20.109(F08): Laboratory Fundamentals of Biological Engineering
| MODULE
|
DAY
|
DATE
|
LECTURER
|
TA
|
LAB
|
note
|
|
|
|
Thur Sept 4
|
NK]
|
|
Orientation
|
orientation day handout
|
|
|
| 1
|
1
|
Tue Sept 9
|
BE
|
MS
|
DNA engineering using PCR
|
NK lab notes, ANS lab notes
|
| 1
|
2
|
Thur Sept 11
|
BE
|
MS
|
Clean and cut DNA
|
NK lab notes, ANS lab notes
|
| 1
|
3
|
Tue Sept 16
|
BE
|
MS
|
Agarose gel electrophoresis
|
NK lab notes, ANS lab notes
|
| 1
|
4
|
Thur Sept 18
|
BE
|
MS
|
DNA ligation and bacterial transformation
|
NK lab notes, ANS lab notes
|
| 1
|
5
|
Tue Sept 23
|
BE
|
MS
|
Examine candidate clones
|
NK lab notes, ANS lab notes
|
| 1
|
6
|
Thur Sept 25
|
BE
|
MS
|
Restriction map and tissue culture
|
NK lab notes, ANS lab notes
|
| 1
|
7
|
Tue Sept 30
|
BE
|
MS
|
Lipofection
|
your first draft of the Mod 1 lab report is due before lab today
ANS lab notes
|
| 1
|
8
|
Thur Oct 2
|
BE
|
MS
|
FACS analysis
|
|
|
|
| 2
|
1
|
Tues Oct 7
|
NK]
|
BB
|
Protein engineering with PCR
|
your PowerPoint Pitch is due before lab today
M2D1 lecture notes
NK lab notes, ANS lab notes
|
| 2
|
2
|
Thur Oct 9
|
NK]
|
BB
|
Yeast transformation
|
M2D2 lecture notes
NK lab notes, ANS lab notes
|
| 2
|
3
|
Tues Oct 14
|
NK]
|
BB
|
Colony PCR, Journal article discussion
|
M2D3 lecture notes
NK lab notes, ANS lab notes
|
| 2
|
4
|
Thur Oct 16
|
NK]
|
BB
|
SDS-PAGE, screen for phenotypes
|
M2D4 lecture notes
NK lab notes, ANS lab notes
|
| 2
|
5
|
Tues Oct 21
|
NK]
|
BB
|
Probe Western, isolate RNA
|
guest lecture by Prof. Forest White about mass spec analysis of protein complexes. His slides are here
NK lab notes, ANS lab notes
|
| 2
|
6
|
Thurs Oct 23
|
NK]
|
BB
|
Journal Club I
|
optional visit to Abcam during lecture hour
|
| 2
|
7
|
Tues Oct 28
|
NK]
|
BB
|
cDNA synthesis and microarray
|
M2D7 lecture notes
NK lab notes, ANS lab notes
|
| 2
|
8
|
Thurs Oct 30
|
NK]
|
BB
|
Microarray data analysis
|
|
|
|
| 3
|
1
|
Tues Nov 4
|
AB]
|
LA
|
Phage growth for materials
|
your first draft of your research article is due before lab today
NK lab notes, ANS lab notes
|
| 3
|
2
|
Thurs Nov 6
|
AB]
|
LA
|
Phage nanowires
|
NK lab notes, ANS lab notes
|
|
|
|
|
|
Veteran's Day Break Nov 10,11
|
No lab Tuesday and Wednesday
|
|
|
| 3
|
3
|
Thur Nov 13
|
AB]
|
LA
|
Journal Club II
|
|
| 3
|
4
|
Tue Nov 18
|
AB]
|
LA
|
Phage by design
|
NK lab notes, ANS lab notes
|
| 3
|
5
|
Thur Nov 20
|
AB]
|
LA
|
Phage by design, pt 2
|
NK lab notes, ANS lab notes
|
|
|
|
|
|
Thanksgiving Break Nov
|
No lab or lecture all week
|
|
|
| 3
|
6
|
Tue Dec 2
|
AB]
|
LA
|
ECD assembly
|
NK lab notes, ANS lab notes
|
| 3
|
7
|
Thur Dec 4
|
AB]
|
LA
|
Oral presentations
|
|
| 3
|
8
|
Tue Dec 9
|
|
|
end of term celebration...
|
Lecture for class evaluations then lunch from noon-1:30 PM, no lab
|
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