20.109(F07): Growth of phage materials
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Contents
Introduction
Protocols
In advance of this lab, a bacterial host (namely XL1-blue) was infected with the modified M13 phage called "3-12." Though the precise details of the 3-12 modification are still unpublished, it's known that it was derived from a screen to look for the changes in the phage p8 that enable the phage to bind a metal (Iridium). Today you will harvest the phage from the supernatant of the infected bacterial culture and then titer it.
Part 1: Phage purification
Part 2: Titering phage
DONE!
