Difference between revisions of "20.109(S24):Class data"

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Revision as of 18:07, 13 March 2024

20.109(S24): Laboratory Fundamentals of Biological Engineering

Sp24 banner image.png

Spring 2024 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       M1: Drug discovery        M2: Protein engineering        M3: Project design       


Module 1: Drug discovery

Please find uploaded data for Mod 1 in the Dropbox folder linked here.


Please find the SMM screen results here.


Please find the EMSA results here.


DSF results:

Replicate #1 Replicate #2 Replicate #3 Average Tm
TR Orange
DMSO 45.57 45.57 47.10 46.08
Small molecule #1 45.57 35.38 45.57 42.17
Small molecule #6 44.55 46.08 45.06 45.23
Small molecule #9 45.06 46.59 46.08 45.91
Small molecule #10 43.53 45.06 45.57 44.72
TR Yellow
DMSO 48.12 47.61 49.13 48.29
Small molecule #2 48.62 46.59 44.55 46.59
Small molecule #10 47.61 47.61 47.61 47.61
Small molecule #11 44.55 45.57 44.55 44.89
Small molecule #13 47.10 47.10 49.13 47.78
TR Green
DMSO 43.02 45.06 45.06 44.37
Small molecule #2 43.02 47.10 45.57 45.23
Small molecule #6 46.08 49.65 46.08 47.27
Small molecule #9 44.04 45.06 46.59 45.23
Small molecule #10 46.08 45.57 48.62 46.76
TR Blue
DMSO 47.11 45.08 44.06 45.42
Small molecule #1 48.64 48.64 44.06 47.11
Small molecule #2 46.60 47.11 43.56 45.76
Small molecule #4 46.60 44.57 43.56 44.91
Small molecule #10 47.62 44.57 44.06 45.42
TR Pink
DMSO 47.11 44.57 44.57 45.42
Small molecule #1 46.10 46.60 45.08 45.93
Small molecule #2 45.59 46.10 47.62 46.43
Small molecule #10 47.11 44.06 45.57 45.25
Small molecule #11 45.08 44.06 44.06 44.40


Replicate #1 Replicate #2 Replicate #3 Average Tm
WF Red
DMSO 47.05 44.51 35.86 42.47
Small molecule #3 45.53 46.55 46.04 46.04
Small molecule #4 46.55 47.05 45.53 46.38
Small molecule #5 43.49 47.06 46.55 45.70
Small molecule #8 45.02 46.55 45.53 45.70
WF Orange
DMSO 44.02 45.55 46.05 45.21
Small molecule #1 43.09 46.56 46.56 45.40
Small molecule #2 44.53 45.04 44.53 44.77
Small molecule #10 45.04 46.56 45.04 45.55
Small molecule #13 46.05 44.53 44.53 45.04
WF Yellow
DMSO 43.50 46.04 45.53 45.02
Small molecule #2 44.52 46.04 45.53 45.36
Small molecule #10 44.52 46.04 48.59 46.38
Small molecule #11 46.04 45.02 47.57 46.21
Small molecule #13 44.01 43.50 45.02 44.18
WF Green
DMSO 45.04 43.51 43.00 43.85
Small molecule #2 46.05 46.05 46.56 46.22
Small molecule #9 46.05 45.04 46.05 45.71
Small molecule #10 45.04 45.04 45.04 45.04
Small molecule #13 44.53 47.07 44.54 45.38
WF Blue
DMSO 46.04 44.52 43.50 44.685
Small molecule #5 45.02 43.50 45.02 45.70
Small molecule #9 47.06 47.06 45.02 46.38
Small molecule #10 48.08 45.02 44.01 45.70
Small molecule #13 45.53 43.50 44.01 44.35
WF Pink
DMSO 44.01819 46.46263 45.54488 45.3419
Small molecule #5 46.05378 45.03598 47.58047 46.22341
Small molecule #9 45.03598 46.56268 45.54488 45.71451
Small molecule #10 44.01819 46.56268 44.52709 45.0360
Small molecule #13 44.01819 44.52709 46.05378 44.86635
WF Purple
DMSO 47.05 43.50 42.99 44.51
Small molecule #5 45.53 47.06 45.53 46.04
Small molecule #9 42.98 46.04 48.59 45.87
Small molecule #10 44.51 45.02 46.55 45.36
Small molecule #13 46.04 43.50 46.04 45.19


Replicate #1 Replicate #2 Replicate #3 Average Tm
WF Teal
DMSO 45.54 45.54 43.00 44.69
Small molecule #1 47.07 46.05 44.53 45.88
Small molecule #2 44.53 45.04 45.54 45.04
Small molecule #10 46.56 46.56 47.07 46.73
Small molecule #11 47.07 46.56 44.53 46.05

Module 2: Protein engineering

Please find uploaded data for Mod 2 in the Dropbox folder linked [here].

T/R

Team Peptide sequence DNA sequence for peptide
TR orange DCCDCC GAC TGT TGC GAT TGT TGC
TR yellow CCGCCGCC TGC TGT GGC TGT TGT GGC TGT TGC
TR green CGCGGHGH TGC GGA TGT GGA GGT CAT GGT CAC
TR blue GCCGCCG GGA TGT TGT GGA TGC TGT GGC
TR pink GCHGCH GGT TGT CAC GGT TGT CAC

W/F

Team Peptide sequence DNA sequence for peptide
WF red
WF orange
WF yellow
WF green
WF blue
WF teal
WF pink
WF purple Asp - Cys - Cys - Asp - Cys - Cys - Asp GAC-TGT-TGC-GAT-TGC-TGT-GAC
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