Talk:20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)

IPC mutants (and primers) chosen by teams

T/R RED

• mutation: D132H
• forward primer:
• reverse primer:
• restriction enzyme:

W/F RED

• mutation: D57H
• forward primer: GCC GGC AGT CGA TGC TCA TGG CAA TGG AA
• reverse primer: TC ATT GAT CAT ATC CTG CAA TTC
• restriction enzyme: NaeI - GCCGGC

T/R ORANGE

• mutation: T78P
• forward primer:
• reverse primer:
• restriction enzyme:

W/F YELLOW

• mutation: D21A
• forward primer: 5' GCC GGC ATT CGA CAA GGC TGG GGA CGG CA
• reverse primer: 5' AAT GAG AAG GCT TCT TTG AAC TCT GC
• restriction enzyme: NaeI - GCCGGC

T/R GREEN

• mutation: D57H
• forward primer:
• reverse primer:
• restriction enzyme:

W/F GREEN

• mutation: T78P
• forward primer: 5' CCA GGG AAT GAA GGA CCC AGA CAG CGA AG 3'
• reverse primer: 5' TTT CTA GCC ATC ATA GTA AGA AAT TCA GGA AAG TAA ATC GTT CC 3'
• restriction enzyme: StyI - C/CWWGG

T/R BLUE

• mutation: D132H
• forward primer: 5' TAT CGA TGG TCA TGG CCA AGT AAA C 3'
• reverse primer: 5’ TCT GCT TCC CTT ATC ATT TC 3’
• restriction enzyme: None (no flap included)

W/F BLUE

• mutation: E30K
• forward primer: 5' CCA GGG CAC CAC AAA GAA ACT TGG CAC CG 3'
• reverse primer: 5' ATGGTGCCGTCCCCATCC 3'
• restriction enzyme: StyI-HF - C/CWWGG

T/R PINK

• mutation: D132H
• forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3'
• reverse primer: 5'TCT GCT TCC CTT ATC ATT TC 3'
• restriction enzyme: NaeI (GCC / GGC)

W/F PINK

• mutation: D132H
• forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3'
• reverse primer: 5'TCT GCT TCC CTT ATC ATT TC 3'
• restriction enzyme: NaeI (GCC / GGC)