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  • Assignment 8, Part 2: lock-in amplifier and temperature control
    This is Part 2 of [[Assignment 8 Overview| Assignment 8]]. ... transimpedance amplifier: an LM741. A good value for R<sub>sense</sub> is 10 &Omega;.
    17 KB (2,813 words) - 22:56, 7 November 2017
  • 20.109(S14):Preparing cells for analysis (Day4)
    ...easure protein content: move these to an eppendorf tube. The goal is about 10-15 (2-3 mm) beads per tube. #*For large beads (4-5 mm), you might use only 5-10 beads, and for very small beads (<1 mm), you might use 20 or more.
    24 KB (4,100 words) - 13:59, 29 July 2015
  • 20.109(F14): Mod 1 Day 3 Agarose gel electrophoresis
    | 10 &mu;L'''^''' #Incubate in the 50&deg;C water bath for 10 minutes, until the agarose is completely dissolved. Every few minutes, you
    13 KB (2,248 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 1 Day 1 DNA engineering using PCR
    ...0.6 inches (top, bottom, left and right) and you should change the text to 10 point Courier font. Courier font has a fixed letter width so all the lines ... ATGs you’re likely to find in 5700 bases. Do you think there will be 1? 10? 100? If there is more than one, how will you decide which ATG starts EGFP?
    23 KB (3,882 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 1 Day 2 Clean and cut DNA
    ...the membrane. '''EB''' is one solution we do know everything about. It’s 10 mM Tris pH 8.5. Since the DNA will solubilize at low salt and high pH, it e ... this assignment within ApE. Other program features may help you solve the assignment, but be sure not to "black box" any steps that you aren't sure you also und
    11 KB (1,780 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 1 Day 5 Examine candidate clones & tissue culture
    ...50 &mu;l of Solution III to each tube and immediately vortex each tube for 10 seconds with your vortex set at the highest setting. White clumps should ap ...rate the trypsin off the cells. Incubate the cells (“dry”) at 37° for 10 minutes, again using your timer to precisely time this incubation.
    20 KB (3,455 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 2 Day 1 Testing an engineered biological system
    |0:10 | 1:10 dilution
    15 KB (2,383 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 1 Day 4 DNA ligations, bacterial transformations and CometChip
    ... realize that even with high efficiency cells only 1 DNA molecule in about 10,000 is successfully transformed. ...droplets, and then incubate the reactions at room temperature for at least 10 minutes.
    12 KB (2,060 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 2 Day 3 Tools for system engineering
    *EXTRA CREDIT: you can get 2 points of extra credit on this FNW assignment by doing 2 small things in addition to your outline: **Strain NB462 (genotype: MC4100 ara+ (OmpC-lacZ) 10-25 envZ::KanR +pPL-PCBamp)
    28 KB (4,560 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 2 Day 5 Assessing re-tuned system
    #* 10 ul of sequencing primer NO296 (seq = TCG TCA ACC TCA TTT TGC GCC AG, this h ...d>'''Please note: this FNW assignment is due on Thursday, 10/30 or Friday, 10/31'''</font color>
    8 KB (1,382 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 2 Day 7 Readout of Protein
    ...apacity for each lane is usually 100 to 200 ug (that would be 20 ul of a 5-10 ug/ul protein preparation). Thus 1 ng represents a protein that is approxim ...ence of specific antibodies. Even the very best antisera have no more than 10% of their antibodies directed against a particular antigen. The quality of
    11 KB (1,712 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 3 Day 1 Growth of phage materials
    ...ched by a second, third, fourth round of panning. Ultimately anywhere from 10 to 1000 candidate sequences may remain from a starting pool of 1 billion [h ...d single-walled carbon nanotubes (SWNTs) ([http://openwetware.org/wiki/Doi:10.1038/nnano.2011.50 ]) by incorporating the DSPHTELP peptide into p8. They w
    16 KB (2,583 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 3 Day 3 TEM
    ...nWetWare for examples of research ideas in process. As part of a later FNT assignment, you will have to print out your wiki page specifying your topic, your rese <font color = blue>'''The written portions of this assignment are due on T/W, Dec 2nd & 3rd. Please pay special attention to the bolded b
    10 KB (1,709 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 3 Day 5 Solar cell testing
    ... this "sandwich" of materials you've made into a 100&deg;C drying oven for 10 minutes. (This step melts the Surlyn to the surface of the anode.) ..._module_of_a_different_color_is_still_biological_engineering. | reflection assignment]], either.
    6 KB (1,016 words) - 14:02, 29 July 2015
  • 20.109(F14): Mod 1 Day 4 DNA Ligation & Transformation
    ... realize that even with high efficiency cells only 1 DNA molecule in about 10,000 is successfully transformed. ...droplets, and then incubate the reactions at room temperature for at least 10 minutes.
    12 KB (2,028 words) - 14:02, 29 July 2015
  • 20.109(S15): Complete Western and prepare damaged DNA (Day3)
    ...ing costs $6-8 per reaction, which can add up if you need to examine, say, 10 or more candidates. Agarose gel electrophoresis, by comparison, costs perha #Add enough TBS-T to cover your membranes &ndash; between 10-15 mL should work, but you don't need to measure out this volume. Keep in m
    18 KB (2,985 words) - 17:15, 29 July 2015
  • 20.109(S15): Mod 3 Day 1 Growth of phage materials
    ...ched by a second, third, fourth round of panning. Ultimately anywhere from 10 to 1000 candidate sequences may remain from a starting pool of 1 billion [h ...d single-walled carbon nanotubes (SWNTs) ([http://openwetware.org/wiki/Doi:10.1038/nnano.2011.50 ]) by incorporating the DSPHTELP peptide into p8. They w
    16 KB (2,583 words) - 17:15, 29 July 2015
  • 20.109(S15): Mod 3 Day 1 Growth of phage materials (Day 1)
    ...ched by a second, third, fourth round of panning. Ultimately anywhere from 10 to 1000 candidate sequences may remain from a starting pool of 1 billion [h ...d single-walled carbon nanotubes (SWNTs) ([http://openwetware.org/wiki/Doi:10.1038/nnano.2011.50 ]) by incorporating the DSPHTELP peptide into p8. They w
    16 KB (2,577 words) - 17:15, 29 July 2015
  • 20.109(S15): TEM (Day 3)
    ...nWetWare for examples of research ideas in process. As part of a later FNT assignment, you will have to print out your wiki page specifying your topic, your rese ... a smaller diameter than those in the Y condition. The X wires had about a 10 nm? diameter, and the Y wires were about 2?-fold larger." Feel free to incl
    8 KB (1,323 words) - 17:15, 29 July 2015
  • 20.109(S15):16S PCR and paper discussion (Day3)
    ...are enough for the number of reactions you need to run, plus an additional 10%. In addition to the two DNA-containing reactions, you should prepare a no- ! Amount for 3 reactions + 10%
    22 KB (3,588 words) - 17:15, 29 July 2015

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