Difference between revisions of "Optics Bootcamp"
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− | [[Image: 140730_OpticsBootcamp_3.jpg|thumb|300px|right| | + | [[Image: 140730_OpticsBootcamp_3.jpg|thumb|300px|right|Imaging apparatus with illuminator, object, lens, and CCD camera mounted on an optical rail.]] |
==Overview== | ==Overview== | ||
This lab exercise will introduce you to some of the optical components that you will use in the microscopy lab. You will build an apparatus that includes an LED illuminator, an object with precisely spaced markings, a lens, and a CCD camera. You will measure the image distance for several different object distances. Using the MATLAB Image Acquisition Tool, you will record images and use them to measure magnification. Finally, you will compare your measurements to the values given by the lens makers' formula derived in class. | This lab exercise will introduce you to some of the optical components that you will use in the microscopy lab. You will build an apparatus that includes an LED illuminator, an object with precisely spaced markings, a lens, and a CCD camera. You will measure the image distance for several different object distances. Using the MATLAB Image Acquisition Tool, you will record images and use them to measure magnification. Finally, you will compare your measurements to the values given by the lens makers' formula derived in class. | ||
− | == | + | ==Gather materials== |
+ | [[Image: 140729_OpticsBootcamp_02.jpg|thumb|right]] | ||
The first step is to gather the materials you need. The lists below include part numbers and descriptive names of all the components in the apparatus. It is likely that you will find some of the component names not-all-that-self-explanatory. Most of the parts come from a company called ThorLabs. If you have a question about one of the components, the [http://www.thorlabs.com ThorLabs website] can be very helpful. For example, if the procedure calls for an SPW602 spanner wrench and you have no idea what such an thing might look like, try googling the term: "thorlabs SPW602". You will find your virtual self just a click or two away from [http://www.thorlabs.com/thorproduct.cfm?partnumber=SPW602 a handsome photo and detailed specifications]. | The first step is to gather the materials you need. The lists below include part numbers and descriptive names of all the components in the apparatus. It is likely that you will find some of the component names not-all-that-self-explanatory. Most of the parts come from a company called ThorLabs. If you have a question about one of the components, the [http://www.thorlabs.com ThorLabs website] can be very helpful. For example, if the procedure calls for an SPW602 spanner wrench and you have no idea what such an thing might look like, try googling the term: "thorlabs SPW602". You will find your virtual self just a click or two away from [http://www.thorlabs.com/thorproduct.cfm?partnumber=SPW602 a handsome photo and detailed specifications]. | ||
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[[Image:20.390 Lab Map.png|Map of the 20.309 lab.]] | [[Image:20.390 Lab Map.png|Map of the 20.309 lab.]] | ||
− | There are more than 15,000 bits and pieces in the 20.309 lab | + | There are more than 15,000 bits and pieces in the 20.309 lab. Many of them look alike. It's important to learn your way around the lab so you don't spend a lot of time looking for things. Take a walk around the whole lab and just check things out. Spend at least five minutes poking around. Read the time machine poster near the Nikon microscope and check out the amazing Studley tool chest. See! Wasn't that worthwhile? |
Now go ahead and gather all the parts you will need. | Now go ahead and gather all the parts you will need. | ||
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* 1 x Calrad 45-601 power adapter for CCD | * 1 x Calrad 45-601 power adapter for CCD | ||
* 1 x ethernet cable connected to the lab station computer | * 1 x ethernet cable connected to the lab station computer | ||
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+ | ==Build the apparatus== | ||
{|class="wikitable" | {|class="wikitable" | ||
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|width="150"|[[Image: 140729_OpticsBootcamp_03.jpg|frameless|150px]] | |width="150"|[[Image: 140729_OpticsBootcamp_03.jpg|frameless|150px]] | ||
|width="150"|[[Image: 140729_OpticsBootcamp_09.jpg|frameless|150px]] | |width="150"|[[Image: 140729_OpticsBootcamp_09.jpg|frameless|150px]] |
Revision as of 05:37, 2 September 2014
Overview
This lab exercise will introduce you to some of the optical components that you will use in the microscopy lab. You will build an apparatus that includes an LED illuminator, an object with precisely spaced markings, a lens, and a CCD camera. You will measure the image distance for several different object distances. Using the MATLAB Image Acquisition Tool, you will record images and use them to measure magnification. Finally, you will compare your measurements to the values given by the lens makers' formula derived in class.
