Difference between revisions of "20.109(F07): M13.1 and MDS titers"

From Course Wiki
Jump to: navigation, search
(→‎Purple Team)
(→‎Red Team)
Line 104: Line 104:
  
 
===Red Team===
 
===Red Team===
−
*Team members:
+
*Team members:Rosa Alvarez, Vivian Hernandez
−
*Description of M13.1 phage:  
+
*Description of M13.1 phage:p9-YFP
−
*Description of MDS strain:  
+
*Description of MDS strain:MDS42
−
*Any notes about titers presented here:
+
*Any notes about titers presented here:no phage control has no plaques, M13K07 not grown at same time as M13.1
 +
====Plaque numbers====
 +
<center>
 +
{| border="1"
 +
|
 +
|phage = M13K07, strain = MG1655 
 +
|phage = M13K07, strain = MDS
 +
|phage = M13.1, strain = MG1655
 +
|phage = M13.1, strain = MDS
 +
|-
 +
| 10-6
 +
|fully lysed
 +
|ND
 +
|0
 +
|0
 +
|-
 +
| 10-8
 +
|~1000
 +
|ND
 +
|0
 +
|0
 +
|-
 +
| 10-10
 +
|~150
 +
|ND
 +
|0
 +
|0
 +
|}
 +
</center>
 +
====Titer====
 +
<center>
 +
{| border="1"
 +
|
 +
|phage = M13K07, strain = MG1655 
 +
|phage = M13K07, strain = MDS
 +
|phage = M13.1, strain = MG1655
 +
|phage = M13.1, strain = MDS
 +
|-
 +
| titer (PFU/ml)
 +
|
 +
|
 +
|
 +
|
 +
|}
 +
</center>
 +
 
 
===Pink Team===
 
===Pink Team===
 
*Team members:
 
*Team members:

Revision as of 14:49, 5 December 2007


20.109(F07): Laboratory Fundamentals of Biological Engineering

Macintosh HD-Users-nkuldell-Desktop-bldg10crop.png

Home        People        Schedule Fall 2007        Assignments        Lab Basics        OWW Basics       
Genome Engineering        Expression Engineering        Biomaterials Engineering              

All data on this page was collected by the MIT's Biological Engineering undergraduate students in the Fall 2007 as part of a laboratory class, 20.109. It represents the culmination of an ambitious phage redesign project that began at the start of the term. Redesigned phage were tested for phage titers on a "complete" E. coli genome (MG1655), and on the indicated MDS, described in [1]

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6
10-8
10-10

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml)

T/R lab

Green Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Purple Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here: no phage control has plaques

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6
10-8
10-10

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml)

Red Team

  • Team members:Rosa Alvarez, Vivian Hernandez
  • Description of M13.1 phage:p9-YFP
  • Description of MDS strain:MDS42
  • Any notes about titers presented here:no phage control has no plaques, M13K07 not grown at same time as M13.1

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6 fully lysed ND 0 0
10-8 ~1000 ND 0 0
10-10 ~150 ND 0 0

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml)

Pink Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Blue Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:M13K07 control not grown in parallel

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6 ~1000 ND ~1000 ~1500
10-8 16 ND 10 42
10-10

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml)

Yellow Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

W/F lab

Green Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Purple Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Red Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Pink Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Blue Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Yellow Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:
Retrieved from "http://engineerbiology.org/w/index.php?title=20.109(F07):_M13.1_and_MDS_titers&oldid=13726"