Difference between revisions of "Assignment 3 Overview"
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{{Template:Assignment Turn In|message= Here is a comprehensive list of what you need to turn in: | {{Template:Assignment Turn In|message= Here is a comprehensive list of what you need to turn in: | ||
− | + | ('''as a team''') | |
# A figure with images of the stained cell samples with and without Cyto-D. | # A figure with images of the stained cell samples with and without Cyto-D. | ||
#* For each sample, create 1 figure with 5 panels. | #* For each sample, create 1 figure with 5 panels. | ||
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# Image profile | # Image profile | ||
#* For one reference, dark and cell image set, plot an intensity profile across the same diagonal. You may also use a bead image, along with it's unique reference and dark images. The intensity of your three images should be on the same scale, i.e., 0 to 65,535 or 0 to 1. Place all three profiles on a single set of axes for comparison. (Use the <tt>improfile</tt> command in MATLAB.) | #* For one reference, dark and cell image set, plot an intensity profile across the same diagonal. You may also use a bead image, along with it's unique reference and dark images. The intensity of your three images should be on the same scale, i.e., 0 to 65,535 or 0 to 1. Place all three profiles on a single set of axes for comparison. (Use the <tt>improfile</tt> command in MATLAB.) | ||
− | #Discussion | + | #Discussion ('''individually''') |
#* How did your beam expander design affect your images? | #* How did your beam expander design affect your images? | ||
#* What differences did you observe between the cells with and without CytoD? | #* What differences did you observe between the cells with and without CytoD? | ||
# Answers to all questions in [[Assignment 3, Part 2: experimental design with fluorescence|Assignment 3, Part 2]]. | # Answers to all questions in [[Assignment 3, Part 2: experimental design with fluorescence|Assignment 3, Part 2]]. | ||
− | }} | + | }} ('''individually''') |
{{Template:Assignment 3 navigation}} | {{Template:Assignment 3 navigation}} |
Revision as of 00:10, 25 January 2018
Assignment details
This assignment has 2 parts:
- Part 1: Use the epifluorescence microscope you built to image fixed biological samples, and use the flat-field correction code you wrote for Assignment 2 to address non-uniform illumination;
- Part 2: Explore interesting calculations and considerations to guide your experimental design with fluorescence.
Submit your work on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment3.pdf. Here is a checklist of all things you have to turn in:
Here is a comprehensive list of what you need to turn in: (as a team)
|
(individually)
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