20.109(S23):Class data
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Revision as of 16:15, 23 March 2023 by Becky Meyer (Talk | contribs)
Contents
Module 1: Drug discovery
SDS-PAGE
T/R
Red Team: Media:TR Red S23M1D3.jpg
Orange Team: Media:TR Orange S23M1D3.jpg
Yellow Team: Media:TR Yellow S23M1D3.jpg
Green Team: Media:TR Green S23M1D3.jpg
Blue Team: Media:TR Blue S23M1D3.jpg
Pink Team: Media:TR Pink S23M1D3.jpg
Purple Team: Media:TR Purple S23M1D3.jpg
W/F
Dropbox Link : https://www.dropbox.com/sh/1p3sfs2487pkwr0/AAAAA-W0W6vPcXcfcp2nV2TPa?dl=0
BCA
W/F
Western Blot
W/F
Dropbox Link: https://www.dropbox.com/scl/fo/xlv4yz02oq2pfhka7mcmt/h?dl=0&rlkey=x1g0jqms054n0ycy3s2atbzrf
SMM
Module 2: Protein engineering
Mutagenesis
T/R
| Team | Mutation (eg. S301L) | Forward primer | Reverse primer |
| TR red | Y276C | TGGCAGATGTgTATTAACACTG | AGCGTCACTGAACTTTTTC |
| TR orange | Y276C | TTGGCAGATGtgtATTAACACTG | GCGTCACTGAACTTTTTC |
| TR yellow | Y276C | GGCAGATGTAcATTAACACTG | AAGCGTCACTGAACTTTTTC |
| TR green | Y352C | GATTGATTGGtgtGCTGATATTATTG | ATCTTTCTCCCTGTCGAC |
| TR blue | Y276C | TGGCAGATGTgTATTAACACTG | AGCGTCACTGAACTTTTTC |
| TR pink | D354C | TTGGTATGCTtgtATTATTGGTACTGG | TCAATCATCTTTCTCCCTG |
| TR purple | Y276C | TGGCAGATGTgTATTAACACTG | AGCGTCACTGAACTTTTTC |
T/R Sequencing [here]
W/F
| Team | Mutation (eg. S301L) | Forward primer | Reverse primer |
| WF red | V281C | TAACACTGCAtgTGCAGTTTCCCTTC | ATATACATCTGCCAAGCG |
| WF orange | I349C | GAGAAAGATGtgTGATTGGTATGCTGATATTATTG | CCTGTCGACCTTTTTTTAG |
| WF yellow | R405C | TTTCGTTTTGtgtGAAGTGTACTTCCG | CCGTCCAAAAACCCAATTAAAC |
| WF green | D271C | AAAGTTCAGTTGCGCTTGGCAGATGTATATTAACACTGCAGTTGC (AAAGTTCAGTtgcGCTTGGCAGATG) | TTCAATCTTGGATTACTACCTGTAGTTTCTCCGGTGTAAGGTGGA (TTCAATCTTGGATTACTACCTG) |
| WF blue | Y352I | GATTGATTGGattGCTGATATTATTGGTAC | ATCTTTCTCCCTGTCGAC |
| WF pink | D400C | ATTGGGTTTTTGtgCGGTTTCGTTTTG (TGGGTTTTTGtgcGGTTTCGTTTTG) | TAAACCTGTGTATGTACCGATAATCAACCACC (ATTAAACCTGTGTATGTACC) |
| WF purple | D350C | AAAGATGATTtgtTGGTATGCTGATATTATTGGTAC | CTCCCTGTCGACCTTTTTTTAG |
WF Sequencing here
ICP-OES data
T/R
Raw ICP-OES data [here]
| Team | Calculated Fe uptake | Calculated Cd update | Data file |
| TR red | |||
| TR orange | |||
| TR yellow | |||
| TR green | |||
| TR blue | |||
| TR pink | |||
| TR purple |
W/F
Raw ICP-OES data [here]
| Team | Calculated Fe uptake | Calculated Cd update | Data file |
| WF red | |||
| WF orange | |||
| WF yellow | |||
| WF green | |||
| WF blue | |||
| WF pink | |||
| WF purple |
