Difference between revisions of "20.109(F08): The grafting parlour"
From Course Wiki
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==Primers to check Cln1 and Cln2:GFP strains== | ==Primers to check Cln1 and Cln2:GFP strains== | ||
− | ===Cln1=== | + | ===[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=CLN1 Cln1]= 1641bp=== |
+ | [[Image:CLN1 fwd.png|thumb|left| [http://www.ncbi.nlm.nih.gov/blast/bl2seq/wblast2.cgi BLAST2] seq for fwd primer and CLN1]] | ||
+ | [[Image:CLN1 rev.png|thumb|left| [http://www.ncbi.nlm.nih.gov/blast/bl2seq/wblast2.cgi BLAST2] seq for rev primer and CLN1]]<br> | ||
Forward: CCCT TTTCTCTCTATGCCCAT | Forward: CCCT TTTCTCTCTATGCCCAT | ||
*length = 21 | *length = 21 | ||
Line 57: | Line 59: | ||
*GC = 47.6 | *GC = 47.6 | ||
PCR product = 977bp<br> | PCR product = 977bp<br> | ||
− | PCR product+GFP = 1690bp | + | PCR product+GFP = 1690bp<br> |
===Cln2=== | ===Cln2=== |
Revision as of 02:52, 9 December 2008
Contents
NOTES FROM 11.11 meeting with the artist from THE GRAFTING PARLOUR
Please edit and add information that I didn't capture
Nkuldell 16:39, 11 November 2008 (EST)
Initial themes
- Form brings questions about content
- Computational approaches represent nature but biology holds in itself the reality of nature
- Art can tilt and sway perspective
- Interactive technology (video, telecommunication) are time base media
Framing questions
- How to look at the world through nature’s point of view?
- How can artwork change itself during a show?
- How can artwork change as it travels from gallery to gallery?
- We value the history of an object but can an object have traces/memories of itself and its history?
- Is the human desire to “fix time” immutable?
- We think of our cells as making up us but they have a life of their own as well (circadian pulsing of neurons every 23.5 hours w/o stimulus). What is the biological memory that cells have of a day? What do cells have to say to us?
- Galleries usually carefully manage light/moisture/temperature to inhibit bacteria growth on art but is this just a romantic notion of art by masters and is the intervention needed? Can unpredictable evolution/passage/change of art be part of art?
Art/Science examples we considered
- Eduardo Kac
- Transgenetic Alba bunny: use of animals as art? Research was done for science then made accessible through art
- Specimen of Secrecy About Marvelous Discoveries, pt 1
- Specimen of Secrecy About Marvelous Discoveries, pt2
- Hyunkoo Lee: animatus= skeletons from animated characters. Notable for its performance of science, that the artist makes apparent. Some eerie some playful examples.
NOTES: about microscope/display
from Lucy 12.08.08
- There are ways to convert a webcam into a microscope [1] but my guess is that the magnification is not great.
- Weather microscope or document camera, it depends on how wide a view will be captured. Here is
one that works with existing analog microscopes: [2]
- And here is more information on the document camera that I've been looking
at: [3]
Primers to check Cln1 and Cln2:GFP strains
Cln1= 1641bp
Forward: CCCT TTTCTCTCTATGCCCAT
- length = 21
- Tm = 57.6
- GC = 47.6
Reverse: CTAG ATGTTTGTAGGTGGGCA
- length = 21
- Tm = 57.6
- GC = 47.6
PCR product = 977bp
PCR product+GFP = 1690bp
Cln2
Forward: CACGGCATATTCTCCATTATC
- length: 21
- Tm = 57.2
- GC = 42.9
Reverse: GGTCTCTTTTTGGTACGTTTG
- length = 21
- Tm = 57.2
- GC = 42.9
PCR product = 804bp
PCR product+GFP = 1517bp