20.109(S23):Spring 2023 schedule

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20.109(S23): Laboratory Fundamentals of Biological Engineering

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Spring 2023 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       M1: Drug discovery        M2: Protein engineering        M3: Project design       


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not enough time to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times: Tuesday (T) and Thursday (R) 11 - 12 pm in 16-220
Laboratory section times: Tuesday (T) and Thursday (R) 1 - 5 pm or Wednesday (W) and Friday (F) 1 - 5 pm in 56-322

ABSENCE POLICY:
Absences from lecture: Attendance will be recorded for participation points throughout the semester. If absent, student is responsible for all information provided in lecture.
Absences from laboratory: Excused absences should be discussed with the Instructors as soon as possible. Because make-up laboratory time is not provided, attendance in another section may be required to complete the necessary experiments. Unexcused absences will result in a 1/3 of a letter grade deduction from the final grade on the major assignment for the module (for example, a B+ would become a B).

MODULE DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
T/W Feb 7/8 NLL
Lecture slides
Orientation and laboratory tour
M1D1 R/F Feb 9/10 [ AK]
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Orientation quiz
Homework due
M1D2 T/W Feb 14/15 [ AK]
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Homework due
M1D3 R/F Feb 16/17 [ AK]
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Homework due
T/W Feb 21/22 President's Day holiday
M1D4 R/F Feb 23/24 [ AK]
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Laboratory quiz
Homework due
M1D5 T/W Feb/Mar 28/1 [ AK]
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Homework due
Research talk due XX
M1D6 R/F Mar 2/3 [ AK]
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Homework due
M1D7 T/W Mar 7/8 [ AK]
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Laboratory quiz
Homework due
M2D1 R/F Mar 9/10 [ BCM]
Determine mutagenesis strategy
Homework due
M2D2 T/W Mar 14/15 [ RCM]
Perform site-directed mutagenesis
Homework due
M2D3 R/F Mar 16/17 [ BCM]
Sequence clones and transform into yeast cells
Homework due
Data Summary revision due XX
M2D4 T/W Mar 21/22 [ BCM]
Determine transporter mutation and expression
Laboratory quiz
Homework due
M2D5 R/F Mar 23/24 [ BCM]
IF analysis and prepare conditions for metal incubation experiment
Homework due
T/W Mar 28/29 - R/F Mar 30/31 Spring Break
T/W Apr 4/5 Journal article presentations
R/F Apr 6/7 Journal article presentations [ Blog post due] Sat, Apr 8 at 10 pm
M2D6 T/W Apr 11/12 [ BCM]
Analyze ICP-OES data and prepare for cytotoxicity experiment
Homework due
M2D7 R/F Apr 13/14 [ BCM]
Determine cell survival of yeast exposed to metal
Homework due
T/W Apr 18/19 Patriots' Day holiday
M2D8 R/F Apr 20/21 [ BCM]
Complete data analysis and organize Research article figures
Laboratory quiz
Homework due
T/W Apr 25/26
M3D1 R/F Apr 27/28 NLL
Brainstorm ideas for Research proposal presentation
Homework due
Research article due Mon, Nov 21 at 10 pm
Blog post due Tue, Nov 22 at 10 pm
M3D2 T/W May 2/3 AB
Pitch research proposal presentation ideas
M3D3 R/F May 4/5 BE Comm Lab Develop ideas for Research proposal presentation
Homework due
M3D4 T/W May 9/10 NLL
Participate in Research proposal peer reviews
Homework due
R/F May 11/12 Research proposal presentations [ Blog post due] Sat, May 13 at 10 pm
T May 16 Celebration lunch!