Difference between revisions of "CM March28"
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(Created page with "- Added a beam expander to the PMT path - Set the beam path from objective to beam path o Put lenses in o Adjusted distance to be perfect o Positioned 35mm and 250mm lenses - St...") |
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Revision as of 05:13, 4 April 2011
- Added a beam expander to the PMT path - Set the beam path from objective to beam path o Put lenses in o Adjusted distance to be perfect o Positioned 35mm and 250mm lenses - Stabilized microscope, consolidated spacing, straightened cage rods basically cleaned up the design. - Added lenses, mirrors, dichroics in all locations - Got the galvos working and aligned - Aligned the laser beam
To do next week:
- get bright field image
- Align laser into field of view
- Get fluorescent image on camera in the place of the PMT
- Install the pinhole and PMT
- Get confocal image of fluorescent slide
- Install new laser and photodiode reading system
- Write code to normalize
