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• 20.109(S15):AIV detection assay and analysis (Day7)
  | 95°C for 10 min ...etween 2/2/14 and 3/2/14. Click the link labeled with the tracking number 10-256046007.
  24 KB (3,931 words) - 17:15, 29 July 2015
• 20.109(S15):Begin Western protein analysis and choose system conditions (Day2)
  ...ence of specific antibodies. Even the very best antisera have no more than 10% of their antibodies directed against a particular antigen. The quality of ...apacity for each lane is usually 100 to 200 ug (that would be 20 ul of a 5-10 ug/ul protein preparation). Thus 1 ng represents a protein that is approxim
  27 KB (4,480 words) - 17:15, 29 July 2015
• 20.109(S15):Biotemplating on phage nanowires (Day 2)
  ...mplating, you may want to consult this review article: [http://dx.doi.org/10.1021/cm702152a] Sotiropoulou, Sofia, et al. "Biotemplated Nanostructured Ma #* Add ~10 dry ice chunks to the evaporation dish and let sit for 15 min. You can mea
  14 KB (2,281 words) - 17:15, 29 July 2015
• 20.109(S15):Cell preparation for DNA repair assays (Day4)
  ...diluted in OptiMEM (hereafter OM) as needed for all 12 transfections, plus 10%. ...ne experimental (B) – diluted in OM, enough for 6 transfections plus 10%, each.
  18 KB (2,885 words) - 17:15, 29 July 2015
• 20.109(S15):DNA cloning (Day4)
  ... realize that even with high efficiency cells only 1 DNA molecule in about 10,000 is successfully transformed. | 10
  16 KB (2,696 words) - 17:15, 29 July 2015
• 20.109(S15):DNA sequencing (Day5)
  ...penwetware.org/wiki/20.109%28S15%29:Primer_design_memo primer design memo] assignment. ...e A group; finally, start adding Soln. I to the B group when you reach the 10 min spin step for the A group. As one partner wraps up the B group, the oth
  16 KB (2,688 words) - 17:15, 29 July 2015
• 20.109(S15):Diagnostic primer design (Day2)
  [[Image:Screen Shot 2015-01-10 at 11.04.00 AM.png|thumb|450px|right|'''Schematic of avian influenza virus ...etware.org/wiki/20.109%28S15%29:Primer_design_memo#Content_Guidelines memo assignment] for details. Note that you are not yet required to write up the "overall s
  13 KB (2,073 words) - 17:15, 29 July 2015
• 20.109(S15):Growth of phage nanowires (Day 1)
  ...ched by a second, third, fourth round of panning. Ultimately anywhere from 10 to 1000 candidate sequences may remain from a starting pool of 1 billion [h ...d single-walled carbon nanotubes (SWNTs) ([http://openwetware.org/wiki/Doi:10.1038/nnano.2011.50 ]) by incorporating the DSPHTELP peptide into p8. They w
  16 KB (2,579 words) - 17:15, 29 July 2015
• 20.109(S15):Solar cell assembly (Day 4)
  ...recombined with the sensitizer or redox mediator (which occurs on a 10^-2, 10^-3second time frame). If instead, the electrons entered a metal, recombina ...ages on OpenWetWare for examples of research ideas in process. '''For this assignment, please submit a printed copy of your research page, making sure it defines
  13 KB (2,017 words) - 17:15, 29 July 2015
• 20.109(S15):Solar cell testing (Day 5)
  ... this "sandwich" of materials you've made into a 100°C drying oven for 10 minutes. (This step melts the Surlyn to the surface of the anode.) #Don't forget your final [[20.109(S15):20.109 Class Blog summary page | blog assignment]], either. Remember, you are required to complete a total of four blog post
  6 KB (985 words) - 17:15, 29 July 2015
• 20.109(F13): Mod 2 Day 6 Analysis & Planning 2
  ...ions into the sink and add enough TBS-T to cover your membranes -- between 10-15 mL should work, but you don't need to measure this out. Keep in mind tha #Shake your container for 10 min.
  17 KB (2,799 words) - 17:33, 29 July 2015
• 20.109(F13): Mod 2 Day 1 System Design
  In 2012, [http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048004 Bidkhori et al.] employed an ordinary differentia ...CLC and Intrinsic Resistance to TKI in Loss of PTEN Expression. PLoS ONE 7(10)]''']]
  26 KB (4,292 words) - 17:33, 29 July 2015
• 20.109(F13): Mod 3 Day 1 Growth of phage materials
  ...ched by a second, third, fourth round of panning. Ultimately anywhere from 10 to 1000 candidate sequences may remain from a starting pool of 1 billion [h ...d single-walled carbon nanotubes (SWNTs) ([http://openwetware.org/wiki/Doi:10.1038/nnano.2011.50 ]) while the p8#9 phage can bind thin gold films ([http:
  18 KB (2,861 words) - 17:33, 29 July 2015
• 20.109(F13): Mod 3 Day 2 Phage nanowires
  ...may want to consult this review article: [http://cdn-pubs.acs.org/doi/abs/10.1021/cm702152a?journalCode=cmatex Sotiropoulou, Sofia, et al. "Biotemplated #* Add ~10 dry ice chunks to the evaporation dish and let sit for 10 min. You can measure the temperature of this mixture, which should be ~ ne
  15 KB (2,422 words) - 17:33, 29 July 2015
• 20.109(F13): Mod 3 Day 3 TEM
  ...nWetWare for examples of research ideas in process. As part of a later FNT assignment, you will have to print out your wiki page specifying your topic, your rese ... a smaller diameter than those in the Y condition. The X wires had about a 10 nm? diameter, and the Y wires were about 2?-fold larger." Feel free to incl
  8 KB (1,330 words) - 17:33, 29 July 2015
• 20.109(F13): Mod 3 Day 5 Solar cell testing
  ... this "sandwich" of materials you've made into a 100°C drying oven for 10 minutes. (This step melts the Surlyn to the surface of the anode.) ..._module_of_a_different_color_is_still_biological_engineering. | reflection assignment]], either.
  6 KB (1,007 words) - 17:33, 29 July 2015
• 20.109(F13): Mod 2 Day 2 Mutation Analysis
  ##95° 10 min ##repeat steps 2-4 10 times
  18 KB (2,944 words) - 17:33, 29 July 2015
• 20.109(F13): Mod 2 Day 3 Prepare for LTS
  #Remove one 10 cm² plate of SKOV3 cells from the TC incubator. ...port | research article]] describing your Systems Biology study. For this assignment, you will start outlining and drafting the introduction to your report. Rec
  23 KB (3,661 words) - 17:42, 29 July 2015
• 20.109(S14):16S PCR and paper discussion (Day3)
  ...are enough for the number of reactions you need to run, plus an additional 10%. In addition to the two DNA-containing reactions, you should prepare a no- ! Amount for 3 reactions + 10%
  19 KB (3,108 words) - 13:58, 29 July 2015
• 20.109(S14):Phylogenetic and primer analyses (Day7)
  ...ass comparisons, along with composite trees for each bird population. (See assignment description for further guidance.) Most of the guidance about your data analysis is found in the assignment description [[20.109%28S14%29:Microbiome_summary |'''linked here'''.]]
  17 KB (2,730 words) - 13:58, 29 July 2015