DNA Melting: Simulating DNA Melting - Intermediate Topics

Write a function to compute f

Begin by writing a function that will compute $ \left . f \right . $ from the equation derived in class. This function must be in its own file called DnaFraction.m.

%Returns the fraction of dsDNA given total DNA concentration, temperature, Delta S, and Delta H
%Usage: f = DnaFraction(Ct, T, DeltaS, DeltaH)
function f = DnaFraction(Ct, T, DeltaS, DeltaH)

%Constants
R=8.3;

%first compute Ct * Keq
CtKeq = Ct * exp(DeltaS / R - DeltaH / (R * T));

%now compute f
f = (1 + CtKeq + sqrt(1 + 2 * CtKeq))/CtKeq;

Test the function

First, create a temperature vector. Then call DnaFraction with some reasonable parameters and plot the result. Units are calorie, mole.

t = [20:90] + 273;
f = DnaFraction(.1E-6, t, 304E3, 786);
plot(t,f)