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• Lab Manual: Optical Microscopy
  [[Category:Optical Microscopy Lab]] ...low contrast when imaged this way. Despite the limitations of bright field microscopy, many important discoveries were made with this simple method. Hooke was an
  23 KB (3,767 words) - 15:19, 10 January 2017
• Optical Microscopy Week 1: Build a brightfield microscope
  ====Optical microscopy==== ...u.com/articles/formulas/formulasconjugate.html Conjugate planes in optical microscopy] Includes transmitted and reflected (epi) illumination.
  9 KB (1,408 words) - 19:35, 16 September 2013
• Optical Microscopy Part 3: Resolution and Stability
  [[Category:Optical Microscopy Lab]] Congratulations on completing part 2. You made a functioning epifluorescence microscope. How cool is that?
  7 KB (1,168 words) - 18:12, 29 September 2016
• 20.309 development to-do list
  *Optical microscopy *Optical microscopy lab
  6 KB (738 words) - 18:50, 31 January 2013
• Locating objects in a fluorescent microscopic image
  * [[Epifluorescence microscopy]]
  3 KB (466 words) - 21:54, 4 January 2013
• Optical Microscopy Part 2: Fluorescence Microscopy
  [[Category:Optical Microscopy Lab]] In this part of the lab, you will add epifluorescence imaging capability to your microscope, make images of fluorescent samples,
  20 KB (3,262 words) - 22:06, 14 August 2017
• Physical optics and resolution
  [[Category:Optical Microscopy Lab]] Optical microscopy, involving visible light either transmitted through or reflected by the sam
  15 KB (2,262 words) - 14:22, 6 September 2018
• Optical Microscopy Part 3: Resolution, Stability, and Particle Tracking
  [[Category:Optical Microscopy Lab]] Congratulations on completing part 2. You made a functioning epifluorescence microscope. How cool is that?
  16 KB (2,559 words) - 22:47, 15 March 2017
• 20.109(F16):Data analysis (Day7)
  ... double-strand breaks in your H2AX assay samples, we will use fluorescence microscopy. ...ic wavelength. Typically, fluorescence microscopes used in biology are an epifluorescence type with a single light path (the objective) for excitation and emission d
  5 KB (901 words) - 16:39, 6 October 2016
• Assignment 1, Part 3: Building your transillumination microscope
  [[Category:Optical Microscopy Lab]] ...low contrast when imaged this way. Despite the limitations of bright field microscopy, many important discoveries were made with this simple method. Hooke was an
  38 KB (6,482 words) - 19:23, 6 September 2019
• Assignment 4: finding and measuring things
  [[Category:Optical Microscopy Lab]] ...ratulations on completing assignment 3. Now you can say you built your own epifluorescence microscope and used it to image mammalian cells. How cool is that?
  2 KB (244 words) - 14:49, 17 September 2018
• Assignment 3 Overview
  [[Category:Optical Microscopy Lab]] ... 3, Part 1: visualizing actin with fluorescence contrast|Part 1:]] Use the epifluorescence microscope you built to image fixed biological samples, and use the flat-fi
  3 KB (377 words) - 14:08, 1 October 2018
• 20.109(F17):Visualize and analyze data for sub-nuclear foci assay (Day7)
  ... double-strand breaks in your H2AX assay samples, we will use fluorescence microscopy. ...ic wavelength. Typically, fluorescence microscopes used in biology are an epifluorescence type with a single light path (the objective) for excitation and emission d
  9 KB (1,538 words) - 17:57, 11 October 2017
• 20.109(F18):Image sub-nuclear foci assay
  ... double-strand breaks in your H2AX assay samples, we will use fluorescence microscopy. ...ic wavelength. Typically, fluorescence microscopes used in biology are an epifluorescence type with a single light path (the objective) for excitation and emission d
  9 KB (1,538 words) - 15:34, 23 August 2018
• 20.109(F18):Image sub-nuclear foci assay (Day6)
  ... double-strand breaks in your H2AX assay samples, we will use fluorescence microscopy. ...ic wavelength. Typically, fluorescence microscopes used in biology are an epifluorescence type with a single light path (the objective) for excitation and emission d
  10 KB (1,563 words) - 15:38, 23 August 2018
• 20.109(F19):Complete CometChip experiment (Day5)
  ... double-strand breaks in your H2AX assay samples, we will use fluorescence microscopy. ...ic wavelength. Typically, fluorescence microscopes used in biology are an epifluorescence type with a single light path (the objective) for excitation and emission d
  12 KB (2,017 words) - 13:07, 26 September 2019
• 20.109(F20):M1D4
  ... double-strand breaks in your H2AX assay samples, we will use fluorescence microscopy. ...ic wavelength. Typically, fluorescence microscopes used in biology are an epifluorescence type with a single light path (the objective) for excitation and emission d
  11 KB (1,840 words) - 14:52, 16 September 2020
• 20.109(F21):M1D3
  ... fluorescent dye that will enable us to visualize double-strand breaks via microscopy. As a reminder, during the last laboratory session MCL-5 cells were treated ... double-strand breaks in your H2AX assay samples, we will use fluorescence microscopy.
  23 KB (3,807 words) - 20:07, 28 September 2021
• 20.109(S22):M1D8
  ...ited at 488 nm. To visualize TDP43 in CAD cells, we will use fluorescence microscopy. ...ic wavelength. Typically, fluorescence microscopes used in biology are an epifluorescence type with a single light path (the objective) for excitation and emission d
  17 KB (2,800 words) - 19:12, 3 March 2022
• 20.109(F22):M1D3
  ... fluorescent dye that will enable us to visualize double-strand breaks via microscopy. As a reminder, during the last laboratory session MEF cells were treated w ... double-strand breaks in your H2AX assay samples, we will use fluorescence microscopy.
  12 KB (1,945 words) - 20:38, 20 September 2022