <ref>[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC13023
  5 KB (750 words) - 18:34, 18 October 2013
• DNA Melting: Using the LockIn DNAMelter GUI
  ...ram again, and/or close it, re-open it, and run it again. If this does not work, consult an Instructor.
  10 KB (1,467 words) - 00:16, 8 November 2017
• DNA Melting: Using the Basic DNAMelter GUI
  ...d/or close MATLAB, re-open it, and run BasicMelter again. If this does not work, consult a TA or Instructor.
  5 KB (719 words) - 23:46, 7 November 2017
• Optics Bootcamp
  ...some exercises in the lab. The written problems will help you with the lab work. After you do the problems, come on by the lab. (Or do the problems in lab ... ray tracing and Snell's law to locate the image of the turtle. Show your work. <br>
  28 KB (4,857 words) - 20:09, 31 July 2017
• 20.109(S08)
  ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of
  3 KB (406 words) - 14:24, 5 June 2015
• 20.109(S08): TA notes for module 2
  #None: all work today is computer work. #*As before, ~1:20 dilution initiated between 8:30 and 9:30 am should work well.
  8 KB (1,223 words) - 16:04, 15 June 2015
• 20.109(S08): TA notes for module 1
  '''Prep Work''' *NB168 pCX-D3G+Linker -- This plasmid needs to be cut in three ways and each bkb purified!
  12 KB (1,868 words) - 16:04, 15 June 2015
• 20.109(S08):Transcript-level analysis (Day5)
  ...of Block Buffer to the plate. Wait another 60-90 minutes. In the meantime, work on your analysis (Part 3).
  14 KB (2,255 words) - 16:04, 15 June 2015
• 20.109(S08):Testing cell viability (Day3)
  ...of your labmates time.''' Reading the protocol in advance will help you work more quickly, and is strongly recommended. ...ls can basically be treated the same way. You and your partner may want to work in parallel, one on 2D prep and one on 3D prep. Unless otherwise stated, al
  18 KB (3,087 words) - 16:04, 15 June 2015
• 20.109(S08):Characterize protein expression (Day5)
  ... has 15 &mu;L of liquid in it. You might use the table below to guide your work. #Measure the absorbance of each sample at 595 nm. Work as quickly as you can, because the absorbance will continue to slowly chang
  12 KB (1,946 words) - 16:04, 15 June 2015
• 20.109(S08):Start-up biomaterials engineering (Day1)
  ... of the surrounding environment on cell phenotype. In particular, you will work with primary chondrocytes in both liquid and gel culture. The specific aspe ...many cells you need to initiate your cultures, in case extra animal tissue needs to be ordered. Per 3D sample, you will prepare 1 mL of alginate beads (thus
  5 KB (805 words) - 16:04, 15 June 2015
• 20.109(S08):Initiate cell culture (Day2)
  ...eir knee joints is more similar to that of humans. In this module, we will work with an ''in vitro'' culture model of cartilage-forming cells. ... time however you find useful (FNT assignment, notebook prep, or unrelated work).
  12 KB (2,006 words) - 16:04, 15 June 2015
• 20.109(S08):Preparing cells for analysis (Day4)
  ...eting your cells, clean your microfuge. You can finish setting up your RNA work area while the cells spin down. ... different from DNA in its stability. Consequently it is more difficult to work with RNA in the lab. It is not the techniques themselves that are difficult
  16 KB (2,751 words) - 16:04, 15 June 2015
• 20.109(S08):Protein-level analysis (Day6)
  ...king about the structure of cartilage for a bit, as promised on Day 4. Our work in this module has focused on chondrocytes themselves (viability and morpho ... diluted antibody per well. Incubate for 90 min (at room temperature), and work on Part 2 of today's protocol.
  9 KB (1,409 words) - 16:04, 15 June 2015
• 20.109(S08):Wrap-up analysis (Day7)
  ...nce they will undergo partly different manipulations. You may also want to work on two separate worksheets, one for each collagen type.
  7 KB (1,208 words) - 16:04, 15 June 2015
• 20.109(S08): Assignments
  ... semester in lab, but you will <font color = red>submit individual written work (lab report, portfolio, essay) and give individual journal club presentatio ...in the "for next time" section of each lab day. <font color = red> You can work with your lab partner, friends and teaching assistants on these assignments
  3 KB (394 words) - 14:24, 5 June 2015
• 20.109(S08):DNA engineering/Lab report
  ...figures, but if you do so, you must include the reference to the published work (e.g., the figure should not be downloaded from a web site). ...re used to detect homologous recombination in mammalian cells. The reader needs to know what the goal is: which plasmid did you set out to create? Part B w
  10 KB (1,670 words) - 14:24, 5 June 2015