CM March28
From Course Wiki
Done this week
- Added a beam expander to the PMT path to make up for the beam shrinkage due to the relay lenses (-30mm and 200mm lens)
- Added mirrors, lenses and dichroics
- Positioned relay/beam expander lenses and adjusted the beam path to be the appropriate length based on these positions.
- Cleaned up the the microscope design by stabilizing various parts, decreasing distances where possible, straightening cage rods, etc.
- The galvos were not working, so we trouble shot the code and found that we were missing a "start" command at the end of the code. All fixed!
- In order to align the laser path to the galvos, we adjusted the height of the beam path and added a second mirror for additional flexibility.
To do next week
- Get a bright field image and align the laser so we can see it
- Position lenses in PMT beam path
- Install the pinhole and PMT and get a confocal image of a fluorescent slide!
- Install new laser and beamsplitter/photodiode system
Risks and Issues
- We're a little bit behind schedule!
- The laser-->galvos beam path isn't 100% straight
- What is the best way to normalize an image with the photodiode data?