Difference between revisions of "DNA Melting: Simulating DNA Melting - Intermediate Topics"
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Revision as of 20:55, 25 April 2008
Contents
Introduction
This tutorial will show you how to simulate the DNA melting experiment with Matlab. The DNA melting apparatus you will build produces two output voltages related to the sample temperature and fluorescence intensity. During an experimental run, these two voltages will be recorded periodically. At the end of this tutorial, you should be able to produce mock datasets that are similar to what you will record in the lab. You can use simulated datasets to help develop and debug your data analysis scripts.
It will be essential to have working data analysis scripts before you begin running experiments in the lab. When you have completed this tutorial, move on to the data analysis tutorial.
The procedure for creating the simulation is:
- Write a function to compute the equilibrium dsDNA contration vs. temperature
- Model sample temperature versus time during an experimental run
- Compute the RTD voltage from temperature vs. time data
- Add noise to the RTD signal
- Compute relative fluorescence intensity from temperature vs. time data
- Model photobleaching (optional)
- Scale the fluorescence intensity signal and add noise
- Reformat the data to match what is produced by the DNA melting LabView VI
You will not have to write any new code to complete this tutorial; however, you should open a Matlab window and follow along as you read. Code from this page can be copied and pasted into the Matlab command window. M-files are available in the course locker.
If you prefer to work in Python, please see this page.
Before you begin
- Read the DNA Melting Lab Manual.
- Review the DNA Melting Thermodynamics Notes.
- If you need a review of Matlab fundamentals, see Matlab Fundamentals tutorial.
Matlab skills
- Array arithmetic (element-by-element) operators (+, -, .*, ./)
- Functions declared in an m-file
- Conditionals
- Functions that take a variable number of arguments
- Numerical differentiation
- Plotting data
DNA annealing
We derived an expression for dsDNA concentration at a given temperature in class:
- $ K_{eq} = e^\left [\frac{\Delta S^{\circ}}{R} - \frac{\Delta H^{\circ}}{R T} \right ] $
- $ f = \frac{1 + C_T K_{eq} - \sqrt{1 + 2 C_T K_{eq}}}{C_T K_{eq}} $
Since this is a very complicated equation and we will use it many times, it makes sense to write a Matlab function.
dsDNA fraction function
The fraction of dsDNA is a function of 4 variables: ΔS°, ΔH°, temperature, and concentration. DnaFraction also takes an optional fifth argument for the gas constant so that different unit systems can be used. Using the element-by-element divide operator in the body of the function makes it possible for DnaFraction to accept either a single temperature argument or a vector of temperature values. (As a general rule, use the ./ operator instead of / unless you intend to do a matrix divide.)
The Matlab function nargin returns the number of arguments that the function was called with. Many Matlab programs use this function to handle variable numbers of arguments. If nargin returns 4 or less, R is set to the default value of 1.987. Otherwise, the value of R is set to the value supplied by the caller.
Remember that Matlab functions must be saved in your current directory or another directory in the Matlab path. The name of the file must be the function name appended with ".m".
<include src="http://web.mit.edu/~20.309/www/Matlab/DnaFraction.m" />
Ideal melting curve
Over the course of an interactive Matlab session, you may run several simulations with different parameters. Using a structure to keep the results of each simulation together is a good way to prevent the workspace from becoming disorganized. The struct function creates an empty structure. In the code below, a structure called ideal is created to hold the ideal melting curve data. (Since variables are dynamically allocated and typed in Matlab, the struct command is optional; however, explicitly creating the structure can make your code easier to read and better performing.)
