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Create the page "Procedure" on this wiki!

• 20.109(S20): Research article
  ...e your audience is scientifically literate and somewhat familiar with each procedure. Remember that the methods should be divided into sub-sections that do not
  27 KB (4,110 words) - 16:15, 8 April 2020
• 20.109(F20):M1D1
  ...r cells, take the flask to your tissue culture hood to begin the splitting procedure.
  10 KB (1,598 words) - 23:22, 1 September 2020
• 20.109(F20):M1D2
  A plate seeded according to the procedure demonstrated in Part 1 was used to treat cells using the H₂O<sub *Provide a written overview / description of the the procedure used to prepare the coverslips for cell adhesion using poly-d-lysine (from
  11 KB (1,764 words) - 14:46, 10 September 2020
• 20.109(F20):Guidelines for working in the tissue culture facility
  #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
  7 KB (1,196 words) - 01:51, 15 July 2020
• 20.109(F20):M1D5
  ...ometChip experimental details, it is best to familiarize yourself with the procedure and assay. ...stration of this process. You should provide a written description of the procedure in your laboratory notebook!'''</font color>
  15 KB (2,489 words) - 22:13, 17 September 2020
• 20.109(F20):M1D4
  ===Part 2: Finish staining procedure for &gamma;H2AX assay=== *Provide a written overview / description of the the procedure used to prepare the &gamma;H2AX experiment for imaging (from the live demon
  11 KB (1,840 words) - 14:52, 16 September 2020
• 20.109(F20):M2D1
  ...stration of this process. You should provide a written description of the procedure in your laboratory notebook!'''</font color> *Provide a written overview / description of the the procedure used to prepare a restriction enzyme digest (from the live demonstration).
  25 KB (4,057 words) - 18:52, 15 October 2021
• 20.109(F20):M2D2
  ...ive demonstration of this process. You should include notes regarding the procedure in your laboratory notebook!'''</font color> #Repeat the elution procedure in Step #8 a total of three times.
  10 KB (1,645 words) - 15:53, 20 August 2020
• 20.109(F20):M2D3
  ...protein expression is visualized using SDS-PAGE. The protein purification procedure is completed with both an uninduced cell culture and an induced cell cultur ...ntaminants. (B) Aliquots are collected at different steps of concentration procedure to assess protein expression and purity.]]
  15 KB (2,402 words) - 21:45, 18 August 2020
• 20.109(F20):M3D3
  In the previous laboratory session, you reviewed the procedure used to generate the psgRNA_target plasmids. Today we will discuss how the ...hat each encode a different set of genes. Following the co-transformation procedure, a mixed population of cells exists as shown in the figure to the right: so
  17 KB (2,786 words) - 02:59, 20 August 2020
• 20.109(F20):M3D2
  ...into an expression plasmid. This is achieved using primers and PCR in the procedure described in Part 2. Before you review the approach used to insert the sgR
  17 KB (2,660 words) - 23:58, 30 July 2020
• 20.109(F20):Homework
  ...imental schematic focuses on the major steps used in for a single method / procedure. Though the focus is to highlight steps of a method, it is still best to o ... craft an experimental schematic that illustrates the protein purification procedure.
  21 KB (3,359 words) - 19:41, 21 September 2020
• 20.109(F20):Research article
  ...e your audience is scientifically literate and somewhat familiar with each procedure. Remember that the methods should be divided into sub-sections that do not
  27 KB (4,067 words) - 18:54, 25 July 2020
• 20.109(S21):M1D2
  ...hine is only able to count, and not sort subpopulations of cells, then the procedure is called flow cytometry, while cell sorting is called FACS.
  18 KB (2,876 words) - 15:37, 1 March 2021
• 20.109(S21):M1D3
  ... are prepared to take up DNA they are called competent. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
  11 KB (1,745 words) - 15:37, 1 March 2021
• 20.109(S21):M1D1
  ...dure called electroporation to get the DNA inside yeast cells. During this procedure yeast, specialized salt buffers, and DNA are loaded into a metal cuvette a
  21 KB (3,368 words) - 20:14, 18 February 2021
• 20.109(S21):M1D4
  Note: The procedure for DNA isolation at this scale (1-2mL) is commonly termed "mini-prep," whi
  16 KB (2,486 words) - 14:37, 3 March 2021
• 20.109(S21):M2D1
  ...ive demonstration of this process. You should include notes regarding the procedure in your laboratory notebook!'''</font color> *At several steps in the protein purification procedure, samples are collected that will be used later when protein expression and
  12 KB (1,935 words) - 20:31, 6 April 2021
• 20.109(S21):M2D2
  ...protein expression is visualized using SDS-PAGE. The protein purification procedure is completed with both an uninduced cell culture and an induced cell cultur ...ntaminants. (B) Aliquots are collected at different steps of concentration procedure to assess protein expression and purity.]]
  16 KB (2,534 words) - 11:50, 26 March 2021
• 20.109(S21):M2D5
  ...r cells, take the flask to your tissue culture hood to begin the splitting procedure.
  11 KB (1,878 words) - 23:14, 27 April 2021