Gather materials
The first step is to gather the materials you need. The lists below include part numbers and descriptive names of all the components in the apparatus. It is likely that you will find some of the component names not-all-that-self-explanatory. Most of the parts come from a company called ThorLabs. If you have a question about one of the components, the ThorLabs website can be very helpful. For example, if the procedure calls for an SPW602 spanner wrench and you have no idea what such an thing might look like, try googling the term: "thorlabs SPW602". You will find your virtual self just a click or two away from a handsome photo and detailed specifications.
Orientation
There are more than 15,000 bits and pieces in the 20.309 lab. Many of them look alike. It's important to learn your way around the lab so you don't spend a lot of time looking for things. Take a walk around the whole lab and just check things out. Spend at least five minutes poking around. Read the time machine poster near the Nikon microscope and check out the amazing Studley tool chest. See! Wasn't that worthwhile?
Now go ahead and gather all the parts you will need.
Rails
Optical rails are a convenient way to mount components that have to slide back and forth. Components mount to clams that sit on the rail. A thumbscrew on the clamp locks it in position.
You will mount all of the components on an optical rail in order to easily adjust the distances between them. The rails are located **WHERE**. These are the rail parts that you will need:
- 1 x RLA1800 dovetail optical rails
- 4 x RC1 rail carriers
Lens tubes, cage components, and mounts
Posts, bases, and screws are located on the west credenza. Lens tubes and cage plates are on the center credenza. Cage rods are on the center bench.
- 1 x SM1L10 lens tube
- 1 x LCP01 cage plate (looks like an "O" in a square)
- 1 x LCP02 cage plate adapter (looks like an "X")
- 4 x ER1 cage assembly rod
- 1 x SM1RC lens tube slip ring
- 1 x CP02 cage plate
- 6 x SM1RR retaining rings
- 2 x SM2RR retaining rings
Screws and posts
- 6 x 1/4-20 x 5/16" cap screws
- 4 x PH2-ST post holders
- 4 x TR2 optical posts
- 4 x 8-32 set screws
- 1 x 1/4-20 set screw
- 8 x 4-40 set screws
Optics
- 1 x plano-convex f = 25 mm lens
- 1 x LB1811 biconvex f = 35 mm lens
Object
- 1 x R1DS1N 1951 USAF test target
Optoelectronics
- 1 x red LED
Tools
Here is a list of the tools you will need:
- 1 x SPW602 spanner wrench
- 1 x SPW801 adjustable spanner wrench
- 1 x 3/16 balldriver for 1/4-20 cap screws
- 1 x 9/64 balldriver
- 1 x 0.050" hex balldriver for 4-40 set screws
Things that should already be (and stay at) your lab station
- 1 x Manta CCD camera
- 1 x Calrad 45-601 power adapter for CCD
- 1 x ethernet cable connected to the lab station computer
Build the apparatus
Visualize, capture, and save images in Matlab
Examine images in Matlab
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Plot and discuss your results
- Repeat these measurements of $ S_o $, $ S_i $, $ h_o $, and $ h_i $ for several values of $ S_o $.
- Plot $ {1 \over S_i} $ as a function of $ = {1 \over f} - {1 \over S_o} $.
- Plot $ h_i $ as a function of $ h_o $.
- What sources of error affect your measurements?
- What is the uncertainty of your magnification measurement?
Optical microscopy lab
Code examples and simulations
- Converting Gaussian fit to Rayleigh resolution
- MATLAB: Estimating resolution from a PSF slide image
- Matlab: Scalebars
- Calculating MSD and Diffusion Coefficients