Since DnaFraction accepts a vector temperature argument, an entire melting curve can be computed with a single call.
ideal = struct(); % create an empty structure to hold the results ideal.temperature = 0:99 + 273.15; % temperature axis in Kelvin: 100 points(0-99C) ideal.deltaS = -184; % cal/mol ideal.deltaH = -71E3; % cal/(mol K) ideal.concentration = 1E-6; % call DnaFraction function % arguments: concentration, temperature vector, delta S, delta H % (optional R argument not supplied) ideal.dsDnaFraction = DnaFraction(ideal.concentration, ideal.temperature, ... ideal.deltaS, ideal.deltaH); plot(ideal.temperature, ideal.dsDnaFraction); title('Ideal DNA Melting Curve'); xlabel('Temperature (K)'); ylabel('dsDNA Fraction');
Experiment with different values of ΔH° and ΔH° to see how the curve changes. You can use OligoCalc or another tool to estimate values for different DNA sequences.
One method of estimating Tm is to find the temperature where the dsDNA fraction equals 1/2. Another method is to differentiate the melting curve. Tm occurs at the peak of the derivative — where the dsDNA fraction is changing the fastest. (Why might you prefer one method to the other when estimating Tm from experimental data?)
Numerical differentiation
The fluorescence function fN is a sampled version of the continuous function f(T) such that fN = f(NΔT), where ΔT is the sampling interval (1° C, in this instance). One method for computing a numerical approximation to df/dT is the finite difference:
- $ {f'}_N = \frac{f_{N+1}-F_{N}}{\Delta T} $.
dF/dT is guaranteed to take on the value given by f'N at some point between NΔT and (N+1)ΔT; however, this happy coincidence can occur at any place in the interval. A simple but effective technique is to guess that equality occurs in the middle of the interval. This guess is close enough in most cases.
The code below computes the finite difference (ideal.dDsDnaFraction) and generates an associated temperature vector (ideal.dTemperature) with values centered between the original samples. Note that both vectors are one element shorter than the original data.
ideal.dTemperature = ideal.temperature(1:99) + 0.5; % create new temperature vector ideal.dDsDnaFraction = diff(ideal.dsDnaFraction); % take the difference plot(ideal.dTemperature, -1 * ideal.dDsDnaFraction); title('Derivative of Ideal DNA Melting Curve'); xlabel('Temperature (K)'); ylabel('Delta dsDNA Fraction');
In the previous section, the plot shows that the dsDNA fraction equals 0.5 at about 332.5 degrees. The peak of the derivative also occurs at the same temperature. (The curve is poltted inverted.)
Simulate an experimental run
Sample cooling
During each experimental run, the range of temperatures will be provided by natural cooling of the sample after it has been heated in a bath. At the beginning of an experimental run, the DNA + fluorescent dye sample is heated in a bath to around 90°C. Over a period of about 15 minutes, the sample cools to room temperature (about 20°C). Cooling can be approximated by an exponential function with a time constant of 3 minutes. The equation for temperature (in °K) versus time is:
- $ T(t) = 90 e ^ {-\frac{t}{180}} + 293 $
In Matlab:
simulationLength = 900; % 15 minute simulation sampleRate = 1; % 1 Hz sample rate sim1 = struct(); % create empty struct for sim resluts sim1.time = 0:(1./sampleRate):simulationLength; % create time vector (units: seconds) sim1.temperature = 90 * exp(-sim1.time ./ 180) + 293; % exponential cooling from 383K to 293K plot(sim1.time, sim1.temperature, 'r'); xlabel('Time (sec)'); ylabel('Temperature (K)'); title('Simulated Temperature vs. Time');
If you have any questions about the element-by-element division operator in Matlab (./) see:this page.
Model the RTD and voltage divider
The resistance of the RTD is given by the equation RRTD = 1000 + 3.85(T - 273). In the experimental apparatus, the RTD is hooked up as one element of a voltage divider with another resistor, R. The power supply is 15 volts. Thus, the output voltage is: VRTD = 15 * RRTD/(R + RRTD). The following code converts the temperature vector sim1.temperature into the equivalent RTD voltage measurements, assuming R = 20KΩ.
