Difference between revisions of "CM March28"
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(Created page with "- Added a beam expander to the PMT path - Set the beam path from objective to beam path o Put lenses in o Adjusted distance to be perfect o Positioned 35mm and 250mm lenses - St...") |
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| − | + | '''Done this week''' | |
| − | + | * Added a beam expander to the PMT path to make up for the beam shrinkage due to the relay lenses (-30mm and 200mm lens) | |
| − | + | * Added mirrors, lenses and dichroics | |
| − | + | * Positioned relay/beam expander lenses and adjusted the beam path to be the appropriate length based on these positions. | |
| − | + | * Cleaned up the the microscope design by stabilizing various parts, decreasing distances where possible, straightening cage rods, etc. | |
| − | + | * The galvos were not working, so we trouble shot the code and found that we were missing a "start" command at the end of the code. All fixed! | |
| − | + | * In order to align the laser path to the galvos, we adjusted the height of the beam path and added a second mirror for additional flexibility. | |
| − | + | ||
| − | + | ||
| − | To do next week | + | '''To do next week''' |
| − | + | * Get a bright field image and align the laser so we can see it | |
| − | + | * Position lenses in PMT beam path | |
| − | + | * Install the pinhole and PMT and get a confocal image of a fluorescent slide! | |
| − | + | * Install new laser and beamsplitter/photodiode system | |
| − | + | ||
| − | + | ||
| − | - | + | '''Risks and Issues''' |
| + | * We're a little bit behind schedule! | ||
| + | * The laser-->galvos beam path isn't 100% straight | ||
| + | * What is the best way to normalize an image with the photodiode data? | ||
Latest revision as of 05:23, 4 April 2011
Done this week
- Added a beam expander to the PMT path to make up for the beam shrinkage due to the relay lenses (-30mm and 200mm lens)
- Added mirrors, lenses and dichroics
- Positioned relay/beam expander lenses and adjusted the beam path to be the appropriate length based on these positions.
- Cleaned up the the microscope design by stabilizing various parts, decreasing distances where possible, straightening cage rods, etc.
- The galvos were not working, so we trouble shot the code and found that we were missing a "start" command at the end of the code. All fixed!
- In order to align the laser path to the galvos, we adjusted the height of the beam path and added a second mirror for additional flexibility.
To do next week
- Get a bright field image and align the laser so we can see it
- Position lenses in PMT beam path
- Install the pinhole and PMT and get a confocal image of a fluorescent slide!
- Install new laser and beamsplitter/photodiode system
Risks and Issues
- We're a little bit behind schedule!
- The laser-->galvos beam path isn't 100% straight
- What is the best way to normalize an image with the photodiode data?
