Difference between revisions of "Talk:20.109(F17):Module 2"
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| + | ===Compiled class colorimetric data=== | ||
| + | [[Media:Fa17_CombinedColorimetricAssay_Acetate.xlsx|Acetate Compiled Class Data]] <br> | ||
| + | [[Media:Fa17_CombinedColorimetricAssay_Ethanol2.xlsx|Ethanol Compiled Class Data]] | ||
| + | |||
===Fermentation product and gene targeted:=== | ===Fermentation product and gene targeted:=== | ||
| Line 15: | Line 19: | ||
| adhE | | adhE | ||
| TTAACGCACTCGTAGAGCGTGTAAA | | TTAACGCACTCGTAGAGCGTGTAAA | ||
| − | | | + | | beginning of coding region |
| − | | | + | | non-template |
|- | |- | ||
|orange | |orange | ||
| Line 22: | Line 26: | ||
|pta-ack | |pta-ack | ||
|CTATGGCTCCCTGACGTTTT | |CTATGGCTCCCTGACGTTTT | ||
| − | | | + | |promoter region |
| − | | | + | |template strand |
|- | |- | ||
|yellow | |yellow | ||
| Line 29: | Line 33: | ||
|frdA | |frdA | ||
|GCTGTGGGATAAAAACAATCTGGAG | |GCTGTGGGATAAAAACAATCTGGAG | ||
| − | | | + | |minus 35 region |
| − | | | + | |template strand |
|- | |- | ||
|green | |green | ||
| Line 36: | Line 40: | ||
|LdhA | |LdhA | ||
|ttgtgctataaacggcgagtttcat | |ttgtgctataaacggcgagtttcat | ||
| − | | | + | |The middle of the gene |
| − | | | + | |Non template strand |
|- | |- | ||
|blue | |blue | ||
| Line 43: | Line 47: | ||
|pta | |pta | ||
|ttcacgacaacgttcaataatcat | |ttcacgacaacgttcaataatcat | ||
| − | | | + | |coding region |
| − | | | + | |non-template strand |
|- | |- | ||
|pink | |pink | ||
| Line 57: | Line 61: | ||
|adhE | |adhE | ||
|TTTACTAAAAAAGTTTAACATTATC | |TTTACTAAAAAAGTTTAACATTATC | ||
| − | |-35 region | + | |' -35 region' |
| − | |template | + | |'template' |
|- | |- | ||
|white | |white | ||
| Line 64: | Line 68: | ||
| adhE | | adhE | ||
|CTGATAATGTTAAACTTTTT | |CTGATAATGTTAAACTTTTT | ||
| − | | | + | |promoter |
| − | | | + | |non-template strand |
|} | |} | ||
| Line 82: | Line 86: | ||
| ack (indirectly, pta) | | ack (indirectly, pta) | ||
| GTTTTTTTAGCCACGTATCAATTAT | | GTTTTTTTAGCCACGTATCAATTAT | ||
| − | | | + | | promoter region of ack |
| − | | | + | | Nontemplate strand |
|- | |- | ||
|orange | |orange | ||
| Line 89: | Line 93: | ||
|ldhA | |ldhA | ||
|ATTCAACATCACTGGAGAAAGTCTT | |ATTCAACATCACTGGAGAAAGTCTT | ||
| − | | | + | |promoter |
| − | | | + | |template |
|- | |- | ||
|blue | |blue | ||
| Line 96: | Line 100: | ||
| ackA | | ackA | ||
| TTTTTAGCCACGTATCAATTAT | | TTTTTAGCCACGTATCAATTAT | ||
| − | | | + | | promoter region of ackA, starting at -32 |
| − | | | + | | nontemplate |
| + | |} | ||
| + | |||
| + | ====Instructor Data==== | ||
| + | |||
| + | {| border=1px | ||
| + | |'''Team''' | ||
| + | |'''Ethanol (E) or Acetate (A)''' | ||
| + | |'''Gene targeted by CRISPRi gRNA''' | ||
| + | |'''gRNA sequence (without tag at 3' end)''' | ||
| + | |'''Locus targeted (eg. beginning of gene, putative promoter, -35 region)''' | ||
| + | |'''Target template or nontemplate strand''' | ||
| + | |- | ||
| + | |Instructor | ||
| + | |Acetate | ||
| + | |aceEF | ||
| + | |GGAATAACCCatgtcagaacgtttc | ||
| + | |5' UTR region and beginning of coding region | ||
| + | |Template | ||
| + | |- | ||
| + | |Instructor | ||
| + | |Acetate | ||
| + | |adhE | ||
| + | |AATGCTCTCCTGATAATGTTAAAC | ||
| + | |Promoter and 5' UTR region right before coding region | ||
| + | |Nontemplate | ||
|} | |} | ||
Latest revision as of 16:20, 16 November 2017
Contents
Compiled class colorimetric data
Acetate Compiled Class Data
Ethanol Compiled Class Data
Fermentation product and gene targeted:
T/R
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target template or nontemplate strand |
| red | Acetate | adhE | TTAACGCACTCGTAGAGCGTGTAAA | beginning of coding region | non-template |
| orange | Ethanol | pta-ack | CTATGGCTCCCTGACGTTTT | promoter region | template strand |
| yellow | Ethanol | frdA | GCTGTGGGATAAAAACAATCTGGAG | minus 35 region | template strand |
| green | E | LdhA | ttgtgctataaacggcgagtttcat | The middle of the gene | Non template strand |
| blue | E | pta | ttcacgacaacgttcaataatcat | coding region | non-template strand |
| pink | Acetate | adhE | TTACTAAAAAAGTTTAACATTATCA | promoter region | template strand |
| purple | Acetate | adhE | TTTACTAAAAAAGTTTAACATTATC | ' -35 region' | 'template' |
| white | Acetate | adhE | CTGATAATGTTAAACTTTTT | promoter | non-template strand |
W/F
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target template or nontemplate strand |
| red | Ethanol | ack (indirectly, pta) | GTTTTTTTAGCCACGTATCAATTAT | promoter region of ack | Nontemplate strand |
| orange | Ethanol | ldhA | ATTCAACATCACTGGAGAAAGTCTT | promoter | template |
| blue | Ethanol | ackA | TTTTTAGCCACGTATCAATTAT | promoter region of ackA, starting at -32 | nontemplate |
Instructor Data
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target template or nontemplate strand |
| Instructor | Acetate | aceEF | GGAATAACCCatgtcagaacgtttc | 5' UTR region and beginning of coding region | Template |
| Instructor | Acetate | adhE | AATGCTCTCCTGATAATGTTAAAC | Promoter and 5' UTR region right before coding region | Nontemplate |
M2D5/M2D7: sequencing data
T/R
| Team | pgRNA sequencing |
| red | files |
| orange | files |
| yellow | files |
| green | files |
| blue | files |
| pink | files |
| purple | files |
| white | files |
W/F
| Team | pgRNA sequencing |
| red | files |
| orange | files |
| blue | files |