sim1.R_RTD = 1000 + 3.85 * (sim1.temperature - 273); % Compute RTD resistance in Ohms sim1.V_RTD = 15 * sim1.R_RTD ./ (20E3 + sim1.R_RTD); % Compute V_RTD in Volts plot(sim1.time, sim1.V_RTD, 'r'); xlabel('Time (sec)'); ylabel('RTD Voltage (V)'); title('Simulated RTD Voltage vs. Time');
Add some noise
You will certainly notice some noise on the signals you capture. (See: Where does electronic noise come from?.) Noise will be an extremely important (and annoying) factor in this experiment because the melting curve will be differentiated. Differentiation emphasizes noise. (Think about the frequency response of a differentiator.) In order to ensure that your data analysis script effectively handles the noise you are likely to observe, simulated random and impulse noise will be added to the signals.
To simulate noise, we can use Matlab's randn function, which generates normally distributed random numbers. The two arguments tell randn to create a vector of random samples with size 1xlength(V_RTD).
noise = 0.001 * randn(1,length(sim1.V_RTD)); % Create noise vector, standard deviation=1mV sim1.V_RTD = sim1.V_RTD + noise; % Add simulated noise to the voltage signal plot(sim1.time, sim1.V_RTD, 'r'); xlabel('Time (sec)'); ylabel('RTD Voltage (V)'); title('Simulated RTD Voltage with Noise vs. Time');
Model the sample fluorescence signal
Now that we have a simulated RTD voltage signal, the next step is to simulate the fluorescence signal. Remember that fluorescence intensity is proportional to the amount of dsDNA in the sample.
Create a dsDNA fraction (relative fluorescence) vs. time curve
Fluorescence intensity is assumed to be proportional to the concentration of dsDNA. The magnitude of fluorescence intensity is the highest when all of the DNA is annealed, which corresponds to a value of 1 on the dsDNA fraction curve.
% DnaFraction arguments: concentration,temp. vector, delta S, delta H (,optional R) sim1.dsDnaFraction = DnaFraction(1E-6, sim1.temperature, -184, -71E3); plot(sim1.time,sim1.dsDnaFraction) title('Fraction dsDNA vs. Time'); xlabel('Time (s)'); ylabel('dsDNA Fraction');
Scale the curve and add some noise
The output range of the transimpedance amplifier over the course of an experimental run can vary depending on several factors, including the amplifier gain, optical system gain, etc... A typical setup might produce values between, say, 0.5 and 7.2 Volts. The simulation code generates two random numbers to set the range and minimum value of the data. Your data analysis script will have to normalize the amplifier output voltage to a fractional value between 0 and 1. (What assumptions will you make about dsDNA concentration to do the normalization?)
In addition to Gaussian noise, the high gain amplifier can produce impulse noise. This can be caused by small static discharges, for example when a charged person sits down in a chair in the lab. It is a good idea to include some filtering for impulse noise in your data analysis script, in case your apparatus is susceptible to impulse noise.
Range = 5 + 4 * rand(); % Range between 5 and 9 Minimum = 2 * rand() - 1; % Minimum between +/- 1 sim1.V_f = Range * sim1.dsDnaFraction + Minimum; % Scale output noise = 0.01 * randn(1,length(sim1.V_f)); % Random noise, standard deviation=10mV sim1.V_f = sim1.V_f + noise; % Add random noise noise = rand(1,length(sim1.V_f)); % Uniformly distributed random numbers ImpulseNoiseProbability = 1 ./ 75; % Probability of impulse noise noise = 20 * (noise < ImpulseNoiseProbability) - 10; % Quantize impulse noise sim1.V_f = max(sim1.V_f, noise); % Add in impulse noise plot(sim1.time, sim1.V_f) title('Fluorescence Voltage vs. Time'); xlabel('Time (s)'); ylabel('Fluorescence Voltage (V)');
Reformat the data
When you import data from the LabView program that records DNA melting data, the format will be an N x 2 matrix with $ V_T $ values in column 1 and $ V_f $ in column 2. To put the simulated data in the same format, you can use Matlab's transpose operator (.') and the horizontal concatenation function horzcat:
simData = horzcat( sim1.V_RTD .', sim1.V_f .'); size(simData) ans = 901 2
Please see this page if you are uncertain about the .' operator.
Congratulations. You now have a simulated dataset that should be quite similar to what you measure in the lab.
Improving the model: photobleaching (optional)
Each time a SYBR Green molecule is excited by a photon, there is a small chance that it will be destroyed and permanently lose its ability to fluoresce. The effect of photobleaching on the measured fluorescence intensity can be significant. To demonstrate this, a model for photobleaching may be added to the simulation. In the model, a small, fixed percentage of the fluorophores are mathematically destroyed each time sample. This simple difference equation can be implemented with a for end loop.
- $ {\hat{f}}_{N} = B {\hat{f}}_{N-1} + f_{N} - f_{N-1} $
where,
- $ {\hat{f}}_N $ is the Nth sample of the bleached relative fluorescence,
- $ \left . B \right . $ is a bleaching constant equal to the fraction of fluorophore not destroyed per time sample, and
- $ \left . f_N \right . $ is the Nth sample of the dsDNA fraction (relative fluorescence) curve.
The fraction of fluorophores destroyed per time sample is set by the variable bleachingConstant. In the example code, the constant is set so that half of the fluorophores will be destroyed over the course of the simulated experimental run.
sim1.bleachingConstant = (0.5)^(1/900); % 1/2 of fluorphores fail over expmt. sim1.diffDsDnaFraction = diff(sim1.dsDnaFraction); % compute difference sim1.dsDnaFractionBleached(1) = 0.05; % set initial condition % for loop to compute difference equation for ii = 2:length(sim1.dsDnaFraction) sim1.dsDnaFractionBleached(ii) = sim1.dsDnaFractionBleached(ii - 1) * ... sim1.bleachingConstant + sim1.diffDsDnaFraction(ii - 1); end plot(sim1.time, sim1.dsDnaFractionBleached) title('Relative Fluorescence vs. Time with Bleaching'); xlabel('Time (s)'); ylabel('Relative Fluorescence');
The value of the first element of the vector sim1.dsDnaFractionBleached sets the initial condition for the difference equation — the relative fluorescence at the beginning of the simulation. Because SYBR green exhibits a small amount of intrinsic fluorescence when it is not bound to dsDNA, the initial value of sim1.dsDnaFractionBleached is set to a small number to model this effect.
Experiment with the bleaching constant and intrinsic fluorescence values to see how they distort the melting curve. Increasing the bleaching constant is equivalent to turning down the illumination intensity: fewer fluorphores will be destroyed in a given time. Unfortunately, the overall fluorescence intensity also decreases, necessitating higher amplifier gain and decreased signal to noise ratio. How will you decide on the optimal illumination and amplification levels in the lab? Can you use this model to correct for photobleaching effects in your data? How can you determine the bleaching constant experimentally?
If you would like to model photobleaching in your data analysis, follow the steps above for scaling the relative fluorescence signal, adding noise, and reformatting.
Data analysis advice
In outline, the steps to produce a melting curve from $ V_{RTD} $ and $ V_f $ are:
- Filter V_RTD and V_f to remove noise.
- Transform RTD voltage to temperature.
- Transform photodiode current to relative fluorescence.
- Ensure that the melting function is uniquely values by combining samples with identical temperature values.
- Differentiate the resulting function with respect to temperature.
Tm can be estimated directly from the melting curve or by finding the peak of the melting curve's derivative with respect to temperature. (Why would you prefer one method over the other?) Your data analysis script will have to convert the RTD and fluorescence intensity voltage signals into estimates of the temperature and dsDNA fraction.
Filtering
If you are not familiar with implementing digital filters in Matlab, review the Convolution and digital filtering in Matlab tutorial.
There are (at least)4 different convolution functions and 3 filtering functions in Matlab. Choosing the correct one is not always straightforward. Initial conditions Group delay
Uniquification
Sorting Binning
Numerical differentiation
diff function noise